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The map label for this gene is ilvB

Identifier: 16079883

GI number: 16079883

Start: 2895248

End: 2896972

Strand: Reverse

Name: ilvB

Synonym: BSU28310

Alternate gene names: 16079883

Gene position: 2896972-2895248 (Counterclockwise)

Preceding gene: 255767653

Following gene: 255767651

Centisome position: 68.72

GC content: 47.3

Gene sequence:

>1725_bases
ATGGGGACTAATGTACAGGTGGATTCAGCATCTGCCGAATGTACACAGACGATGAGCGGAGCATTAATGCTGATTGAATC
ATTAAAAAAAGAGAAAGTAGAAATGATCTTCGGTTATCCGGGCGGGGCTGTGCTTCCGATTTACGATAAGCTATACAATT
CAGGGTTGGTACATATCCTTCCCCGTCACGAACAAGGAGCAATTCATGCAGCGGAGGGATACGCAAGGGTCTCCGGAAAA
CCGGGTGTCGTCATTGCCACGTCAGGGCCGGGAGCGACAAACCTTGTTACAGGCCTTGCTGATGCCATGATTGATTCATT
GCCGTTAGTCGTCTTTACAGGGCAGGTAGCAACCTCTGTAATCGGGAGCGATGCATTTCAGGAAGCAGACATTTTAGGGA
TTACGATGCCAGTAACAAAACACAGCTACCAGGTTCGCCAGCCGGAAGATCTGCCGCGCATCATTAAAGAAGCGTTCCAT
ATTGCAACAACTGGAAGACCCGGACCTGTATTGATTGATATTCCGAAAGATGTAGCAACAATTGAAGGAGAATTCAGCTA
CGATCATGAGATGAATCTCCCGGGATACCAGCCGACAACAGAGCCGAATTATTTGCAGATCCGCAAGCTTGTGGAAGCCG
TGAGCAGTGCGAAAAAACCGGTGATCCTGGCGGGTGCGGGCGTACTGCACGGAAAAGCGTCAGAAGAATTAAAAAATTAT
GCTGAACAGCAGCAAATCCCTGTGGCACACACCCTTTTGGGGCTCGGAGGCTTCCCGGCTGACCATCCGCTTTTCCTAGG
GATGGCGGGAATGCACGGTACTTATACAGCCAATATGGCCCTTCATGAATGTGATCTATTAATCAGTATCGGCGCCCGTT
TTGATGACCGTGTCACAGGAAACCTGAAACACTTTGCCAGAAACGCAAAGATAGCCCACATCGATATTGATCCAGCTGAA
ATCGGAAAAATCATGAAAACACAGATTCCTGTAGTCGGAGACAGCAAAATTGTCCTGCAGGAGCTGATCAAACAAGACGG
CAAACAAAGCGATTCAAGCGAATGGAAAAAACAGCTCGCAGAATGGAAAGAAGAGTATCCGCTCTGGTATGTAGATAATG
AAGAAGAAGGTTTTAAACCTCAGAAATTGATTGAATATATTCATCAATTTACAAAAGGAGAGGCCATTGTCGCAACGGAT
GTAGGCCAGCATCAAATGTGGTCAGCGCAATTTTATCCGTTCCAAAAAGCAGATAAATGGGTCACGTCAGGCGGACTTGG
AACGATGGGATTCGGTCTTCCGGCGGCGATCGGCGCACAGCTGGCCGAAAAAGATGCTACTGTTGTCGCGGTTGTCGGAG
ACGGCGGATTCCAAATGACGCTTCAAGAACTCGATGTTATTCGCGAATTAAATCTTCCGGTCAAGGTAGTGATTTTAAAT
AACGCTTGTCTCGGAATGGTCAGACAGTGGCAGGAAATTTTCTATGAAGAACGTTATTCAGAATCTAAATTCGCTTCTCA
GCCTGACTTCGTCAAATTGTCCGAAGCATACGGCATTAAAGGCATCAGAATTTCATCAGAAGCGGAAGCAAAGGAAAAGC
TGGAAGAGGCATTAACATCAAGAGAACCTGTTGTCATTGACGTGCGGGTTGCCAGCGAAGAAAAAGTATTCCCGATGGTG
GCTCCGGGGAAAGGGCTGCATGAAATGGTGGGGGTGAAACCTTGA

Upstream 100 bases:

>100_bases
GCTGATTGTGGGCGGAAGGGCTTTTTTTATTGAATAATCAGCTATCTAGCTAATGAAAAGATGATCTTTAAAGGATGAAA
ATCCAAAAGGAGGAACTAAA

Downstream 100 bases:

>100_bases
AAAGAATTATCACATTGACTGTGGTGAACCGCTCCGGGGTGTTAAACCGGATCACCGGTCTATTCACAAAAAGGCATTAC
AACATTGAAAGCATTACAGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MGTNVQVDSASAECTQTMSGALMLIESLKKEKVEMIFGYPGGAVLPIYDKLYNSGLVHILPRHEQGAIHAAEGYARVSGK
PGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFH
IATTGRPGPVLIDIPKDVATIEGEFSYDHEMNLPGYQPTTEPNYLQIRKLVEAVSSAKKPVILAGAGVLHGKASEELKNY
AEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALHECDLLISIGARFDDRVTGNLKHFARNAKIAHIDIDPAE
IGKIMKTQIPVVGDSKIVLQELIKQDGKQSDSSEWKKQLAEWKEEYPLWYVDNEEEGFKPQKLIEYIHQFTKGEAIVATD
VGQHQMWSAQFYPFQKADKWVTSGGLGTMGFGLPAAIGAQLAEKDATVVAVVGDGGFQMTLQELDVIRELNLPVKVVILN
NACLGMVRQWQEIFYEERYSESKFASQPDFVKLSEAYGIKGIRISSEAEAKEKLEEALTSREPVVIDVRVASEEKVFPMV
APGKGLHEMVGVKP

Sequences:

>Translated_574_residues
MGTNVQVDSASAECTQTMSGALMLIESLKKEKVEMIFGYPGGAVLPIYDKLYNSGLVHILPRHEQGAIHAAEGYARVSGK
PGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFH
IATTGRPGPVLIDIPKDVATIEGEFSYDHEMNLPGYQPTTEPNYLQIRKLVEAVSSAKKPVILAGAGVLHGKASEELKNY
AEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALHECDLLISIGARFDDRVTGNLKHFARNAKIAHIDIDPAE
IGKIMKTQIPVVGDSKIVLQELIKQDGKQSDSSEWKKQLAEWKEEYPLWYVDNEEEGFKPQKLIEYIHQFTKGEAIVATD
VGQHQMWSAQFYPFQKADKWVTSGGLGTMGFGLPAAIGAQLAEKDATVVAVVGDGGFQMTLQELDVIRELNLPVKVVILN
NACLGMVRQWQEIFYEERYSESKFASQPDFVKLSEAYGIKGIRISSEAEAKEKLEEALTSREPVVIDVRVASEEKVFPMV
APGKGLHEMVGVKP
>Mature_573_residues
GTNVQVDSASAECTQTMSGALMLIESLKKEKVEMIFGYPGGAVLPIYDKLYNSGLVHILPRHEQGAIHAAEGYARVSGKP
GVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVIGSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFHI
ATTGRPGPVLIDIPKDVATIEGEFSYDHEMNLPGYQPTTEPNYLQIRKLVEAVSSAKKPVILAGAGVLHGKASEELKNYA
EQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALHECDLLISIGARFDDRVTGNLKHFARNAKIAHIDIDPAEI
GKIMKTQIPVVGDSKIVLQELIKQDGKQSDSSEWKKQLAEWKEEYPLWYVDNEEEGFKPQKLIEYIHQFTKGEAIVATDV
GQHQMWSAQFYPFQKADKWVTSGGLGTMGFGLPAAIGAQLAEKDATVVAVVGDGGFQMTLQELDVIRELNLPVKVVILNN
ACLGMVRQWQEIFYEERYSESKFASQPDFVKLSEAYGIKGIRISSEAEAKEKLEEALTSREPVVIDVRVASEEKVFPMVA
PGKGLHEMVGVKP

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=25.3054101221641, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI21361361, Length=579, Percent_Identity=23.8341968911917, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=48.0565371024735, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=43.4397163120567, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1786717, Length=543, Percent_Identity=35.3591160220994, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=29.4117647058824, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788716, Length=547, Percent_Identity=24.8628884826325, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=27.5800711743772, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=27.5800711743772, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17542570, Length=568, Percent_Identity=24.8239436619718, Blast_Score=143, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=580, Percent_Identity=46.2068965517241, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6320816, Length=557, Percent_Identity=22.4416517055655, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=24.8979591836735, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320588, Length=559, Percent_Identity=21.6457960644007, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=490, Percent_Identity=24.4897959183673, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=492, Percent_Identity=23.5772357723577, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922626, Length=558, Percent_Identity=24.1935483870968, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVB_BACSU (P37251)

Other databases:

- EMBL:   L03181
- EMBL:   Z75208
- EMBL:   AL009126
- EMBL:   M87009
- PIR:   B69644
- RefSeq:   NP_390709.1
- ProteinModelPortal:   P37251
- SMR:   P37251
- EnsemblBacteria:   EBBACT00000000811
- GeneID:   936792
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28310
- NMPDR:   fig|224308.1.peg.2834
- GenoList:   BSU28310
- GeneTree:   EBGT00070000031807
- HOGENOM:   HBG323037
- OMA:   RLVFIDI
- PhylomeDB:   P37251
- ProtClustDB:   PRK07710
- BioCyc:   BSUB:BSU28310-MONOMER
- BRENDA:   2.2.1.6
- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00118

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 62590; Mature: 62458

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 64-64 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTNVQVDSASAECTQTMSGALMLIESLKKEKVEMIFGYPGGAVLPIYDKLYNSGLVHIL
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHCCCEEEEE
PRHEQGAIHAAEGYARVSGKPGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSV
ECCCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHH
IGSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFHIATTGRPGPVLIDIPKDVAT
HCCCHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEEEECCCCCEE
IEGEFSYDHEMNLPGYQPTTEPNYLQIRKLVEAVSSAKKPVILAGAGVLHGKASEELKNY
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHHHHH
AEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALHECDLLISIGARFDDRVTG
HHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCEEEECEEHHHCEEEEEECCCCCCCCCC
NLKHFARNAKIAHIDIDPAEIGKIMKTQIPVVGDSKIVLQELIKQDGKQSDSSEWKKQLA
CHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
EWKEEYPLWYVDNEEEGFKPQKLIEYIHQFTKGEAIVATDVGQHQMWSAQFYPFQKADKW
HHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCHHHCCC
VTSGGLGTMGFGLPAAIGAQLAEKDATVVAVVGDGGFQMTLQELDVIRELNLPVKVVILN
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHHHCCCCEEEEEEC
NACLGMVRQWQEIFYEERYSESKFASQPDFVKLSEAYGIKGIRISSEAEAKEKLEEALTS
CHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEHHHCCCCEEEECCHHHHHHHHHHHHHC
REPVVIDVRVASEEKVFPMVAPGKGLHEMVGVKP
CCCEEEEEEECCCCCCCEEECCCCCHHHHCCCCC
>Mature Secondary Structure 
GTNVQVDSASAECTQTMSGALMLIESLKKEKVEMIFGYPGGAVLPIYDKLYNSGLVHIL
CCCEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHCCCEEEEE
PRHEQGAIHAAEGYARVSGKPGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSV
ECCCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHH
IGSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFHIATTGRPGPVLIDIPKDVAT
HCCCHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEEEECCCCCEE
IEGEFSYDHEMNLPGYQPTTEPNYLQIRKLVEAVSSAKKPVILAGAGVLHGKASEELKNY
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHHHHH
AEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALHECDLLISIGARFDDRVTG
HHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCEEEECEEHHHCEEEEEECCCCCCCCCC
NLKHFARNAKIAHIDIDPAEIGKIMKTQIPVVGDSKIVLQELIKQDGKQSDSSEWKKQLA
CHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
EWKEEYPLWYVDNEEEGFKPQKLIEYIHQFTKGEAIVATDVGQHQMWSAQFYPFQKADKW
HHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCHHHCCC
VTSGGLGTMGFGLPAAIGAQLAEKDATVVAVVGDGGFQMTLQELDVIRELNLPVKVVILN
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHHHCCCCEEEEEEC
NACLGMVRQWQEIFYEERYSESKFASQPDFVKLSEAYGIKGIRISSEAEAKEKLEEALTS
CHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEHHHCCCCEEEECCHHHHHHHHHHHHHC
REPVVIDVRVASEEKVFPMVAPGKGLHEMVGVKP
CCCEEEEEEECCCCCCCEEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659