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The map label for this gene is opuCB

Identifier: 16080435

GI number: 16080435

Start: 3469184

End: 3469837

Strand: Reverse

Name: opuCB

Synonym: BSU33820

Alternate gene names: 16080435

Gene position: 3469837-3469184 (Counterclockwise)

Preceding gene: 16080436

Following gene: 16080434

Centisome position: 82.31

GC content: 47.86

Gene sequence:

>654_bases
ATGAATCAAATGATGACTTTTTTGCAAACGAACGGCGGAGAGCTGCTGTATAAAACAGGAGAGCATTTATATATTTCACT
CATAGCCGTTGTATTGGGCATTATCGTTGCAGTGCCGCTCGGCGTTGCTCTTACCAGAATGAAAAAAGGCGCAGGTGCGG
TTATCGGTTTCGTAAACATTGTGCAAACCCTGCCGAGTCTGGCGATTTTAGCCTTTTTTATTCCGCTTCTCGGCGTAGGA
AAAGTGCCTGCGATTGTCGCTTTATTTTTCTATTCGGTACTGCCGATCCTGCGCAATACGTATACCGGCATTAAAGGTGT
AAATAAAAACCTCCTGGAATCGGGGAAAGGAATTGGCATGACCGGCTGGGAGCAGATTCGGCTCGTTGAAATCCCGCTGG
CGATTCCCATCATCATGGCGGGGATCCGCACATCAACGATCTACCTAATTGGCTGGGCGACACTTGCGTCGTTTATCGGG
GGAGGCGGCCTCGGGGACTATATTTTTATCGGACTGAACCTATACCAGCCTGAATATATCATTGGCGGTGCCGTGCCTGT
CACAATTCTGGCAATTATTATTGATTATGTTCTGGCTGTGACAGAACGAAAGGTGACGCCGAAAGGCTTGCAAGGGATGA
AGGAAGTATCGTAA

Upstream 100 bases:

>100_bases
GTGACAAGAGCGAGCCTCGTTGATATCGTATACGATTCCATTTGGGGCGACGAGGAAAACCAGCTCATGACGATCTGACC
AGGGGAGGAGCTCTTTTAAG

Downstream 100 bases:

>100_bases
GGAGTTGGCACTGTTGAAAATGACAAAAATCAAATGGCTTGGCGCGTTTGCTCTCGTCTTTGTCATGCTGCTAGGCGGCT
GCTCTCTGCCGGGTCTAGGC

Product: glycine betaine/carnitine/choline/choline sulfate ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNQMMTFLQTNGGELLYKTGEHLYISLIAVVLGIIVAVPLGVALTRMKKGAGAVIGFVNIVQTLPSLAILAFFIPLLGVG
KVPAIVALFFYSVLPILRNTYTGIKGVNKNLLESGKGIGMTGWEQIRLVEIPLAIPIIMAGIRTSTIYLIGWATLASFIG
GGGLGDYIFIGLNLYQPEYIIGGAVPVTILAIIIDYVLAVTERKVTPKGLQGMKEVS

Sequences:

>Translated_217_residues
MNQMMTFLQTNGGELLYKTGEHLYISLIAVVLGIIVAVPLGVALTRMKKGAGAVIGFVNIVQTLPSLAILAFFIPLLGVG
KVPAIVALFFYSVLPILRNTYTGIKGVNKNLLESGKGIGMTGWEQIRLVEIPLAIPIIMAGIRTSTIYLIGWATLASFIG
GGGLGDYIFIGLNLYQPEYIIGGAVPVTILAIIIDYVLAVTERKVTPKGLQGMKEVS
>Mature_217_residues
MNQMMTFLQTNGGELLYKTGEHLYISLIAVVLGIIVAVPLGVALTRMKKGAGAVIGFVNIVQTLPSLAILAFFIPLLGVG
KVPAIVALFFYSVLPILRNTYTGIKGVNKNLLESGKGIGMTGWEQIRLVEIPLAIPIIMAGIRTSTIYLIGWATLASFIG
GGGLGDYIFIGLNLYQPEYIIGGAVPVTILAIIIDYVLAVTERKVTPKGLQGMKEVS

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane

COG id: COG1174

COG function: function code E; ABC-type proline/glycine betaine transport systems, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1788449, Length=176, Percent_Identity=39.7727272727273, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1788451, Length=192, Percent_Identity=30.7291666666667, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789033, Length=176, Percent_Identity=30.6818181818182, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OPUCB_BACSU (O34878)

Other databases:

- EMBL:   AF009352
- EMBL:   AL009126
- PIR:   D69670
- RefSeq:   NP_391262.1
- ProteinModelPortal:   O34878
- SMR:   O34878
- EnsemblBacteria:   EBBACT00000003704
- GeneID:   937105
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33820
- NMPDR:   fig|224308.1.peg.3388
- GenoList:   BSU33820
- GeneTree:   EBGT00070000031878
- HOGENOM:   HBG592565
- OMA:   GTIPVTI
- PhylomeDB:   O34878
- ProtClustDB:   CLSK885988
- BioCyc:   BSUB:BSU33820-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 23149; Mature: 23149

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1111f1d0)-; HASH(0x1273d890)-; HASH(0x12734590)-; HASH(0x1258b6d4)-; HASH(0x1261f90c)-; HASH(0x10da6610)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQMMTFLQTNGGELLYKTGEHLYISLIAVVLGIIVAVPLGVALTRMKKGAGAVIGFVNI
CCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VQTLPSLAILAFFIPLLGVGKVPAIVALFFYSVLPILRNTYTGIKGVNKNLLESGKGIGM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
TGWEQIRLVEIPLAIPIIMAGIRTSTIYLIGWATLASFIGGGGLGDYIFIGLNLYQPEYI
CCHHHEEEEEECHHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCCCCEEEEEEEECCCCEE
IGGAVPVTILAIIIDYVLAVTERKVTPKGLQGMKEVS
ECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCH
>Mature Secondary Structure
MNQMMTFLQTNGGELLYKTGEHLYISLIAVVLGIIVAVPLGVALTRMKKGAGAVIGFVNI
CCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VQTLPSLAILAFFIPLLGVGKVPAIVALFFYSVLPILRNTYTGIKGVNKNLLESGKGIGM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
TGWEQIRLVEIPLAIPIIMAGIRTSTIYLIGWATLASFIGGGGLGDYIFIGLNLYQPEYI
CCHHHEEEEEECHHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCCCCEEEEEEEECCCCEE
IGGAVPVTILAIIIDYVLAVTERKVTPKGLQGMKEVS
ECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377