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The map label for this gene is tig

Identifier: 16079875

GI number: 16079875

Start: 2886315

End: 2887589

Strand: Reverse

Name: tig

Synonym: BSU28230

Alternate gene names: 16079875

Gene position: 2887589-2886315 (Counterclockwise)

Preceding gene: 255767649

Following gene: 255767648

Centisome position: 68.5

GC content: 41.41

Gene sequence:

>1275_bases
ATGTCTGTAAAATGGGAAAAACAAGAAGGCAACGAAGGCGTTTTAACGGTTGAAGTTGATGCTGAAACGTTTAAAACAGC
ACTTGATGATGCATTTAAAAAAGTAGTAAAACAAGTTTCAATTCCTGGATTCCGTAAAGGTAAAATTCCTCGCGGATTAT
TCGAACAGCGCTTTGGCGTAGAAGCTCTTTACCAAGATGCTTTGGATATTCTTCTTCCTGTAGAATACCCTAAAGCGGTT
GAAGAAGCTGGAATCGAGCCTGTAGACCGTCCTGAAATCGATGTTGAAAAAATCGAAAAAGGCGAAAGCTTAATCTTCAC
TGCGAAAGTAACAGTAAAACCTGAAGTGAAGCTTGGCGAATACAAAGGCCTGGGCATTGAAAAAGACGACACAACTGTAA
CTGACGAAGATGTTCAAAACGAACTAAAAGCCCTTCAAGAGCGTCAAGCTGAGCTTGTTGTAAAAGAAGAAGGCGCCGTT
GAAGAAGGAAACACAGTTGTTCTTGATTTCGAAGGATTCGTTGACGGCGAAGCTTTCGAAGGCGGAAAAGCTGAAAACTA
CTCTCTAGAAGTAGGTTCTGGTTCATTCATCCCTGGCTTCGAAGATCAATTAGTTGGCCTTGAAGCAGGTGCTGAGAAGG
ATGTTGAAGTGACATTCCCTGAGGAGTACCACGCTGAAGATCTTGCAGGCAAACCAGCTGTGTTTAAAGTGAAAATTCAC
GAAATCAAAGCGAAAGAGCTTCCTGAGCTTGACGATGAATTCGCAAAAGATATCGATGAAGAAGTGGAAACACTTGCTGA
ATTAACTGAAAAAACAAAGAAACGTTTAGAAGAAGCAAAAGAAAACGAAGCTGACGCTAAATTGCGTGAAGAGCTTGTTC
TGAAAGCTTCTGAAAATGCTGAAATCGATGTGCCTCAAGCAATGGTCGACACTGAGCTTGACCGCATGCTGAAAGAATTC
GAACAGCGTCTGCAAATGCAAGGCATGAACCTTGAGCTTTACACTCAATTCTCAGGCCAAGATGAAGCTGCGCTTAAAGA
GCAAATGAAAGAAGATGCTGAAAAACGCGTAAAATCTAACCTGACTCTTGAGGCGATTGCGAAAGCGGAAAACCTTGAAG
TATCAGATGAGGAAGTTGATGCTGAGCTTACAAAAATGGCTGAAGCATACAACATGCCTGTTGAAAATATTAAACAAGCC
ATCGGTTCTACTGACGCGATGAAAGAAGATTTAAAAGTTCGCAAAGCAATTGATTTTCTTGTAGAAAACCGTTAA

Upstream 100 bases:

>100_bases
ATTCTGATCTTTGGCAGCTTTGTTTTTGTTGGACGAAGCCGAATGATTGCTTTAGAATGGAAGACCGACACAACCTGAAT
AGATTTTTGGAGGGAAACAC

Downstream 100 bases:

>100_bases
TATGGTGCATAATAATAGTACTAATAAAACAGGGCGCGAATCATTCGTGCCTTGTTTTGTACAATTATGAGTTATCTCTT
TTTCTTGGGGCAATATAGTT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSVKWEKQEGNEGVLTVEVDAETFKTALDDAFKKVVKQVSIPGFRKGKIPRGLFEQRFGVEALYQDALDILLPVEYPKAV
EEAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGEYKGLGIEKDDTTVTDEDVQNELKALQERQAELVVKEEGAV
EEGNTVVLDFEGFVDGEAFEGGKAENYSLEVGSGSFIPGFEDQLVGLEAGAEKDVEVTFPEEYHAEDLAGKPAVFKVKIH
EIKAKELPELDDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENAEIDVPQAMVDTELDRMLKEF
EQRLQMQGMNLELYTQFSGQDEAALKEQMKEDAEKRVKSNLTLEAIAKAENLEVSDEEVDAELTKMAEAYNMPVENIKQA
IGSTDAMKEDLKVRKAIDFLVENR

Sequences:

>Translated_424_residues
MSVKWEKQEGNEGVLTVEVDAETFKTALDDAFKKVVKQVSIPGFRKGKIPRGLFEQRFGVEALYQDALDILLPVEYPKAV
EEAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGEYKGLGIEKDDTTVTDEDVQNELKALQERQAELVVKEEGAV
EEGNTVVLDFEGFVDGEAFEGGKAENYSLEVGSGSFIPGFEDQLVGLEAGAEKDVEVTFPEEYHAEDLAGKPAVFKVKIH
EIKAKELPELDDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENAEIDVPQAMVDTELDRMLKEF
EQRLQMQGMNLELYTQFSGQDEAALKEQMKEDAEKRVKSNLTLEAIAKAENLEVSDEEVDAELTKMAEAYNMPVENIKQA
IGSTDAMKEDLKVRKAIDFLVENR
>Mature_423_residues
SVKWEKQEGNEGVLTVEVDAETFKTALDDAFKKVVKQVSIPGFRKGKIPRGLFEQRFGVEALYQDALDILLPVEYPKAVE
EAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGEYKGLGIEKDDTTVTDEDVQNELKALQERQAELVVKEEGAVE
EGNTVVLDFEGFVDGEAFEGGKAENYSLEVGSGSFIPGFEDQLVGLEAGAEKDVEVTFPEEYHAEDLAGKPAVFKVKIHE
IKAKELPELDDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENAEIDVPQAMVDTELDRMLKEFE
QRLQMQGMNLELYTQFSGQDEAALKEQMKEDAEKRVKSNLTLEAIAKAENLEVSDEEVDAELTKMAEAYNMPVENIKQAI
GSTDAMKEDLKVRKAIDFLVENR

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=404, Percent_Identity=36.6336633663366, Blast_Score=212, Evalue=4e-56,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 47488; Mature: 47357

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKWEKQEGNEGVLTVEVDAETFKTALDDAFKKVVKQVSIPGFRKGKIPRGLFEQRFGV
CCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
EALYQDALDILLPVEYPKAVEEAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGE
HHHHHHHHHHEECCCCHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEEEECCCCEECC
YKGLGIEKDDTTVTDEDVQNELKALQERQAELVVKEEGAVEEGNTVVLDFEGFVDGEAFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKAENYSLEVGSGSFIPGFEDQLVGLEAGAEKDVEVTFPEEYHAEDLAGKPAVFKVKIH
CCCCCCEEEEECCCCCCCCCHHHEEECCCCCCCCCEEECCHHHCHHHCCCCCEEEEEEEE
EIKAKELPELDDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENA
ECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
EIDVPQAMVDTELDRMLKEFEQRLQMQGMNLELYTQFSGQDEAALKEQMKEDAEKRVKSN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHC
LTLEAIAKAENLEVSDEEVDAELTKMAEAYNMPVENIKQAIGSTDAMKEDLKVRKAIDFL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
VENR
HCCC
>Mature Secondary Structure 
SVKWEKQEGNEGVLTVEVDAETFKTALDDAFKKVVKQVSIPGFRKGKIPRGLFEQRFGV
CCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
EALYQDALDILLPVEYPKAVEEAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGE
HHHHHHHHHHEECCCCHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEEEECCCCEECC
YKGLGIEKDDTTVTDEDVQNELKALQERQAELVVKEEGAVEEGNTVVLDFEGFVDGEAFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKAENYSLEVGSGSFIPGFEDQLVGLEAGAEKDVEVTFPEEYHAEDLAGKPAVFKVKIH
CCCCCCEEEEECCCCCCCCCHHHEEECCCCCCCCCEEECCHHHCHHHCCCCCEEEEEEEE
EIKAKELPELDDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENA
ECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
EIDVPQAMVDTELDRMLKEFEQRLQMQGMNLELYTQFSGQDEAALKEQMKEDAEKRVKSN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHC
LTLEAIAKAENLEVSDEEVDAELTKMAEAYNMPVENIKQAIGSTDAMKEDLKVRKAIDFL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
VENR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA