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The map label for this gene is araD

Identifier: 16079930

GI number: 16079930

Start: 2944983

End: 2945672

Strand: Reverse

Name: araD

Synonym: BSU28780

Alternate gene names: 16079930

Gene position: 2945672-2944983 (Counterclockwise)

Preceding gene: 16079931

Following gene: 16079929

Centisome position: 69.88

GC content: 45.36

Gene sequence:

>690_bases
ATGCTTGAAACATTAAAAAAAGAAGTGCTGGCTGCCAACCTGAAGCTTCAAGAGCATCAGCTGGTAACCTTTACGTGGGG
AAATGTCAGCGGCATTGACCGTGAAAAAGAAAGAATTGTCATCAAACCTAGCGGAGTCGAATACAGCGACCTGACAGCCG
ATGACTTGGTTGTTTTGAACCTTGATGGAGAGGTCGTCGAAGGCTCGCTTAAACCTTCTTCAGATACACCTACCCATGTT
TATCTATATAAAGCCTTTCCGAATATCGGGGGAATTGTCCATACCCATTCTCAATGGGCGACAAGCTGGGCGCAATCGGG
CAGAGACATCCCTCCGTTAGGCACGACCCATGCTGATTATTTTGACAGTGCGATTCCATGTACTCGAGAAATGTACGATG
AAGAAATCATTCATGACTACGAACTGAATACAGGAAAAGTCATAGCGGAAACCTTTCAGCATCATAATTACGAACAGGTG
CCGGGTGTGCTCGTGAATAATCACGGACCGTTCTGCTGGGGCACTGACGCCTTAAATGCCATTCATAACGCAGTTGTATT
AGAAACGGTTGCCGAAATGGCCTATCACTCCATTATGCTGAACAAGGATGTAACCCCAATCAATACAGTCCTGCATGAAA
AGCATTTTTATCGAAAACACGGAGCAAATGCGTATTATGGCCAGTCATGA

Upstream 100 bases:

>100_bases
TATTTCGGAAAAGAAAACCATGTCATGAAGCGTCTGAAAACGATCAAAAATCTTCAATTTTCATCTGCCGCCAAAAAGAA
TTGATAAAGGGTGATGGAGC

Downstream 100 bases:

>100_bases
TACGCCTGTGTCACCGGCTGGCATTCTGATTGACTTGGACGGTACTGTATTCAGAGGAAATGAGTTGATCGAAGGAGCAA
GAGAAGCGATCAAAACGCTT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: Phosphoribulose isomerase

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MLETLKKEVLAANLKLQEHQLVTFTWGNVSGIDREKERIVIKPSGVEYSDLTADDLVVLNLDGEVVEGSLKPSSDTPTHV
YLYKAFPNIGGIVHTHSQWATSWAQSGRDIPPLGTTHADYFDSAIPCTREMYDEEIIHDYELNTGKVIAETFQHHNYEQV
PGVLVNNHGPFCWGTDALNAIHNAVVLETVAEMAYHSIMLNKDVTPINTVLHEKHFYRKHGANAYYGQS

Sequences:

>Translated_229_residues
MLETLKKEVLAANLKLQEHQLVTFTWGNVSGIDREKERIVIKPSGVEYSDLTADDLVVLNLDGEVVEGSLKPSSDTPTHV
YLYKAFPNIGGIVHTHSQWATSWAQSGRDIPPLGTTHADYFDSAIPCTREMYDEEIIHDYELNTGKVIAETFQHHNYEQV
PGVLVNNHGPFCWGTDALNAIHNAVVLETVAEMAYHSIMLNKDVTPINTVLHEKHFYRKHGANAYYGQS
>Mature_229_residues
MLETLKKEVLAANLKLQEHQLVTFTWGNVSGIDREKERIVIKPSGVEYSDLTADDLVVLNLDGEVVEGSLKPSSDTPTHV
YLYKAFPNIGGIVHTHSQWATSWAQSGRDIPPLGTTHADYFDSAIPCTREMYDEEIIHDYELNTGKVIAETFQHHNYEQV
PGVLVNNHGPFCWGTDALNAIHNAVVLETVAEMAYHSIMLNKDVTPINTVLHEKHFYRKHGANAYYGQS

Specific function: L-arabinose catabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily

Homologues:

Organism=Escherichia coli, GI1786247, Length=231, Percent_Identity=60.1731601731602, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=59.3073593073593, Blast_Score=274, Evalue=3e-75,
Organism=Escherichia coli, GI1790642, Length=228, Percent_Identity=56.140350877193, Blast_Score=271, Evalue=3e-74,

Paralogues:

None

Copy number: 136 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): ARAD_BACSU (P94525)

Other databases:

- EMBL:   X89408
- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   E69587
- RefSeq:   NP_390756.1
- ProteinModelPortal:   P94525
- SMR:   P94525
- EnsemblBacteria:   EBBACT00000002160
- GeneID:   937894
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28780
- NMPDR:   fig|224308.1.peg.2881
- GenoList:   BSU28780
- GeneTree:   EBGT00050000002575
- HOGENOM:   HBG541069
- OMA:   PCTREMY
- PhylomeDB:   P94525
- ProtClustDB:   PRK08193
- BioCyc:   BSUB:BSU28780-MONOMER
- BRENDA:   5.1.3.4
- InterPro:   IPR001303
- InterPro:   IPR004661
- Gene3D:   G3DSA:3.40.225.10
- TIGRFAMs:   TIGR00760

Pfam domain/function: PF00596 Aldolase_II; SSF53639 Aldolase_II_N

EC number: =5.1.3.4

Molecular weight: Translated: 25686; Mature: 25686

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETLKKEVLAANLKLQEHQLVTFTWGNVSGIDREKERIVIKPSGVEYSDLTADDLVVLN
CHHHHHHHHHHHCCEEECCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEE
LDGEVVEGSLKPSSDTPTHVYLYKAFPNIGGIVHTHSQWATSWAQSGRDIPPLGTTHADY
CCCCEEECCCCCCCCCCCEEEEEECCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCCHHH
FDSAIPCTREMYDEEIIHDYELNTGKVIAETFQHHNYEQVPGVLVNNHGPFCWGTDALNA
HHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHCCCEEEECCCCEEECHHHHHH
IHNAVVLETVAEMAYHSIMLNKDVTPINTVLHEKHFYRKHGANAYYGQS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLETLKKEVLAANLKLQEHQLVTFTWGNVSGIDREKERIVIKPSGVEYSDLTADDLVVLN
CHHHHHHHHHHHCCEEECCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEE
LDGEVVEGSLKPSSDTPTHVYLYKAFPNIGGIVHTHSQWATSWAQSGRDIPPLGTTHADY
CCCCEEECCCCCCCCCCCEEEEEECCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCCHHH
FDSAIPCTREMYDEEIIHDYELNTGKVIAETFQHHNYEQVPGVLVNNHGPFCWGTDALNA
HHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHCCCEEEECCCCEEECHHHHHH
IHNAVVLETVAEMAYHSIMLNKDVTPINTVLHEKHFYRKHGANAYYGQS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9084180; 8969504; 9384377; 10417639