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The map label for this gene is dapF

Identifier: 16080270

GI number: 16080270

Start: 3306040

End: 3306894

Strand: Reverse

Name: dapF

Synonym: BSU32170

Alternate gene names: 16080270

Gene position: 3306894-3306040 (Counterclockwise)

Preceding gene: 16080271

Following gene: 16080269

Centisome position: 78.44

GC content: 46.78

Gene sequence:

>855_bases
ATGAACTCATTTCGATTTACCAAAATGCACGGCCTGGGAAACAGTTATATTTATGTGAATCAATTTGAAGAACAGCTTCC
AGAAGAAAAACTGTCAGAGATTGCAATTCAGGTTTCTTCTGTTTATACAGGGATCGGATCCGACGGAATGATCCTGATAT
GCCCATCAGACCAAGCGCCTGTTAAAATGCGGATTTTTAATAATGACGGGTCTGAAGGGAAAAACTGCGGCAACGGCCTG
CGCTGCGTCGCAAAATACGCATATGAGCATAAGCTGGTTGAGGAAACATCTTTTCTGATTGAAACTCTCTCCGGGCTTGT
GAAAGCGGAGGTTCAAGTCGAAAACGGAAAAGTGAATGTGGTCACTGTCGATATGGGCGAACCGCGCTTGACAAAATCCG
AGCTTCCTATGCTTGACGGCGGGGAAGAGCACACAATCAATGAAACGATGGCATTTGGTGAAGTAGAACTAACAGGCACT
GCCGTTTCAATGGGAAATCCGCATATCGTTTTTCCGATCGCAGACATTGAACAGGCACCCTTAACGACACTGGGGCCGGT
GATTGAGAAAGATCCGAGATTTCCGGAAGGCATCAATGTGGAATTCGTTGAAACCGTGAATGAACAAGAATTGCATTTCC
GCGTCTGGGAACGGGGATCAGGGATTACTCAGGCATGCGGGACAGGAGCATGTGCAGCGGCAGTCGCATCTGTATTAAAC
GGCGTATCAAAGCGAAATCAAGACATCACGGTGCATTTAGCAGGCGGGGATTTGGTGATAAACTGGAAAGATAACGGCCA
TGTAATGATGACAGGGCCGGCTGAAACCGTTTGTGAGGGTGTCTATTTCTTGTAA

Upstream 100 bases:

>100_bases
GTGCAAGGAGCTTTTATGTATGAAAAACGGGGGATAATTGTATTGTGTCCGCTTATAATAGCTTTTATACTAAGAAAAAG
AAAAAAAGTTGGAGGCACCT

Downstream 100 bases:

>100_bases
AACACCTTGTTTGAGAATTGGAACGATCATGTTTATAATAAAAGACAGCAACCGGTTTTTAAAAGGAGGTTCATAATGAT
GAGCAACCCGGTAACAATTA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNSFRFTKMHGLGNSYIYVNQFEEQLPEEKLSEIAIQVSSVYTGIGSDGMILICPSDQAPVKMRIFNNDGSEGKNCGNGL
RCVAKYAYEHKLVEETSFLIETLSGLVKAEVQVENGKVNVVTVDMGEPRLTKSELPMLDGGEEHTINETMAFGEVELTGT
AVSMGNPHIVFPIADIEQAPLTTLGPVIEKDPRFPEGINVEFVETVNEQELHFRVWERGSGITQACGTGACAAAVASVLN
GVSKRNQDITVHLAGGDLVINWKDNGHVMMTGPAETVCEGVYFL

Sequences:

>Translated_284_residues
MNSFRFTKMHGLGNSYIYVNQFEEQLPEEKLSEIAIQVSSVYTGIGSDGMILICPSDQAPVKMRIFNNDGSEGKNCGNGL
RCVAKYAYEHKLVEETSFLIETLSGLVKAEVQVENGKVNVVTVDMGEPRLTKSELPMLDGGEEHTINETMAFGEVELTGT
AVSMGNPHIVFPIADIEQAPLTTLGPVIEKDPRFPEGINVEFVETVNEQELHFRVWERGSGITQACGTGACAAAVASVLN
GVSKRNQDITVHLAGGDLVINWKDNGHVMMTGPAETVCEGVYFL
>Mature_284_residues
MNSFRFTKMHGLGNSYIYVNQFEEQLPEEKLSEIAIQVSSVYTGIGSDGMILICPSDQAPVKMRIFNNDGSEGKNCGNGL
RCVAKYAYEHKLVEETSFLIETLSGLVKAEVQVENGKVNVVTVDMGEPRLTKSELPMLDGGEEHTINETMAFGEVELTGT
AVSMGNPHIVFPIADIEQAPLTTLGPVIEKDPRFPEGINVEFVETVNEQELHFRVWERGSGITQACGTGACAAAVASVLN
GVSKRNQDITVHLAGGDLVINWKDNGHVMMTGPAETVCEGVYFL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=283, Percent_Identity=36.3957597173145, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_BACSU (O32114)

Other databases:

- EMBL:   AL009126
- PIR:   F70024
- RefSeq:   NP_391097.1
- ProteinModelPortal:   O32114
- SMR:   O32114
- EnsemblBacteria:   EBBACT00000003628
- GeneID:   937110
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32170
- NMPDR:   fig|224308.1.peg.3223
- GenoList:   BSU32170
- GeneTree:   EBGT00050000002647
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   O32114
- ProtClustDB:   PRK00450
- BioCyc:   BSUB:BSU32170-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30871; Mature: 30871

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFRFTKMHGLGNSYIYVNQFEEQLPEEKLSEIAIQVSSVYTGIGSDGMILICPSDQAP
CCCCEEEEECCCCCCEEEEECHHHHCCHHHHHHHHEEHHHHEECCCCCCEEEEECCCCCC
VKMRIFNNDGSEGKNCGNGLRCVAKYAYEHKLVEETSFLIETLSGLVKAEVQVENGKVNV
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEE
VTVDMGEPRLTKSELPMLDGGEEHTINETMAFGEVELTGTAVSMGNPHIVFPIADIEQAP
EEEECCCCCCCHHHCCCCCCCCCCCCHHHEEECEEEEEEEEEECCCCEEEEEECCCCCCC
LTTLGPVIEKDPRFPEGINVEFVETVNEQELHFRVWERGSGITQACGTGACAAAVASVLN
CHHHCHHHCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHH
GVSKRNQDITVHLAGGDLVINWKDNGHVMMTGPAETVCEGVYFL
HHHHCCCEEEEEEECCEEEEEEECCCEEEEECCHHHHHCCCCCC
>Mature Secondary Structure
MNSFRFTKMHGLGNSYIYVNQFEEQLPEEKLSEIAIQVSSVYTGIGSDGMILICPSDQAP
CCCCEEEEECCCCCCEEEEECHHHHCCHHHHHHHHEEHHHHEECCCCCCEEEEECCCCCC
VKMRIFNNDGSEGKNCGNGLRCVAKYAYEHKLVEETSFLIETLSGLVKAEVQVENGKVNV
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEE
VTVDMGEPRLTKSELPMLDGGEEHTINETMAFGEVELTGTAVSMGNPHIVFPIADIEQAP
EEEECCCCCCCHHHCCCCCCCCCCCCHHHEEECEEEEEEEEEECCCCEEEEEECCCCCCC
LTTLGPVIEKDPRFPEGINVEFVETVNEQELHFRVWERGSGITQACGTGACAAAVASVLN
CHHHCHHHCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHH
GVSKRNQDITVHLAGGDLVINWKDNGHVMMTGPAETVCEGVYFL
HHHHCCCEEEEEEECCEEEEEEECCCEEEEECCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377