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The map label for this gene is trpA

Identifier: 16079320

GI number: 16079320

Start: 2371508

End: 2372311

Strand: Reverse

Name: trpA

Synonym: BSU22630

Alternate gene names: 16079320

Gene position: 2372311-2371508 (Counterclockwise)

Preceding gene: 50812257

Following gene: 255767486

Centisome position: 56.27

GC content: 44.78

Gene sequence:

>804_bases
ATGTTTAAATTGGATCTTCAACCATCAGAAAAATTGTTTATCCCGTTTATTACGGCGGGCGATCCAGTTCCTGAGGTTTC
GATTGAACTGGCGAAGTCACTCCAAAAAGCAGGCGCCACAGCATTGGAGCTTGGTGTTGCATACTCTGACCCGCTTGCAG
ACGGTCCGGTGATCCAGCGGGCTTCAAAGCGGGCGCTTGATCAAGGAATGAATATCGTAAAGGCAATCGAATTAGGCGGA
GAAATGAAAAAAAACGGAGTGAATATTCCGATTATCCTCTTTACGTATTATAATCCTGTGTTACAATTGAACAAAGAATA
CTTTTTCGCTTTACTGCGGGAAAATCATATTGACGGTCTGCTTGTTCCGGATCTGCCATTAGAAGAAAGCAACAGCCTTC
AAGAGGAATGTAAAAGCCATGAGGTGACGTATATTTCTTTAGTTGCGCCGACAAGCGAAAGCCGTTTGAAAACCATTATT
GAACAAGCCGAGGGGTTCGTCTACTGTGTATCTTCTCTGGGTGTGACCGGTGTCCGCAATGAGTTCAATTCATCCGTGTA
CCCGTTCATTCGTACTGTGAAGAATCTCAGCACTGTTCCGGTTGCTGTAGGGTTCGGTATATCAAACCGTGAACAGGTCA
TAAAGATGAATGAAATTAGTGACGGTGTCGTAGTGGGAAGTGCGCTCGTCAGAAAAATAGAAGAATTAAAGGACCGGCTC
ATCAGCGCTGAAACGAGAAATCAGGCGCTGCAGGAGTTTGAGGATTATGCAATGGCGTTTAGCGGCTTGTACAGTTTAAA
ATGA

Upstream 100 bases:

>100_bases
GGAATGGATCGCGGTCAACTCATTCTCGTCTGTTTATCAGGCCGGGGAGACAAGGATGTCAACACATTAATGAATGTATT
GGAAGAAGAGGTGAAAGCCC

Downstream 100 bases:

>100_bases
GGTGACTGATTTGCGTATCAAAGAACATTTAAAACAGCTGAAGCCTTATCAGCCTGGAAAGCCGATTGAAGCAGTGAAAT
CAGAATATGGCTTGGATAAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MFKLDLQPSEKLFIPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQRASKRALDQGMNIVKAIELGG
EMKKNGVNIPIILFTYYNPVLQLNKEYFFALLRENHIDGLLVPDLPLEESNSLQEECKSHEVTYISLVAPTSESRLKTII
EQAEGFVYCVSSLGVTGVRNEFNSSVYPFIRTVKNLSTVPVAVGFGISNREQVIKMNEISDGVVVGSALVRKIEELKDRL
ISAETRNQALQEFEDYAMAFSGLYSLK

Sequences:

>Translated_267_residues
MFKLDLQPSEKLFIPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQRASKRALDQGMNIVKAIELGG
EMKKNGVNIPIILFTYYNPVLQLNKEYFFALLRENHIDGLLVPDLPLEESNSLQEECKSHEVTYISLVAPTSESRLKTII
EQAEGFVYCVSSLGVTGVRNEFNSSVYPFIRTVKNLSTVPVAVGFGISNREQVIKMNEISDGVVVGSALVRKIEELKDRL
ISAETRNQALQEFEDYAMAFSGLYSLK
>Mature_267_residues
MFKLDLQPSEKLFIPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQRASKRALDQGMNIVKAIELGG
EMKKNGVNIPIILFTYYNPVLQLNKEYFFALLRENHIDGLLVPDLPLEESNSLQEECKSHEVTYISLVAPTSESRLKTII
EQAEGFVYCVSSLGVTGVRNEFNSSVYPFIRTVKNLSTVPVAVGFGISNREQVIKMNEISDGVVVGSALVRKIEELKDRL
ISAETRNQALQEFEDYAMAFSGLYSLK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=228, Percent_Identity=35.5263157894737, Blast_Score=134, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6321412, Length=220, Percent_Identity=36.3636363636364, Blast_Score=153, Evalue=3e-38,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29450; Mature: 29450

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLDLQPSEKLFIPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQR
CEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
ASKRALDQGMNIVKAIELGGEMKKNGVNIPIILFTYYNPVLQLNKEYFFALLRENHIDGL
HHHHHHHHCCHHHHHHHHCCCHHHCCCCCEEEEEECCCHHHHHCHHHHHHHHHHCCCCCE
LVPDLPLEESNSLQEECKSHEVTYISLVAPTSESRLKTIIEQAEGFVYCVSSLGVTGVRN
ECCCCCCCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEHHHCCCCCHHH
EFNSSVYPFIRTVKNLSTVPVAVGFGISNREQVIKMNEISDGVVVGSALVRKIEELKDRL
HHCCHHHHHHHHHHCCCCCCEEEECCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHH
ISAETRNQALQEFEDYAMAFSGLYSLK
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKLDLQPSEKLFIPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQR
CEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
ASKRALDQGMNIVKAIELGGEMKKNGVNIPIILFTYYNPVLQLNKEYFFALLRENHIDGL
HHHHHHHHCCHHHHHHHHCCCHHHCCCCCEEEEEECCCHHHHHCHHHHHHHHHHCCCCCE
LVPDLPLEESNSLQEECKSHEVTYISLVAPTSESRLKTIIEQAEGFVYCVSSLGVTGVRN
ECCCCCCCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEHHHCCCCCHHH
EFNSSVYPFIRTVKNLSTVPVAVGFGISNREQVIKMNEISDGVVVGSALVRKIEELKDRL
HHCCHHHHHHHHHHCCCCCCEEEECCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHH
ISAETRNQALQEFEDYAMAFSGLYSLK
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA