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The map label for this gene is menD

Identifier: 16080134

GI number: 16080134

Start: 3150563

End: 3152305

Strand: Reverse

Name: menD

Synonym: BSU30820

Alternate gene names: 16080134

Gene position: 3152305-3150563 (Counterclockwise)

Preceding gene: 16080135

Following gene: 16080133

Centisome position: 74.78

GC content: 48.94

Gene sequence:

>1743_bases
TTGACAGTCAACCCGATTACTCATTACATCGGAAGTTTTATTGATGAATTCGCCCTTTCCGGAATTACGGACGCCGTCGT
TTGTCCGGGCTCAAGATCTACACCGCTGGCTGTTCTTTGTGCTGCGCATCCCGATATCAGCGTCCATGTCCAAATTGATG
AAAGGTCTGCCGGATTTTTTGCATTAGGTTTGGCAAAAGCGAAGCAGCGTCCCGTACTATTGATCTGTACATCTGGAACA
GCCGCGGCCAACTTTTATCCGGCTGTGGTTGAAGCCCATTATTCAAGGGTGCCTATTATTGTGTTAACTGCTGATCGGCC
TCATGAGCTGCGCGAAGTAGGCGCACCGCAGGCTATTAATCAGCACTTCTTATTCGGTAACTTTGTTAAGTTTTTCACAG
ACTCTGCTCTTCCTGAAGAGTCTCCGCAAATGCTGAGATACATCCGAACGCTGGCAAGCCGCGCTGCCGGAGAAGCACAA
AAGCGCCCGATGGGGCCTGTCCATGTGAATGTGCCGCTGCGCGAACCGCTGATGCCGGATCTTTCAGATGAGCCGTTTGG
AAGAATGAGAACAGGCCGTCATGTGTCTGTGAAAACGGGTACGCAGTCTGTTGACCGAGAGTCCCTGTCTGATGTGGCTG
AGATGCTCGCAGAGGCTGAAAAAGGAATGATCGTCTGCGGAGAGCTCCATAGTGATGCGGACAAGGAAAACATTATTGCG
CTGTCAAAAGCGCTTCAGTATCCAATTTTAGCAGATCCGCTGTCTAATCTGCGGAATGGTGTTCATGATAAAAGCACTGT
TATTGATGCGTATGATTCGTTTTTAAAAGATGATGAGCTGAAGCGGAAACTCCGTCCTGATGTTGTCATTCGGTTTGGAC
CGATGCCTGTTTCAAAACCGGTTTTCTTATGGCTTAAGGATGATCCGACGATTCAGCAAATCGTGATTGACGAGGATGGA
GGGTGGAGAGACCCGACACAGGCAAGCGCGCATATGATTCATTGTAATGCGTCTGTTTTTGCTGAGGAGATCATGGCAGG
CCTTACTGCTGCCACCAGATCCTCAGAGTGGCTGGAAAAGTGGCAGTTTGTCAATGGGCGGTTCCGCGAGCATCTGCAAA
CAATCAGCAGTGAAGATGTTTCGTTTGAAGGCAATCTCTATCGAATCCTTCAGCATCTTGTGCCTGAAAACAGCTCGCTG
TTTGTGGGAAACAGCATGCCGATCAGGGATGTTGATACGTTTTTTGAAAAGCAGGACCGCCCTTTCCGGATTTATTCAAA
CCGGGGAGCAAACGGGATAGACGGTGTCGTCTCCTCTGCCATGGGGGTATGTGAAGGAACAAAAGCGCCTGTTACCCTTG
TGATTGGAGATTTATCTTTTTATCATGATTTAAACGGCCTGCTGGCGGCCAAAAAGCTGGGTATTCCTCTTACTGTGATT
CTTGTCAATAATGACGGAGGAGGGATTTTCTCCTTTTTGCCGCAGGCTTCTGAGAAGACACACTTTGAAGATTTGTTCGG
CACACCGACAGGGCTCGATTTCAAGCATGCGGCTGCACTTTACGGCGGAACGTATTCATGCCCGGCTTCTTGGGATGAAT
TCAAAACAGCTTACGCGCCGCAGGCAGACAAGCCCGGACTCCATTTGATTGAAATCAAAACGGATCGCCAATCAAGAGTC
CAGCTTCATCGCGATATGCTGAATGAGGCTGTGCGGGAAGTGAAAAAACAATGGGAACTGTAA

Upstream 100 bases:

>100_bases
CCGGGTGCGGTATTGTGGAAGACTCGGAACCAATCTCGGAATATGAAGAAACACAGATTAAATTGAAGCCGATGATCTCT
GCATTAGGAGGTGAGAGGCG

Downstream 100 bases:

>100_bases
ACATAACGGTATCAGATGGTGTCCGATATGCAGTGGCAGACGAGGGACCGAATGCTTCCGAAGCCGTCGTCTGTCTGCAT
GGGTTTACCGGCAGCAAACA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MTVNPITHYIGSFIDEFALSGITDAVVCPGSRSTPLAVLCAAHPDISVHVQIDERSAGFFALGLAKAKQRPVLLICTSGT
AAANFYPAVVEAHYSRVPIIVLTADRPHELREVGAPQAINQHFLFGNFVKFFTDSALPEESPQMLRYIRTLASRAAGEAQ
KRPMGPVHVNVPLREPLMPDLSDEPFGRMRTGRHVSVKTGTQSVDRESLSDVAEMLAEAEKGMIVCGELHSDADKENIIA
LSKALQYPILADPLSNLRNGVHDKSTVIDAYDSFLKDDELKRKLRPDVVIRFGPMPVSKPVFLWLKDDPTIQQIVIDEDG
GWRDPTQASAHMIHCNASVFAEEIMAGLTAATRSSEWLEKWQFVNGRFREHLQTISSEDVSFEGNLYRILQHLVPENSSL
FVGNSMPIRDVDTFFEKQDRPFRIYSNRGANGIDGVVSSAMGVCEGTKAPVTLVIGDLSFYHDLNGLLAAKKLGIPLTVI
LVNNDGGGIFSFLPQASEKTHFEDLFGTPTGLDFKHAAALYGGTYSCPASWDEFKTAYAPQADKPGLHLIEIKTDRQSRV
QLHRDMLNEAVREVKKQWEL

Sequences:

>Translated_580_residues
MTVNPITHYIGSFIDEFALSGITDAVVCPGSRSTPLAVLCAAHPDISVHVQIDERSAGFFALGLAKAKQRPVLLICTSGT
AAANFYPAVVEAHYSRVPIIVLTADRPHELREVGAPQAINQHFLFGNFVKFFTDSALPEESPQMLRYIRTLASRAAGEAQ
KRPMGPVHVNVPLREPLMPDLSDEPFGRMRTGRHVSVKTGTQSVDRESLSDVAEMLAEAEKGMIVCGELHSDADKENIIA
LSKALQYPILADPLSNLRNGVHDKSTVIDAYDSFLKDDELKRKLRPDVVIRFGPMPVSKPVFLWLKDDPTIQQIVIDEDG
GWRDPTQASAHMIHCNASVFAEEIMAGLTAATRSSEWLEKWQFVNGRFREHLQTISSEDVSFEGNLYRILQHLVPENSSL
FVGNSMPIRDVDTFFEKQDRPFRIYSNRGANGIDGVVSSAMGVCEGTKAPVTLVIGDLSFYHDLNGLLAAKKLGIPLTVI
LVNNDGGGIFSFLPQASEKTHFEDLFGTPTGLDFKHAAALYGGTYSCPASWDEFKTAYAPQADKPGLHLIEIKTDRQSRV
QLHRDMLNEAVREVKKQWEL
>Mature_579_residues
TVNPITHYIGSFIDEFALSGITDAVVCPGSRSTPLAVLCAAHPDISVHVQIDERSAGFFALGLAKAKQRPVLLICTSGTA
AANFYPAVVEAHYSRVPIIVLTADRPHELREVGAPQAINQHFLFGNFVKFFTDSALPEESPQMLRYIRTLASRAAGEAQK
RPMGPVHVNVPLREPLMPDLSDEPFGRMRTGRHVSVKTGTQSVDRESLSDVAEMLAEAEKGMIVCGELHSDADKENIIAL
SKALQYPILADPLSNLRNGVHDKSTVIDAYDSFLKDDELKRKLRPDVVIRFGPMPVSKPVFLWLKDDPTIQQIVIDEDGG
WRDPTQASAHMIHCNASVFAEEIMAGLTAATRSSEWLEKWQFVNGRFREHLQTISSEDVSFEGNLYRILQHLVPENSSLF
VGNSMPIRDVDTFFEKQDRPFRIYSNRGANGIDGVVSSAMGVCEGTKAPVTLVIGDLSFYHDLNGLLAAKKLGIPLTVIL
VNNDGGGIFSFLPQASEKTHFEDLFGTPTGLDFKHAAALYGGTYSCPASWDEFKTAYAPQADKPGLHLIEIKTDRQSRVQ
LHRDMLNEAVREVKKQWEL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=577, Percent_Identity=29.9826689774697, Blast_Score=234, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_BACSU (P23970)

Other databases:

- EMBL:   M74521
- EMBL:   M74521
- EMBL:   M74538
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69656
- RefSeq:   NP_390960.1
- PDB:   2X7J
- PDBsum:   2X7J
- ProteinModelPortal:   P23970
- SMR:   P23970
- EnsemblBacteria:   EBBACT00000000228
- GeneID:   937198
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30820
- NMPDR:   fig|224308.1.peg.3085
- GenoList:   BSU30820
- GeneTree:   EBGT00050000000626
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- PhylomeDB:   P23970
- ProtClustDB:   PRK07449
- BioCyc:   BSUB:BSU30820-MONOMER
- BioCyc:   MetaCyc:MONOMER-13808
- BRENDA:   2.5.1.64
- BRENDA:   4.1.1.71
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 64093; Mature: 63961

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNPITHYIGSFIDEFALSGITDAVVCPGSRSTPLAVLCAAHPDISVHVQIDERSAGFF
CCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEECCCCCCEE
ALGLAKAKQRPVLLICTSGTAAANFYPAVVEAHYSRVPIIVLTADRPHELREVGAPQAIN
EEEHHHCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCHHHH
QHFLFGNFVKFFTDSALPEESPQMLRYIRTLASRAAGEAQKRPMGPVHVNVPLREPLMPD
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCCCCCCCC
LSDEPFGRMRTGRHVSVKTGTQSVDRESLSDVAEMLAEAEKGMIVCGELHSDADKENIIA
CCCCCCCHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHH
LSKALQYPILADPLSNLRNGVHDKSTVIDAYDSFLKDDELKRKLRPDVVIRFGPMPVSKP
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEECCCCCCCC
VFLWLKDDPTIQQIVIDEDGGWRDPTQASAHMIHCNASVFAEEIMAGLTAATRSSEWLEK
EEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHH
WQFVNGRFREHLQTISSEDVSFEGNLYRILQHLVPENSSLFVGNSMPIRDVDTFFEKQDR
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCC
PFRIYSNRGANGIDGVVSSAMGVCEGTKAPVTLVIGDLSFYHDLNGLLAAKKLGIPLTVI
CEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEE
LVNNDGGGIFSFLPQASEKTHFEDLFGTPTGLDFKHAAALYGGTYSCPASWDEFKTAYAP
EEECCCCCHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHCCC
QADKPGLHLIEIKTDRQSRVQLHRDMLNEAVREVKKQWEL
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVNPITHYIGSFIDEFALSGITDAVVCPGSRSTPLAVLCAAHPDISVHVQIDERSAGFF
CCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEECCCCCCEE
ALGLAKAKQRPVLLICTSGTAAANFYPAVVEAHYSRVPIIVLTADRPHELREVGAPQAIN
EEEHHHCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCHHHH
QHFLFGNFVKFFTDSALPEESPQMLRYIRTLASRAAGEAQKRPMGPVHVNVPLREPLMPD
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCCCCCCCC
LSDEPFGRMRTGRHVSVKTGTQSVDRESLSDVAEMLAEAEKGMIVCGELHSDADKENIIA
CCCCCCCHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHH
LSKALQYPILADPLSNLRNGVHDKSTVIDAYDSFLKDDELKRKLRPDVVIRFGPMPVSKP
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEECCCCCCCC
VFLWLKDDPTIQQIVIDEDGGWRDPTQASAHMIHCNASVFAEEIMAGLTAATRSSEWLEK
EEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHH
WQFVNGRFREHLQTISSEDVSFEGNLYRILQHLVPENSSLFVGNSMPIRDVDTFFEKQDR
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCC
PFRIYSNRGANGIDGVVSSAMGVCEGTKAPVTLVIGDLSFYHDLNGLLAAKKLGIPLTVI
CEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEE
LVNNDGGGIFSFLPQASEKTHFEDLFGTPTGLDFKHAAALYGGTYSCPASWDEFKTAYAP
EEECCCCCHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHCCC
QADKPGLHLIEIKTDRQSRVQLHRDMLNEAVREVKKQWEL
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377