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The map label for this gene is eno

Identifier: 16080443

GI number: 16080443

Start: 3476555

End: 3477847

Strand: Reverse

Name: eno

Synonym: BSU33900

Alternate gene names: 16080443

Gene position: 3477847-3476555 (Counterclockwise)

Preceding gene: 16080444

Following gene: 16080441

Centisome position: 82.5

GC content: 45.55

Gene sequence:

>1293_bases
ATGCCATACATTGTTGATGTTTATGCACGCGAAGTATTAGACTCCCGCGGCAACCCAACAGTTGAAGTTGAAGTATATAC
AGAAACAGGAGCTTTCGGCCGCGCATTAGTGCCAAGCGGAGCTTCTACAGGTGAATACGAAGCGGTTGAGCTTCGTGACG
GCGACAAAGACCGTTACCTTGGAAAAGGCGTGTTAACAGCTGTTAACAACGTAAACGAAATCATTGCTCCAGAGCTTCTT
GGCTTTGATGTAACTGAACAAAACGCAATCGATCAGCTTTTAATCGAGCTTGACGGTACTGAAAACAAAGGCAAACTTGG
TGCGAACGCAATCCTTGGCGTATCTATGGCTTGTGCGCGTGCTGCTGCTGATTTCTTACAGATTCCTCTTTACCAATACC
TTGGAGGATTCAACTCAAAAACGCTTCCTGTACCGATGATGAACATCGTAAACGGCGGAGAGCATGCTGATAACAACGTG
GATATTCAAGAATTCATGATCATGCCTGTAGGTGCTCCTAACTTCCGTGAAGCACTTCGCATGGGCGCTCAAATCTTCCA
CAGCCTGAAATCAGTATTAAGCGCAAAAGGCTTAAACACTGCTGTAGGTGACGAAGGCGGATTCGCTCCAAACCTTGGTT
CTAACGAAGAAGCGCTTCAAACAATCGTTGAAGCAATCGAAAAAGCCGGCTTCAAACCTGGCGAAGAAGTTAAACTTGCT
ATGGATGCTGCATCTTCTGAGTTCTACAACAAAGAAGACGGCAAATACCATCTGTCTGGCGAAGGCGTTGTGAAAACATC
TGCTGAAATGGTTGACTGGTATGAAGAGCTTGTTTCTAAATACCCAATCATCTCGATCGAAGACGGACTTGACGAAAACG
ACTGGGAAGGCCACAAGCTTCTTACTGAGCGTCTTGGCAAAAAAGTTCAGCTTGTTGGTGACGACCTCTTCGTTACAAAC
ACGAAAAAACTTTCTGAAGGTATTAAAAACGGCGTAGGCAACTCTATCCTGATCAAAGTAAACCAAATCGGTACATTGAC
TGAAACATTCGATGCGATCGAAATGGCGAAACGCGCAGGCTACACAGCTGTTATCTCTCACCGTTCTGGTGAAACTGAAG
ACAGCACAATCGCTGACATCGCTGTGGCAACAAACGCAGGACAAATCAAAACAGGTGCTCCGTCTCGTACGGACCGTGTT
GCGAAATACAACCAGCTTCTTCGCATCGAAGATCAGTTGGCTGAAACTGCTCAATACCACGGTATCAACTCTTTCTACAA
CTTAAACAAGTAA

Upstream 100 bases:

>100_bases
CAACGTTATTAGACCTTCTTGGTGTTGAAAAACCGAAAGAAATGACAGGAACATCTTTAATTCAAAAATAATTTGAAAAT
CAAAAGGAGAGACAAAACTC

Downstream 100 bases:

>100_bases
TAAAAAGACCGCCGGGATTTTCTCTCGGCGGCTTTTTTATGCTTTCAGAGATCCAAATAAAGCCTTACCATATCGCGGCA
TTGAATCCCGTTTTCAAAAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPYIVDVYAREVLDSRGNPTVEVEVYTETGAFGRALVPSGASTGEYEAVELRDGDKDRYLGKGVLTAVNNVNEIIAPELL
GFDVTEQNAIDQLLIELDGTENKGKLGANAILGVSMACARAAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNV
DIQEFMIMPVGAPNFREALRMGAQIFHSLKSVLSAKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLA
MDAASSEFYNKEDGKYHLSGEGVVKTSAEMVDWYEELVSKYPIISIEDGLDENDWEGHKLLTERLGKKVQLVGDDLFVTN
TKKLSEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRV
AKYNQLLRIEDQLAETAQYHGINSFYNLNK

Sequences:

>Translated_430_residues
MPYIVDVYAREVLDSRGNPTVEVEVYTETGAFGRALVPSGASTGEYEAVELRDGDKDRYLGKGVLTAVNNVNEIIAPELL
GFDVTEQNAIDQLLIELDGTENKGKLGANAILGVSMACARAAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNV
DIQEFMIMPVGAPNFREALRMGAQIFHSLKSVLSAKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLA
MDAASSEFYNKEDGKYHLSGEGVVKTSAEMVDWYEELVSKYPIISIEDGLDENDWEGHKLLTERLGKKVQLVGDDLFVTN
TKKLSEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRV
AKYNQLLRIEDQLAETAQYHGINSFYNLNK
>Mature_429_residues
PYIVDVYAREVLDSRGNPTVEVEVYTETGAFGRALVPSGASTGEYEAVELRDGDKDRYLGKGVLTAVNNVNEIIAPELLG
FDVTEQNAIDQLLIELDGTENKGKLGANAILGVSMACARAAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNVD
IQEFMIMPVGAPNFREALRMGAQIFHSLKSVLSAKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLAM
DAASSEFYNKEDGKYHLSGEGVVKTSAEMVDWYEELVSKYPIISIEDGLDENDWEGHKLLTERLGKKVQLVGDDLFVTNT
KKLSEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVA
KYNQLLRIEDQLAETAQYHGINSFYNLNK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=52.0833333333333, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=50.9259259259259, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=50, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=50, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=45.1388888888889, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI169201331, Length=354, Percent_Identity=25.7062146892655, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI169201757, Length=354, Percent_Identity=25.7062146892655, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI239744207, Length=354, Percent_Identity=25.7062146892655, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=64.0845070422535, Blast_Score=530, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71995829, Length=436, Percent_Identity=51.1467889908257, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=436, Percent_Identity=51.1467889908257, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=43.298969072165, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=442, Percent_Identity=50.6787330316742, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=442, Percent_Identity=48.868778280543, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=442, Percent_Identity=48.868778280543, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=442, Percent_Identity=48.868778280543, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=442, Percent_Identity=50.6787330316742, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=50.3480278422274, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=50.3480278422274, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=50.3480278422274, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=50.3480278422274, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=50.4672897196262, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=50.4672897196262, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46582; Mature: 46451

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYIVDVYAREVLDSRGNPTVEVEVYTETGAFGRALVPSGASTGEYEAVELRDGDKDRYL
CCEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCHHHH
GKGVLTAVNNVNEIIAPELLGFDVTEQNAIDQLLIELDGTENKGKLGANAILGVSMACAR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNVDIQEFMIMPVGAPNFREALR
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHEEEECCCCCCHHHHHH
MGAQIFHSLKSVLSAKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDAASSEFYNKEDGKYHLSGEGVVKTSAEMVDWYEELVSKYPIISIEDGLDENDWEGHKL
EHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTERLGKKVQLVGDDLFVTNTKKLSEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDQLAETAQYH
CEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GINSFYNLNK
CCCCCCCCCC
>Mature Secondary Structure 
PYIVDVYAREVLDSRGNPTVEVEVYTETGAFGRALVPSGASTGEYEAVELRDGDKDRYL
CEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCHHHH
GKGVLTAVNNVNEIIAPELLGFDVTEQNAIDQLLIELDGTENKGKLGANAILGVSMACAR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNVDIQEFMIMPVGAPNFREALR
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHEEEECCCCCCHHHHHH
MGAQIFHSLKSVLSAKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDAASSEFYNKEDGKYHLSGEGVVKTSAEMVDWYEELVSKYPIISIEDGLDENDWEGHKL
EHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTERLGKKVQLVGDDLFVTNTKKLSEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDQLAETAQYH
CEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GINSFYNLNK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA