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The map label for this gene is dhbB

Identifier: 16080250

GI number: 16080250

Start: 3287675

End: 3288613

Strand: Reverse

Name: dhbB

Synonym: BSU31970

Alternate gene names: 16080250

Gene position: 3288613-3287675 (Counterclockwise)

Preceding gene: 16080251

Following gene: 255767732

Centisome position: 78.01

GC content: 46.54

Gene sequence:

>939_bases
ATGGCTATACCTGCCATTCAGCCGTATCAAATGCCGACAGCATCTGATATGCCGCAAAACAAAGTATCATGGGTGCCTGA
TCCGAATCGGGCTGTCTTGTTAATACACGATATGCAAAACTATTTTGTTGATGCTTTCACAGCGGGAGCGTCTCCGGTAA
CAGAGCTTTCAGCGAATATACGAAAGCTGAAGAATCAATGTGTTCAGCTTGGGATTCCTGTTGTCTATACCGCACAGCCG
GGAAGCCAAAATCCGGATGACCGTGCGCTGCTGACAGACTTTTGGGGCCCGGGATTAAACAGCGGTCCTTATGAGGAGAA
AATTATAACCGAGCTGGCACCAGAGGATGATGATCTTGTGCTGACAAAATGGAGATACAGCGCGTTTAAGAGAACGAATC
TGCTTGAAATGATGCGCAAAGAGGGACGCGATCAGCTGATCATTACAGGAATTTACGCCCATATCGGCTGTCTTGTTACA
GCATGTGAAGCATTTATGGAGGATATTAAAGCCTTTTTTGTGGGAGATGCAGTTGCTGATTTTTCATTAGAAAAACATCA
AATGGCGCTGGAATATGCGGCTGGACGCTGTGCGTTTACCGTGATGACTGACAGTCTTCTTGATCAGCTGCAGAATGCGC
CGGCAGACGTTCAAAAAACGTCAGCAAACACTGGCAAAAAGAACGTGTTTACATGTGAGAATATCCGTAAACAAATTGCT
GAGCTTCTACAAGAAACACCGGAAGACATCACAGATCAAGAGGATTTGCTCGATCGTGGTCTTGATTCGGTAAGGATCAT
GACATTGGTGGAACAATGGCGCCGTGAAGGGGCAGAGGTGACTTTCGTGGAATTGGCTGAACGCCCAACGATCGAAGAAT
GGCAGAAATTGCTCACAACTCGCAGCCAGCAAGTGCTGCCAAACGCGGATTATTTATAA

Upstream 100 bases:

>100_bases
AGTAGGAAAAGTCAGCAAAAAAGCGCTCCGTGAAGCCATTTCCGAGAAGCTTCTTGCAGGATTTAAAAAATAAAACAACA
ATTTGAGAGGAAGTGTTCAT

Downstream 100 bases:

>100_bases
AAGGAGGGTAAACAAACGGATGCCTGATACAAAAGATCTTCAATATTCTTTGACCGGGGCGCAAACTGGCATATGGTTTG
CTCAGCAGCTTGATCCGGAC

Product: isochorismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Superoxide-inducible protein 1; SOI1

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQP
GSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVT
ACEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA
ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLTTRSQQVLPNADYL

Sequences:

>Translated_312_residues
MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQP
GSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVT
ACEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA
ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLTTRSQQVLPNADYL
>Mature_311_residues
AIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQPG
SQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTA
CEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIAE
LLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLTTRSQQVLPNADYL

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain

Homologues:

Organism=Escherichia coli, GI1786811, Length=299, Percent_Identity=48.8294314381271, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHBB_BACSU (P45743)

Other databases:

- EMBL:   U26444
- EMBL:   AL009126
- PIR:   B69615
- RefSeq:   NP_391077.1
- ProteinModelPortal:   P45743
- SMR:   P45743
- EnsemblBacteria:   EBBACT00000001616
- GeneID:   936582
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31970
- NMPDR:   fig|224308.1.peg.3203
- GenoList:   BSU31970
- GeneTree:   EBGT00050000000753
- HOGENOM:   HBG289118
- OMA:   YASNRCA
- PhylomeDB:   P45743
- ProtClustDB:   CLSK887817
- BioCyc:   BSUB:BSU31970-MONOMER
- BioCyc:   MetaCyc:MONOMER-13911
- BRENDA:   3.3.2.1
- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163
- Gene3D:   G3DSA:1.10.1200.10
- Gene3D:   G3DSA:3.40.50.850
- PIRSF:   PIRSF001111
- PRINTS:   PR01398

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding; SSF47336 ACP_like; SSF52499 Iscrsm_hydrolase

EC number: =3.3.2.1

Molecular weight: Translated: 35107; Mature: 34976

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50075 ACP_DOMAIN; PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCHHHHHHHHH
RKLKNQCVQLGIPVVYTAQPGSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLV
HHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEE
LTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVAD
EEEHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA
HHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEHHHHHHHHH
ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLTT
HHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHHH
RSQQVLPNADYL
HHHHCCCCCCCC
>Mature Secondary Structure 
AIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCHHHHHHHHH
RKLKNQCVQLGIPVVYTAQPGSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLV
HHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEE
LTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVAD
EEEHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA
HHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEHHHHHHHHH
ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLTT
HHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHHH
RSQQVLPNADYL
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550523; 9384377; 9298659