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The map label for this gene is pbpE

Identifier: 16080497

GI number: 16080497

Start: 3534118

End: 3535473

Strand: Reverse

Name: pbpE

Synonym: BSU34440

Alternate gene names: 16080497

Gene position: 3535473-3534118 (Counterclockwise)

Preceding gene: 16080504

Following gene: 16080496

Centisome position: 83.87

GC content: 45.13

Gene sequence:

>1356_bases
ATGAAGCAGAATAAAAGAAAGCATCTTCAGACGTTATTTGAGACATTAGGCGAAAAACATCAGTTTAACGGGACGGTTCT
GGCTGCGGAGGGTGGCGATATTTTATATCACCACTCTTTTGGCTATGCGGAAATGACGGAAAAACGCCCGTTGAAAACCA
ACTCTTTGTTTGAGTTAGCGTCTTTGTCAAAGCCTTTTACAGCATTGGGAATCATCTTGCTGGAGGAGAAGGGGATTCTT
GGCTATGAGGATAAAGTGGATCGCTGGCTGCCCGGTTTTCCGTATCAGGGCGTAACGATTCGGCATTTATTGAACCATAC
GTCAGGGCTTCCTGATTATATGGGATGGTTTTTTGCCAATTGGGATTCACACAAGATTGCGGTGAATCAGGATATTGTCG
ATATGCTGATGAATGAGGGGCTATCTGGTTATTTTGAACCGAATGAAGGCTGGATGTACAGCAATACGGGGTATGTGCTG
CTGGCGGTTATCATTGAAAAAGCATCTGGCATGAGCTATGCGGATTTTATAAAAACAAGTATTTTTTTACCGGCAGGCAT
GAATGAAACAAGAGTGTATAACAGGAGGCTTTCGCCTGAGCGAATTGATCATTATGCATATGGATATGTGTATGATGTAC
ATTCCGAAACATACGTCCTCCCTGATGAGCTGGAGGAAACGAACTATGTTGTGTATCTCGATGGCATACAGGGAGATGGG
ACGGTTAACTCTGTCACAAGCGATTTGTTTCGATTTGATCAGGCTTTATATCAGGACGATTTCATCAGCAAGGCTTCTAA
GGAATCTGCGTTTTCTCCAGTGCGCCTCAACAATGGAGAAACAATTGATTATGGCTTTGGCTGGGTGCTGCAAAACAGTC
CTGAAAAAGGGCGGATTGTCAGCCACAGCGGAGGATGGCCCGGCTATTCGACGATGATGATCCGATATATCGATCATCGT
AAAACGCTGATCTATTTGAGTAATAAGGAAGAGGATACTGAATATGAACAAGCGATTCTGAAGGCGGCGGAACATATTCT
ATTTGGCCAGCCATATGATGTCCCGGAACGTCCTGCTGATAAAAAGAAAAAAGCAATTGATACGGCGATATACAGCCGCT
ATGTTGGCAGCTACCTGTTGCAGGATGGGACGGCTGCACAGGTGACAACCGAGAATGAACGGCTTTATTTGGAGATCGCC
GGACAGCTGAGACTGGAGTTATTTCCTTCCTCGGAAACCCGTTTCTTTCTGCGAGCATTGTCTGTTGAAGTCGAGTTTAC
ATTAGGTGAGGATGCTGCGAAAAGCTTTATCCTCTATGAGGATGGCTCAGAGGAAGAAGCGGTCCGTACGAAGTAG

Upstream 100 bases:

>100_bases
TATATGTGATGGGTTAAAAAGGATCTCTAACTGAAGGATTTTTCAAAATATTTGAAACGTTAGTAGGTTAGTAACGTACA
GAGATATGGGAGGTGGAGGA

Downstream 100 bases:

>100_bases
AAAGGAATGAATTTGATGATCGGAATATTAGCCGGAATGGGGCCGAAATCGACCTCGCCGTTTATTGATAAGGTGATTGA
TTACTGTCAGAAGCTGTATG

Product: penicillin-binding protein 4*

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKQNKRKHLQTLFETLGEKHQFNGTVLAAEGGDILYHHSFGYAEMTEKRPLKTNSLFELASLSKPFTALGIILLEEKGIL
GYEDKVDRWLPGFPYQGVTIRHLLNHTSGLPDYMGWFFANWDSHKIAVNQDIVDMLMNEGLSGYFEPNEGWMYSNTGYVL
LAVIIEKASGMSYADFIKTSIFLPAGMNETRVYNRRLSPERIDHYAYGYVYDVHSETYVLPDELEETNYVVYLDGIQGDG
TVNSVTSDLFRFDQALYQDDFISKASKESAFSPVRLNNGETIDYGFGWVLQNSPEKGRIVSHSGGWPGYSTMMIRYIDHR
KTLIYLSNKEEDTEYEQAILKAAEHILFGQPYDVPERPADKKKKAIDTAIYSRYVGSYLLQDGTAAQVTTENERLYLEIA
GQLRLELFPSSETRFFLRALSVEVEFTLGEDAAKSFILYEDGSEEEAVRTK

Sequences:

>Translated_451_residues
MKQNKRKHLQTLFETLGEKHQFNGTVLAAEGGDILYHHSFGYAEMTEKRPLKTNSLFELASLSKPFTALGIILLEEKGIL
GYEDKVDRWLPGFPYQGVTIRHLLNHTSGLPDYMGWFFANWDSHKIAVNQDIVDMLMNEGLSGYFEPNEGWMYSNTGYVL
LAVIIEKASGMSYADFIKTSIFLPAGMNETRVYNRRLSPERIDHYAYGYVYDVHSETYVLPDELEETNYVVYLDGIQGDG
TVNSVTSDLFRFDQALYQDDFISKASKESAFSPVRLNNGETIDYGFGWVLQNSPEKGRIVSHSGGWPGYSTMMIRYIDHR
KTLIYLSNKEEDTEYEQAILKAAEHILFGQPYDVPERPADKKKKAIDTAIYSRYVGSYLLQDGTAAQVTTENERLYLEIA
GQLRLELFPSSETRFFLRALSVEVEFTLGEDAAKSFILYEDGSEEEAVRTK
>Mature_451_residues
MKQNKRKHLQTLFETLGEKHQFNGTVLAAEGGDILYHHSFGYAEMTEKRPLKTNSLFELASLSKPFTALGIILLEEKGIL
GYEDKVDRWLPGFPYQGVTIRHLLNHTSGLPDYMGWFFANWDSHKIAVNQDIVDMLMNEGLSGYFEPNEGWMYSNTGYVL
LAVIIEKASGMSYADFIKTSIFLPAGMNETRVYNRRLSPERIDHYAYGYVYDVHSETYVLPDELEETNYVVYLDGIQGDG
TVNSVTSDLFRFDQALYQDDFISKASKESAFSPVRLNNGETIDYGFGWVLQNSPEKGRIVSHSGGWPGYSTMMIRYIDHR
KTLIYLSNKEEDTEYEQAILKAAEHILFGQPYDVPERPADKKKKAIDTAIYSRYVGSYLLQDGTAAQVTTENERLYLEIA
GQLRLELFPSSETRFFLRALSVEVEFTLGEDAAKSFILYEDGSEEEAVRTK

Specific function: Probably involved in peptidoglycan modification during cortex synthesis

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family

Homologues:

Organism=Escherichia coli, GI1790593, Length=289, Percent_Identity=22.8373702422145, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17550934, Length=328, Percent_Identity=27.4390243902439, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17565630, Length=167, Percent_Identity=32.3353293413174, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPE_BACSU (P32959)

Other databases:

- EMBL:   L10629
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   A36908
- RefSeq:   NP_391324.1
- ProteinModelPortal:   P32959
- SMR:   P32959
- EnsemblBacteria:   EBBACT00000000376
- GeneID:   938615
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34440
- NMPDR:   fig|224308.1.peg.3450
- GenoList:   BSU34440
- GeneTree:   EBGT00070000031931
- HOGENOM:   HBG676736
- OMA:   SNTETAP
- PhylomeDB:   P32959
- ProtClustDB:   CLSK887921
- BioCyc:   BSUB:BSU34440-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471; SSF56601 PBP_transp_fold

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 51437; Mature: 51437

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQNKRKHLQTLFETLGEKHQFNGTVLAAEGGDILYHHSFGYAEMTEKRPLKTNSLFELA
CCCCHHHHHHHHHHHHCCCEECCCEEEEECCCCEEEEECCCCHHHHCCCCCCCCHHHHHH
SLSKPFTALGIILLEEKGILGYEDKVDRWLPGFPYQGVTIRHLLNHTSGLPDYMGWFFAN
HCCCCCHHEEEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHEEEC
WDSHKIAVNQDIVDMLMNEGLSGYFEPNEGWMYSNTGYVLLAVIIEKASGMSYADFIKTS
CCCCEEEECHHHHHHHHHCCCCCEECCCCCEEECCCCCEEEEEHHHHCCCCCHHHHHHHH
IFLPAGMNETRVYNRRLSPERIDHYAYGYVYDVHSETYVLPDELEETNYVVYLDGIQGDG
EEECCCCCHHHHHHCCCCHHHHHHEEEEEEEEECCCEEECCCCCCCCCEEEEEECCCCCC
TVNSVTSDLFRFDQALYQDDFISKASKESAFSPVRLNNGETIDYGFGWVLQNSPEKGRIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECEEEEECCCCCCCEEE
SHSGGWPGYSTMMIRYIDHRKTLIYLSNKEEDTEYEQAILKAAEHILFGQPYDVPERPAD
ECCCCCCCHHHHHEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCH
KKKKAIDTAIYSRYVGSYLLQDGTAAQVTTENERLYLEIAGQLRLELFPSSETRFFLRAL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECEEEEEEECCCCCEEEEEEE
SVEVEFTLGEDAAKSFILYEDGSEEEAVRTK
EEEEEEEECCHHHCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKQNKRKHLQTLFETLGEKHQFNGTVLAAEGGDILYHHSFGYAEMTEKRPLKTNSLFELA
CCCCHHHHHHHHHHHHCCCEECCCEEEEECCCCEEEEECCCCHHHHCCCCCCCCHHHHHH
SLSKPFTALGIILLEEKGILGYEDKVDRWLPGFPYQGVTIRHLLNHTSGLPDYMGWFFAN
HCCCCCHHEEEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHEEEC
WDSHKIAVNQDIVDMLMNEGLSGYFEPNEGWMYSNTGYVLLAVIIEKASGMSYADFIKTS
CCCCEEEECHHHHHHHHHCCCCCEECCCCCEEECCCCCEEEEEHHHHCCCCCHHHHHHHH
IFLPAGMNETRVYNRRLSPERIDHYAYGYVYDVHSETYVLPDELEETNYVVYLDGIQGDG
EEECCCCCHHHHHHCCCCHHHHHHEEEEEEEEECCCEEECCCCCCCCCEEEEEECCCCCC
TVNSVTSDLFRFDQALYQDDFISKASKESAFSPVRLNNGETIDYGFGWVLQNSPEKGRIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECEEEEECCCCCCCEEE
SHSGGWPGYSTMMIRYIDHRKTLIYLSNKEEDTEYEQAILKAAEHILFGQPYDVPERPAD
ECCCCCCCHHHHHEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCH
KKKKAIDTAIYSRYVGSYLLQDGTAAQVTTENERLYLEIAGQLRLELFPSSETRFFLRAL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECEEEEEEECCCCCEEEEEEE
SVEVEFTLGEDAAKSFILYEDGSEEEAVRTK
EEEEEEEECCHHHCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377