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The map label for this gene is metN

Identifier: 16080325

GI number: 16080325

Start: 3361767

End: 3362591

Strand: Reverse

Name: metN

Synonym: BSU32730

Alternate gene names: 16080325

Gene position: 3362591-3361767 (Counterclockwise)

Preceding gene: 16080326

Following gene: 16080324

Centisome position: 79.77

GC content: 39.15

Gene sequence:

>825_bases
TTGAAAAAGCTATTTTTGGGTGCATTACTGCTTGTATTTGCAGGAGTTATGGCTGCCTGCGGTTCGAATAACGGCGCTGA
ATCCGGCAAGAAAGAAATTGTCGTTGCGGCAACAAAAACACCGCATGCGGAAATTTTAAAAGAAGCTGAACCATTGCTGA
AAGAAAAAGGCTATACGCTGAAAGTGAAAGTGCTTAGTGATTACAAAATGTACAATAAAGCTTTAGCTGATAAAGAAGTG
GACGCGAACTACTTCCAGCACATTCCTTACCTTGAGCAAGAAATGAAAGAAAACACAGATTACAAACTTGTGAATGCCGG
CGCTGTTCACTTAGAGCCATTCGGTATTTACTCTAAAACATACAAATCACTGAAAGACCTTCCAGACGGTGCGACAATCA
TTCTGACAAACAACGTTGCTGAACAAGGCCGTATGCTTGCAATGCTTGAAAACGCTGGATTAATCACTCTTGATTCTAAA
GTGGAAACAGTTGACGCAACATTGAAAGACATTAAGAAAAACCCGAAAAACCTTGAATTCAAAAAAGTAGCGCCTGAATT
AACGGCAAAAGCATATGAAAACAAAGAAGGAGACGCGGTCTTCATCAATGTAAACTATGCGATCCAAAATAAATTAAATC
CTAAAAAAGACGCAATTGAAGTAGAATCAACGAAAAACAACCCATACGCTAACATCATCGCAGTAAGAAAAGGCGAAGAA
GATTCTGCAAAAATCAAAGCGCTGATGGAAGTTCTTCACTCTAAAAAGATCAAAGACTTCATCGAGAAAAAATACGACGG
AGCTGTGCTTCCTGTATCTGAATAA

Upstream 100 bases:

>100_bases
TTCGTTGCTACTGTGTTTATTTTAATCATCGTGTTTATTATTCAAATCATCGGTGATCTAATAACAAATATTATAGACAA
ACGATAAGGGAGGATTTACA

Downstream 100 bases:

>100_bases
GACTGAAACCCCGGATGAGAATTCCGGGGTTTTTTTCGCGTATTTTCCTAAAAGCTGTTCATAATAAAAAGGAATGTTAA
ACAGATGGAGGGATTTTCTT

Product: methionine ABC transporter, substrate binding lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKKLFLGALLLVFAGVMAACGSNNGAESGKKEIVVAATKTPHAEILKEAEPLLKEKGYTLKVKVLSDYKMYNKALADKEV
DANYFQHIPYLEQEMKENTDYKLVNAGAVHLEPFGIYSKTYKSLKDLPDGATIILTNNVAEQGRMLAMLENAGLITLDSK
VETVDATLKDIKKNPKNLEFKKVAPELTAKAYENKEGDAVFINVNYAIQNKLNPKKDAIEVESTKNNPYANIIAVRKGEE
DSAKIKALMEVLHSKKIKDFIEKKYDGAVLPVSE

Sequences:

>Translated_274_residues
MKKLFLGALLLVFAGVMAACGSNNGAESGKKEIVVAATKTPHAEILKEAEPLLKEKGYTLKVKVLSDYKMYNKALADKEV
DANYFQHIPYLEQEMKENTDYKLVNAGAVHLEPFGIYSKTYKSLKDLPDGATIILTNNVAEQGRMLAMLENAGLITLDSK
VETVDATLKDIKKNPKNLEFKKVAPELTAKAYENKEGDAVFINVNYAIQNKLNPKKDAIEVESTKNNPYANIIAVRKGEE
DSAKIKALMEVLHSKKIKDFIEKKYDGAVLPVSE
>Mature_274_residues
MKKLFLGALLLVFAGVMAACGSNNGAESGKKEIVVAATKTPHAEILKEAEPLLKEKGYTLKVKVLSDYKMYNKALADKEV
DANYFQHIPYLEQEMKENTDYKLVNAGAVHLEPFGIYSKTYKSLKDLPDGATIILTNNVAEQGRMLAMLENAGLITLDSK
VETVDATLKDIKKNPKNLEFKKVAPELTAKAYENKEGDAVFINVNYAIQNKLNPKKDAIEVESTKNNPYANIIAVRKGEE
DSAKIKALMEVLHSKKIKDFIEKKYDGAVLPVSE

Specific function: Part of the ABC transporter complex metNIQ involved in methionine import. Binds the methionine and transfers it to the membrane-bound permease. It has also been shown to be involved in methionine sulfoxide transport (Probable)

COG id: COG1464

COG function: function code P; ABC-type metal ion transport system, periplasmic component/surface antigen

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpA lipoprotein family

Homologues:

Organism=Escherichia coli, GI1786396, Length=267, Percent_Identity=32.5842696629214, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1790093, Length=248, Percent_Identity=29.4354838709677, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METQ_BACSU (O32167)

Other databases:

- EMBL:   AL009126
- PIR:   B70020
- RefSeq:   NP_391152.1
- ProteinModelPortal:   O32167
- SMR:   O32167
- EnsemblBacteria:   EBBACT00000003355
- GeneID:   936716
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32730
- NMPDR:   fig|224308.1.peg.3278
- GenoList:   BSU32730
- GeneTree:   EBGT00050000001423
- HOGENOM:   HBG661773
- OMA:   KNNPYAN
- ProtClustDB:   CLSK887840
- BioCyc:   BSUB:BSU32730-MONOMER
- InterPro:   IPR004872

Pfam domain/function: PF03180 Lipoprotein_9

EC number: NA

Molecular weight: Translated: 30355; Mature: 30355

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFLGALLLVFAGVMAACGSNNGAESGKKEIVVAATKTPHAEILKEAEPLLKEKGYTL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEE
KVKVLSDYKMYNKALADKEVDANYFQHIPYLEQEMKENTDYKLVNAGAVHLEPFGIYSKT
EEEEECHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHCCCCEEEEECCEEEEECCCHHHHH
YKSLKDLPDGATIILTNNVAEQGRMLAMLENAGLITLDSKVETVDATLKDIKKNPKNLEF
HHHHHCCCCCCEEEEECCCHHCCCEEEEECCCCEEEECCCHHHHHHHHHHHHCCCCCCCH
KKVAPELTAKAYENKEGDAVFINVNYAIQNKLNPKKDAIEVESTKNNPYANIIAVRKGEE
HHHCHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCCC
DSAKIKALMEVLHSKKIKDFIEKKYDGAVLPVSE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MKKLFLGALLLVFAGVMAACGSNNGAESGKKEIVVAATKTPHAEILKEAEPLLKEKGYTL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEE
KVKVLSDYKMYNKALADKEVDANYFQHIPYLEQEMKENTDYKLVNAGAVHLEPFGIYSKT
EEEEECHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHCCCCEEEEECCEEEEECCCHHHHH
YKSLKDLPDGATIILTNNVAEQGRMLAMLENAGLITLDSKVETVDATLKDIKKNPKNLEF
HHHHHCCCCCCEEEEECCCHHCCCEEEEECCCCEEEECCCHHHHHHHHHHHHCCCCCCCH
KKVAPELTAKAYENKEGDAVFINVNYAIQNKLNPKKDAIEVESTKNNPYANIIAVRKGEE
HHHCHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCCC
DSAKIKALMEVLHSKKIKDFIEKKYDGAVLPVSE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377