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The map label for this gene is folE

Identifier: 16079335

GI number: 16079335

Start: 2384783

End: 2385355

Strand: Reverse

Name: folE

Synonym: BSU22780

Alternate gene names: 16079335

Gene position: 2385355-2384783 (Counterclockwise)

Preceding gene: 16079336

Following gene: 16079334

Centisome position: 56.58

GC content: 45.72

Gene sequence:

>573_bases
ATGAAAGAAGTTAATAAAGAGCAAATCGAACAAGCTGTTCGTCAAATTTTAGAAGCGATCGGAGAAGACCCGAATAGAGA
AGGGCTTCTTGATACTCCGAAAAGAGTCGCAAAGATGTATGCCGAAGTATTCTCCGGCTTGAATGAAGATCCAAAAGAAC
ATTTCCAGACTATCTTCGGTGAAAACCATGAGGAGCTTGTTCTTGTAAAAGATATAGCGTTTCATTCTATGTGTGAGCAT
CACCTTGTTCCCTTTTATGGAAAAGCACATGTTGCATATATCCCGCGAGGCGGAAAGGTCACAGGACTCAGCAAACTGGC
ACGTGCCGTTGAAGCCGTTGCAAAGCGCCCGCAGCTTCAGGAACGCATCACTTCTACAATTGCAGAAAGCATCGTAGAAA
CGCTTGATCCGCATGGCGTAATGGTAGTGGTTGAAGCGGAACACATGTGCATGACGATGCGCGGTGTAAGAAAACCGGGT
GCGAAAACTGTGACTTCAGCAGTCAGAGGCGTTTTTAAAGATGATGCCGCTGCCCGTGCAGAAGTATTGGAACATATTAA
ACGCCAGGACTAA

Upstream 100 bases:

>100_bases
CAAGAGCATGGGCTTCCTGACAGGGCATTCACTTTGCTTTTAGCGGGGCATATGTGCTAGAATCGAAATTAAATGTATTC
ATTGGTGGAGGACATAGAAC

Downstream 100 bases:

>100_bases
TAAAGATAGAGGGTGCTATAAATGAACCAAAAGCATTCAAGTGATTTTGTCGTCATTAAAGCCGTTGAGGACGGAGTGAA
TGTGATCGGCCTGACAAGAG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKEVNKEQIEQAVRQILEAIGEDPNREGLLDTPKRVAKMYAEVFSGLNEDPKEHFQTIFGENHEELVLVKDIAFHSMCEH
HLVPFYGKAHVAYIPRGGKVTGLSKLARAVEAVAKRPQLQERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPG
AKTVTSAVRGVFKDDAAARAEVLEHIKRQD

Sequences:

>Translated_190_residues
MKEVNKEQIEQAVRQILEAIGEDPNREGLLDTPKRVAKMYAEVFSGLNEDPKEHFQTIFGENHEELVLVKDIAFHSMCEH
HLVPFYGKAHVAYIPRGGKVTGLSKLARAVEAVAKRPQLQERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPG
AKTVTSAVRGVFKDDAAARAEVLEHIKRQD
>Mature_190_residues
MKEVNKEQIEQAVRQILEAIGEDPNREGLLDTPKRVAKMYAEVFSGLNEDPKEHFQTIFGENHEELVLVKDIAFHSMCEH
HLVPFYGKAHVAYIPRGGKVTGLSKLARAVEAVAKRPQLQERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPG
AKTVTSAVRGVFKDDAAARAEVLEHIKRQD

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=193, Percent_Identity=50.259067357513, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI4503949, Length=193, Percent_Identity=50.259067357513, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI66932970, Length=151, Percent_Identity=49.6688741721854, Blast_Score=134, Evalue=3e-32,
Organism=Homo sapiens, GI66932972, Length=152, Percent_Identity=49.3421052631579, Blast_Score=134, Evalue=4e-32,
Organism=Escherichia coli, GI1788476, Length=193, Percent_Identity=37.8238341968912, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560486, Length=189, Percent_Identity=48.6772486772487, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6321706, Length=169, Percent_Identity=49.7041420118343, Blast_Score=170, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24656782, Length=182, Percent_Identity=46.7032967032967, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656796, Length=182, Percent_Identity=46.7032967032967, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24656791, Length=182, Percent_Identity=46.7032967032967, Blast_Score=161, Evalue=2e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 21220; Mature: 21220

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVNKEQIEQAVRQILEAIGEDPNREGLLDTPKRVAKMYAEVFSGLNEDPKEHFQTIFG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
ENHEELVLVKDIAFHSMCEHHLVPFYGKAHVAYIPRGGKVTGLSKLARAVEAVAKRPQLQ
CCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHH
ERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPGAKTVTSAVRGVFKDDAAARA
HHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EVLEHIKRQD
HHHHHHHCCC
>Mature Secondary Structure
MKEVNKEQIEQAVRQILEAIGEDPNREGLLDTPKRVAKMYAEVFSGLNEDPKEHFQTIFG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
ENHEELVLVKDIAFHSMCEHHLVPFYGKAHVAYIPRGGKVTGLSKLARAVEAVAKRPQLQ
CCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHH
ERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPGAKTVTSAVRGVFKDDAAARA
HHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EVLEHIKRQD
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA