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The map label for this gene is citZ

Identifier: 16079966

GI number: 16079966

Start: 2981151

End: 2982269

Strand: Reverse

Name: citZ

Synonym: BSU29140

Alternate gene names: 16079966

Gene position: 2982269-2981151 (Counterclockwise)

Preceding gene: 16079967

Following gene: 16079965

Centisome position: 70.74

GC content: 46.38

Gene sequence:

>1119_bases
ATGACAGCGACACGCGGTCTTGAAGGGGTTGTAGCAACAACATCATCTGTTAGTTCTATTATTGATGATACCCTTACATA
TGTGGGGTATGATATCGATGATTTGACAGAGAATGCAAGTTTTGAAGAAATCATCTACTTGCTTTGGCATCTAAGGCTGC
CAAACAAAAAAGAGCTTGAAGAGTTAAAGCAGCAGCTTGCAAAGGAAGCTGCCGTTCCGCAAGAAATTATTGAGCACTTC
AAATCTTATTCCCTCGAAAATGTTCATCCCATGGCTGCTCTTCGGACAGCAATTTCATTACTAGGCCTGCTTGACAGCGA
GGCTGATACCATGAACCCGGAAGCCAACTACAGAAAGGCGATCCGCCTTCAGGCAAAGGTGCCTGGCCTGGTTGCAGCGT
TTTCCAGAATTCGCAAGGGTCTTGAGCCTGTTGAACCGAGAGAAGATTACGGTATTGCGGAGAATTTTCTTTACACATTA
AACGGTGAAGAGCCTTCACCAATTGAAGTTGAAGCCTTCAACAAAGCATTGATTTTACATGCTGACCATGAGCTGAACGC
GTCAACGTTCACAGCGAGGGTCTGCGTCGCAACACTTTCTGACATCTATTCAGGCATTACTGCAGCAATCGGCGCGCTGA
AAGGGCCTCTTCACGGCGGAGCGAATGAAGGCGTTATGAAAATGCTGACAGAAATCGGTGAAGTGGAAAACGCTGAGCCG
TACATCCGCGCTAAACTTGAAAAGAAAGAAAAAATCATGGGATTCGGCCACCGTGTGTATAAGCATGGTGATCCGCGCGC
GAAGCATCTGAAAGAAATGAGCAAGCGCCTGACCAACCTGACAGGCGAAAGCAAATGGTATGAGATGTCGATTCGTATAG
AAGACATCGTCACATCAGAGAAAAAGCTTCCGCCTAACGTTGATTTCTATTCGGCTTCTGTATATCACAGCCTCGGTATT
GACCATGATTTGTTTACACCGATTTTTGCTGTAAGCAGAATGTCCGGCTGGCTCGCTCATATTCTTGAGCAGTATGACAA
CAACCGTCTGATCCGCCCGCGGGCAGATTACACAGGCCCTGACAAACAAAAATTCGTTCCGATTGAAGAAAGAGCCTAA

Upstream 100 bases:

>100_bases
TTTTTGGGGGAGAGAAATACTTGCATCGTGCTTTTTAAGTAAGCCTCATGTTTTTACAACACTCTTAAAGGGGAAATTTA
TTGAAAAGGAGATGTTATAT

Downstream 100 bases:

>100_bases
AGAACCATTGGAGGCTGGCTTGCGTCAATGAGGTTAGCCTCTTGTTCAGACATCAAAATTGGGTTACACTTTAAATTGAA
TGTTAGGAAAAATCATTTTA

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKKELEELKQQLAKEAAVPQEIIEHF
KSYSLENVHPMAALRTAISLLGLLDSEADTMNPEANYRKAIRLQAKVPGLVAAFSRIRKGLEPVEPREDYGIAENFLYTL
NGEEPSPIEVEAFNKALILHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEGVMKMLTEIGEVENAEP
YIRAKLEKKEKIMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRIEDIVTSEKKLPPNVDFYSASVYHSLGI
DHDLFTPIFAVSRMSGWLAHILEQYDNNRLIRPRADYTGPDKQKFVPIEERA

Sequences:

>Translated_372_residues
MTATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKKELEELKQQLAKEAAVPQEIIEHF
KSYSLENVHPMAALRTAISLLGLLDSEADTMNPEANYRKAIRLQAKVPGLVAAFSRIRKGLEPVEPREDYGIAENFLYTL
NGEEPSPIEVEAFNKALILHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEGVMKMLTEIGEVENAEP
YIRAKLEKKEKIMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRIEDIVTSEKKLPPNVDFYSASVYHSLGI
DHDLFTPIFAVSRMSGWLAHILEQYDNNRLIRPRADYTGPDKQKFVPIEERA
>Mature_371_residues
TATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKKELEELKQQLAKEAAVPQEIIEHFK
SYSLENVHPMAALRTAISLLGLLDSEADTMNPEANYRKAIRLQAKVPGLVAAFSRIRKGLEPVEPREDYGIAENFLYTLN
GEEPSPIEVEAFNKALILHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEGVMKMLTEIGEVENAEPY
IRAKLEKKEKIMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRIEDIVTSEKKLPPNVDFYSASVYHSLGID
HDLFTPIFAVSRMSGWLAHILEQYDNNRLIRPRADYTGPDKQKFVPIEERA

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family

Homologues:

Organism=Homo sapiens, GI38327625, Length=379, Percent_Identity=27.4406332453826, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786527, Length=371, Percent_Identity=37.466307277628, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1786939, Length=377, Percent_Identity=34.4827586206897, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17555174, Length=372, Percent_Identity=26.6129032258064, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=360, Percent_Identity=25.5555555555556, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6319850, Length=330, Percent_Identity=25.1515151515152, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325257, Length=180, Percent_Identity=30, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640124, Length=376, Percent_Identity=27.1276595744681, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24640126, Length=376, Percent_Identity=27.1276595744681, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356863, Length=377, Percent_Identity=26.525198938992, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CISY2_BACSU (P39120)

Other databases:

- EMBL:   U05257
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69600
- RefSeq:   NP_390792.1
- ProteinModelPortal:   P39120
- SMR:   P39120
- PhosSite:   P39120
- EnsemblBacteria:   EBBACT00000000677
- GeneID:   937381
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29140
- NMPDR:   fig|224308.1.peg.2917
- GenoList:   BSU29140
- GeneTree:   EBGT00050000001635
- HOGENOM:   HBG649489
- OMA:   VHPMAAL
- PhylomeDB:   P39120
- ProtClustDB:   PRK14034
- BioCyc:   BSUB:BSU29140-MONOMER
- BRENDA:   2.3.3.1
- GO:   GO:0005737
- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- Gene3D:   G3DSA:1.10.580.10
- Gene3D:   G3DSA:1.10.230.10
- PANTHER:   PTHR11739
- PRINTS:   PR00143
- TIGRFAMs:   TIGR01800

Pfam domain/function: PF00285 Citrate_synt; SSF48256 Citrate_synthase_core

EC number: =2.3.3.1

Molecular weight: Translated: 41729; Mature: 41598

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: ACT_SITE 257-257 ACT_SITE 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKKELE
CCCCCCCCCCEECHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
ELKQQLAKEAAVPQEIIEHFKSYSLENVHPMAALRTAISLLGLLDSEADTMNPEANYRKA
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IRLQAKVPGLVAAFSRIRKGLEPVEPREDYGIAENFLYTLNGEEPSPIEVEAFNKALILH
EEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCEEEEECCCCCCCEEHEECCCEEEEE
ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEGVMKMLTEIGEVENAEP
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCH
YIRAKLEKKEKIMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRIEDIVTSE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHCCC
KKLPPNVDFYSASVYHSLGIDHDLFTPIFAVSRMSGWLAHILEQYDNNRLIRPRADYTGP
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
DKQKFVPIEERA
CCCCCCCCCCCC
>Mature Secondary Structure 
TATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKKELE
CCCCCCCCCEECHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
ELKQQLAKEAAVPQEIIEHFKSYSLENVHPMAALRTAISLLGLLDSEADTMNPEANYRKA
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IRLQAKVPGLVAAFSRIRKGLEPVEPREDYGIAENFLYTLNGEEPSPIEVEAFNKALILH
EEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCEEEEECCCCCCCEEHEECCCEEEEE
ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEGVMKMLTEIGEVENAEP
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCH
YIRAKLEKKEKIMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRIEDIVTSE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHCCC
KKLPPNVDFYSASVYHSLGIDHDLFTPIFAVSRMSGWLAHILEQYDNNRLIRPRADYTGP
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
DKQKFVPIEERA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377