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The map label for this gene is menC

Identifier: 16080130

GI number: 16080130

Start: 3146238

End: 3147353

Strand: Reverse

Name: menC

Synonym: BSU30780

Alternate gene names: 16080130

Gene position: 3147353-3146238 (Counterclockwise)

Preceding gene: 16080131

Following gene: 16080129

Centisome position: 74.66

GC content: 44.09

Gene sequence:

>1116_bases
ATGATCGAAATAGAAAAAATCACGCTTTACCATTTGAGCATGAACTTAAAGAAACCGTTTAAAAACAGTATCGAAACGCT
TCAAGAGCGTAAATTTCTGATCGTGGAAGCCATTGATACATCAGGTGTCACAGGCTGGGGAGAAGTTTCCGCTTTCTCTT
CGCCTTGGTACACCGAGGAAACCATTGGAACATGTTTGCATATGCTAAAAGATTTTTTTATCCCAAATGTCGTCGGCCGT
GAATTCAATCACCCTTCAGAAGTGCCGGACAGCCTGGCGCGGTATAAAGGAAACCGGATGGCAAAAGCGGGACTTGAATC
GGCTGTATGGGATATTTATGCCAAAAAGAAGGGCGTATCCCTTGCTGAGGCATTGGGCGGAACAAGAGATAAAGTCCCTG
CCGGGGTGGTAGTCGGCCTGGCGCCTCTTGACGATATGCTGAAGGAAATTGAGAGCTATCAAAAAGAAGGTTATCAAAGA
ATAAAAATTAAAATTCAGCCTGGGCAGGATGTGGAATTGGTCAAGGCCATCCGCAGCCGATTCCCAACCATCCCGCTTAT
GGCCGATGCCAATTCCTCTTACGAACTGAAGGATATCAGCCGTTTAAAGGAGCTTGATGACTATCATTTAATGATGATTG
AGCAGCCGCTCCAAGCTGACGATATCGTTGACCACCGCCACTTGCAGAAACATTTGAAAACGGCCATTTGCCTTGATGAG
AGCATTTGTTCAGTTGATGATGCGAGACGGGCGATTGAGCTAGGAAGCTGCAAAATTATTAATATAAAACCTTCTCGTGT
CGGCGGCTTAACAGAAGCCTTAAAAATTCATGATTTATGCAAAGAGCATCATATGCAGGTTTGGTGCGGAGGAATGCTGG
AAACAGGCATATCAAGAGCGCAGAACGTGGCGCTTGCTTCACTGCCTCAATTCACAATACCTGGCGATATATCTTCATCC
TCACGCTATTGGGATGAAGATATTGTAACTCCTGATATTCGTATTGATAATGGATTTATTTCTGTGTCAAAACAGCCTGG
TTTAGGGGTTGAGGTAAACCAAGATATTATGCGAAAATATGTGACCAAAATGGATGTTTTCACACAGCATGGTTAA

Upstream 100 bases:

>100_bases
AAATTCCGGCAAAATTCTTTGTGCTTGACCGCCTGCCGCGCAATGCGTCTAATAAGCTCTTGCGAAATCAGCTGAAGGAT
GCGCGTAAAGGAGAACTGCT

Downstream 100 bases:

>100_bases
GAAAAAAGCATTTTGCTATTTTGAATAAATGACACTGTGCTGTTACAGTAAAAGTGTTCGTTGAATACATCCCTTCTCGT
TTGGACCGGCTGCTGACCCA

Product: O-succinylbenzoate-CoA synthase

Products: NA

Alternate protein names: OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; o-succinylbenzoic acid synthase

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIEIEKITLYHLSMNLKKPFKNSIETLQERKFLIVEAIDTSGVTGWGEVSAFSSPWYTEETIGTCLHMLKDFFIPNVVGR
EFNHPSEVPDSLARYKGNRMAKAGLESAVWDIYAKKKGVSLAEALGGTRDKVPAGVVVGLAPLDDMLKEIESYQKEGYQR
IKIKIQPGQDVELVKAIRSRFPTIPLMADANSSYELKDISRLKELDDYHLMMIEQPLQADDIVDHRHLQKHLKTAICLDE
SICSVDDARRAIELGSCKIINIKPSRVGGLTEALKIHDLCKEHHMQVWCGGMLETGISRAQNVALASLPQFTIPGDISSS
SRYWDEDIVTPDIRIDNGFISVSKQPGLGVEVNQDIMRKYVTKMDVFTQHG

Sequences:

>Translated_371_residues
MIEIEKITLYHLSMNLKKPFKNSIETLQERKFLIVEAIDTSGVTGWGEVSAFSSPWYTEETIGTCLHMLKDFFIPNVVGR
EFNHPSEVPDSLARYKGNRMAKAGLESAVWDIYAKKKGVSLAEALGGTRDKVPAGVVVGLAPLDDMLKEIESYQKEGYQR
IKIKIQPGQDVELVKAIRSRFPTIPLMADANSSYELKDISRLKELDDYHLMMIEQPLQADDIVDHRHLQKHLKTAICLDE
SICSVDDARRAIELGSCKIINIKPSRVGGLTEALKIHDLCKEHHMQVWCGGMLETGISRAQNVALASLPQFTIPGDISSS
SRYWDEDIVTPDIRIDNGFISVSKQPGLGVEVNQDIMRKYVTKMDVFTQHG
>Mature_371_residues
MIEIEKITLYHLSMNLKKPFKNSIETLQERKFLIVEAIDTSGVTGWGEVSAFSSPWYTEETIGTCLHMLKDFFIPNVVGR
EFNHPSEVPDSLARYKGNRMAKAGLESAVWDIYAKKKGVSLAEALGGTRDKVPAGVVVGLAPLDDMLKEIESYQKEGYQR
IKIKIQPGQDVELVKAIRSRFPTIPLMADANSSYELKDISRLKELDDYHLMMIEQPLQADDIVDHRHLQKHLKTAICLDE
SICSVDDARRAIELGSCKIINIKPSRVGGLTEALKIHDLCKEHHMQVWCGGMLETGISRAQNVALASLPQFTIPGDISSS
SRYWDEDIVTPDIRIDNGFISVSKQPGLGVEVNQDIMRKYVTKMDVFTQHG

Specific function: Converts SHCHC to OSB

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family

Homologues:

Organism=Escherichia coli, GI87081876, Length=241, Percent_Identity=31.1203319502075, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENC_BACSU (O34514)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   F69991
- RefSeq:   NP_390956.1
- ProteinModelPortal:   O34514
- SMR:   O34514
- EnsemblBacteria:   EBBACT00000001017
- GeneID:   937201
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30780
- NMPDR:   fig|224308.1.peg.3081
- GenoList:   BSU30780
- GeneTree:   EBGT00050000001324
- HOGENOM:   HBG292917
- OMA:   NFTIPGD
- PhylomeDB:   O34514
- ProtClustDB:   CLSK887764
- BioCyc:   BSUB:BSU30780-MONOMER
- BioCyc:   MetaCyc:MONOMER-13796
- BRENDA:   4.2.1.113
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- InterPro:   IPR010197
- PANTHER:   PTHR13794:SF8
- PANTHER:   PTHR13794
- SMART:   SM00922
- TIGRFAMs:   TIGR01928

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: =4.2.1.113

Molecular weight: Translated: 41629; Mature: 41629

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: ACT_SITE 164-164 ACT_SITE 263-263

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIEKITLYHLSMNLKKPFKNSIETLQERKFLIVEAIDTSGVTGWGEVSAFSSPWYTEE
CEEEEEEEEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCHH
TIGTCLHMLKDFFIPNVVGREFNHPSEVPDSLARYKGNRMAKAGLESAVWDIYAKKKGVS
HHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCH
LAEALGGTRDKVPAGVVVGLAPLDDMLKEIESYQKEGYQRIKIKIQPGQDVELVKAIRSR
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
FPTIPLMADANSSYELKDISRLKELDDYHLMMIEQPLQADDIVDHRHLQKHLKTAICLDE
CCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
SICSVDDARRAIELGSCKIINIKPSRVGGLTEALKIHDLCKEHHMQVWCGGMLETGISRA
HHHCHHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHHHHHHCCHHEEECHHHHHHHHHH
QNVALASLPQFTIPGDISSSSRYWDEDIVTPDIRIDNGFISVSKQPGLGVEVNQDIMRKY
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCEECHHHHHHH
VTKMDVFTQHG
HHHHHHHHCCC
>Mature Secondary Structure
MIEIEKITLYHLSMNLKKPFKNSIETLQERKFLIVEAIDTSGVTGWGEVSAFSSPWYTEE
CEEEEEEEEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCHH
TIGTCLHMLKDFFIPNVVGREFNHPSEVPDSLARYKGNRMAKAGLESAVWDIYAKKKGVS
HHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCH
LAEALGGTRDKVPAGVVVGLAPLDDMLKEIESYQKEGYQRIKIKIQPGQDVELVKAIRSR
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
FPTIPLMADANSSYELKDISRLKELDDYHLMMIEQPLQADDIVDHRHLQKHLKTAICLDE
CCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
SICSVDDARRAIELGSCKIINIKPSRVGGLTEALKIHDLCKEHHMQVWCGGMLETGISRA
HHHCHHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHHHHHHCCHHEEECHHHHHHHHHH
QNVALASLPQFTIPGDISSSSRYWDEDIVTPDIRIDNGFISVSKQPGLGVEVNQDIMRKY
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCEECHHHHHHH
VTKMDVFTQHG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10194342