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The map label for this gene is hom

Identifier: 16080279

GI number: 16080279

Start: 3314828

End: 3316129

Strand: Reverse

Name: hom

Synonym: BSU32260

Alternate gene names: 16080279

Gene position: 3316129-3314828 (Counterclockwise)

Preceding gene: 16080280

Following gene: 16080278

Centisome position: 78.66

GC content: 46.54

Gene sequence:

>1302_bases
TTGAAAGCGATTCGTGTAGGGCTTTTAGGTTTAGGTACCGTCGGAAGCGGTGTCGTTAAAATTATTCAAGACCATCAGGA
TAAGCTTATGCATCAGGTCGGCTGTCCGGTTACGATAAAAAAAGTGCTTGTAAAAGATTTAGAGAAAAAGAGAGAAGTAG
ATTTGCCGAAGGAAGTGCTCACGACAGAAGTGTATGATGTCATTGATGATCCAGATGTTGATGTCGTCATCGAGGTGATC
GGCGGCGTTGAACAGACAAAACAATATTTGGTCGACGCGCTGAGATCGAAAAAGCATGTTGTCACAGCAAACAAGGACTT
AATGGCTGTGTACGGCTCCGAGCTGCTTGCCGAAGCGAAGGAAAATGGATGCGATATCTACTTTGAAGCCAGTGTTGCCG
GCGGGATTCCGATTCTGCGCACGTTAGAGGAAGGCCTCTCATCAGATCGAATTACAAAAATGATGGGAATTGTGAACGGC
ACAACAAACTTTATCTTAACCAAAATGATCAAAGAGAAAAGCCCATACGAGGAAGTGCTCAAGGAAGCGCAGGATCTCGG
TTTTGCCGAAGCTGATCCGACTTCTGACGTGGAAGGGCTTGACGCCGCACGGAAAATGGCGATATTGGCGCGCCTCGGCT
TCTCGATGAACGTGGATCTGGAAGACGTCAAAGTAAAGGGGATCTCCCAAATTACAGACGAGGACATCAGCTTCAGCAAA
CGCCTCGGCTATACAATGAAGCTGATCGGGATTGCTCAGCGTGACGGCAGCAAAATCGAGGTCAGCGTACAGCCGACACT
GCTTCCTGACCATCATCCGCTTTCTGCTGTTCATAATGAGTTTAACGCTGTTTATGTATACGGCGAGGCTGTCGGTGAGA
CGATGTTCTACGGGCCGGGAGCCGGAAGCATGCCGACAGCGACATCCGTTGTTTCTGACCTCGTCGCTGTCATGAAAAAT
ATGCGCCTAGGTGTAACCGGCAACAGCTTTGTCGGACCGCAATATGAGAAAAACATGAAATCGCCGTCTGACATTTATGC
ACAGCAGTTTTTAAGAATTCATGTAAAAGATGAGGTTGGTTCATTCTCGAAAATTACATCTGTGTTCTCAGAGCGGGGCG
TGAGCTTTGAAAAAATCCTTCAGCTGCCAATTAAAGGCCATGATGAGTTAGCTGAAATCGTAATTGTCACACATCATACA
TCAGAAGCTGATTTCAGTGATATCCTGCAAAACCTAAATGATTTGGAAGTCGTTCAAGAAGTCAAAAGCACATATCGTGT
AGAAGGGAACGGTTGGAGCTAA

Upstream 100 bases:

>100_bases
AAACAGGAGCGGGCTTATATAATGTTTGTAACTTATATGTCTGTGTTGAGTTAACAATTGTCTTTTCCAAGAGGACAATC
AAAAGAAAGGTGGAGTTTTT

Downstream 100 bases:

>100_bases
TGTGGAAAGGACTTATCCATCAATATAAAGAATTTTTACCTGTAACAGATCAAACACCGGCGCTAACTTTACATGAAGGA
AACACACCTCTTATTCACCT

Product: homoserine dehydrogenase

Products: NA

Alternate protein names: HDH

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKAIRVGLLGLGTVGSGVVKIIQDHQDKLMHQVGCPVTIKKVLVKDLEKKREVDLPKEVLTTEVYDVIDDPDVDVVIEVI
GGVEQTKQYLVDALRSKKHVVTANKDLMAVYGSELLAEAKENGCDIYFEASVAGGIPILRTLEEGLSSDRITKMMGIVNG
TTNFILTKMIKEKSPYEEVLKEAQDLGFAEADPTSDVEGLDAARKMAILARLGFSMNVDLEDVKVKGISQITDEDISFSK
RLGYTMKLIGIAQRDGSKIEVSVQPTLLPDHHPLSAVHNEFNAVYVYGEAVGETMFYGPGAGSMPTATSVVSDLVAVMKN
MRLGVTGNSFVGPQYEKNMKSPSDIYAQQFLRIHVKDEVGSFSKITSVFSERGVSFEKILQLPIKGHDELAEIVIVTHHT
SEADFSDILQNLNDLEVVQEVKSTYRVEGNGWS

Sequences:

>Translated_433_residues
MKAIRVGLLGLGTVGSGVVKIIQDHQDKLMHQVGCPVTIKKVLVKDLEKKREVDLPKEVLTTEVYDVIDDPDVDVVIEVI
GGVEQTKQYLVDALRSKKHVVTANKDLMAVYGSELLAEAKENGCDIYFEASVAGGIPILRTLEEGLSSDRITKMMGIVNG
TTNFILTKMIKEKSPYEEVLKEAQDLGFAEADPTSDVEGLDAARKMAILARLGFSMNVDLEDVKVKGISQITDEDISFSK
RLGYTMKLIGIAQRDGSKIEVSVQPTLLPDHHPLSAVHNEFNAVYVYGEAVGETMFYGPGAGSMPTATSVVSDLVAVMKN
MRLGVTGNSFVGPQYEKNMKSPSDIYAQQFLRIHVKDEVGSFSKITSVFSERGVSFEKILQLPIKGHDELAEIVIVTHHT
SEADFSDILQNLNDLEVVQEVKSTYRVEGNGWS
>Mature_433_residues
MKAIRVGLLGLGTVGSGVVKIIQDHQDKLMHQVGCPVTIKKVLVKDLEKKREVDLPKEVLTTEVYDVIDDPDVDVVIEVI
GGVEQTKQYLVDALRSKKHVVTANKDLMAVYGSELLAEAKENGCDIYFEASVAGGIPILRTLEEGLSSDRITKMMGIVNG
TTNFILTKMIKEKSPYEEVLKEAQDLGFAEADPTSDVEGLDAARKMAILARLGFSMNVDLEDVKVKGISQITDEDISFSK
RLGYTMKLIGIAQRDGSKIEVSVQPTLLPDHHPLSAVHNEFNAVYVYGEAVGETMFYGPGAGSMPTATSVVSDLVAVMKN
MRLGVTGNSFVGPQYEKNMKSPSDIYAQQFLRIHVKDEVGSFSKITSVFSERGVSFEKILQLPIKGHDELAEIVIVTHHT
SEADFSDILQNLNDLEVVQEVKSTYRVEGNGWS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Escherichia coli, GI1786183, Length=278, Percent_Identity=26.2589928057554, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790376, Length=273, Percent_Identity=25.2747252747253, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322599, Length=102, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHOM_BACSU (P19582)

Other databases:

- EMBL:   M23217
- EMBL:   AL009126
- EMBL:   X04603
- PIR:   A31973
- RefSeq:   NP_391106.1
- ProteinModelPortal:   P19582
- SMR:   P19582
- EnsemblBacteria:   EBBACT00000003656
- GeneID:   936654
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32260
- NMPDR:   fig|224308.1.peg.3232
- GenoList:   BSU32260
- GeneTree:   EBGT00050000002519
- HOGENOM:   HBG527379
- OMA:   GIPIIEP
- ProtClustDB:   PRK06349
- BioCyc:   BSUB:BSU32260-MONOMER
- BRENDA:   1.1.1.3
- InterPro:   IPR002912
- InterPro:   IPR005106
- InterPro:   IPR016204
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000098

Pfam domain/function: PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3

EC number: =1.1.1.3

Molecular weight: Translated: 47495; Mature: 47495

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01042 HOMOSER_DHGENASE

Important sites: ACT_SITE 205-205 BINDING 105-105 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIRVGLLGLGTVGSGVVKIIQDHQDKLMHQVGCPVTIKKVLVKDLEKKREVDLPKEVL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHH
TTEVYDVIDDPDVDVVIEVIGGVEQTKQYLVDALRSKKHVVTANKDLMAVYGSELLAEAK
HHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHH
ENGCDIYFEASVAGGIPILRTLEEGLSSDRITKMMGIVNGTTNFILTKMIKEKSPYEEVL
CCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
KEAQDLGFAEADPTSDVEGLDAARKMAILARLGFSMNVDLEDVKVKGISQITDEDISFSK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHCCCHHHHHH
RLGYTMKLIGIAQRDGSKIEVSVQPTLLPDHHPLSAVHNEFNAVYVYGEAVGETMFYGPG
HCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEEEECHHCCEEEECCC
AGSMPTATSVVSDLVAVMKNMRLGVTGNSFVGPQYEKNMKSPSDIYAQQFLRIHVKDEVG
CCCCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHEEEHHHCC
SFSKITSVFSERGVSFEKILQLPIKGHDELAEIVIVTHHTSEADFSDILQNLNDLEVVQE
CHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHCCHHHHHHHH
VKSTYRVEGNGWS
HHHHEEECCCCCC
>Mature Secondary Structure
MKAIRVGLLGLGTVGSGVVKIIQDHQDKLMHQVGCPVTIKKVLVKDLEKKREVDLPKEVL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHH
TTEVYDVIDDPDVDVVIEVIGGVEQTKQYLVDALRSKKHVVTANKDLMAVYGSELLAEAK
HHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHH
ENGCDIYFEASVAGGIPILRTLEEGLSSDRITKMMGIVNGTTNFILTKMIKEKSPYEEVL
CCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
KEAQDLGFAEADPTSDVEGLDAARKMAILARLGFSMNVDLEDVKVKGISQITDEDISFSK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHCCCHHHHHH
RLGYTMKLIGIAQRDGSKIEVSVQPTLLPDHHPLSAVHNEFNAVYVYGEAVGETMFYGPG
HCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEEEECHHCCEEEECCC
AGSMPTATSVVSDLVAVMKNMRLGVTGNSFVGPQYEKNMKSPSDIYAQQFLRIHVKDEVG
CCCCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHEEEHHHCC
SFSKITSVFSERGVSFEKILQLPIKGHDELAEIVIVTHHTSEADFSDILQNLNDLEVVQE
CHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHCCHHHHHHHH
VKSTYRVEGNGWS
HHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3139660; 9384377; 3098560