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The map label for this gene is argH

Identifier: 16079996

GI number: 16079996

Start: 3011751

End: 3013136

Strand: Reverse

Name: argH

Synonym: BSU29440

Alternate gene names: 16079996

Gene position: 3013136-3011751 (Counterclockwise)

Preceding gene: 16079997

Following gene: 255767677

Centisome position: 71.48

GC content: 47.62

Gene sequence:

>1386_bases
ATGAAGAAGCTTTGGGGAGGCCGATTTCAAAAAACACCGGAAAAATGGGTCGACGAGTTCGGCGCGTCCATATCATTTGA
CCAAAATTTAGTTGCTGAAGACATTACAGGGTCTCTCGCACATGCTGCCATGCTGAAAAAATGCGGTATTCTGACAGCAG
AAGAAGAGTTGAAAATCCGAGAGGGCCTGAATACCCTTTTGCAAAAAGCAGAGGAGGGAGCATTGGAATTCTCAGTCGAT
TATGAGGATATTCATTTGAACATTGAGAAAATGCTCATTGACGAAATCGGTCCTCTTGGCGGCAAGCTGCATACCGGCAG
AAGCCGGAATGACCAGGTGGCGACTGACATGCATTTATACTTGAAGAATCATGTCGGCCACATCATCGAGCTGATTGAGG
CATTCCAAAGTGCGCTGATTGAAAAGGCGGAAGCGAACGTTGAAACGATTCTGCCGGGCTACACACATTTGCAGCGCGCC
CAGCCGATTTCATTCGCTCACCATCTGCTTGCGTATTTCTGGATGCTTGAGCGCGACAAGGAGCGTTTTCAGGATTCAAT
GAAGCGGATTAACAAATCTCCGCTCGGCTGCGGGGCGCTCGCGGGAACAACCTTCCCAATTGACCGTGATTATTCAGCGG
AACTGCTCGGCTTTGATGCCATTTATGAAAACAGCTTGGACGGCGTCAGCGACAGAGACTTCATTTTGGAGTTTTTAAGC
AACAGCAGTATGCTGATGATGCACCTATCCCGTTTCTCTGAAGAAATCATTTTATGGTGCTCGCAGGAGTTTAAATTCAT
CGAGCTTGACGATACGTACGCAACAGGAAGCAGCATGATGCCGCAAAAGAAAAACCCTGATATGGCGGAGCTGATCCGCG
GCAAAACAGGACGCGTATACGGCGATATGATGGGGCTGTTTACGATCATGAAAGGACTTCCGCTCGCTTACAACAAAGAT
CTGCAGGAAGACAAAGAGGGCATGTTTGACACGGTGAAAACGGTGGAGGGCAGCCTGCAAATCTTCACAGGCATGATCCA
AACAATGACTGTAAACAAAGACGTCATGAAGCAAGCAACAAAACAAGACTTTTCAAACGCGACGGAACTGGCCGATTATT
TAGCTAAAAAAGGAATGCCGTTCAGAGAAGCGCATGAAGTGGTCGGGAAACTTGTGTACACATGCATCGAAAGAGGGATC
TATTTAAGCGATATGCCGTTTGGCGAGTTTCAGCAGGCGAGCACACTATTTGAAGAAGATATTTACACTGTGTTAGACCC
TTACTACGCAGTTGAAAAAAGAATGAGCGCCGGGGGCACCGGATTCAAACAGGTGGAACAGGCGCTTGAAAAAGCGAAAG
CTTGCGTGGCAGCAGGAGTTTGTTAA

Upstream 100 bases:

>100_bases
ACGATGCATTTGATCACCATGCAGCAATCGGATTTATCGAGCTTTGGGGACTTCCGACAAAAGTGAACAGCATCGTGAAA
AAGAAGGAGCAGATTAAAGC

Downstream 100 bases:

>100_bases
AATTTCCTTCATAAAATCCCGTCATTATAGCCAACCTACCTCTATAATGATGGGAGGCAGAATGATGAAAAAAGAACCGG
AACGCTATATTTATGATGAA

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKLWGGRFQKTPEKWVDEFGASISFDQNLVAEDITGSLAHAAMLKKCGILTAEEELKIREGLNTLLQKAEEGALEFSVD
YEDIHLNIEKMLIDEIGPLGGKLHTGRSRNDQVATDMHLYLKNHVGHIIELIEAFQSALIEKAEANVETILPGYTHLQRA
QPISFAHHLLAYFWMLERDKERFQDSMKRINKSPLGCGALAGTTFPIDRDYSAELLGFDAIYENSLDGVSDRDFILEFLS
NSSMLMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVYGDMMGLFTIMKGLPLAYNKD
LQEDKEGMFDTVKTVEGSLQIFTGMIQTMTVNKDVMKQATKQDFSNATELADYLAKKGMPFREAHEVVGKLVYTCIERGI
YLSDMPFGEFQQASTLFEEDIYTVLDPYYAVEKRMSAGGTGFKQVEQALEKAKACVAAGVC

Sequences:

>Translated_461_residues
MKKLWGGRFQKTPEKWVDEFGASISFDQNLVAEDITGSLAHAAMLKKCGILTAEEELKIREGLNTLLQKAEEGALEFSVD
YEDIHLNIEKMLIDEIGPLGGKLHTGRSRNDQVATDMHLYLKNHVGHIIELIEAFQSALIEKAEANVETILPGYTHLQRA
QPISFAHHLLAYFWMLERDKERFQDSMKRINKSPLGCGALAGTTFPIDRDYSAELLGFDAIYENSLDGVSDRDFILEFLS
NSSMLMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVYGDMMGLFTIMKGLPLAYNKD
LQEDKEGMFDTVKTVEGSLQIFTGMIQTMTVNKDVMKQATKQDFSNATELADYLAKKGMPFREAHEVVGKLVYTCIERGI
YLSDMPFGEFQQASTLFEEDIYTVLDPYYAVEKRMSAGGTGFKQVEQALEKAKACVAAGVC
>Mature_461_residues
MKKLWGGRFQKTPEKWVDEFGASISFDQNLVAEDITGSLAHAAMLKKCGILTAEEELKIREGLNTLLQKAEEGALEFSVD
YEDIHLNIEKMLIDEIGPLGGKLHTGRSRNDQVATDMHLYLKNHVGHIIELIEAFQSALIEKAEANVETILPGYTHLQRA
QPISFAHHLLAYFWMLERDKERFQDSMKRINKSPLGCGALAGTTFPIDRDYSAELLGFDAIYENSLDGVSDRDFILEFLS
NSSMLMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVYGDMMGLFTIMKGLPLAYNKD
LQEDKEGMFDTVKTVEGSLQIFTGMIQTMTVNKDVMKQATKQDFSNATELADYLAKKGMPFREAHEVVGKLVYTCIERGI
YLSDMPFGEFQQASTLFEEDIYTVLDPYYAVEKRMSAGGTGFKQVEQALEKAKACVAAGVC

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=456, Percent_Identity=44.2982456140351, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI68303542, Length=456, Percent_Identity=44.2982456140351, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI68303549, Length=456, Percent_Identity=41.6666666666667, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI68303547, Length=456, Percent_Identity=41.6666666666667, Blast_Score=368, Evalue=1e-102,
Organism=Escherichia coli, GI1790398, Length=452, Percent_Identity=49.1150442477876, Blast_Score=454, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6321806, Length=456, Percent_Identity=42.7631578947368, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI221473854, Length=453, Percent_Identity=39.2935982339956, Blast_Score=357, Evalue=7e-99,
Organism=Drosophila melanogaster, GI78706858, Length=453, Percent_Identity=39.2935982339956, Blast_Score=357, Evalue=7e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_BACSU (O34858)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   C69589
- RefSeq:   NP_390822.1
- ProteinModelPortal:   O34858
- SMR:   O34858
- EnsemblBacteria:   EBBACT00000002338
- GeneID:   937966
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29440
- NMPDR:   fig|224308.1.peg.2947
- GenoList:   BSU29440
- GeneTree:   EBGT00070000031828
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- PhylomeDB:   O34858
- ProtClustDB:   PRK00855
- BioCyc:   BSUB:BSU29440-MONOMER
- BRENDA:   4.3.2.1
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 51930; Mature: 51930

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLWGGRFQKTPEKWVDEFGASISFDQNLVAEDITGSLAHAAMLKKCGILTAEEELKIR
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EGLNTLLQKAEEGALEFSVDYEDIHLNIEKMLIDEIGPLGGKLHTGRSRNDQVATDMHLY
HHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
LKNHVGHIIELIEAFQSALIEKAEANVETILPGYTHLQRAQPISFAHHLLAYFWMLERDK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
ERFQDSMKRINKSPLGCGALAGTTFPIDRDYSAELLGFDAIYENSLDGVSDRDFILEFLS
HHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHC
NSSMLMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVY
CCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCHH
GDMMGLFTIMKGLPLAYNKDLQEDKEGMFDTVKTVEGSLQIFTGMIQTMTVNKDVMKQAT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KQDFSNATELADYLAKKGMPFREAHEVVGKLVYTCIERGIYLSDMPFGEFQQASTLFEED
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHH
IYTVLDPYYAVEKRMSAGGTGFKQVEQALEKAKACVAAGVC
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLWGGRFQKTPEKWVDEFGASISFDQNLVAEDITGSLAHAAMLKKCGILTAEEELKIR
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EGLNTLLQKAEEGALEFSVDYEDIHLNIEKMLIDEIGPLGGKLHTGRSRNDQVATDMHLY
HHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
LKNHVGHIIELIEAFQSALIEKAEANVETILPGYTHLQRAQPISFAHHLLAYFWMLERDK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
ERFQDSMKRINKSPLGCGALAGTTFPIDRDYSAELLGFDAIYENSLDGVSDRDFILEFLS
HHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHC
NSSMLMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVY
CCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCHH
GDMMGLFTIMKGLPLAYNKDLQEDKEGMFDTVKTVEGSLQIFTGMIQTMTVNKDVMKQAT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KQDFSNATELADYLAKKGMPFREAHEVVGKLVYTCIERGIYLSDMPFGEFQQASTLFEED
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHH
IYTVLDPYYAVEKRMSAGGTGFKQVEQALEKAKACVAAGVC
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377