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The map label for this gene is cotS

Identifier: 16080142

GI number: 16080142

Start: 3159691

End: 3160746

Strand: Reverse

Name: cotS

Synonym: BSU30900

Alternate gene names: 16080142

Gene position: 3160746-3159691 (Counterclockwise)

Preceding gene: 16080143

Following gene: 16080141

Centisome position: 74.98

GC content: 43.09

Gene sequence:

>1056_bases
GTGTACCAAAAAGAGCATGAAGAACAGATTGTGTCCGAAATTCTCAGTTATTATCCGTTTCATATCGACCATGTGGCGCT
GAAATCGAACAAAAGCGGGCGCAAAATCTGGGAAGTCGAAACTGATCATGGCCCAAAGCTGCTAAAAGAAGCGCAAATGA
AACCGGAGCGGATGCTTTTTATCACTCAGGCACACGCCCATTTACAAGAGAAAGGGCTGCCGATAGCGCCGATTCATCAA
ACAAAAAATGGCGGTAGCTGCTTGGGCACGGATCAGGTTTCTTACAGTTTATATGACAAAGTGACAGGAAAAGAAATGAT
TTACTATGATGCAGAGCAAATGAAAAAAGTCATGTCATTTGCCGGCCATTTTCATCATGCCTCAAAAGGATATGTTTGCA
CAGATGAAAGCAAGAAGAGAAGCAGGCTGGGAAAATGGCACAAATTGTACCGTTGGAAGCTGCAGGAACTTGAAGGGAAT
ATGCAGATCGCAGCATCCTATCCTGATGACGTATTTTCGCAAACTTTCTTAAAACATGCTGATAAAATGCTGGCAAGAGG
AAAAGAAGCTCTCAGAGCGCTTGATGACTCAGAATACGAAACCTGGACAAAAGAGACACTCGAGCATGGCGGATTCTGTT
TTCAGGATTTTACATTGGCACGTTTGACTGAGATCGAAGGGGAGCCTTTTTTAAAGGAGCTTCACTCGATTACCTACGAT
TTGCCGTCAAGAGACCTTCGTATTCTGCTGAATAAAGTGATGGTTAAGCTTTCTGTATGGGATACTGATTTCATGGTTGC
ACTGCTTGCGGCCTACGACGCAGTGTATCCGCTCACAGAAAAACAGTACGAGGTACTTTGGATTGATCTCGCGTTTCCGC
ATTTGTTCTGTGCAATCGGGCACAAATATTATTTGAAGCAAAAGAAAACGTGGTCAGATGAGAAGTATAACTGGGCGCTG
CAAAACATGATTTCTGTTGAAGAATCTAAAGATTCGTTTTTGGATAAACTGCCGGAACTGTATAAAAAGATAAAAGCGTA
TCGGGAGGCGAATTGA

Upstream 100 bases:

>100_bases
AATACAGCCGCCGTGAGGCAGAAAGCAATTTTGGCTGGCAGAGGGTGGCTGAAAATCTGCTCAGCGTCTATGAAAAGAAC
AGATAGAAGGGGTGAACAGA

Downstream 100 bases:

>100_bases
CATGAGGTCATCGAGACAAAAAGCCTCTATTTCTAAACTGGATCTCGACCAATTCGTATTTACACCGCCTGGACCGATGG
GATGGCAGGCTCATGACTCA

Product: spore coat protein

Products: NA

Alternate protein names: Coat protein 40 kDa component 2; COT40-2

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MYQKEHEEQIVSEILSYYPFHIDHVALKSNKSGRKIWEVETDHGPKLLKEAQMKPERMLFITQAHAHLQEKGLPIAPIHQ
TKNGGSCLGTDQVSYSLYDKVTGKEMIYYDAEQMKKVMSFAGHFHHASKGYVCTDESKKRSRLGKWHKLYRWKLQELEGN
MQIAASYPDDVFSQTFLKHADKMLARGKEALRALDDSEYETWTKETLEHGGFCFQDFTLARLTEIEGEPFLKELHSITYD
LPSRDLRILLNKVMVKLSVWDTDFMVALLAAYDAVYPLTEKQYEVLWIDLAFPHLFCAIGHKYYLKQKKTWSDEKYNWAL
QNMISVEESKDSFLDKLPELYKKIKAYREAN

Sequences:

>Translated_351_residues
MYQKEHEEQIVSEILSYYPFHIDHVALKSNKSGRKIWEVETDHGPKLLKEAQMKPERMLFITQAHAHLQEKGLPIAPIHQ
TKNGGSCLGTDQVSYSLYDKVTGKEMIYYDAEQMKKVMSFAGHFHHASKGYVCTDESKKRSRLGKWHKLYRWKLQELEGN
MQIAASYPDDVFSQTFLKHADKMLARGKEALRALDDSEYETWTKETLEHGGFCFQDFTLARLTEIEGEPFLKELHSITYD
LPSRDLRILLNKVMVKLSVWDTDFMVALLAAYDAVYPLTEKQYEVLWIDLAFPHLFCAIGHKYYLKQKKTWSDEKYNWAL
QNMISVEESKDSFLDKLPELYKKIKAYREAN
>Mature_351_residues
MYQKEHEEQIVSEILSYYPFHIDHVALKSNKSGRKIWEVETDHGPKLLKEAQMKPERMLFITQAHAHLQEKGLPIAPIHQ
TKNGGSCLGTDQVSYSLYDKVTGKEMIYYDAEQMKKVMSFAGHFHHASKGYVCTDESKKRSRLGKWHKLYRWKLQELEGN
MQIAASYPDDVFSQTFLKHADKMLARGKEALRALDDSEYETWTKETLEHGGFCFQDFTLARLTEIEGEPFLKELHSITYD
LPSRDLRILLNKVMVKLSVWDTDFMVALLAAYDAVYPLTEKQYEVLWIDLAFPHLFCAIGHKYYLKQKKTWSDEKYNWAL
QNMISVEESKDSFLDKLPELYKKIKAYREAN

Specific function: Seems to be required for the assembly of the CotSA protein in spores

COG id: NA

COG function: NA

Gene ontology:

Cell location: Spore wall, spore coat

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CotS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COTS_BACSU (P46914)

Other databases:

- EMBL:   D31847
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   I39808
- RefSeq:   NP_390968.1
- ProteinModelPortal:   P46914
- SMR:   P46914
- EnsemblBacteria:   EBBACT00000001419
- GeneID:   936628
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30900
- NMPDR:   fig|224308.1.peg.3093
- GenoList:   BSU30900
- GeneTree:   EBGT00050000002703
- HOGENOM:   HBG343332
- OMA:   PHLFCAI
- ProtClustDB:   CLSK887769
- BioCyc:   BSUB:BSU30900-MONOMER
- InterPro:   IPR014255
- TIGRFAMs:   TIGR02906

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 41084; Mature: 41084

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQKEHEEQIVSEILSYYPFHIDHVALKSNKSGRKIWEVETDHGPKLLKEAQMKPERMLF
CCCHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCEEEEEECCCCHHHHHHHHCCCHHEEE
ITQAHAHLQEKGLPIAPIHQTKNGGSCLGTDQVSYSLYDKVTGKEMIYYDAEQMKKVMSF
EHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHH
AGHFHHASKGYVCTDESKKRSRLGKWHKLYRWKLQELEGNMQIAASYPDDVFSQTFLKHA
HHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
DKMLARGKEALRALDDSEYETWTKETLEHGGFCFQDFTLARLTEIEGEPFLKELHSITYD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCC
LPSRDLRILLNKVMVKLSVWDTDFMVALLAAYDAVYPLTEKQYEVLWIDLAFPHLFCAIG
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHC
HKYYLKQKKTWSDEKYNWALQNMISVEESKDSFLDKLPELYKKIKAYREAN
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYQKEHEEQIVSEILSYYPFHIDHVALKSNKSGRKIWEVETDHGPKLLKEAQMKPERMLF
CCCHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCEEEEEECCCCHHHHHHHHCCCHHEEE
ITQAHAHLQEKGLPIAPIHQTKNGGSCLGTDQVSYSLYDKVTGKEMIYYDAEQMKKVMSF
EHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHH
AGHFHHASKGYVCTDESKKRSRLGKWHKLYRWKLQELEGNMQIAASYPDDVFSQTFLKHA
HHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
DKMLARGKEALRALDDSEYETWTKETLEHGGFCFQDFTLARLTEIEGEPFLKELHSITYD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCC
LPSRDLRILLNKVMVKLSVWDTDFMVALLAAYDAVYPLTEKQYEVLWIDLAFPHLFCAIG
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHC
HKYYLKQKKTWSDEKYNWALQNMISVEESKDSFLDKLPELYKKIKAYREAN
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545510; 9387221; 9384377; 8289687