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The map label for this gene is levD

Identifier: 16079761

GI number: 16079761

Start: 2762395

End: 2762835

Strand: Reverse

Name: levD

Synonym: BSU27070

Alternate gene names: 16079761

Gene position: 2762835-2762395 (Counterclockwise)

Preceding gene: 255767624

Following gene: 16079760

Centisome position: 65.54

GC content: 40.82

Gene sequence:

>441_bases
ATGATTTCAGTTATTATCAGCGGTCATGGAGATTTTCCCATAGCATTAAAAGAGTCTTCAGGGATGATATTCGGTGAAGA
AAATAACCTGATTGCAGTGCCGTTTTTCAAAGGGGAAGGAATACAAACGCTGCAAGAAAAATATCATCAGGCGCTCAAGG
ATATCCCGGAAGAACATGAAGTGCTTTTTTTAGTCGATATTTTTGGAGGGACGCCATATAACGCGGCAGCTTCCTTTATT
GCTGAGGATCAAAGAATGGATATGGCTGCCGGCGTAAACCTGCCGATTCTGCTGGAGGTATTAAGCCTGAGAGAACATCT
GGCTCTCAAAGATTTGCTGAACAACTTAAAAGCAATGAGTCAGCAAAGCTTTCAAGTGTGCAGTGAACATTTAGAAAAAG
TAAAAACAGCCAATCAAGATACAAGAGAGGATGAATTATGA

Upstream 100 bases:

>100_bases
TGCTATTGGCTGAAATAACAATGAAAACGCTTAACACAACTGTGTTGGCACGATCCTTGCATTATATATGGATGTACAAA
ACAGGAAAGGAGCAATAGAT

Downstream 100 bases:

>100_bases
TGAACATTGTGTTAGCAAGAATTGATGACCGCTTTATTCACGGCCAAATCCTGACAAGGTGGATAAAAGTCCATGCAGCC
GACCGAATCATCGTTGTCTC

Product: phosphotransferase system (PTS) fructose-specific enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Fru; PTS system fructose-specific EIIA component; p16

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MISVIISGHGDFPIALKESSGMIFGEENNLIAVPFFKGEGIQTLQEKYHQALKDIPEEHEVLFLVDIFGGTPYNAAASFI
AEDQRMDMAAGVNLPILLEVLSLREHLALKDLLNNLKAMSQQSFQVCSEHLEKVKTANQDTREDEL

Sequences:

>Translated_146_residues
MISVIISGHGDFPIALKESSGMIFGEENNLIAVPFFKGEGIQTLQEKYHQALKDIPEEHEVLFLVDIFGGTPYNAAASFI
AEDQRMDMAAGVNLPILLEVLSLREHLALKDLLNNLKAMSQQSFQVCSEHLEKVKTANQDTREDEL
>Mature_146_residues
MISVIISGHGDFPIALKESSGMIFGEENNLIAVPFFKGEGIQTLQEKYHQALKDIPEEHEVLFLVDIFGGTPYNAAASFI
AEDQRMDMAAGVNLPILLEVLSLREHLALKDLLNNLKAMSQQSFQVCSEHLEKVKTANQDTREDEL

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain

Homologues:

Organism=Escherichia coli, GI1788120, Length=104, Percent_Identity=37.5, Blast_Score=82, Evalue=1e-17,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PTFA_BACSU (P26379)

Other databases:

- EMBL:   X56098
- EMBL:   X92868
- EMBL:   AL009126
- PIR:   S11398
- RefSeq:   NP_390585.1
- ProteinModelPortal:   P26379
- SMR:   P26379
- EnsemblBacteria:   EBBACT00000001442
- GeneID:   936536
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27070
- NMPDR:   fig|224308.1.peg.2710
- GenoList:   BSU27070
- GeneTree:   EBGT00050000006012
- HOGENOM:   HBG734163
- OMA:   KESSGMI
- PhylomeDB:   P26379
- ProtClustDB:   CLSK237978
- BioCyc:   BSUB:BSU27070-MONOMER
- GO:   GO:0005737
- InterPro:   IPR004701
- InterPro:   IPR013789
- TIGRFAMs:   TIGR00824

Pfam domain/function: PF03610 EIIA-man; SSF53062 PTS_EIIA_fruc

EC number: 2.7.1.69

Molecular weight: Translated: 16257; Mature: 16257

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: ACT_SITE 9-9

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVIISGHGDFPIALKESSGMIFGEENNLIAVPFFKGEGIQTLQEKYHQALKDIPEEHE
CEEEEEECCCCCEEEEECCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
VLFLVDIFGGTPYNAAASFIAEDQRMDMAAGVNLPILLEVLSLREHLALKDLLNNLKAMS
EEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQSFQVCSEHLEKVKTANQDTREDEL
HHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MISVIISGHGDFPIALKESSGMIFGEENNLIAVPFFKGEGIQTLQEKYHQALKDIPEEHE
CEEEEEECCCCCEEEEECCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
VLFLVDIFGGTPYNAAASFIAEDQRMDMAAGVNLPILLEVLSLREHLALKDLLNNLKAMS
EEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQSFQVCSEHLEKVKTANQDTREDEL
HHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377