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The map label for this gene is ackA

Identifier: 16079999

GI number: 16079999

Start: 3015111

End: 3016298

Strand: Reverse

Name: ackA

Synonym: BSU29470

Alternate gene names: 16079999

Gene position: 3016298-3015111 (Counterclockwise)

Preceding gene: 16080000

Following gene: 16079998

Centisome position: 71.55

GC content: 45.03

Gene sequence:

>1188_bases
ATGTCCAAAATTATTGCAATTAACGCAGGAAGCTCGTCTTTGAAATTTCAGCTTTTCGAAATGCCTTCAGAAACCGTTTT
AACGAAGGGTTTAGTTGAACGAATCGGTATCGCCGACAGCGTATTCACAATTTCTGTGAACGGCGAAAAAAATACAGAAG
TAACTGATATTCCAGATCATGCGGTAGCTGTTAAAATGCTGCTGAATAAGTTAACGGAATTCGGCATTATTAAAGACTTG
AATGAAATTGACGGAATTGGCCATCGTGTCGTTCACGGCGGAGAAAAATTCAGCGATTCTGTTTTATTAACGGATGAAAC
CATTAAGGAAATCGAAGATATTTCTGAATTGGCACCGCTTCACAATCCGGCAAATATCGTTGGAATTAAAGCGTTCAAAG
AAGTGCTTCCAAATGTTCCTGCGGTAGCTGTATTTGATACAGCATTCCACCAAACAATGCCTGAGCAGTCTTACCTTTAC
AGCTTGCCGTATGAATACTATGAAAAATTCGGCATCCGTAAATACGGCTTCCACGGCACTTCACATAAATATGTAACTGA
GCGTGCGGCAGAGCTTCTCGGCCGTCCGTTAAAAGACTTGCGCCTGATTTCCTGCCACCTTGGAAACGGAGCAAGTATTG
CCGCTGTTGAAGGCGGAAAATCTATTGACACATCCATGGGCTTTACGCCGCTTGCCGGTGTAGCAATGGGCACACGCTCT
GGAAACATCGACCCTGCCCTGATCCCATACATCATGGAGAAAACAGGCCAAACGGCTGACGAAGTACTGAATACATTAAA
CAAAAAAAGCGGACTGCTCGGCATTTCCGGTTTCTCAAGCGATCTTCGTGACATCGTTGAAGCTACGAAAGAAGGAAATG
AGCGCGCGGAAACGGCTCTTGAAGTATTCGCAAGCAGAATCCACAAATACATCGGTTCTTACGCTGCAAGAATGAGCGGT
GTAGATGCGATCATCTTTACTGCCGGTATCGGTGAAAACAGTGTGGAAGTCAGAGAACGCGTTCTTCGCGGTTTAGAATT
CATGGGCGTATACTGGGACCCTGCGCTTAATAACGTGCGCGGCGAGGAAGCTTTCATCAGCTATCCGCATTCTCCAGTAA
AAGTCATGATCATCCCGACTGATGAAGAAGTCATGATTGCGCGCGACGTTGTTCGTTTAGCAAAATAA

Upstream 100 bases:

>100_bases
ATGGAAAAGTCAGATATTTTTCGGAGAAGATGTTGATATTCACCGGGAAAAAAGTACGAAAAACGAAGTTGTTGATACAG
AGTATAAAGGAGCGTCAATC

Downstream 100 bases:

>100_bases
ATCGCATGAAAGCACATTCTCTTGAATGTGCTTTTTTGTTGATGCATGATAGTGACAGACAGAAAACATAAAAGAGGTGG
GCCAATGAGCGTTCAAGAGC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGIADSVFTISVNGEKNTEVTDIPDHAVAVKMLLNKLTEFGIIKDL
NEIDGIGHRVVHGGEKFSDSVLLTDETIKEIEDISELAPLHNPANIVGIKAFKEVLPNVPAVAVFDTAFHQTMPEQSYLY
SLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLKDLRLISCHLGNGASIAAVEGGKSIDTSMGFTPLAGVAMGTRS
GNIDPALIPYIMEKTGQTADEVLNTLNKKSGLLGISGFSSDLRDIVEATKEGNERAETALEVFASRIHKYIGSYAARMSG
VDAIIFTAGIGENSVEVRERVLRGLEFMGVYWDPALNNVRGEEAFISYPHSPVKVMIIPTDEEVMIARDVVRLAK

Sequences:

>Translated_395_residues
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGIADSVFTISVNGEKNTEVTDIPDHAVAVKMLLNKLTEFGIIKDL
NEIDGIGHRVVHGGEKFSDSVLLTDETIKEIEDISELAPLHNPANIVGIKAFKEVLPNVPAVAVFDTAFHQTMPEQSYLY
SLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLKDLRLISCHLGNGASIAAVEGGKSIDTSMGFTPLAGVAMGTRS
GNIDPALIPYIMEKTGQTADEVLNTLNKKSGLLGISGFSSDLRDIVEATKEGNERAETALEVFASRIHKYIGSYAARMSG
VDAIIFTAGIGENSVEVRERVLRGLEFMGVYWDPALNNVRGEEAFISYPHSPVKVMIIPTDEEVMIARDVVRLAK
>Mature_394_residues
SKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGIADSVFTISVNGEKNTEVTDIPDHAVAVKMLLNKLTEFGIIKDLN
EIDGIGHRVVHGGEKFSDSVLLTDETIKEIEDISELAPLHNPANIVGIKAFKEVLPNVPAVAVFDTAFHQTMPEQSYLYS
LPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLKDLRLISCHLGNGASIAAVEGGKSIDTSMGFTPLAGVAMGTRSG
NIDPALIPYIMEKTGQTADEVLNTLNKKSGLLGISGFSSDLRDIVEATKEGNERAETALEVFASRIHKYIGSYAARMSGV
DAIIFTAGIGENSVEVRERVLRGLEFMGVYWDPALNNVRGEEAFISYPHSPVKVMIIPTDEEVMIARDVVRLAK

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=397, Percent_Identity=44.8362720403023, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=43.3249370277078, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43138; Mature: 43007

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGIADSVFTISVNGEKNTEVTDIPDH
CCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHH
AVAVKMLLNKLTEFGIIKDLNEIDGIGHRVVHGGEKFSDSVLLTDETIKEIEDISELAPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHCC
HNPANIVGIKAFKEVLPNVPAVAVFDTAFHQTMPEQSYLYSLPYEYYEKFGIRKYGFHGT
CCCCCEEHHHHHHHHCCCCCEEEEEHHHHHHCCCCCHHEEECCHHHHHHCCCEEECCCCC
SHKYVTERAAELLGRPLKDLRLISCHLGNGASIAAVEGGKSIDTSMGFTPLAGVAMGTRS
CCHHHHHHHHHHHCCCHHHHEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHEECCCC
GNIDPALIPYIMEKTGQTADEVLNTLNKKSGLLGISGFSSDLRDIVEATKEGNERAETAL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHHH
EVFASRIHKYIGSYAARMSGVDAIIFTAGIGENSVEVRERVLRGLEFMGVYWDPALNNVR
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCHHCCCC
GEEAFISYPHSPVKVMIIPTDEEVMIARDVVRLAK
CCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGIADSVFTISVNGEKNTEVTDIPDH
CEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHH
AVAVKMLLNKLTEFGIIKDLNEIDGIGHRVVHGGEKFSDSVLLTDETIKEIEDISELAPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHCC
HNPANIVGIKAFKEVLPNVPAVAVFDTAFHQTMPEQSYLYSLPYEYYEKFGIRKYGFHGT
CCCCCEEHHHHHHHHCCCCCEEEEEHHHHHHCCCCCHHEEECCHHHHHHCCCEEECCCCC
SHKYVTERAAELLGRPLKDLRLISCHLGNGASIAAVEGGKSIDTSMGFTPLAGVAMGTRS
CCHHHHHHHHHHHCCCHHHHEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHEECCCC
GNIDPALIPYIMEKTGQTADEVLNTLNKKSGLLGISGFSSDLRDIVEATKEGNERAETAL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHHH
EVFASRIHKYIGSYAARMSGVDAIIFTAGIGENSVEVRERVLRGLEFMGVYWDPALNNVR
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCHHCCCC
GEEAFISYPHSPVKVMIIPTDEEVMIARDVVRLAK
CCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA