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The map label for this gene is glgB

Identifier: 16080150

GI number: 16080150

Start: 3169763

End: 3171646

Strand: Reverse

Name: glgB

Synonym: BSU30980

Alternate gene names: 16080150

Gene position: 3171646-3169763 (Counterclockwise)

Preceding gene: 16080152

Following gene: 16080149

Centisome position: 75.24

GC content: 42.2

Gene sequence:

>1884_bases
ATGGCCGCTGCCAGCCCGACAGCACATGATGTTTATCTTTTTCACGAAGGCAGTCTGTTTAAAAGCTATCAGCTGTTTGG
CTCGCATTACCGCGAGCTGAATGGCAAAAGCGGATATGAATTCTGTGTGTGGGCGCCTCATGCGTCGGAAGTACGAGTGG
CTGGAGATTTTAACAGCTGGTCTGGAGAAGAGCATGTGATGCACAGAGTGAATGATAATGGCATTTGGACGTTATTTATT
CCAGGTATAGGAGAAAAGGAACGATATAAATACGAAATTGTGACGAATAACGGTGAGATCAGGCTGAAGGCAGATCCATA
CGCTATTTATTCAGAAGTCAGACCAAACACAGCGTCACTTACTTACGATTTGGAAGGATACAGCTGGCAAGATCAAAAAT
GGCAAAAGAAACAGAAGGCTAAGACGCTGTATGAAAAACCTGTTTTCATTTATGAACTGCATCTTGGGTCTTGGAAGAAG
CATTCTGATGGCAGGCATTATTCCTATAAAGAATTAAGTCAGACGCTTATCCCTTATATCAAGAAGCATGGGTTTACCCA
TATTGAACTGCTTCCCGTGTATGAGCATCCATATGATCGTTCATGGGGGTACCAAGGAACGGGGTATTACAGCCCGACAA
GCAGGTTTGGCCCGCCGCATGATCTGATGAAGTTTGTGGATGAATGTCATCAACAAAATATCGGAGTCATTTTGGATTGG
GTCCCCGGACATTTTTGTAAAGATGCACATGGCCTTTATATGTTTGATGGAGAACCGCTTTATGAATACAAAGAAGAACG
TGATCGGGAGAACTGGTTATGGGGCACGGCAAACTTTGACCTGGGGAAACCGGAAGTTCATAGTTTTTTAATTTCGAATG
CGTTGTACTGGGCGGAATTCTATCATATAGATGGATTTAGGGTTGATGCAGTGGCTAATATCTTGTATTGGCCAAATCAG
GATGAACGCCACACGAATCCATATGCTGTTGATTTTTTAAAAAAACTCAATCAAACCATGAGAGAGGCATATCCCCATGT
GATGATGATTGCAGAAGATTCTACAGAATGGCCTCAAGTGACAGGTGCCGTTGAGGAAGGGGGGCTTGGTTTTCATTATA
AATGGAATATGGGATGGATGAATGACGTACTGAAATACATGGAGACGCCGCCCGAAGAAAGGCGGCATTGTCATCAGCTT
ATCTCTTTTTCATTACTATATGCGTTTAGTGAACATTTTGTATTGCCGTTTTCTCATGATGAAGTCGTATACGGAAAAAA
ATCACTTCTCAATAAAATGCCTGGCGACTACTGGCAGAAATTTGCCCAGTATCGGCTTTTGCTGGGCTATATGACAGTGC
ATCCCGGAAAGAAATTGATCTTTATGGGATCAGAGTTTGCTCAATTTGATGAATGGAAAGATACAGAACAGCTTGATTGG
TTTTTGGACTCCTTCCCCATGCATCAGAAAGCCAGCGTTTTTACGCAAGATCTTCTCCGCTTTTACCAAAAAAGCAAAAT
CCTTTATGAGCATGACCATCGTGCACAATCGTTTGAATGGATTGATGTCCATAATGACGAACAATCAATCTTTTCTTTTA
TTCGTTATGGCCAAAAGCACGGTGAGGCGCTTGTCATCATTTGTAATTTTACACCCGTGGTCTATCATCAATATGATGTT
GGAGTTCCTTTTTTTACTCAATACATTGAAGTGCTTAACAGTGACAGTGAAACATATGGAGGCTCAGGGCAAATCAACAA
AAAGCCGTTATCGGCTAAAAAGGGAGCACTTCATCATAAACCGTGTTATATCACGATGACCATCCCCCCTTACGGCATCT
CGATTTTACGGGCGGTTAAAAAAAGAGGAGAGATAAAAAGATGA

Upstream 100 bases:

>100_bases
TAATTGTCTATAAGTATAAGCGCTTTCAGGAAAGGGCTTTTTTTTATTTCTTCGAATAAATACTATAAATGAAAACTATG
ATGTCAGAAAGGATGATTAC

Downstream 100 bases:

>100_bases
AAAAACAATGTGTAGCCATGCTCCTTGCCGGCGGGAAGGGCAGCCGTCTCAGCGGATTAACAAAAAATATGGCCAAACCG
GCTGTATCTTTCGGGGGAAA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MAAASPTAHDVYLFHEGSLFKSYQLFGSHYRELNGKSGYEFCVWAPHASEVRVAGDFNSWSGEEHVMHRVNDNGIWTLFI
PGIGEKERYKYEIVTNNGEIRLKADPYAIYSEVRPNTASLTYDLEGYSWQDQKWQKKQKAKTLYEKPVFIYELHLGSWKK
HSDGRHYSYKELSQTLIPYIKKHGFTHIELLPVYEHPYDRSWGYQGTGYYSPTSRFGPPHDLMKFVDECHQQNIGVILDW
VPGHFCKDAHGLYMFDGEPLYEYKEERDRENWLWGTANFDLGKPEVHSFLISNALYWAEFYHIDGFRVDAVANILYWPNQ
DERHTNPYAVDFLKKLNQTMREAYPHVMMIAEDSTEWPQVTGAVEEGGLGFHYKWNMGWMNDVLKYMETPPEERRHCHQL
ISFSLLYAFSEHFVLPFSHDEVVYGKKSLLNKMPGDYWQKFAQYRLLLGYMTVHPGKKLIFMGSEFAQFDEWKDTEQLDW
FLDSFPMHQKASVFTQDLLRFYQKSKILYEHDHRAQSFEWIDVHNDEQSIFSFIRYGQKHGEALVIICNFTPVVYHQYDV
GVPFFTQYIEVLNSDSETYGGSGQINKKPLSAKKGALHHKPCYITMTIPPYGISILRAVKKRGEIKR

Sequences:

>Translated_627_residues
MAAASPTAHDVYLFHEGSLFKSYQLFGSHYRELNGKSGYEFCVWAPHASEVRVAGDFNSWSGEEHVMHRVNDNGIWTLFI
PGIGEKERYKYEIVTNNGEIRLKADPYAIYSEVRPNTASLTYDLEGYSWQDQKWQKKQKAKTLYEKPVFIYELHLGSWKK
HSDGRHYSYKELSQTLIPYIKKHGFTHIELLPVYEHPYDRSWGYQGTGYYSPTSRFGPPHDLMKFVDECHQQNIGVILDW
VPGHFCKDAHGLYMFDGEPLYEYKEERDRENWLWGTANFDLGKPEVHSFLISNALYWAEFYHIDGFRVDAVANILYWPNQ
DERHTNPYAVDFLKKLNQTMREAYPHVMMIAEDSTEWPQVTGAVEEGGLGFHYKWNMGWMNDVLKYMETPPEERRHCHQL
ISFSLLYAFSEHFVLPFSHDEVVYGKKSLLNKMPGDYWQKFAQYRLLLGYMTVHPGKKLIFMGSEFAQFDEWKDTEQLDW
FLDSFPMHQKASVFTQDLLRFYQKSKILYEHDHRAQSFEWIDVHNDEQSIFSFIRYGQKHGEALVIICNFTPVVYHQYDV
GVPFFTQYIEVLNSDSETYGGSGQINKKPLSAKKGALHHKPCYITMTIPPYGISILRAVKKRGEIKR
>Mature_626_residues
AAASPTAHDVYLFHEGSLFKSYQLFGSHYRELNGKSGYEFCVWAPHASEVRVAGDFNSWSGEEHVMHRVNDNGIWTLFIP
GIGEKERYKYEIVTNNGEIRLKADPYAIYSEVRPNTASLTYDLEGYSWQDQKWQKKQKAKTLYEKPVFIYELHLGSWKKH
SDGRHYSYKELSQTLIPYIKKHGFTHIELLPVYEHPYDRSWGYQGTGYYSPTSRFGPPHDLMKFVDECHQQNIGVILDWV
PGHFCKDAHGLYMFDGEPLYEYKEERDRENWLWGTANFDLGKPEVHSFLISNALYWAEFYHIDGFRVDAVANILYWPNQD
ERHTNPYAVDFLKKLNQTMREAYPHVMMIAEDSTEWPQVTGAVEEGGLGFHYKWNMGWMNDVLKYMETPPEERRHCHQLI
SFSLLYAFSEHFVLPFSHDEVVYGKKSLLNKMPGDYWQKFAQYRLLLGYMTVHPGKKLIFMGSEFAQFDEWKDTEQLDWF
LDSFPMHQKASVFTQDLLRFYQKSKILYEHDHRAQSFEWIDVHNDEQSIFSFIRYGQKHGEALVIICNFTPVVYHQYDVG
VPFFTQYIEVLNSDSETYGGSGQINKKPLSAKKGALHHKPCYITMTIPPYGISILRAVKKRGEIKR

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=653, Percent_Identity=26.1868300153139, Blast_Score=171, Evalue=3e-42,
Organism=Escherichia coli, GI1789839, Length=616, Percent_Identity=43.1818181818182, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17554896, Length=615, Percent_Identity=26.3414634146341, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32564391, Length=534, Percent_Identity=26.0299625468165, Blast_Score=148, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6320826, Length=628, Percent_Identity=26.7515923566879, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI28573410, Length=616, Percent_Identity=26.461038961039, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_BACSU (P39118)

Other databases:

- EMBL:   Z25795
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   S40048
- RefSeq:   NP_390976.1
- ProteinModelPortal:   P39118
- SMR:   P39118
- EnsemblBacteria:   EBBACT00000003503
- GeneID:   937993
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30980
- NMPDR:   fig|224308.1.peg.3101
- GenoList:   BSU30980
- GeneTree:   EBGT00070000031950
- HOGENOM:   HBG287139
- OMA:   RMQASAI
- PhylomeDB:   P39118
- ProtClustDB:   PRK05402
- BioCyc:   BSUB:BSU30980-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 73666; Mature: 73535

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: ACT_SITE 204-204 ACT_SITE 239-239 ACT_SITE 244-244 ACT_SITE 307-307 ACT_SITE 309-309 ACT_SITE 352-352 ACT_SITE 419-419 ACT_SITE 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAASPTAHDVYLFHEGSLFKSYQLFGSHYRELNGKSGYEFCVWAPHASEVRVAGDFNSW
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCC
SGEEHVMHRVNDNGIWTLFIPGIGEKERYKYEIVTNNGEIRLKADPYAIYSEVRPNTASL
CCCHHHHHEECCCCEEEEEECCCCCCCCEEEEEEECCCEEEEEECCEEEEHHCCCCCEEE
TYDLEGYSWQDQKWQKKQKAKTLYEKPVFIYELHLGSWKKHSDGRHYSYKELSQTLIPYI
EEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
KKHGFTHIELLPVYEHPYDRSWGYQGTGYYSPTSRFGPPHDLMKFVDECHQQNIGVILDW
HHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
VPGHFCKDAHGLYMFDGEPLYEYKEERDRENWLWGTANFDLGKPEVHSFLISNALYWAEF
CCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEE
YHIDGFRVDAVANILYWPNQDERHTNPYAVDFLKKLNQTMREAYPHVMMIAEDSTEWPQV
EEECCEEHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCE
TGAVEEGGLGFHYKWNMGWMNDVLKYMETPPEERRHCHQLISFSLLYAFSEHFVLPFSHD
ECCHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCC
EVVYGKKSLLNKMPGDYWQKFAQYRLLLGYMTVHPGKKLIFMGSEFAQFDEWKDTEQLDW
CEEECHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHCCCCCHHHHHH
FLDSFPMHQKASVFTQDLLRFYQKSKILYEHDHRAQSFEWIDVHNDEQSIFSFIRYGQKH
HHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHCC
GEALVIICNFTPVVYHQYDVGVPFFTQYIEVLNSDSETYGGSGQINKKPLSAKKGALHHK
CCEEEEEEECCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PCYITMTIPPYGISILRAVKKRGEIKR
CEEEEEEECCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AAASPTAHDVYLFHEGSLFKSYQLFGSHYRELNGKSGYEFCVWAPHASEVRVAGDFNSW
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCC
SGEEHVMHRVNDNGIWTLFIPGIGEKERYKYEIVTNNGEIRLKADPYAIYSEVRPNTASL
CCCHHHHHEECCCCEEEEEECCCCCCCCEEEEEEECCCEEEEEECCEEEEHHCCCCCEEE
TYDLEGYSWQDQKWQKKQKAKTLYEKPVFIYELHLGSWKKHSDGRHYSYKELSQTLIPYI
EEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
KKHGFTHIELLPVYEHPYDRSWGYQGTGYYSPTSRFGPPHDLMKFVDECHQQNIGVILDW
HHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
VPGHFCKDAHGLYMFDGEPLYEYKEERDRENWLWGTANFDLGKPEVHSFLISNALYWAEF
CCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEE
YHIDGFRVDAVANILYWPNQDERHTNPYAVDFLKKLNQTMREAYPHVMMIAEDSTEWPQV
EEECCEEHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCE
TGAVEEGGLGFHYKWNMGWMNDVLKYMETPPEERRHCHQLISFSLLYAFSEHFVLPFSHD
ECCHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCC
EVVYGKKSLLNKMPGDYWQKFAQYRLLLGYMTVHPGKKLIFMGSEFAQFDEWKDTEQLDW
CEEECHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHCCCCCHHHHHH
FLDSFPMHQKASVFTQDLLRFYQKSKILYEHDHRAQSFEWIDVHNDEQSIFSFIRYGQKH
HHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHCC
GEALVIICNFTPVVYHQYDVGVPFFTQYIEVLNSDSETYGGSGQINKKPLSAKKGALHHK
CCEEEEEEECCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PCYITMTIPPYGISILRAVKKRGEIKR
CEEEEEEECCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8145641; 9387221; 9384377