Click here to switch to the map view.

The map label for this gene is nudF

Identifier: 16079418

GI number: 16079418

Start: 2458509

End: 2459066

Strand: Reverse

Name: nudF

Synonym: BSU23610

Alternate gene names: 16079418

Gene position: 2459066-2458509 (Counterclockwise)

Preceding gene: 16079420

Following gene: 255767513

Centisome position: 58.33

GC content: 43.73

Gene sequence:

>558_bases
ATGAAATCATTAGAAGAAAAAACAATTGCCAAAGAACAGATTTTTTCGGGTAAAGTCATTGATCTTTATGTCGAGGATGT
AGAGCTGCCAAACGGCAAAGCCAGTAAACGTGAAATTGTGAAACACCCTGGAGCTGTAGCGGTACTAGCCGTCACAGATG
AAGGGAAAATCATCATGGTCAAACAATTCCGTAAGCCGCTTGAGCGGACGATCGTTGAAATTCCGGCCGGTAAGCTTGAA
AAAGGTGAGGAGCCGGAGTATACGGCACTTCGGGAACTTGAAGAGGAAACCGGTTATACAGCAAAAAAACTGACAAAAAT
AACTGCGTTTTATACATCACCCGGATTTGCAGATGAAATCGTTCACGTTTTTCTTGCTGAGGAGCTTTCTGTGCTTGAAG
AAAAACGGGAGCTTGATGAGGACGAGTTTGTTGAAGTGATGGAGGTGACGCTTGAAGATGCGCTAAAGCTGGTTGAATCG
CGTGAAGTATATGATGCTAAAACAGCCTACGCGATTCAGTATCTTCAGCTGAAAGAAGCGCTCCAAGCACAAAAATGA

Upstream 100 bases:

>100_bases
CCTTTTGGCATGCGGTGCACTTTCACGAAGAAGCCCCCTTTTGCTCTAGTATGATAAAATGTATGGGGCATCATATAGAG
ACAGAACGGGAGATGAAGAA

Downstream 100 bases:

>100_bases
AAAAGATTTACGCGGATTTGCACATTCATATCGGCCGGACCTTTACAGGAAGGGCTGTCAAAATAACAGGCGCCAAAACA
TTAACGCTGGACAGAATTTT

Product: ADP-ribose pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEGKIIMVKQFRKPLERTIVEIPAGKLE
KGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVES
REVYDAKTAYAIQYLQLKEALQAQK

Sequences:

>Translated_185_residues
MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEGKIIMVKQFRKPLERTIVEIPAGKLE
KGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVES
REVYDAKTAYAIQYLQLKEALQAQK
>Mature_185_residues
MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEGKIIMVKQFRKPLERTIVEIPAGKLE
KGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVES
REVYDAKTAYAIQYLQLKEALQAQK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Homo sapiens, GI37594464, Length=167, Percent_Identity=32.3353293413174, Blast_Score=74, Evalue=8e-14,
Organism=Escherichia coli, GI1788810, Length=185, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1789412, Length=184, Percent_Identity=31.5217391304348, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319587, Length=139, Percent_Identity=33.0935251798561, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADPP_BACSU (P54570)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A69967
- RefSeq:   NP_390242.1
- ProteinModelPortal:   P54570
- SMR:   P54570
- EnsemblBacteria:   EBBACT00000001373
- GeneID:   938719
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23610
- NMPDR:   fig|224308.1.peg.2366
- GenoList:   BSU23610
- GeneTree:   EBGT00050000000491
- HOGENOM:   HBG692398
- OMA:   SANKWDE
- PhylomeDB:   P54570
- ProtClustDB:   CLSK887524
- BioCyc:   BSUB:BSU23610-MONOMER
- BRENDA:   3.6.1.13
- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR00502
- TIGRFAMs:   TIGR00052

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: =3.6.1.13

Molecular weight: Translated: 20980; Mature: 20980

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX; PS00287 CYSTATIN

Important sites: ACT_SITE 140-140 BINDING 64-64 BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEGKIIMV
CCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCEEEH
KQFRKPLERTIVEIPAGKLEKGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEI
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHEECCCCHHHHH
VHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVESREVYDAKTAYAIQYLQLKEA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAQK
HHCCC
>Mature Secondary Structure
MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEGKIIMV
CCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCEEEH
KQFRKPLERTIVEIPAGKLEKGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEI
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHEECCCCHHHHH
VHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVESREVYDAKTAYAIQYLQLKEA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAQK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272