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The map label for this gene is frlD

Identifier: 16080310

GI number: 16080310

Start: 3347051

End: 3347905

Strand: Reverse

Name: frlD

Synonym: BSU32570

Alternate gene names: 16080310

Gene position: 3347905-3347051 (Counterclockwise)

Preceding gene: 16080311

Following gene: 16080308

Centisome position: 79.42

GC content: 49.59

Gene sequence:

>855_bases
ATGAAATTGATTGCGGTTGGAGATAATGTTGTAGATTACTACCAGGATCAAGAAACATTTTACCCGGGAGGAAATGCCTT
AAACGTTGCTGTGCTTGCCAAGCGGCTCGGGCATGAGTCTTCCTATATCGGAATCGTCGGCAATGACGAAGCCGCGGCGC
ATCTGTTGAACGTTCTCAAGCTGGAGCAGGTCAATGCAGATTATATCCGCCAGGCGCACGGAGAAAACGGGATGGCGATT
GTCACCCTTGATGAACAGGGGGACCGCATTTTTGTCAGATCAAATAAAGGCGGCATTCAGTCCCGGCTTCGCCTCGCGTT
TCAGGAGAAAGATGTATCTTTTATCAGCGGGCATGATCTGCTGCATACGAGCGTGTACAGCAGGCTTGAGAACGATCTTC
CGCAGCTGTGCGGGCTGGTCCCGGTCTCATTTGATTTTTCAACCAATCGGGAGGATGATTATTTGAGGAGGGTCTGTCCT
TATGTGACGTATGCGTTTTTTTCAGGAAGCGATTTGAGTGAGTCGGAATGCGGTGAGCTTGCCAAAACAGCTCATGGGTA
CGGAGCCAAAATGGTTTGTATGACCCGAGGGGGCCAAGGAGCAATCCTGTCTGCGGGTGATCGAGTCTATCATCAGCCGA
TTGTGGAAGCTGATATAATAGATACACTTGGAGCGGGAGATTCTTTTATAGCAGGCTTTTTGACTGCCTTTTGCGTGAAG
CAGGATATAACGTATGCTTTGCGCCAGGCTGCTGAAACGGCTGCGAAAACTTGCGGTGTGTACGGAGCTTTCGGCTATGG
CTATCCTTATCGCTTGGAGGACGGGGGAAGCAGTGAAAAAACGAGAATACTATAA

Upstream 100 bases:

>100_bases
ATTTCAGCCGCGCCGATTATTATTCTATTTTTATTGATGCAGAAATATTTTGTCCGCGGGATTGCAAGCGGCAGTGTAAA
AGGATAAAGGAGGAAGCGCT

Downstream 100 bases:

>100_bases
GCAGTGTCTCGTTTTTTATGCCTGTTATGTGTAGGGGCTGTTGATGGTAAAAGTGACACGGTTTGTGTGCATCAAAAACA
ATGAGCAATAGATTGGCTGG

Product: fructoselysine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKLIAVGDNVVDYYQDQETFYPGGNALNVAVLAKRLGHESSYIGIVGNDEAAAHLLNVLKLEQVNADYIRQAHGENGMAI
VTLDEQGDRIFVRSNKGGIQSRLRLAFQEKDVSFISGHDLLHTSVYSRLENDLPQLCGLVPVSFDFSTNREDDYLRRVCP
YVTYAFFSGSDLSESECGELAKTAHGYGAKMVCMTRGGQGAILSAGDRVYHQPIVEADIIDTLGAGDSFIAGFLTAFCVK
QDITYALRQAAETAAKTCGVYGAFGYGYPYRLEDGGSSEKTRIL

Sequences:

>Translated_284_residues
MKLIAVGDNVVDYYQDQETFYPGGNALNVAVLAKRLGHESSYIGIVGNDEAAAHLLNVLKLEQVNADYIRQAHGENGMAI
VTLDEQGDRIFVRSNKGGIQSRLRLAFQEKDVSFISGHDLLHTSVYSRLENDLPQLCGLVPVSFDFSTNREDDYLRRVCP
YVTYAFFSGSDLSESECGELAKTAHGYGAKMVCMTRGGQGAILSAGDRVYHQPIVEADIIDTLGAGDSFIAGFLTAFCVK
QDITYALRQAAETAAKTCGVYGAFGYGYPYRLEDGGSSEKTRIL
>Mature_284_residues
MKLIAVGDNVVDYYQDQETFYPGGNALNVAVLAKRLGHESSYIGIVGNDEAAAHLLNVLKLEQVNADYIRQAHGENGMAI
VTLDEQGDRIFVRSNKGGIQSRLRLAFQEKDVSFISGHDLLHTSVYSRLENDLPQLCGLVPVSFDFSTNREDDYLRRVCP
YVTYAFFSGSDLSESECGELAKTAHGYGAKMVCMTRGGQGAILSAGDRVYHQPIVEADIIDTLGAGDSFIAGFLTAFCVK
QDITYALRQAAETAAKTCGVYGAFGYGYPYRLEDGGSSEKTRIL

Specific function: Catalyzes the phosphorylation of a range of fructosamines to fructosamine 6-phosphates

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family

Homologues:

Organism=Escherichia coli, GI1789775, Length=266, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87081971, Length=284, Percent_Identity=24.6478873239437, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FRLD_BACSU (O32153)

Other databases:

- EMBL:   AL009126
- PIR:   C70018
- RefSeq:   NP_391137.1
- ProteinModelPortal:   O32153
- SMR:   O32153
- EnsemblBacteria:   EBBACT00000002815
- GeneID:   936704
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32570
- NMPDR:   fig|224308.1.peg.3263
- GenoList:   BSU32570
- GeneTree:   EBGT00050000001880
- HOGENOM:   HBG655226
- OMA:   FEKVCPW
- PhylomeDB:   O32153
- ProtClustDB:   CLSK887832
- BioCyc:   BSUB:BSU32570-MONOMER
- InterPro:   IPR011611
- InterPro:   IPR002173

Pfam domain/function: PF00294 PfkB

EC number: NA

Molecular weight: Translated: 30986; Mature: 30986

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIAVGDNVVDYYQDQETFYPGGNALNVAVLAKRLGHESSYIGIVGNDEAAAHLLNVLK
CEEEEECCCHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
LEQVNADYIRQAHGENGMAIVTLDEQGDRIFVRSNKGGIQSRLRLAFQEKDVSFISGHDL
HHHCCHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHH
LHTSVYSRLENDLPQLCGLVPVSFDFSTNREDDYLRRVCPYVTYAFFSGSDLSESECGEL
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHH
AKTAHGYGAKMVCMTRGGQGAILSAGDRVYHQPIVEADIIDTLGAGDSFIAGFLTAFCVK
HHHHCCCCEEEEEEECCCCEEEEECCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
QDITYALRQAAETAAKTCGVYGAFGYGYPYRLEDGGSSEKTRIL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MKLIAVGDNVVDYYQDQETFYPGGNALNVAVLAKRLGHESSYIGIVGNDEAAAHLLNVLK
CEEEEECCCHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
LEQVNADYIRQAHGENGMAIVTLDEQGDRIFVRSNKGGIQSRLRLAFQEKDVSFISGHDL
HHHCCHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHH
LHTSVYSRLENDLPQLCGLVPVSFDFSTNREDDYLRRVCPYVTYAFFSGSDLSESECGEL
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHH
AKTAHGYGAKMVCMTRGGQGAILSAGDRVYHQPIVEADIIDTLGAGDSFIAGFLTAFCVK
HHHHCCCCEEEEEEECCCCEEEEECCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
QDITYALRQAAETAAKTCGVYGAFGYGYPYRLEDGGSSEKTRIL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377