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The map label for this gene is argG

Identifier: 16079997

GI number: 16079997

Start: 3013133

End: 3014344

Strand: Reverse

Name: argG

Synonym: BSU29450

Alternate gene names: 16079997

Gene position: 3014344-3013133 (Counterclockwise)

Preceding gene: 16079998

Following gene: 16079996

Centisome position: 71.5

GC content: 47.03

Gene sequence:

>1212_bases
ATGTCAGAACAGAAAAAAGTCGTATTAGCATACTCAGGAGGTCTTGATACTTCCGTCGCAATAAAATGGCTTCAGGAGCA
AGGATATAATGTAATCGCCTGCTGCCTGGATGTCGGTGAGGGCAAAGATTTGGCATTTGTTCAGCAGAAAGCGCTTGAAG
TCGGCGCGACAAACTCCTACGTGATCGATGCAAAAGAAGAATTTGCACAGGATTACGCGCTTATCTCTTTGCAGGCCCAC
ACAATGTATGAAGGAAAATACCCGCTTGTTTCGGCATTATCTCGACCGCTGATCGCGAAAAAACTGGTTGAAATTGCGGA
AAAAGAAGATGCACAGGCGATTGCCCACGGATGCACAGGGAAAGGAAATGACCAGGTTCGTTTCGAAGTCTCCATTAAAT
CATTAAACCCTGACCTAGAAGTCATCGCGCCGGTTCGCGAATGGCAGTGGTCACGTGAAGAAGAAATTGAATATGCGGCT
AGCCGCGGCATTCCGATTCCGATCAATCTTGACAGCCCGTATTCTATCGACCAAAACCTCTGGGGACGCGCCAATGAGTG
CGGCATTTTAGAAGATCCATGGGCGGCGCCGCCAGAAGGTGCATACGATTTAACAGCTCCGCTGGAAAAAACGCCGGACA
CGCCGGAAGTGATTGAAATTGCATTTGAACAGGGCGTGCCTGTCTCCATTGACGGTGTTTCCTACTCGCTTTCTGAGCTG
ATCTTGAAGCTGAATGAAATGGCGGGCGCGCACGGTGTCGGACGTATCGACCACGTGGAAAACCGCCTCGTCGGCATTAA
ATCCCGGGAAGTATACGAATGCCCTGGTGCGATGACACTGATCAAAGCGCATAAAGAATTAGAAGATTTGACACTGGTGA
AAGAGGTAGCCCACTTTAAACCGATCATCGAGCAAAAAATGTCAGAAATCATTTACAACGGCTTATGGTTCTCACCGTTA
AAAGACGCGCTGCACGCTTTCTTAAAAGAAACGCAAAAGCATGTCACAGGCATTGTCCGCGTGAAGCTGTTTAAAGGCCA
TGCAATTGTAGAAGGACGTAAATCGGAATACTCTCTATATGATGAAAAACTTGCAACATATACAAAAGACGATGCATTTG
ATCACCATGCAGCAATCGGATTTATCGAGCTTTGGGGACTTCCGACAAAAGTGAACAGCATCGTGAAAAAGAAGGAGCAG
ATTAAAGCATGA

Upstream 100 bases:

>100_bases
GAAACGTACTTGTTAAAGTGTATACATACAAAATGTATTTTTATTAAATATAATTTATTTTTATACAAAATAGAAACACG
GTTGAGAGGGGATTTTTATC

Downstream 100 bases:

>100_bases
AGAAGCTTTGGGGAGGCCGATTTCAAAAAACACCGGAAAAATGGGTCGACGAGTTCGGCGCGTCCATATCATTTGACCAA
AATTTAGTTGCTGAAGACAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEQKKVVLAYSGGLDTSVAIKWLQEQGYNVIACCLDVGEGKDLAFVQQKALEVGATNSYVIDAKEEFAQDYALISLQAH
TMYEGKYPLVSALSRPLIAKKLVEIAEKEDAQAIAHGCTGKGNDQVRFEVSIKSLNPDLEVIAPVREWQWSREEEIEYAA
SRGIPIPINLDSPYSIDQNLWGRANECGILEDPWAAPPEGAYDLTAPLEKTPDTPEVIEIAFEQGVPVSIDGVSYSLSEL
ILKLNEMAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFKPIIEQKMSEIIYNGLWFSPL
KDALHAFLKETQKHVTGIVRVKLFKGHAIVEGRKSEYSLYDEKLATYTKDDAFDHHAAIGFIELWGLPTKVNSIVKKKEQ
IKA

Sequences:

>Translated_403_residues
MSEQKKVVLAYSGGLDTSVAIKWLQEQGYNVIACCLDVGEGKDLAFVQQKALEVGATNSYVIDAKEEFAQDYALISLQAH
TMYEGKYPLVSALSRPLIAKKLVEIAEKEDAQAIAHGCTGKGNDQVRFEVSIKSLNPDLEVIAPVREWQWSREEEIEYAA
SRGIPIPINLDSPYSIDQNLWGRANECGILEDPWAAPPEGAYDLTAPLEKTPDTPEVIEIAFEQGVPVSIDGVSYSLSEL
ILKLNEMAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFKPIIEQKMSEIIYNGLWFSPL
KDALHAFLKETQKHVTGIVRVKLFKGHAIVEGRKSEYSLYDEKLATYTKDDAFDHHAAIGFIELWGLPTKVNSIVKKKEQ
IKA
>Mature_402_residues
SEQKKVVLAYSGGLDTSVAIKWLQEQGYNVIACCLDVGEGKDLAFVQQKALEVGATNSYVIDAKEEFAQDYALISLQAHT
MYEGKYPLVSALSRPLIAKKLVEIAEKEDAQAIAHGCTGKGNDQVRFEVSIKSLNPDLEVIAPVREWQWSREEEIEYAAS
RGIPIPINLDSPYSIDQNLWGRANECGILEDPWAAPPEGAYDLTAPLEKTPDTPEVIEIAFEQGVPVSIDGVSYSLSELI
LKLNEMAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFKPIIEQKMSEIIYNGLWFSPLK
DALHAFLKETQKHVTGIVRVKLFKGHAIVEGRKSEYSLYDEKLATYTKDDAFDHHAAIGFIELWGLPTKVNSIVKKKEQI
KA

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=400, Percent_Identity=42.75, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI16950633, Length=400, Percent_Identity=42.75, Blast_Score=315, Evalue=5e-86,
Organism=Escherichia coli, GI1789563, Length=414, Percent_Identity=28.5024154589372, Blast_Score=133, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=401, Percent_Identity=37.9052369077307, Blast_Score=277, Evalue=2e-75,
Organism=Drosophila melanogaster, GI21358151, Length=414, Percent_Identity=38.1642512077295, Blast_Score=282, Evalue=4e-76,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44818; Mature: 44686

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKKVVLAYSGGLDTSVAIKWLQEQGYNVIACCLDVGEGKDLAFVQQKALEVGATNSY
CCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCE
VIDAKEEFAQDYALISLQAHTMYEGKYPLVSALSRPLIAKKLVEIAEKEDAQAIAHGCTG
EEECHHHHCCCEEEEEEEEEEEECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCC
KGNDQVRFEVSIKSLNPDLEVIAPVREWQWSREEEIEYAASRGIPIPINLDSPYSIDQNL
CCCCEEEEEEEEECCCCCCEEEECHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHH
WGRANECGILEDPWAAPPEGAYDLTAPLEKTPDTPEVIEIAFEQGVPVSIDGVSYSLSEL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHH
ILKLNEMAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFK
HHHHHHHCCCCCCCCHHHHHHHEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PIIEQKMSEIIYNGLWFSPLKDALHAFLKETQKHVTGIVRVKLFKGHAIVEGRKSEYSLY
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEECCCCCCHHHH
DEKLATYTKDDAFDHHAAIGFIELWGLPTKVNSIVKKKEQIKA
HHHHHHCCCCCCCCHHHHEEEHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEQKKVVLAYSGGLDTSVAIKWLQEQGYNVIACCLDVGEGKDLAFVQQKALEVGATNSY
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCE
VIDAKEEFAQDYALISLQAHTMYEGKYPLVSALSRPLIAKKLVEIAEKEDAQAIAHGCTG
EEECHHHHCCCEEEEEEEEEEEECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCC
KGNDQVRFEVSIKSLNPDLEVIAPVREWQWSREEEIEYAASRGIPIPINLDSPYSIDQNL
CCCCEEEEEEEEECCCCCCEEEECHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHH
WGRANECGILEDPWAAPPEGAYDLTAPLEKTPDTPEVIEIAFEQGVPVSIDGVSYSLSEL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHH
ILKLNEMAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFK
HHHHHHHCCCCCCCCHHHHHHHEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PIIEQKMSEIIYNGLWFSPLKDALHAFLKETQKHVTGIVRVKLFKGHAIVEGRKSEYSLY
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEECCCCCCHHHH
DEKLATYTKDDAFDHHAAIGFIELWGLPTKVNSIVKKKEQIKA
HHHHHHCCCCCCCCHHHHEEEHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA