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The map label for this gene is aspS

Identifier: 16079809

GI number: 16079809

Start: 2814743

End: 2816521

Strand: Reverse

Name: aspS

Synonym: BSU27550

Alternate gene names: 16079809

Gene position: 2816521-2814743 (Counterclockwise)

Preceding gene: 16079810

Following gene: 255767630

Centisome position: 66.81

GC content: 47.72

Gene sequence:

>1779_bases
TTGTTTGGAAGAACATATTATTGCGGTGATATAACTGAAAAAGCAATTGGCGAATCTGTAACGCTGAAAGGCTGGGTCCA
AAAAAGACGAGACCTCGGCGGATTGATTTTTATTGACTTGCGTGACCGTACGGGCATTGTTCAAGTCGTTTTTAACCCTG
ATGTGTCAAAAGAAGCGCTTGCTATTGCGGAAGGCATCAGAAATGAATACGTGCTTGATATTCAAGGAAAAGTGGTGGCG
CGTGAAGAGGGAACAGTTAATCCGAATTTGAAAACAGGCGCGATCGAAATACATGCTGATGGAGTGAACGTATTAAATGC
TGCAAAGACACCTCCATTTGCGATTTCTGATCAAGCTGAAGAAGTGTCAGAGGACGTTCGTTTAAAACACCGTTACTTAG
ATTTGCGCAGACCGGCTATGTTCCAGACGATGCAGCTGCGCCATAACGTGACGAAAGCTGTTCGCAGCTTTTTAGATGAA
AATGGGTTTCTTGATATTGAAACGCCGATTTTAACAGGAAGCACGCCTGAAGGCGCACGTGACTACTTGGTGCCGAGCCG
TGTGCACGAAGGTGAGTTCTACGCGCTGCCGCAGTCTCCGCAGCTATTTAAACAGCTTCTGATGGTATCAGGCATTGAAC
GATATTATCAAATTGCCCGCTGTTTCCGCGATGAAGACTTGCGTGCGGACCGTCAGCCTGAGTTTACGCAAATTGATATT
GAAATGTCCTTTATGAGCCAGGAAGACATTATGTCATTGGCTGAGGAGATGATGGCAAAGGTTATGCGTGAAACAAAAGG
TGAGGAACTTCAGCTTCCTCTTCCTCGAATGACGTATGACGAAGCAATGAACAAGTACGGGTCTGATAAACCGGATACGC
GTTTTGACATGCTTTTGACGGATGTGTCTGACATTGTAAAGGATACGGAATTTAAAGTGTTCTCATCAGCTGTAGCAAAC
GGCGGCGTGGTCAAAGCCATCAATGTAAAAGGCGGTGCCGGCGATTACTCCAGAAAAGACATCGACGCTCTTGGCGCTTT
TGCTGCAAACTACGGGGCAAAAGGGCTTGCCTGGGTGAAGGTTGAGGCAGACGGAGTGAAAGGCCCGATCGCTAAATTCT
TTGATGAAGAAAAGCAGTCTAAGCTGATTGAAGCACTTGATGCTGCTGAAGGTGATTTGCTGCTGTTCGGAGCGGATCAA
TTCGAAGTTGTGGCCGCATCACTCGGCGCCCTGCGCTTAAAGCTTGGGAAAGAACGCGGACTGATTGACGAAAAATTGTT
CAATTTCTTATGGGTCATCGACTGGCCGCTATTAGAGCATGATCCGGAAGAAGGCCGTTTTTACGCAGCGCACCATCCGT
TCACAATGCCTGTCCGAGAAGACCTTGAGCTGATTGAAACAGCGCCTGAAGACATGAAAGCGCAAGCTTATGACCTCGTC
TTAAACGGCTATGAGCTTGGCGGCGGTTCAATCCGTATTTTCGAAAAGGATATTCAGGAAAAAATGTTTGCGCTTCTCGG
CTTCTCACCAGAAGAAGCGGCTGAACAGTTCGGATTCCTGCTGGAAGCATTTGAATACGGCGCTCCTCCGCACGGCGGAA
TCGCACTCGGCTTAGACCGTCTCGTTATGCTTCTCGCTGGACGTACGAACCTGAGAGATACGATTGCATTCCCGAAAACG
GCAAGTGCAAGCTGCCTGATGACAGAAGCGCCAGGCGAAGTAAGCGACGCCCAGCTTGACGAGCTTCATCTTTCTATCAA
GAAAAAAGTTAAGAACTGA

Upstream 100 bases:

>100_bases
AATGTAAAGGATGCACAGACGGGCGAACAGATTGAAGTGGCACTTGATGAATTTATACATGTGATGAAGGCAAACCAAAA
GGGATAAGGAGTGAAACGCA

Downstream 100 bases:

>100_bases
ATTTGCAGTTCGATTCTTGAAAATCAAAATCGTCTGTGATATGATTTTGACAAGTTAAATAAAGTCCTGATGTGTTAGTT
GTACACCTAGTTTTGACCGA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MFGRTYYCGDITEKAIGESVTLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKEALAIAEGIRNEYVLDIQGKVVA
REEGTVNPNLKTGAIEIHADGVNVLNAAKTPPFAISDQAEEVSEDVRLKHRYLDLRRPAMFQTMQLRHNVTKAVRSFLDE
NGFLDIETPILTGSTPEGARDYLVPSRVHEGEFYALPQSPQLFKQLLMVSGIERYYQIARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMSLAEEMMAKVMRETKGEELQLPLPRMTYDEAMNKYGSDKPDTRFDMLLTDVSDIVKDTEFKVFSSAVAN
GGVVKAINVKGGAGDYSRKDIDALGAFAANYGAKGLAWVKVEADGVKGPIAKFFDEEKQSKLIEALDAAEGDLLLFGADQ
FEVVAASLGALRLKLGKERGLIDEKLFNFLWVIDWPLLEHDPEEGRFYAAHHPFTMPVREDLELIETAPEDMKAQAYDLV
LNGYELGGGSIRIFEKDIQEKMFALLGFSPEEAAEQFGFLLEAFEYGAPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKT
ASASCLMTEAPGEVSDAQLDELHLSIKKKVKN

Sequences:

>Translated_592_residues
MFGRTYYCGDITEKAIGESVTLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKEALAIAEGIRNEYVLDIQGKVVA
REEGTVNPNLKTGAIEIHADGVNVLNAAKTPPFAISDQAEEVSEDVRLKHRYLDLRRPAMFQTMQLRHNVTKAVRSFLDE
NGFLDIETPILTGSTPEGARDYLVPSRVHEGEFYALPQSPQLFKQLLMVSGIERYYQIARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMSLAEEMMAKVMRETKGEELQLPLPRMTYDEAMNKYGSDKPDTRFDMLLTDVSDIVKDTEFKVFSSAVAN
GGVVKAINVKGGAGDYSRKDIDALGAFAANYGAKGLAWVKVEADGVKGPIAKFFDEEKQSKLIEALDAAEGDLLLFGADQ
FEVVAASLGALRLKLGKERGLIDEKLFNFLWVIDWPLLEHDPEEGRFYAAHHPFTMPVREDLELIETAPEDMKAQAYDLV
LNGYELGGGSIRIFEKDIQEKMFALLGFSPEEAAEQFGFLLEAFEYGAPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKT
ASASCLMTEAPGEVSDAQLDELHLSIKKKVKN
>Mature_592_residues
MFGRTYYCGDITEKAIGESVTLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKEALAIAEGIRNEYVLDIQGKVVA
REEGTVNPNLKTGAIEIHADGVNVLNAAKTPPFAISDQAEEVSEDVRLKHRYLDLRRPAMFQTMQLRHNVTKAVRSFLDE
NGFLDIETPILTGSTPEGARDYLVPSRVHEGEFYALPQSPQLFKQLLMVSGIERYYQIARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMSLAEEMMAKVMRETKGEELQLPLPRMTYDEAMNKYGSDKPDTRFDMLLTDVSDIVKDTEFKVFSSAVAN
GGVVKAINVKGGAGDYSRKDIDALGAFAANYGAKGLAWVKVEADGVKGPIAKFFDEEKQSKLIEALDAAEGDLLLFGADQ
FEVVAASLGALRLKLGKERGLIDEKLFNFLWVIDWPLLEHDPEEGRFYAAHHPFTMPVREDLELIETAPEDMKAQAYDLV
LNGYELGGGSIRIFEKDIQEKMFALLGFSPEEAAEQFGFLLEAFEYGAPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKT
ASASCLMTEAPGEVSDAQLDELHLSIKKKVKN

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=597, Percent_Identity=43.0485762144054, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI5031815, Length=262, Percent_Identity=29.7709923664122, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI194272210, Length=262, Percent_Identity=29.7709923664122, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI45439306, Length=270, Percent_Identity=26.2962962962963, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI226491502, Length=258, Percent_Identity=24.4186046511628, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI4758762, Length=290, Percent_Identity=24.8275862068966, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1788173, Length=595, Percent_Identity=52.436974789916, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI1790571, Length=304, Percent_Identity=28.9473684210526, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789256, Length=302, Percent_Identity=25.8278145695364, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787161, Length=299, Percent_Identity=26.7558528428094, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=35.6666666666667, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=269, Percent_Identity=24.907063197026, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=274, Percent_Identity=24.8175182481752, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=266, Percent_Identity=24.812030075188, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=268, Percent_Identity=27.6119402985075, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325153, Length=630, Percent_Identity=32.5396825396825, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6321807, Length=264, Percent_Identity=26.5151515151515, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323011, Length=249, Percent_Identity=27.3092369477912, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=143, Percent_Identity=27.972027972028, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=150, Percent_Identity=28.6666666666667, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24584738, Length=596, Percent_Identity=40.9395973154362, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=29.8755186721992, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24640849, Length=265, Percent_Identity=27.5471698113208, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640851, Length=265, Percent_Identity=27.5471698113208, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921528, Length=280, Percent_Identity=27.1428571428571, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922416, Length=339, Percent_Identity=25.3687315634218, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_BACSU (O32038)

Other databases:

- EMBL:   AL009126
- PIR:   D69591
- RefSeq:   NP_390633.1
- ProteinModelPortal:   O32038
- SMR:   O32038
- EnsemblBacteria:   EBBACT00000001768
- GeneID:   937549
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27550
- NMPDR:   fig|224308.1.peg.2758
- GenoList:   BSU27550
- GeneTree:   EBGT00050000000858
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   O32038
- ProtClustDB:   PRK00476
- BioCyc:   BSUB:BSU27550-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 65976; Mature: 65976

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRTYYCGDITEKAIGESVTLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKEAL
CCCCEEEECCCHHHHCCCCEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHH
AIAEGIRNEYVLDIQGKVVAREEGTVNPNLKTGAIEIHADGVNVLNAAKTPPFAISDQAE
HHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCEECCCHH
EVSEDVRLKHRYLDLRRPAMFQTMQLRHNVTKAVRSFLDENGFLDIETPILTGSTPEGAR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCC
DYLVPSRVHEGEFYALPQSPQLFKQLLMVSGIERYYQIARCFRDEDLRADRQPEFTQIDI
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFMSQEDIMSLAEEMMAKVMRETKGEELQLPLPRMTYDEAMNKYGSDKPDTRFDMLLT
EEECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHH
DVSDIVKDTEFKVFSSAVANGGVVKAINVKGGAGDYSRKDIDALGAFAANYGAKGLAWVK
HHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
VEADGVKGPIAKFFDEEKQSKLIEALDAAEGDLLLFGADQFEVVAASLGALRLKLGKERG
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
LIDEKLFNFLWVIDWPLLEHDPEEGRFYAAHHPFTMPVREDLELIETAPEDMKAQAYDLV
CHHHHHHHHHHEEECHHHCCCCCCCCEEEECCCEECCHHHHHHHHHCCCHHHHHHHEEEE
LNGYELGGGSIRIFEKDIQEKMFALLGFSPEEAAEQFGFLLEAFEYGAPPHGGIALGLDR
EECEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LVMLLAGRTNLRDTIAFPKTASASCLMTEAPGEVSDAQLDELHLSIKKKVKN
HHHHHHCCCCCCHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGRTYYCGDITEKAIGESVTLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKEAL
CCCCEEEECCCHHHHCCCCEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHH
AIAEGIRNEYVLDIQGKVVAREEGTVNPNLKTGAIEIHADGVNVLNAAKTPPFAISDQAE
HHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCEECCCHH
EVSEDVRLKHRYLDLRRPAMFQTMQLRHNVTKAVRSFLDENGFLDIETPILTGSTPEGAR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCC
DYLVPSRVHEGEFYALPQSPQLFKQLLMVSGIERYYQIARCFRDEDLRADRQPEFTQIDI
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFMSQEDIMSLAEEMMAKVMRETKGEELQLPLPRMTYDEAMNKYGSDKPDTRFDMLLT
EEECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHH
DVSDIVKDTEFKVFSSAVANGGVVKAINVKGGAGDYSRKDIDALGAFAANYGAKGLAWVK
HHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
VEADGVKGPIAKFFDEEKQSKLIEALDAAEGDLLLFGADQFEVVAASLGALRLKLGKERG
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
LIDEKLFNFLWVIDWPLLEHDPEEGRFYAAHHPFTMPVREDLELIETAPEDMKAQAYDLV
CHHHHHHHHHHEEECHHHCCCCCCCCEEEECCCEECCHHHHHHHHHCCCHHHHHHHEEEE
LNGYELGGGSIRIFEKDIQEKMFALLGFSPEEAAEQFGFLLEAFEYGAPPHGGIALGLDR
EECEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LVMLLAGRTNLRDTIAFPKTASASCLMTEAPGEVSDAQLDELHLSIKKKVKN
HHHHHHCCCCCCHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377