Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 16079810

GI number: 16079810

Start: 2816535

End: 2817809

Strand: Reverse

Name: hisS

Synonym: BSU27560

Alternate gene names: 16079810

Gene position: 2817809-2816535 (Counterclockwise)

Preceding gene: 16079811

Following gene: 16079809

Centisome position: 66.84

GC content: 45.1

Gene sequence:

>1275_bases
ATGGGATATAACATTCCGAGAGGAACACAGGATATTCTGCCTGGAGAATCAGATCGCTGGCAGTTTGTTGAACAAATTAT
GAGAGACACTTGCCGCACTTATCAATATAAAGAAATCCGCACACCGATTTTTGAGCATACAGAACTGTTTGCCAGAGGCG
TCGGAGAATCAACGGATATCGTACAAAAAGAAATGTATACCTTCGAGGACCGCAAAGGCAGAAGCCTGACGCTTCGTCCG
GAGGGAACAGCTGCTGCGGTTCGCGCTTTTAATGAAAACAAGCTGTTCGCAAATCCTGTACAGCCGACAAAGCTTTATTA
TGTCGGGCCGATGTTCCGTTATGAACGTCCTCAGACGGGCCGTTACCGCCAGTTTTATCAGTTTGGAATTGAAGCGATTG
GTTCGAAAGACCCTGCAATCGACGCTGAAGTAATGGCGCTTGCGATGAGTATTTATGAAAAAGCAGGTTTAGAAAACGTT
AAGCTTGTCATAAACAGTCTCGGCGATCAAGATAGCCGCAAAAGCTACAGAGAAGCGCTCGTGAAACACTTCGAGCCTCG
CATCGAAGAATTTTGTTCAGACTGCCAGTCCCGGCTGCACACGAACCCGCTTAGAATTTTGGACTGCAAAAAAGACCGTG
ATCATGAGCTGATGAAATCGGCACCTTCGATTTTGACATATTTAAATGAAGAATCAGCCGCTTATTTTGAAAAAGTGAAA
CAATACTTAAATGATCTTGGCATTTCGTATGAAATCGATCCGAACCTTGTGAGAGGGCTGGATTATTACAACCACACCGC
ATTTGAAATTATGAGCAATGCGGAGGGCTTTGGCGCGATTACGACACTTGCCGGCGGCGGACGCTACGACGGGCTTGTCG
AACAGATCGGCGGACCTGAAGCACCGGGCATCGGATTTGCGATGAGCATCGAACGCTTGCTTGCCGCAATTGATGCTGAA
AAAAGAGAGCTGCCTGTTGATAAGGGAATCGACTGCTATATCGTCACACTCGGCGAAAAAGCAAAGGATTATTCTGTTTC
ATTGGTGTACAAACTGAGAGAAGCAGGCATTTCCAGTGAAATCGATTATGAAAATAAGAAAATGAAAGGCCAGTTTAAAA
CAGCTGATCGCTTAAAGGCCAGATTTATTGCGATTTTGGGTGAAGATGAGCTTGCCCAAAACAAAATCAATGTAAAGGAT
GCACAGACGGGCGAACAGATTGAAGTGGCACTTGATGAATTTATACATGTGATGAAGGCAAACCAAAAGGGATAA

Upstream 100 bases:

>100_bases
TCGTCCCTACTATCATGTATAGTAGGGGCGGGAGTTTTTTTCATTTTCATCATTAACGATCATTGGATATTTTATAAGGA
ATACATAAGGAGGTCTACCT

Downstream 100 bases:

>100_bases
GGAGTGAAACGCATTGTTTGGAAGAACATATTATTGCGGTGATATAACTGAAAAAGCAATTGGCGAATCTGTAACGCTGA
AAGGCTGGGTCCAAAAAAGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGYNIPRGTQDILPGESDRWQFVEQIMRDTCRTYQYKEIRTPIFEHTELFARGVGESTDIVQKEMYTFEDRKGRSLTLRP
EGTAAAVRAFNENKLFANPVQPTKLYYVGPMFRYERPQTGRYRQFYQFGIEAIGSKDPAIDAEVMALAMSIYEKAGLENV
KLVINSLGDQDSRKSYREALVKHFEPRIEEFCSDCQSRLHTNPLRILDCKKDRDHELMKSAPSILTYLNEESAAYFEKVK
QYLNDLGISYEIDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLVEQIGGPEAPGIGFAMSIERLLAAIDAE
KRELPVDKGIDCYIVTLGEKAKDYSVSLVYKLREAGISSEIDYENKKMKGQFKTADRLKARFIAILGEDELAQNKINVKD
AQTGEQIEVALDEFIHVMKANQKG

Sequences:

>Translated_424_residues
MGYNIPRGTQDILPGESDRWQFVEQIMRDTCRTYQYKEIRTPIFEHTELFARGVGESTDIVQKEMYTFEDRKGRSLTLRP
EGTAAAVRAFNENKLFANPVQPTKLYYVGPMFRYERPQTGRYRQFYQFGIEAIGSKDPAIDAEVMALAMSIYEKAGLENV
KLVINSLGDQDSRKSYREALVKHFEPRIEEFCSDCQSRLHTNPLRILDCKKDRDHELMKSAPSILTYLNEESAAYFEKVK
QYLNDLGISYEIDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLVEQIGGPEAPGIGFAMSIERLLAAIDAE
KRELPVDKGIDCYIVTLGEKAKDYSVSLVYKLREAGISSEIDYENKKMKGQFKTADRLKARFIAILGEDELAQNKINVKD
AQTGEQIEVALDEFIHVMKANQKG
>Mature_423_residues
GYNIPRGTQDILPGESDRWQFVEQIMRDTCRTYQYKEIRTPIFEHTELFARGVGESTDIVQKEMYTFEDRKGRSLTLRPE
GTAAAVRAFNENKLFANPVQPTKLYYVGPMFRYERPQTGRYRQFYQFGIEAIGSKDPAIDAEVMALAMSIYEKAGLENVK
LVINSLGDQDSRKSYREALVKHFEPRIEEFCSDCQSRLHTNPLRILDCKKDRDHELMKSAPSILTYLNEESAAYFEKVKQ
YLNDLGISYEIDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLVEQIGGPEAPGIGFAMSIERLLAAIDAEK
RELPVDKGIDCYIVTLGEKAKDYSVSLVYKLREAGISSEIDYENKKMKGQFKTADRLKARFIAILGEDELAQNKINVKDA
QTGEQIEVALDEFIHVMKANQKG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=456, Percent_Identity=24.780701754386, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI15029520, Length=459, Percent_Identity=24.1830065359477, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1788861, Length=417, Percent_Identity=45.5635491606715, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71993693, Length=458, Percent_Identity=24.235807860262, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=458, Percent_Identity=24.235807860262, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24643061, Length=478, Percent_Identity=22.8033472803347, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24643059, Length=497, Percent_Identity=21.9315895372233, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48132; Mature: 48001

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYNIPRGTQDILPGESDRWQFVEQIMRDTCRTYQYKEIRTPIFEHTELFARGVGESTDI
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHH
VQKEMYTFEDRKGRSLTLRPEGTAAAVRAFNENKLFANPVQPTKLYYVGPMFRYERPQTG
HHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCEEECCCCCCEEEEECCHHCCCCCCCC
RYRQFYQFGIEAIGSKDPAIDAEVMALAMSIYEKAGLENVKLVINSLGDQDSRKSYREAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
VKHFEPRIEEFCSDCQSRLHTNPLRILDCKKDRDHELMKSAPSILTYLNEESAAYFEKVK
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
QYLNDLGISYEIDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLVEQIGGPE
HHHHHCCCEEEECHHHHHHHHHHCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCC
APGIGFAMSIERLLAAIDAEKRELPVDKGIDCYIVTLGEKAKDYSVSLVYKLREAGISSE
CCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCC
IDYENKKMKGQFKTADRLKARFIAILGEDELAQNKINVKDAQTGEQIEVALDEFIHVMKA
CCCCCHHHCCCHHHHHHHHHHHHEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
NQKG
CCCC
>Mature Secondary Structure 
GYNIPRGTQDILPGESDRWQFVEQIMRDTCRTYQYKEIRTPIFEHTELFARGVGESTDI
CCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHH
VQKEMYTFEDRKGRSLTLRPEGTAAAVRAFNENKLFANPVQPTKLYYVGPMFRYERPQTG
HHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCEEECCCCCCEEEEECCHHCCCCCCCC
RYRQFYQFGIEAIGSKDPAIDAEVMALAMSIYEKAGLENVKLVINSLGDQDSRKSYREAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
VKHFEPRIEEFCSDCQSRLHTNPLRILDCKKDRDHELMKSAPSILTYLNEESAAYFEKVK
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
QYLNDLGISYEIDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLVEQIGGPE
HHHHHCCCEEEECHHHHHHHHHHCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCC
APGIGFAMSIERLLAAIDAEKRELPVDKGIDCYIVTLGEKAKDYSVSLVYKLREAGISSE
CCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCC
IDYENKKMKGQFKTADRLKARFIAILGEDELAQNKINVKDAQTGEQIEVALDEFIHVMKA
CCCCCHHHCCCHHHHHHHHHHHHEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
NQKG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA