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The map label for this gene is yqjW

Identifier: 16079428

GI number: 16079428

Start: 2464562

End: 2465800

Strand: Reverse

Name: yqjW

Synonym: BSU23710

Alternate gene names: 16079428

Gene position: 2465800-2464562 (Counterclockwise)

Preceding gene: 16079432

Following gene: 16079427

Centisome position: 58.49

GC content: 50.28

Gene sequence:

>1239_bases
ATGATGAAAGAAAAAGTGATTTTTCTCGTTGACATGCAATCGTTTTATGCATCTGTAGAGAAAGCGGAAAATCCACATTT
GAAAAATAGGCCCGTCATTGTTTCGGGTGACCCTGAAAAAAGGGGCGGAGTCGTATTGGCTGCCTGCCCGCTGGCGAAAC
AAAAGGGTGTGGTGAATGCTTCACGGCTGTGGGAGGCGCAGGAAAAGTGTCCTGAGGCTGTTGTGCTCCGGCCGCGTATG
CAGCGGTATATTGATGTATCACTGCAAATTACGGCCATTCTCGAGGAGTATACAGACCTTGTGGAGCCGTATTCCATCGA
TGAACAGTTCATGGACATTACAGGCAGCCAGAAGCTGTTTGGGACGCCGATGGAGATCGCGAAAAGCATTCAGGGCAGAA
TCATGCGGGAGATCGGCGTTTATGCACGGGTCGGAATCGGCCCTAACAAAGCGCTGGCCAAAATTGCGTGTGACAATTTT
GCCAAAAAGAATAAGAACGGTATTTTTACCTTAACGAAAGAAAATATGAAAACCGAAATGTGGCCGCTCCCGGTGGGCAG
CATGTTTGGCGTCGGGAGCCGCATGAAGCATCATTTAAATCGAATGGGCATCAGCACGATCGGCGGGCTCGCGGCTTTTC
CGCTCGATCTTTTAAAAAAGAAATGGGGCATTAACGGCCACGTGCTGTGGATGACGGCAAACGGAATCGACTATTCCCCT
GTGTCAACTTCGTCTCTGGACGGGCAAAAGGCGATAGGTCATGGAATGACTCTCCCGAGAGACTACGAACACTTTGACAA
AGAAATCAAGGTCGTATTGCTTGAGCTGAGTGAAGAGGTGTGCAGGCGAAGCCGAAACGCCGGGGTCATGGGGCAGACAG
TGTCAGTGAGCTGCCGGGGTGCTGATTTTGATTGGCCGACGGGCTTCAACCGGCAAGTGAAGCTGGCAGAGCCGACTAAT
TCTACGCAGGATGTATATGAGGCTGTACGACGGCTGTTTCTTACATTTTGGGACGGGAAACCCGTCCGCCGCCTCGGTGT
CAATCTGTCTCAGCTCTCATCTGATGACATATGGCAGCTCAATTTATTTCAGGATTATGCAAAGAAAATGAGCCTAGGCT
ATGTGATGGATGGCATTAAAAATCGATTCGGCGATACAGCAATCATCAGGGCGGCGTCACTGACAGCGGCAGGCCAGGCA
TTTGAACGTGCGGCTAAAATAGGGGGGCATTATAAATGA

Upstream 100 bases:

>100_bases
GGATCATATTTTCTGTCAGACAAGAATCATTTACCAGTATAAAACGAACATACTTTCGCATATAATGAAAAGCGAACATA
AGTTCTTTTTAGAGGTGTGT

Downstream 100 bases:

>100_bases
ACGACCATTTAAAAAGGGGCAACCTGCTGTGGGAAGGGAGCAGGATGTTTCTGCCGGAACATAAGCAAAGCCTGCTGGAG
AGAAAGCGACTTAAACAGAA

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV 2

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MMKEKVIFLVDMQSFYASVEKAENPHLKNRPVIVSGDPEKRGGVVLAACPLAKQKGVVNASRLWEAQEKCPEAVVLRPRM
QRYIDVSLQITAILEEYTDLVEPYSIDEQFMDITGSQKLFGTPMEIAKSIQGRIMREIGVYARVGIGPNKALAKIACDNF
AKKNKNGIFTLTKENMKTEMWPLPVGSMFGVGSRMKHHLNRMGISTIGGLAAFPLDLLKKKWGINGHVLWMTANGIDYSP
VSTSSLDGQKAIGHGMTLPRDYEHFDKEIKVVLLELSEEVCRRSRNAGVMGQTVSVSCRGADFDWPTGFNRQVKLAEPTN
STQDVYEAVRRLFLTFWDGKPVRRLGVNLSQLSSDDIWQLNLFQDYAKKMSLGYVMDGIKNRFGDTAIIRAASLTAAGQA
FERAAKIGGHYK

Sequences:

>Translated_412_residues
MMKEKVIFLVDMQSFYASVEKAENPHLKNRPVIVSGDPEKRGGVVLAACPLAKQKGVVNASRLWEAQEKCPEAVVLRPRM
QRYIDVSLQITAILEEYTDLVEPYSIDEQFMDITGSQKLFGTPMEIAKSIQGRIMREIGVYARVGIGPNKALAKIACDNF
AKKNKNGIFTLTKENMKTEMWPLPVGSMFGVGSRMKHHLNRMGISTIGGLAAFPLDLLKKKWGINGHVLWMTANGIDYSP
VSTSSLDGQKAIGHGMTLPRDYEHFDKEIKVVLLELSEEVCRRSRNAGVMGQTVSVSCRGADFDWPTGFNRQVKLAEPTN
STQDVYEAVRRLFLTFWDGKPVRRLGVNLSQLSSDDIWQLNLFQDYAKKMSLGYVMDGIKNRFGDTAIIRAASLTAAGQA
FERAAKIGGHYK
>Mature_412_residues
MMKEKVIFLVDMQSFYASVEKAENPHLKNRPVIVSGDPEKRGGVVLAACPLAKQKGVVNASRLWEAQEKCPEAVVLRPRM
QRYIDVSLQITAILEEYTDLVEPYSIDEQFMDITGSQKLFGTPMEIAKSIQGRIMREIGVYARVGIGPNKALAKIACDNF
AKKNKNGIFTLTKENMKTEMWPLPVGSMFGVGSRMKHHLNRMGISTIGGLAAFPLDLLKKKWGINGHVLWMTANGIDYSP
VSTSSLDGQKAIGHGMTLPRDYEHFDKEIKVVLLELSEEVCRRSRNAGVMGQTVSVSCRGADFDWPTGFNRQVKLAEPTN
STQDVYEAVRRLFLTFWDGKPVRRLGVNLSQLSSDDIWQLNLFQDYAKKMSLGYVMDGIKNRFGDTAIIRAASLTAAGQA
FERAAKIGGHYK

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain

Homologues:

Organism=Homo sapiens, GI7706681, Length=329, Percent_Identity=28.2674772036474, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI84043967, Length=329, Percent_Identity=28.2674772036474, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI154350220, Length=283, Percent_Identity=25.4416961130742, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI5729982, Length=354, Percent_Identity=23.728813559322, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1786425, Length=277, Percent_Identity=33.5740072202166, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1787432, Length=415, Percent_Identity=26.9879518072289, Blast_Score=108, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21355641, Length=256, Percent_Identity=25, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24644984, Length=256, Percent_Identity=25, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19923006, Length=255, Percent_Identity=25.8823529411765, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24668444, Length=118, Percent_Identity=34.7457627118644, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO42_BACSU (P54560)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   G69965
- RefSeq:   NP_390252.1
- ProteinModelPortal:   P54560
- SMR:   P54560
- EnsemblBacteria:   EBBACT00000000959
- GeneID:   938705
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23710
- NMPDR:   fig|224308.1.peg.2376
- GenoList:   BSU23710
- GeneTree:   EBGT00050000001510
- HOGENOM:   HBG734504
- OMA:   LAKMACD
- PhylomeDB:   P54560
- ProtClustDB:   PRK03103
- BioCyc:   BSUB:BSU23710-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- HAMAP:   MF_01113
- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880
- Gene3D:   G3DSA:3.30.1490.100
- PANTHER:   PTHR11076

Pfam domain/function: PF00817 IMS; SSF100879 DNA_pol_Y-fam_little_finger

EC number: =2.7.7.7

Molecular weight: Translated: 45945; Mature: 45945

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50173 UMUC

Important sites: ACT_SITE 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKEKVIFLVDMQSFYASVEKAENPHLKNRPVIVSGDPEKRGGVVLAACPLAKQKGVVNA
CCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEECCCHHHCCCCCH
SRLWEAQEKCPEAVVLRPRMQRYIDVSLQITAILEEYTDLVEPYSIDEQFMDITGSQKLF
HHHHHHHHHCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
GTPMEIAKSIQGRIMREIGVYARVGIGPNKALAKIACDNFAKKNKNGIFTLTKENMKTEM
CCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEE
WPLPVGSMFGVGSRMKHHLNRMGISTIGGLAAFPLDLLKKKWGINGHVLWMTANGIDYSP
CCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
VSTSSLDGQKAIGHGMTLPRDYEHFDKEIKVVLLELSEEVCRRSRNAGVMGQTVSVSCRG
CCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
ADFDWPTGFNRQVKLAEPTNSTQDVYEAVRRLFLTFWDGKPVRRLGVNLSQLSSDDIWQL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCCCCEEE
NLFQDYAKKMSLGYVMDGIKNRFGDTAIIRAASLTAAGQAFERAAKIGGHYK
HHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMKEKVIFLVDMQSFYASVEKAENPHLKNRPVIVSGDPEKRGGVVLAACPLAKQKGVVNA
CCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEECCCHHHCCCCCH
SRLWEAQEKCPEAVVLRPRMQRYIDVSLQITAILEEYTDLVEPYSIDEQFMDITGSQKLF
HHHHHHHHHCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
GTPMEIAKSIQGRIMREIGVYARVGIGPNKALAKIACDNFAKKNKNGIFTLTKENMKTEM
CCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEE
WPLPVGSMFGVGSRMKHHLNRMGISTIGGLAAFPLDLLKKKWGINGHVLWMTANGIDYSP
CCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
VSTSSLDGQKAIGHGMTLPRDYEHFDKEIKVVLLELSEEVCRRSRNAGVMGQTVSVSCRG
CCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
ADFDWPTGFNRQVKLAEPTNSTQDVYEAVRRLFLTFWDGKPVRRLGVNLSQLSSDDIWQL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCCCCEEE
NLFQDYAKKMSLGYVMDGIKNRFGDTAIIRAASLTAAGQAFERAAKIGGHYK
HHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377