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The map label for this gene is yqiG

Identifier: 16079477

GI number: 16079477

Start: 2516440

End: 2517558

Strand: Direct

Name: yqiG

Synonym: BSU24210

Alternate gene names: 16079477

Gene position: 2516440-2517558 (Clockwise)

Preceding gene: 16079467

Following gene: 255767548

Centisome position: 59.69

GC content: 47.63

Gene sequence:

>1119_bases
ATGAATCCTAAGTATAAGCCACTTTTTGAACCATTTACGTTTAAAAGCGGCGTTACAATCAACAACCGGATCGCAGTAGC
ACCGATGACTCATTACGCTTCTAATGAAGACGGTACAATATCTGAAGCGGAGCTCGACTACATCATCCCCCGTTCAAAAG
AGATGGGAATGGTGATTACAGCCTGCGCAAATGTTACACCGGACGGAAAAGCATTCCCCGGGCAGCCGGCCATCCATGAC
GATTCCAACATTCCAGGTTTAAAAAAGTTAGCACAAGCCATTCAGGCACAAGGCGCTAAAGCTGTTGTACAAATTCATCA
CGGCGGTATTGAGTGCCCGTCTGAGCTCGTTCCTCAACAGGATGTTGTGGGGCCAAGTGACGTGTTTGATAACGGCAAAC
AAATTGCTCGCGCATTAACAGAAGAAGAAGTGGAAAACATTGTGAAGGCGTTTGGAGAAGCGACAAGACGCGCCATTGAA
GCCGGCTTTGACGGTGTCGAAATTCACGGTGCAAACGGCTACTTAATTCAGCAGTTTTATTCTCCGAAAACCAACCAGCG
CACGGATCGCTGGGGAGGAAGCGATGAAAAACGATTAGCCTTCCCGCTCGCTATTGTCGATGAAGTGAAAAAAGCCGCTT
CAGAACATGCGAAGGGTGCATTCTTAGTCGGCTACCGCCTGTCTCCGGAAGAACCTGAGACACCGGGATTGACAATGACT
GAAACTTATACGCTTGTTGATGCTTTAGGGGATAAAGAATTGGATTATCTTCATATCTCACTGATGGACGTGAACTCAAA
AGCGCGCCGCGGTGCAGATCCGACTCGCACACGCATGGACTTATTGAATGAACGTGTCGGAAACAAAGTGCCGCTGATCG
CCGTCGGTTCCATCCATTCCGCTGATGACGCGCTTGCCGTCATCGAAAACGGTATTCCACTGGTCGCTATGGGACGCGAA
ATTCTAGTTGACCCTAACTGGACGGTAAAAGTAAAAGAAGGCCGTGAAAAGCAAATCGAAACAGTGATCAAAGGCACAGA
TAAAGAAAAATATCATTTGCCTGAACCGCTATGGCAAGCAATTGTGAACACACAAGGCTGGGTGCCTTATAAAGATTAA

Upstream 100 bases:

>100_bases
CTCTGCACTCAAGATATTCAGTCGGTAAAGCAGATCAATTGCATTAATTTTTACATATAGGCTAGAATATGTGCGAATCC
AAACGAAAGAAGATGATCAA

Downstream 100 bases:

>100_bases
TGTGCAAAGACTGCCGAAACGATTCGGCAGTCTTTTTTCCCTTTATATAAATAGTTTGTGTACACATCTAAACAGCAAAA
GAAAGAGCTCCCCACCAACT

Product: NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNPKYKPLFEPFTFKSGVTINNRIAVAPMTHYASNEDGTISEAELDYIIPRSKEMGMVITACANVTPDGKAFPGQPAIHD
DSNIPGLKKLAQAIQAQGAKAVVQIHHGGIECPSELVPQQDVVGPSDVFDNGKQIARALTEEEVENIVKAFGEATRRAIE
AGFDGVEIHGANGYLIQQFYSPKTNQRTDRWGGSDEKRLAFPLAIVDEVKKAASEHAKGAFLVGYRLSPEEPETPGLTMT
ETYTLVDALGDKELDYLHISLMDVNSKARRGADPTRTRMDLLNERVGNKVPLIAVGSIHSADDALAVIENGIPLVAMGRE
ILVDPNWTVKVKEGREKQIETVIKGTDKEKYHLPEPLWQAIVNTQGWVPYKD

Sequences:

>Translated_372_residues
MNPKYKPLFEPFTFKSGVTINNRIAVAPMTHYASNEDGTISEAELDYIIPRSKEMGMVITACANVTPDGKAFPGQPAIHD
DSNIPGLKKLAQAIQAQGAKAVVQIHHGGIECPSELVPQQDVVGPSDVFDNGKQIARALTEEEVENIVKAFGEATRRAIE
AGFDGVEIHGANGYLIQQFYSPKTNQRTDRWGGSDEKRLAFPLAIVDEVKKAASEHAKGAFLVGYRLSPEEPETPGLTMT
ETYTLVDALGDKELDYLHISLMDVNSKARRGADPTRTRMDLLNERVGNKVPLIAVGSIHSADDALAVIENGIPLVAMGRE
ILVDPNWTVKVKEGREKQIETVIKGTDKEKYHLPEPLWQAIVNTQGWVPYKD
>Mature_372_residues
MNPKYKPLFEPFTFKSGVTINNRIAVAPMTHYASNEDGTISEAELDYIIPRSKEMGMVITACANVTPDGKAFPGQPAIHD
DSNIPGLKKLAQAIQAQGAKAVVQIHHGGIECPSELVPQQDVVGPSDVFDNGKQIARALTEEEVENIVKAFGEATRRAIE
AGFDGVEIHGANGYLIQQFYSPKTNQRTDRWGGSDEKRLAFPLAIVDEVKKAASEHAKGAFLVGYRLSPEEPETPGLTMT
ETYTLVDALGDKELDYLHISLMDVNSKARRGADPTRTRMDLLNERVGNKVPLIAVGSIHSADDALAVIENGIPLVAMGRE
ILVDPNWTVKVKEGREKQIETVIKGTDKEKYHLPEPLWQAIVNTQGWVPYKD

Specific function: Unknown

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family

Homologues:

Organism=Escherichia coli, GI1787939, Length=352, Percent_Identity=30.6818181818182, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789463, Length=353, Percent_Identity=28.328611898017, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17559804, Length=357, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17559802, Length=356, Percent_Identity=29.7752808988764, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17564188, Length=357, Percent_Identity=28.8515406162465, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI72001454, Length=347, Percent_Identity=29.3948126801153, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=354, Percent_Identity=27.1186440677966, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17540738, Length=276, Percent_Identity=29.7101449275362, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=351, Percent_Identity=24.7863247863248, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=237, Percent_Identity=30.8016877637131, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325086, Length=280, Percent_Identity=27.8571428571429, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQIG_BACSU (P54524)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   C69961
- RefSeq:   NP_390301.1
- ProteinModelPortal:   P54524
- SMR:   P54524
- EnsemblBacteria:   EBBACT00000004042
- GeneID:   938659
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24210
- NMPDR:   fig|224308.1.peg.2425
- GenoList:   BSU24210
- GeneTree:   EBGT00050000002219
- HOGENOM:   HBG583461
- OMA:   FLVGYRF
- PhylomeDB:   P54524
- ProtClustDB:   CLSK887547
- BioCyc:   BSUB:BSU24210-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001155
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00724 Oxidored_FMN

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40806; Mature: 40806

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKYKPLFEPFTFKSGVTINNRIAVAPMTHYASNEDGTISEAELDYIIPRSKEMGMVIT
CCCCCCCCCCCCCCCCCCEECCEEEEEEEHHCCCCCCCCCCCCCEEEECCCCCCCCEEEE
ACANVTPDGKAFPGQPAIHDDSNIPGLKKLAQAIQAQGAKAVVQIHHGGIECPSELVPQQ
EECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCC
DVVGPSDVFDNGKQIARALTEEEVENIVKAFGEATRRAIEAGFDGVEIHGANGYLIQQFY
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEHEEC
SPKTNQRTDRWGGSDEKRLAFPLAIVDEVKKAASEHAKGAFLVGYRLSPEEPETPGLTMT
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEE
ETYTLVDALGDKELDYLHISLMDVNSKARRGADPTRTRMDLLNERVGNKVPLIAVGSIHS
HHHHHHHHCCCCCCEEEEEEEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCC
ADDALAVIENGIPLVAMGREILVDPNWTVKVKEGREKQIETVIKGTDKEKYHLPEPLWQA
CCHHHHHHHCCCCEEEECCEEEECCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHH
IVNTQGWVPYKD
HHCCCCCCCCCC
>Mature Secondary Structure
MNPKYKPLFEPFTFKSGVTINNRIAVAPMTHYASNEDGTISEAELDYIIPRSKEMGMVIT
CCCCCCCCCCCCCCCCCCEECCEEEEEEEHHCCCCCCCCCCCCCEEEECCCCCCCCEEEE
ACANVTPDGKAFPGQPAIHDDSNIPGLKKLAQAIQAQGAKAVVQIHHGGIECPSELVPQQ
EECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCC
DVVGPSDVFDNGKQIARALTEEEVENIVKAFGEATRRAIEAGFDGVEIHGANGYLIQQFY
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEHEEC
SPKTNQRTDRWGGSDEKRLAFPLAIVDEVKKAASEHAKGAFLVGYRLSPEEPETPGLTMT
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEE
ETYTLVDALGDKELDYLHISLMDVNSKARRGADPTRTRMDLLNERVGNKVPLIAVGSIHS
HHHHHHHHCCCCCCEEEEEEEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCC
ADDALAVIENGIPLVAMGREILVDPNWTVKVKEGREKQIETVIKGTDKEKYHLPEPLWQA
CCHHHHHHHCCCCEEEECCEEEECCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHH
IVNTQGWVPYKD
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377