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The map label for this gene is bkdR

Identifier: 16079466

GI number: 16079466

Start: 2504789

End: 2506867

Strand: Reverse

Name: bkdR

Synonym: BSU24100

Alternate gene names: 16079466

Gene position: 2506867-2504789 (Counterclockwise)

Preceding gene: 16079468

Following gene: 255767528

Centisome position: 59.47

GC content: 45.17

Gene sequence:

>2079_bases
ATGCAAAAGGTACTGATAGTAGGTGCCGGCAAAAGGGGAACGGCGTTATTACATATTCTGATTAAAACAGCCATCATAGA
TATCATTGCCGTAGTTGACAAAGACCCCGAGGCGCCGGGATTGAAAGAAGCAGAACAGTATGGGATTGCCGTATCATCTG
ATTGGAAACCTTACATACAACAAAAACCGGATATCGTCATTCATACTACCGGCGATCAAGCCGTGTTTGACGAGCTCCTT
CAAAGAAAGCATGAAGATACCATTGTCATGCCGGGAAAAATGGCATACATCGTTTTTCAGCTGATGGAAGAAAAGCAGCA
CCTGATTCAAATGCTAAAAGAGCAAACCTATAAATATGACAGAATTTTTAATTCCACCCATGATGGAATGATTTTTATTG
ATATCAATGAAGAAATCATTCTCTTTAACCATATGGCCGAAAAAATGGTCGGAAAAAAACGTGAGGAAGTCATTGGGCGC
CCAATAAAAGAAGTGATTCCGAGCACTAAGATGCCGCGGATTTTAAAAACAAGAGTGCCTGAATACAACCAGAAGCAGCT
GTTAGGCGATCATTTGCAAATTGTGACCACAAGACTGCCGATTATTGATGAAGGCGGACGTCTGCTCGGGGCTCTTTGTG
TGTTTAAAGATATCACTGATGCGGTCGAGCTGGCAGAGGAAGTGACCAACTTAAAGCAGGTCCGTACGATGCTGGAGGCA
ATTATTCAATCCTCCGATGAGGCGATTTCTGTCGTTGATGAAAACGGCATCGGCCTGCTAATCAATAAAGCGTATACGAA
AATGACGGGGCTTTCTGAAAAAGAAGTCATCGGCAAGCCTGCAAACACTGATATTTCCGAAGGCGAAAGCATGCATTTAA
AGGTGCTTGAAACAAGACGTCCTGTCCGCGGTGTCAGAATGAAGGTCGGCCCGAATGAAAAAGAAGTGATCGTTAATGTA
GCACCGGTGATTGTGGACGGGATTTTGAAAGGCAGCGTCGGCGTGATTCACGATGTTTCCGAAATTAAAATGCTGACAGC
GGAGCTTAATCGCGCCAGACAAATTATTCGCACGCTTGAAGCGAAATACACGTTTGACGACATTATCGGCAAAAGCGAGC
AAATGCTGGTTGCGCTTGAGCAGGCGAAGCTCGGTGCGAAAACACCGGCAACCATCTTGCTGCGCGGGGAATCAGGAACA
GGAAAAGAACTGTTCGCCCATGCCATCCATAACGAAAGTGACCGGAAATACAATAAATTTATTCGCGTGAATTGTGCAGC
CCTTTCTGAAAACCTGCTCGAATCAGAATTGTTCGGCTATGAGGATGGTGCGTTTTCAGGAGCGAAGCGCGGCGGTAAAA
AGGGTTTATTTGAGGAAGCAAATAACGGCAGTATTTTCTTAGATGAAATCGGTGAGCTGACACAAAATATGCAGGCCAAA
TTGCTACGCGTCCTTCAAGAAAAGGAAATCGTCAGAGTGGGCGGCACGAAAGCGATCCCTGTCAACGTCAGAGTGATCGC
GGCGACAAATGTAAATATTGAAAAGGCGATGGCGGACGGAACATTCCGGGAAGACCTGTATTACCGGATCAATCGCTATC
CGATCTCCATCCCGCCTTTGCGGCAGCGGTTGGAGGACATTGAAGCATTGAGCGTCAGACTTATCCAAAAGATCAACCGG
GATTACGGCAGGAATGTAAAAGGGCTCTCACAGCAAGCCTTGCGCGCCTTATCTGCCTACCATTGGCCTGGGAATGTCCG
GGAGCTTGAAAATGTCCTTGGACGAGCCATGATTTTTCTGAATCCGCATATGGAATGGATTGAAAAAGATCACTTGCCTG
TATTCGAACTTGAGCAAAAAGAAAATGACACAGATCAGGGAACAGGTTTTGATTTTCCGGATATTGAAGGCGAAAAGCTG
TCTGTCGCAGTAGAAAAATTTGAAGCGCATTTGATCCAGCAAACGCTTGAAAAGCATCATTTTAACCGGACAAAAACAGC
AAAAGCTCTCGGTGTCAGCATTCGGAATTTATATTACAAAATGGATAAATATGGCCTTGCAAATGAAGGCATGCAATAA

Upstream 100 bases:

>100_bases
TATTTTGTCAAGAGAAGAAAACGTGGTAACATAGGGTTGTGCAAATTATTGCAACGGAATAAATAGGTGTGCAAATTTTT
TCATACAAAGGGGTATCGGG

Downstream 100 bases:

>100_bases
TTTGCAGAATAAACGCAAACATCTGCACGAATGTTTCGGTATACCTGGTATGACAGCACCCTTAAGAGCTGGCATGGAAC
TTGCATAATAAAAGGCGGAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MQKVLIVGAGKRGTALLHILIKTAIIDIIAVVDKDPEAPGLKEAEQYGIAVSSDWKPYIQQKPDIVIHTTGDQAVFDELL
QRKHEDTIVMPGKMAYIVFQLMEEKQHLIQMLKEQTYKYDRIFNSTHDGMIFIDINEEIILFNHMAEKMVGKKREEVIGR
PIKEVIPSTKMPRILKTRVPEYNQKQLLGDHLQIVTTRLPIIDEGGRLLGALCVFKDITDAVELAEEVTNLKQVRTMLEA
IIQSSDEAISVVDENGIGLLINKAYTKMTGLSEKEVIGKPANTDISEGESMHLKVLETRRPVRGVRMKVGPNEKEVIVNV
APVIVDGILKGSVGVIHDVSEIKMLTAELNRARQIIRTLEAKYTFDDIIGKSEQMLVALEQAKLGAKTPATILLRGESGT
GKELFAHAIHNESDRKYNKFIRVNCAALSENLLESELFGYEDGAFSGAKRGGKKGLFEEANNGSIFLDEIGELTQNMQAK
LLRVLQEKEIVRVGGTKAIPVNVRVIAATNVNIEKAMADGTFREDLYYRINRYPISIPPLRQRLEDIEALSVRLIQKINR
DYGRNVKGLSQQALRALSAYHWPGNVRELENVLGRAMIFLNPHMEWIEKDHLPVFELEQKENDTDQGTGFDFPDIEGEKL
SVAVEKFEAHLIQQTLEKHHFNRTKTAKALGVSIRNLYYKMDKYGLANEGMQ

Sequences:

>Translated_692_residues
MQKVLIVGAGKRGTALLHILIKTAIIDIIAVVDKDPEAPGLKEAEQYGIAVSSDWKPYIQQKPDIVIHTTGDQAVFDELL
QRKHEDTIVMPGKMAYIVFQLMEEKQHLIQMLKEQTYKYDRIFNSTHDGMIFIDINEEIILFNHMAEKMVGKKREEVIGR
PIKEVIPSTKMPRILKTRVPEYNQKQLLGDHLQIVTTRLPIIDEGGRLLGALCVFKDITDAVELAEEVTNLKQVRTMLEA
IIQSSDEAISVVDENGIGLLINKAYTKMTGLSEKEVIGKPANTDISEGESMHLKVLETRRPVRGVRMKVGPNEKEVIVNV
APVIVDGILKGSVGVIHDVSEIKMLTAELNRARQIIRTLEAKYTFDDIIGKSEQMLVALEQAKLGAKTPATILLRGESGT
GKELFAHAIHNESDRKYNKFIRVNCAALSENLLESELFGYEDGAFSGAKRGGKKGLFEEANNGSIFLDEIGELTQNMQAK
LLRVLQEKEIVRVGGTKAIPVNVRVIAATNVNIEKAMADGTFREDLYYRINRYPISIPPLRQRLEDIEALSVRLIQKINR
DYGRNVKGLSQQALRALSAYHWPGNVRELENVLGRAMIFLNPHMEWIEKDHLPVFELEQKENDTDQGTGFDFPDIEGEKL
SVAVEKFEAHLIQQTLEKHHFNRTKTAKALGVSIRNLYYKMDKYGLANEGMQ
>Mature_692_residues
MQKVLIVGAGKRGTALLHILIKTAIIDIIAVVDKDPEAPGLKEAEQYGIAVSSDWKPYIQQKPDIVIHTTGDQAVFDELL
QRKHEDTIVMPGKMAYIVFQLMEEKQHLIQMLKEQTYKYDRIFNSTHDGMIFIDINEEIILFNHMAEKMVGKKREEVIGR
PIKEVIPSTKMPRILKTRVPEYNQKQLLGDHLQIVTTRLPIIDEGGRLLGALCVFKDITDAVELAEEVTNLKQVRTMLEA
IIQSSDEAISVVDENGIGLLINKAYTKMTGLSEKEVIGKPANTDISEGESMHLKVLETRRPVRGVRMKVGPNEKEVIVNV
APVIVDGILKGSVGVIHDVSEIKMLTAELNRARQIIRTLEAKYTFDDIIGKSEQMLVALEQAKLGAKTPATILLRGESGT
GKELFAHAIHNESDRKYNKFIRVNCAALSENLLESELFGYEDGAFSGAKRGGKKGLFEEANNGSIFLDEIGELTQNMQAK
LLRVLQEKEIVRVGGTKAIPVNVRVIAATNVNIEKAMADGTFREDLYYRINRYPISIPPLRQRLEDIEALSVRLIQKINR
DYGRNVKGLSQQALRALSAYHWPGNVRELENVLGRAMIFLNPHMEWIEKDHLPVFELEQKENDTDQGTGFDFPDIEGEKL
SVAVEKFEAHLIQQTLEKHHFNRTKTAKALGVSIRNLYYKMDKYGLANEGMQ

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1788550, Length=336, Percent_Identity=42.8571428571429, Blast_Score=259, Evalue=5e-70,
Organism=Escherichia coli, GI1789233, Length=470, Percent_Identity=31.9148936170213, Blast_Score=245, Evalue=8e-66,
Organism=Escherichia coli, GI1789087, Length=235, Percent_Identity=51.063829787234, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI87082117, Length=267, Percent_Identity=46.8164794007491, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=38.8059701492537, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1790437, Length=317, Percent_Identity=42.5867507886435, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1788905, Length=226, Percent_Identity=48.6725663716814, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87082152, Length=325, Percent_Identity=37.8461538461538, Blast_Score=208, Evalue=1e-54,
Organism=Escherichia coli, GI1790299, Length=227, Percent_Identity=44.9339207048458, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1787583, Length=383, Percent_Identity=34.7258485639687, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=335, Percent_Identity=34.0298507462687, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87081858, Length=528, Percent_Identity=24.6212121212121, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=37.6106194690265, Blast_Score=137, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQIR_BACSU (P54529)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   H69961
- RefSeq:   NP_390290.1
- ProteinModelPortal:   P54529
- EnsemblBacteria:   EBBACT00000002295
- GeneID:   938666
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24100
- NMPDR:   fig|224308.1.peg.2414
- GenoList:   BSU24100
- GeneTree:   EBGT00070000031840
- HOGENOM:   HBG740884
- OMA:   GITHRTV
- PhylomeDB:   P54529
- ProtClustDB:   CLSK873418
- BioCyc:   BSUB:BSU24100-MONOMER
- GO:   GO:0005622
- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR016040
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078
- Gene3D:   G3DSA:1.10.10.60
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00086
- SMART:   SM00091
- TIGRFAMs:   TIGR00229

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 77931; Mature: 77931

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50112 PAS; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xfe0d09c)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVLIVGAGKRGTALLHILIKTAIIDIIAVVDKDPEAPGLKEAEQYGIAVSSDWKPYIQ
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEECCCCCHHHC
QKPDIVIHTTGDQAVFDELLQRKHEDTIVMPGKMAYIVFQLMEEKQHLIQMLKEQTYKYD
CCCCEEEEECCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIFNSTHDGMIFIDINEEIILFNHMAEKMVGKKREEVIGRPIKEVIPSTKMPRILKTRVP
HHHCCCCCCEEEEEECCCEEEHHHHHHHHHCCHHHHHHCCCHHHHCCCCCCCHHHHHCCC
EYNQKQLLGDHLQIVTTRLPIIDEGGRLLGALCVFKDITDAVELAEEVTNLKQVRTMLEA
CCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIQSSDEAISVVDENGIGLLINKAYTKMTGLSEKEVIGKPANTDISEGESMHLKVLETRR
HHCCCCCEEEEEECCCCEEEEEHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCC
PVRGVRMKVGPNEKEVIVNVAPVIVDGILKGSVGVIHDVSEIKMLTAELNRARQIIRTLE
CCCCEEEEECCCCCEEEEEEHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
AKYTFDDIIGKSEQMLVALEQAKLGAKTPATILLRGESGTGKELFAHAIHNESDRKYNKF
HCCCHHHHCCCCHHHHEEEHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHE
IRVNCAALSENLLESELFGYEDGAFSGAKRGGKKGLFEEANNGSIFLDEIGELTQNMQAK
EEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHH
LLRVLQEKEIVRVGGTKAIPVNVRVIAATNVNIEKAMADGTFREDLYYRINRYPISIPPL
HHHHHHHCCCEEECCCEEEEEEEEEEEEECCCHHHHHCCCCHHHHHHHEEECCCCCCCHH
RQRLEDIEALSVRLIQKINRDYGRNVKGLSQQALRALSAYHWPGNVRELENVLGRAMIFL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEE
NPHMEWIEKDHLPVFELEQKENDTDQGTGFDFPDIEGEKLSVAVEKFEAHLIQQTLEKHH
CCCHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
FNRTKTAKALGVSIRNLYYKMDKYGLANEGMQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure
MQKVLIVGAGKRGTALLHILIKTAIIDIIAVVDKDPEAPGLKEAEQYGIAVSSDWKPYIQ
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEECCCCCHHHC
QKPDIVIHTTGDQAVFDELLQRKHEDTIVMPGKMAYIVFQLMEEKQHLIQMLKEQTYKYD
CCCCEEEEECCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIFNSTHDGMIFIDINEEIILFNHMAEKMVGKKREEVIGRPIKEVIPSTKMPRILKTRVP
HHHCCCCCCEEEEEECCCEEEHHHHHHHHHCCHHHHHHCCCHHHHCCCCCCCHHHHHCCC
EYNQKQLLGDHLQIVTTRLPIIDEGGRLLGALCVFKDITDAVELAEEVTNLKQVRTMLEA
CCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIQSSDEAISVVDENGIGLLINKAYTKMTGLSEKEVIGKPANTDISEGESMHLKVLETRR
HHCCCCCEEEEEECCCCEEEEEHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCC
PVRGVRMKVGPNEKEVIVNVAPVIVDGILKGSVGVIHDVSEIKMLTAELNRARQIIRTLE
CCCCEEEEECCCCCEEEEEEHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
AKYTFDDIIGKSEQMLVALEQAKLGAKTPATILLRGESGTGKELFAHAIHNESDRKYNKF
HCCCHHHHCCCCHHHHEEEHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHE
IRVNCAALSENLLESELFGYEDGAFSGAKRGGKKGLFEEANNGSIFLDEIGELTQNMQAK
EEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHH
LLRVLQEKEIVRVGGTKAIPVNVRVIAATNVNIEKAMADGTFREDLYYRINRYPISIPPL
HHHHHHHCCCEEECCCEEEEEEEEEEEEECCCHHHHHCCCCHHHHHHHEEECCCCCCCHH
RQRLEDIEALSVRLIQKINRDYGRNVKGLSQQALRALSAYHWPGNVRELENVLGRAMIFL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEE
NPHMEWIEKDHLPVFELEQKENDTDQGTGFDFPDIEGEKLSVAVEKFEAHLIQQTLEKHH
CCCHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
FNRTKTAKALGVSIRNLYYKMDKYGLANEGMQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377