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The map label for this gene is braB

Identifier: 16080012

GI number: 16080012

Start: 3028297

End: 3029634

Strand: Direct

Name: braB

Synonym: BSU29600

Alternate gene names: 16080012

Gene position: 3028297-3029634 (Clockwise)

Preceding gene: 16080006

Following gene: 16080014

Centisome position: 71.84

GC content: 42.53

Gene sequence:

>1338_bases
ATGAAACACTCACTGCCTGTCAAAGATACCATTATTATTGGTTTCATGCTATTTGCGTTATTCTTTGGAGCAGGAAATAT
GATTTATCCGCCGGAACTTGGACAAGCAGCCGGACACAACGTCTGGAAGGCCATCGGCGGATTTTTACTGACAGGGGTAG
GCCTGCCGCTTCTCGGCATTATCGCCATCGCATTAACCGGTAAGGACGCTAAAGGCCTTGCTGACAAAGCGCACCCTGTT
TTCGGCACAATCTTTACTGTCGTGCTTTATTTATCAATCGGACCATTATTCGCCATCCCGAGAACAGGAACTGTTTCATA
TGAAATTGGCGCTGTTCCGTTTTTGACTGGTGTACCAGAAAGACTTTCTTTATTAATATTTACACTTATCTTTTTCGGCG
TTACGTACTATTTAGCGTTAAACCCATCTAAGGTTGTGGATCGAGTCGGAAAAATTCTGACTCCCATCAAATTCACAATT
ATTTTAATCATTGTTCTCAAAGCCATCTTCACGCCGATGGGCGGATTGGGCGCCGTGACAGAAGCATATAAAGGAACACC
GGTTTTTAAAGGATTTTTAGAAGGCTATAAAACAATGGACGCACTTGCTTCAATTGTATTTGGCGTTGTCGTTGTAAATG
CTGTAAAAAGCAAAGGCGTTACACAGTCTAAAGCTCTTGCTGCAGCCTGCATTAAAGCAGGTGTGATTGCCGCTTTAGGC
CTGACTTTCATTTATGTATCCCTTGCTTATTTAGGTGCAACAAGCACAAATGCCATTGGTCCTGTTGGTGAAGGAGCTAA
AATTTTATCTGCGTCTTCCCATTATTTATTCGGTTCATTGGGCAACATCGTACTCGGAGCAGCGATTACAGTCGCATGTT
TAACAACAAGCATCGGCCTTGTCACATCTTGCGGCCAATATTTTTCAAAACTTATTCCGGCATTATCATATAAAATTGTC
GTTACGATTGTCACGTTATTCAGCTTGATCATTGCGAATTTCGGACTCGCGCAAATTATCGCGTTTTCTGTTCCTATTTT
ATCAGCTATCTATCCGCTTGCCATTGTCATTATCGTTCTTTCCTTCATCGATAAGATCTTTAAAGAAAGACGCGAAGTGT
ACATCGCATGCTTGATCGGTACAGGACTTTTCAGTATTTTAGACGGTATCAAAGCAGCCGGTTTTTCACTTGGATCTTTA
GATGTGTTTCTAAATGCGAATCTTCCTCTTTACAGCCTCGGCATCGGCTGGGTGCTTCCAGGAATCGTCGGAGCGGTTAT
CGGCTATGTGCTGACCTTATTCATCGGACCATCCAAACAGCTTAATGAAATAAGTTAA

Upstream 100 bases:

>100_bases
CTAATTTTTTTACCATATAATAAGATTTGTTCGTTTCGTCATATTATCTGACAATTAATTGACAGAATATTTTAACATAA
TTATTAGGAGGATTTTTTTC

Downstream 100 bases:

>100_bases
TGTCACAGAACGCCTGCGTTATTGCGCAGGCGTTTTGTAATAAAAAAAGAGCCTGCTCATTACACTGCGGGCTCTTTTTC
ATGGTCGTGATTATCTAAGC

Product: branched-chain amino acid-Na+ symporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKHSLPVKDTIIIGFMLFALFFGAGNMIYPPELGQAAGHNVWKAIGGFLLTGVGLPLLGIIAIALTGKDAKGLADKAHPV
FGTIFTVVLYLSIGPLFAIPRTGTVSYEIGAVPFLTGVPERLSLLIFTLIFFGVTYYLALNPSKVVDRVGKILTPIKFTI
ILIIVLKAIFTPMGGLGAVTEAYKGTPVFKGFLEGYKTMDALASIVFGVVVVNAVKSKGVTQSKALAAACIKAGVIAALG
LTFIYVSLAYLGATSTNAIGPVGEGAKILSASSHYLFGSLGNIVLGAAITVACLTTSIGLVTSCGQYFSKLIPALSYKIV
VTIVTLFSLIIANFGLAQIIAFSVPILSAIYPLAIVIIVLSFIDKIFKERREVYIACLIGTGLFSILDGIKAAGFSLGSL
DVFLNANLPLYSLGIGWVLPGIVGAVIGYVLTLFIGPSKQLNEIS

Sequences:

>Translated_445_residues
MKHSLPVKDTIIIGFMLFALFFGAGNMIYPPELGQAAGHNVWKAIGGFLLTGVGLPLLGIIAIALTGKDAKGLADKAHPV
FGTIFTVVLYLSIGPLFAIPRTGTVSYEIGAVPFLTGVPERLSLLIFTLIFFGVTYYLALNPSKVVDRVGKILTPIKFTI
ILIIVLKAIFTPMGGLGAVTEAYKGTPVFKGFLEGYKTMDALASIVFGVVVVNAVKSKGVTQSKALAAACIKAGVIAALG
LTFIYVSLAYLGATSTNAIGPVGEGAKILSASSHYLFGSLGNIVLGAAITVACLTTSIGLVTSCGQYFSKLIPALSYKIV
VTIVTLFSLIIANFGLAQIIAFSVPILSAIYPLAIVIIVLSFIDKIFKERREVYIACLIGTGLFSILDGIKAAGFSLGSL
DVFLNANLPLYSLGIGWVLPGIVGAVIGYVLTLFIGPSKQLNEIS
>Mature_445_residues
MKHSLPVKDTIIIGFMLFALFFGAGNMIYPPELGQAAGHNVWKAIGGFLLTGVGLPLLGIIAIALTGKDAKGLADKAHPV
FGTIFTVVLYLSIGPLFAIPRTGTVSYEIGAVPFLTGVPERLSLLIFTLIFFGVTYYLALNPSKVVDRVGKILTPIKFTI
ILIIVLKAIFTPMGGLGAVTEAYKGTPVFKGFLEGYKTMDALASIVFGVVVVNAVKSKGVTQSKALAAACIKAGVIAALG
LTFIYVSLAYLGATSTNAIGPVGEGAKILSASSHYLFGSLGNIVLGAAITVACLTTSIGLVTSCGQYFSKLIPALSYKIV
VTIVTLFSLIIANFGLAQIIAFSVPILSAIYPLAIVIIVLSFIDKIFKERREVYIACLIGTGLFSILDGIKAAGFSLGSL
DVFLNANLPLYSLGIGWVLPGIVGAVIGYVLTLFIGPSKQLNEIS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family

Homologues:

Organism=Escherichia coli, GI1786601, Length=429, Percent_Identity=41.958041958042, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BRAB_BACSU (O34545)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   C69596
- RefSeq:   NP_390838.1
- ProteinModelPortal:   O34545
- EnsemblBacteria:   EBBACT00000001565
- GeneID:   937334
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29600
- NMPDR:   fig|224308.1.peg.2963
- GenoList:   BSU29600
- GeneTree:   EBGT00070000031949
- HOGENOM:   HBG533860
- OMA:   ASSHYLF
- PhylomeDB:   O34545
- ProtClustDB:   CLSK887725
- BioCyc:   BSUB:BSU29600-MONOMER
- InterPro:   IPR004685
- TIGRFAMs:   TIGR00796

Pfam domain/function: PF05525 Branch_AA_trans

EC number: NA

Molecular weight: Translated: 46610; Mature: 46610

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xeb93e4c)-; HASH(0x117d6160)-; HASH(0x11a56210)-; HASH(0x11821c3c)-; HASH(0xece5360)-; HASH(0x11e00344)-; HASH(0x11545e4c)-; HASH(0x10005584)-; HASH(0x119da0d0)-; HASH(0xe6ac1f8)-; HASH(0x1163bd80)-; HASH(0xde382b4)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCHHHCCCH
>Mature Secondary Structure
MKHSLPVKDTIIIGFMLFALFFGAGNMIYPPELGQAAGHNVWKAIGGFLLTGVGLPLLGI
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAIALTGKDAKGLADKAHPVFGTIFTVVLYLSIGPLFAIPRTGTVSYEIGAVPFLTGVPE
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCEEEECCCCHHHCCCHH
RLSLLIFTLIFFGVTYYLALNPSKVVDRVGKILTPIKFTIILIIVLKAIFTPMGGLGAVT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EAYKGTPVFKGFLEGYKTMDALASIVFGVVVVNAVKSKGVTQSKALAAACIKAGVIAALG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LTFIYVSLAYLGATSTNAIGPVGEGAKILSASSHYLFGSLGNIVLGAAITVACLTTSIGL
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VTSCGQYFSKLIPALSYKIVVTIVTLFSLIIANFGLAQIIAFSVPILSAIYPLAIVIIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SFIDKIFKERREVYIACLIGTGLFSILDGIKAAGFSLGSLDVFLNANLPLYSLGIGWVLP
HHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
GIVGAVIGYVLTLFIGPSKQLNEIS
HHHHHHHHHHHHHHCCCCHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377