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The map label for this gene is gpsA

Identifier: 16079340

GI number: 16079340

Start: 2389151

End: 2390188

Strand: Reverse

Name: gpsA

Synonym: BSU22830

Alternate gene names: 16079340

Gene position: 2390188-2389151 (Counterclockwise)

Preceding gene: 16079341

Following gene: 16079339

Centisome position: 56.7

GC content: 44.61

Gene sequence:

>1038_bases
ATGAAAAAAGTCACAATGCTTGGAGCGGGGAGTTGGGGAACAGCACTGGCTTTAGTTCTAACTGATAATGGAAATGAAGT
GTGTGTGTGGGCTCACCGTGCAGATTTAATTCATCAAATTAATGAGTTGCATGAAAACAAAGATTATTTGCCGAATGTTA
AGCTGTCTACATCCATTAAAGGAACAACAGATATGAAAGAGGCTGTTTCAGACGCAGATGTCATTATCGTTGCGGTCCCG
ACAAAAGCAATTCGGGAAGTGCTGAGACAGGCTGTCCCTTTTATAACGAAAAAGGCAGTCTTTGTTCATGTCAGCAAGGG
TATTGAGCCAGATTCACTTCTGCGCATTTCTGAAATTATGGAAATTGAGCTCCCGAGTGATGTCAGAAAAGATATCGTTG
TCCTTTCCGGCCCGAGTCATGCGGAAGAAGTAGGGCTGCGGCACCCCACAACTGTTACTGCATCTTCAAAGAGCATGAGG
GCAGCAGAAGAGGTGCAGGATCTATTTATTAATCACAATTTTCGGGTGTACACAAATCCTGACATTATCGGAGTTGAAAT
CGGAGGGGCTTTAAAAAATATTATTGCCCTTGCTGCAGGAATTACAGATGGTTTAGGGTACGGTGACAATGCCAAAGCTG
CTTTGATTACACGCGGACTTGCCGAAATCGCGAGACTCGGAACGAAAATGGGCGGAAATCCCTTGACGTTCTCTGGATTG
ACAGGAGTAGGCGATCTGATTGTGACGTGCACAAGTGTTCATTCCAGAAACTGGCGTGCGGGCAATTTGCTCGGAAAAGG
GTACAAGCTTGAAGATGTTCTTGAAGAGATGGGAATGGTAGTCGAAGGCGTCCGCACGACGAAAGCGGCTTATCAGCTTT
CGAAGAAATATGATGTTAAAATGCCGATTACAGAAGCTCTCCATCAGGTCCTATTCAACGGACAAAAAGTGGAAACCGCT
GTTGAATCTTTAATGGCGAGAGGGAAAACCCACGAGATGGAGGATTTGGTAAATACGTTTGAAAATCAAGTGAAGTAA

Upstream 100 bases:

>100_bases
AACGGTTTTTAGAAAACCGAATCAGAGACGCGTTCGGTTTTGAGGGGACACCAATCAAAATATTTGCAAGAGCTAGAAAA
TAAAAAGGTGTGAATCAAAC

Downstream 100 bases:

>100_bases
AAGTGTCAATCAAATGGTGAATCGCATATCTTAATTGAACGAAAGCCCGAAAAACAGAAGAACACCCAAGTCTTGGGATC
TCCTGAAACATTTTGCCGAT

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent dihydroxyacetone-phosphate reductase; NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKKVTMLGAGSWGTALALVLTDNGNEVCVWAHRADLIHQINELHENKDYLPNVKLSTSIKGTTDMKEAVSDADVIIVAVP
TKAIREVLRQAVPFITKKAVFVHVSKGIEPDSLLRISEIMEIELPSDVRKDIVVLSGPSHAEEVGLRHPTTVTASSKSMR
AAEEVQDLFINHNFRVYTNPDIIGVEIGGALKNIIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSGL
TGVGDLIVTCTSVHSRNWRAGNLLGKGYKLEDVLEEMGMVVEGVRTTKAAYQLSKKYDVKMPITEALHQVLFNGQKVETA
VESLMARGKTHEMEDLVNTFENQVK

Sequences:

>Translated_345_residues
MKKVTMLGAGSWGTALALVLTDNGNEVCVWAHRADLIHQINELHENKDYLPNVKLSTSIKGTTDMKEAVSDADVIIVAVP
TKAIREVLRQAVPFITKKAVFVHVSKGIEPDSLLRISEIMEIELPSDVRKDIVVLSGPSHAEEVGLRHPTTVTASSKSMR
AAEEVQDLFINHNFRVYTNPDIIGVEIGGALKNIIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSGL
TGVGDLIVTCTSVHSRNWRAGNLLGKGYKLEDVLEEMGMVVEGVRTTKAAYQLSKKYDVKMPITEALHQVLFNGQKVETA
VESLMARGKTHEMEDLVNTFENQVK
>Mature_345_residues
MKKVTMLGAGSWGTALALVLTDNGNEVCVWAHRADLIHQINELHENKDYLPNVKLSTSIKGTTDMKEAVSDADVIIVAVP
TKAIREVLRQAVPFITKKAVFVHVSKGIEPDSLLRISEIMEIELPSDVRKDIVVLSGPSHAEEVGLRHPTTVTASSKSMR
AAEEVQDLFINHNFRVYTNPDIIGVEIGGALKNIIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSGL
TGVGDLIVTCTSVHSRNWRAGNLLGKGYKLEDVLEEMGMVVEGVRTTKAAYQLSKKYDVKMPITEALHQVLFNGQKVETA
VESLMARGKTHEMEDLVNTFENQVK

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI24307999, Length=346, Percent_Identity=32.3699421965318, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI33695088, Length=347, Percent_Identity=30.835734870317, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1790037, Length=330, Percent_Identity=40.3030303030303, Blast_Score=269, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32564399, Length=346, Percent_Identity=31.2138728323699, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32564403, Length=358, Percent_Identity=31.2849162011173, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI193210136, Length=358, Percent_Identity=31.2849162011173, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17507425, Length=344, Percent_Identity=29.3604651162791, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI193210134, Length=346, Percent_Identity=30.0578034682081, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324513, Length=350, Percent_Identity=26.5714285714286, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320181, Length=341, Percent_Identity=27.2727272727273, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI17136202, Length=356, Percent_Identity=30.6179775280899, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI17136200, Length=344, Percent_Identity=30.8139534883721, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17136204, Length=344, Percent_Identity=30.8139534883721, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI22026922, Length=350, Percent_Identity=26, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45551945, Length=283, Percent_Identity=27.208480565371, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281362270, Length=284, Percent_Identity=27.112676056338, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648969, Length=235, Percent_Identity=28.0851063829787, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_BACSU (P46919)

Other databases:

- EMBL:   U32164
- EMBL:   L47648
- EMBL:   AL009126
- PIR:   H69636
- RefSeq:   NP_390164.1
- ProteinModelPortal:   P46919
- SMR:   P46919
- EnsemblBacteria:   EBBACT00000001021
- GeneID:   938989
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22830
- NMPDR:   fig|224308.1.peg.2287
- GenoList:   BSU22830
- GeneTree:   EBGT00050000002224
- HOGENOM:   HBG586392
- OMA:   NVAKGIE
- PhylomeDB:   P46919
- ProtClustDB:   PRK00094
- BioCyc:   BSUB:BSU22830-MONOMER
- BRENDA:   1.1.1.94
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 37437; Mature: 37437

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 192-192 BINDING 106-106 BINDING 106-106 BINDING 141-141 BINDING 256-256 BINDING 282-282

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVTMLGAGSWGTALALVLTDNGNEVCVWAHRADLIHQINELHENKDYLPNVKLSTSIK
CCEEEEEECCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEECCCC
GTTDMKEAVSDADVIIVAVPTKAIREVLRQAVPFITKKAVFVHVSKGIEPDSLLRISEIM
CCCHHHHHCCCCCEEEEEECHHHHHHHHHHHCCHHHHCEEEEEECCCCCHHHHHHHHHHH
EIELPSDVRKDIVVLSGPSHAEEVGLRHPTTVTASSKSMRAAEEVQDLFINHNFRVYTNP
EECCCCHHCCCEEEEECCCCHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEECC
DIIGVEIGGALKNIIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSGL
CEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCC
TGVGDLIVTCTSVHSRNWRAGNLLGKGYKLEDVLEEMGMVVEGVRTTKAAYQLSKKYDVK
CCHHHHHHEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MPITEALHQVLFNGQKVETAVESLMARGKTHEMEDLVNTFENQVK
CCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVTMLGAGSWGTALALVLTDNGNEVCVWAHRADLIHQINELHENKDYLPNVKLSTSIK
CCEEEEEECCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEECCCC
GTTDMKEAVSDADVIIVAVPTKAIREVLRQAVPFITKKAVFVHVSKGIEPDSLLRISEIM
CCCHHHHHCCCCCEEEEEECHHHHHHHHHHHCCHHHHCEEEEEECCCCCHHHHHHHHHHH
EIELPSDVRKDIVVLSGPSHAEEVGLRHPTTVTASSKSMRAAEEVQDLFINHNFRVYTNP
EECCCCHHCCCEEEEECCCCHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEECC
DIIGVEIGGALKNIIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSGL
CEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCC
TGVGDLIVTCTSVHSRNWRAGNLLGKGYKLEDVLEEMGMVVEGVRTTKAAYQLSKKYDVK
CCHHHHHHEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MPITEALHQVLFNGQKVETAVESLMARGKTHEMEDLVNTFENQVK
CCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592341; 8760912; 9384377