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The map label for this gene is lytG

Identifier: 16080164

GI number: 16080164

Start: 3190834

End: 3191682

Strand: Reverse

Name: lytG

Synonym: BSU31120

Alternate gene names: 16080164

Gene position: 3191682-3190834 (Counterclockwise)

Preceding gene: 16080165

Following gene: 16080163

Centisome position: 75.71

GC content: 41.7

Gene sequence:

>849_bases
ATGGCCCGTAAAAAACTTAAAAAACGTAAACTCTTGATTTCACTATTTTTCCTTGTATCGATTCCGCTAGCTCTGTTTGT
TTTAGCGACAACTTTATCAAAACCGATTGAAATATCCAAAGAAACTGAAGAAATCGATGAACAGCAAGTATTTATAGACA
GTCTGTCCGGACATGCCCAAATTTTGTATGAAAAATACCATGTTCTCCCCAGCATCACAATTGCTCAGGCCATTCTCGAA
TCGGACTGGGGGAACAGTGAACTGGCTGCTAAGGCCAACAACCTTTTTGGCGTAAAAGGGAATTATAAAGGCCATCATGT
CACGATGGAAACGGACGAAGTTGAAAAAGGGAAAAGAAAAACCATTCGCGCGAAGTTTCGAAAATACAGCACGTTTTTTG
AATCTATGGATGATCACGCCCAATTGTTCGTCCGTGGCACATCGTGGAATAAAAAGAAGTATAAACCGGTGCTTGAGGCA
GGGAATTATAAGGAAGCGGCAACCGCCCTGCAAACATCAGGATATGCTACAGACCCTGACTACGCTGACAAAATCAGCGC
TATTGTGGAAAAATACGATTTAGATGAGTACGATGAGGTAAATCCATCCCTCAAATCTGTGGATTTAAACGCCTCTATTA
AAGACAGTGCCGTTCAAGACGTATGGTCCAAGCCTTCTACTGATGATCGGTCCATAAGGCTTACCTCTGCCCAATCTTAC
GTCGGCAAAGACATAAAGGTTGTGTCTAAAAAACAGAAAGGCCAGTCTGTATGGTACCAATTCCAAATCAATGATAAACT
TATCGGCTGGATTGATGATTCAGCTGTTGAAATAAAGGAGGCAACCTAA

Upstream 100 bases:

>100_bases
CTTTATCCCTAAAGAATTGAAAAATATTAGAAGTGCAAGTAAAATTCTAAAAGGTAGATTCTTTTACATTCGTAATCTTT
TAATTCGAAGGAAGGTTTTC

Downstream 100 bases:

>100_bases
GCCCTTTTTACTGGTATTTTTAATTCTTTGTTCCATTCCGGGGAATTTGAGGTATAATATTCCACGGAAGTTATCACTTT
TTAACTATGCTTTCGGGAGG

Product: exoglucosaminidase

Products: NA

Alternate protein names: Autolysin lytG; Exo-beta-N-acetylglucosaminidase lytG; Peptidoglycan hydrolase lytG

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MARKKLKKRKLLISLFFLVSIPLALFVLATTLSKPIEISKETEEIDEQQVFIDSLSGHAQILYEKYHVLPSITIAQAILE
SDWGNSELAAKANNLFGVKGNYKGHHVTMETDEVEKGKRKTIRAKFRKYSTFFESMDDHAQLFVRGTSWNKKKYKPVLEA
GNYKEAATALQTSGYATDPDYADKISAIVEKYDLDEYDEVNPSLKSVDLNASIKDSAVQDVWSKPSTDDRSIRLTSAQSY
VGKDIKVVSKKQKGQSVWYQFQINDKLIGWIDDSAVEIKEAT

Sequences:

>Translated_282_residues
MARKKLKKRKLLISLFFLVSIPLALFVLATTLSKPIEISKETEEIDEQQVFIDSLSGHAQILYEKYHVLPSITIAQAILE
SDWGNSELAAKANNLFGVKGNYKGHHVTMETDEVEKGKRKTIRAKFRKYSTFFESMDDHAQLFVRGTSWNKKKYKPVLEA
GNYKEAATALQTSGYATDPDYADKISAIVEKYDLDEYDEVNPSLKSVDLNASIKDSAVQDVWSKPSTDDRSIRLTSAQSY
VGKDIKVVSKKQKGQSVWYQFQINDKLIGWIDDSAVEIKEAT
>Mature_281_residues
ARKKLKKRKLLISLFFLVSIPLALFVLATTLSKPIEISKETEEIDEQQVFIDSLSGHAQILYEKYHVLPSITIAQAILES
DWGNSELAAKANNLFGVKGNYKGHHVTMETDEVEKGKRKTIRAKFRKYSTFFESMDDHAQLFVRGTSWNKKKYKPVLEAG
NYKEAATALQTSGYATDPDYADKISAIVEKYDLDEYDEVNPSLKSVDLNASIKDSAVQDVWSKPSTDDRSIRLTSAQSYV
GKDIKVVSKKQKGQSVWYQFQINDKLIGWIDDSAVEIKEAT

Specific function: Is the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Acts processively from the ends of the glycan strands. Also plays a role in motility, chemotaxis and cell division

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Secreted, cell wall

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 73 family

Homologues:

Organism=Escherichia coli, GI1787321, Length=149, Percent_Identity=36.9127516778523, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): LYTG_BACSU (O32083)

Other databases:

- EMBL:   AL009126
- PIR:   H70006
- RefSeq:   NP_390990.1
- ProteinModelPortal:   O32083
- SMR:   O32083
- EnsemblBacteria:   EBBACT00000002401
- GeneID:   937146
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31120
- NMPDR:   fig|224308.1.peg.3115
- GenoList:   BSU31120
- GeneTree:   EBGT00050000000238
- HOGENOM:   HBG726129
- OMA:   WGNSELA
- ProtClustDB:   CLSK887781
- BioCyc:   BSUB:BSU31120-MONOMER
- InterPro:   IPR000423
- InterPro:   IPR013338
- InterPro:   IPR002901
- PRINTS:   PR01002
- SMART:   SM00047

Pfam domain/function: PF01832 Glucosaminidase

EC number: 3.2.1.- [C]

Molecular weight: Translated: 31901; Mature: 31770

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKLKKRKLLISLFFLVSIPLALFVLATTLSKPIEISKETEEIDEQQVFIDSLSGHAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHH
ILYEKYHVLPSITIAQAILESDWGNSELAAKANNLFGVKGNYKGHHVTMETDEVEKGKRK
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCEEEECCCCCCCEEEECCHHHHHHHHH
TIRAKFRKYSTFFESMDDHAQLFVRGTSWNKKKYKPVLEAGNYKEAATALQTSGYATDPD
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHCCHHHHCCCCHHHHHHHHCCCCCCCCC
YADKISAIVEKYDLDEYDEVNPSLKSVDLNASIKDSAVQDVWSKPSTDDRSIRLTSAQSY
HHHHHHHHHHHHCCCCHHHCCCCHHEEECCCCHHHHHHHHHHCCCCCCCCEEEEECHHHH
VGKDIKVVSKKQKGQSVWYQFQINDKLIGWIDDSAVEIKEAT
HCCCHHHHHHHCCCCEEEEEEEECCEEEEEECCCCEEEECCC
>Mature Secondary Structure 
ARKKLKKRKLLISLFFLVSIPLALFVLATTLSKPIEISKETEEIDEQQVFIDSLSGHAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHH
ILYEKYHVLPSITIAQAILESDWGNSELAAKANNLFGVKGNYKGHHVTMETDEVEKGKRK
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCEEEECCCCCCCEEEECCHHHHHHHHH
TIRAKFRKYSTFFESMDDHAQLFVRGTSWNKKKYKPVLEAGNYKEAATALQTSGYATDPD
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHCCHHHHCCCCHHHHHHHHCCCCCCCCC
YADKISAIVEKYDLDEYDEVNPSLKSVDLNASIKDSAVQDVWSKPSTDDRSIRLTSAQSY
HHHHHHHHHHHHCCCCHHHCCCCHHEEECCCCHHHHHHHHHHCCCCCCCCEEEEECHHHH
VGKDIKVVSKKQKGQSVWYQFQINDKLIGWIDDSAVEIKEAT
HCCCHHHHHHHCCCCEEEEEEEECCEEEEEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377