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The map label for this gene is aspB

Identifier: 16079294

GI number: 16079294

Start: 2347660

End: 2348841

Strand: Reverse

Name: aspB

Synonym: BSU22370

Alternate gene names: 16079294

Gene position: 2348841-2347660 (Counterclockwise)

Preceding gene: 16079295

Following gene: 16079293

Centisome position: 55.72

GC content: 44.08

Gene sequence:

>1182_bases
TTGAAACTGGCAAAAAGAGTATCCGCATTAACACCATCAACCACACTGGCAATCACAGCGAAAGCGAAAGAACTGAAAGC
CGCAGGCCATGATGTCATCGGCTTAGGAGCAGGCGAGCCTGACTTCAATACACCGCAGCATATTATTGATGCCGCTGTGC
GTTCGATGAACGAGGGCCATACGAAATACACGCCTTCCGGTGGTTTGGCTGAACTGAAAAACAGCATTGCAGAGAAATTT
AAACGCGACCAGAATATTGAATACAAACCGTCTCAAATTATTGTCTGCACTGGTGCCAAACATGCTCTGTATACCCTTTT
CCAAGTGATTTTGGATGAAGAAGATGAAGTTATTATTCCAACGCCTTACTGGGTAAGCTACCCGGAGCAGGTGAAACTCG
CTGGCGGAAAGCCTGTTTATGTAGAAGGGCTTGAGGAAAATCACTTCAAAATTTCGCCTGAGCAGCTGAAAAATGCAATT
ACGGAAAAAACAAAAGCAATCGTTATTAACTCTCCAAGCAACCCGACTGGTGTCATGTATACGGAAGAAGAACTATCTGC
GCTCGGTGAAGTGTGCCTTGAACATGATATCTTGATTGTGTCAGATGAAATTTATGAAAAACTTACATACGGCGGAAAGA
AACATGTTTCCATTGCACAGCTGTCAGACAGACTGAAAGAGCAAACGGTCATTATAAACGGCGTATCGAAGTCCCACAGC
ATGACAGGCTGGAGAATCGGTTATGCGGCAGGCTCTGAAGACATTATTAAAGCGATGACGAACCTTGCAAGCCACAGCAC
GTCAAACCCGACATCAATCGCACAATACGGAGCGATTGCTGCTTATAACGGGCCTTCTGAGCCATTGGAAGAAATGAGAG
AAGCATTTGAACATAGATTAAATACGATCTATGCAAAGCTCATAGAAATCCCAGGGTTCAGCTGCGTGAAGCCGGAAGGT
GCTTTTTACTTGTTCCCAAATGCAAAAGAGGCAGCTCAATCTTGCGGTTTCAAAGATGTAGATGAATTTGTTAAAGCGCT
TCTCGAGGAGGAAAAAGTTGCGATTGTTCCAGGATCCGGATTTGGCTCTCCTGAGAATGTCCGTCTTTCTTATGCAACAT
CGTTAGATCTTCTTGAAGAAGCCATTGAAAGAATCAAGCGTTTTGTAGAAAAACATAGCTAA

Upstream 100 bases:

>100_bases
TTAGACAAAGAAGGGCAATACAGTTTAAGTTATGTGGACTTTACAACTGGAAAGATTCTCAAAAATATCACGCCTTAAGA
CGAATTAGGGGGAGTTCAAG

Downstream 100 bases:

>100_bases
CAGATCAAAAAGCGGCTGACAGAAAAGCCGCTTTTTATCTGCATTGACCCGGTTAGTTAAGAGCGGGTATAATAAAGTGA
TGATAAAAGAGTTCGCGGGG

Product: aspartate aminotransferase

Products: NA

Alternate protein names: AspAT; Transaminase A

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKLAKRVSALTPSTTLAITAKAKELKAAGHDVIGLGAGEPDFNTPQHIIDAAVRSMNEGHTKYTPSGGLAELKNSIAEKF
KRDQNIEYKPSQIIVCTGAKHALYTLFQVILDEEDEVIIPTPYWVSYPEQVKLAGGKPVYVEGLEENHFKISPEQLKNAI
TEKTKAIVINSPSNPTGVMYTEEELSALGEVCLEHDILIVSDEIYEKLTYGGKKHVSIAQLSDRLKEQTVIINGVSKSHS
MTGWRIGYAAGSEDIIKAMTNLASHSTSNPTSIAQYGAIAAYNGPSEPLEEMREAFEHRLNTIYAKLIEIPGFSCVKPEG
AFYLFPNAKEAAQSCGFKDVDEFVKALLEEEKVAIVPGSGFGSPENVRLSYATSLDLLEEAIERIKRFVEKHS

Sequences:

>Translated_393_residues
MKLAKRVSALTPSTTLAITAKAKELKAAGHDVIGLGAGEPDFNTPQHIIDAAVRSMNEGHTKYTPSGGLAELKNSIAEKF
KRDQNIEYKPSQIIVCTGAKHALYTLFQVILDEEDEVIIPTPYWVSYPEQVKLAGGKPVYVEGLEENHFKISPEQLKNAI
TEKTKAIVINSPSNPTGVMYTEEELSALGEVCLEHDILIVSDEIYEKLTYGGKKHVSIAQLSDRLKEQTVIINGVSKSHS
MTGWRIGYAAGSEDIIKAMTNLASHSTSNPTSIAQYGAIAAYNGPSEPLEEMREAFEHRLNTIYAKLIEIPGFSCVKPEG
AFYLFPNAKEAAQSCGFKDVDEFVKALLEEEKVAIVPGSGFGSPENVRLSYATSLDLLEEAIERIKRFVEKHS
>Mature_393_residues
MKLAKRVSALTPSTTLAITAKAKELKAAGHDVIGLGAGEPDFNTPQHIIDAAVRSMNEGHTKYTPSGGLAELKNSIAEKF
KRDQNIEYKPSQIIVCTGAKHALYTLFQVILDEEDEVIIPTPYWVSYPEQVKLAGGKPVYVEGLEENHFKISPEQLKNAI
TEKTKAIVINSPSNPTGVMYTEEELSALGEVCLEHDILIVSDEIYEKLTYGGKKHVSIAQLSDRLKEQTVIINGVSKSHS
MTGWRIGYAAGSEDIIKAMTNLASHSTSNPTSIAQYGAIAAYNGPSEPLEEMREAFEHRLNTIYAKLIEIPGFSCVKPEG
AFYLFPNAKEAAQSCGFKDVDEFVKALLEEEKVAIVPGSGFGSPENVRLSYATSLDLLEEAIERIKRFVEKHS

Specific function: Unknown

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI95147551, Length=399, Percent_Identity=27.3182957393484, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI169881279, Length=399, Percent_Identity=27.3182957393484, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI56713256, Length=418, Percent_Identity=27.511961722488, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI56713254, Length=416, Percent_Identity=27.6442307692308, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI169881281, Length=396, Percent_Identity=23.7373737373737, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI4507369, Length=377, Percent_Identity=25.4641909814324, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4885351, Length=352, Percent_Identity=24.7159090909091, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI215599424, Length=366, Percent_Identity=24.3169398907104, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI19263340, Length=366, Percent_Identity=24.3169398907104, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI187936925, Length=195, Percent_Identity=26.1538461538462, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI14211921, Length=195, Percent_Identity=26.1538461538462, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788627, Length=389, Percent_Identity=29.305912596401, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1786816, Length=376, Percent_Identity=27.6595744680851, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1788722, Length=386, Percent_Identity=29.7927461139896, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787909, Length=375, Percent_Identity=22.6666666666667, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI48994949, Length=330, Percent_Identity=24.8484848484848, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994472, Length=429, Percent_Identity=28.9044289044289, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI71994476, Length=429, Percent_Identity=28.9044289044289, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17567369, Length=398, Percent_Identity=27.6381909547739, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17567663, Length=375, Percent_Identity=26.1333333333333, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71981209, Length=366, Percent_Identity=21.5846994535519, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322401, Length=392, Percent_Identity=28.5714285714286, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6323118, Length=370, Percent_Identity=27.5675675675676, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320317, Length=341, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28573069, Length=419, Percent_Identity=26.9689737470167, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24646114, Length=419, Percent_Identity=26.9689737470167, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI28573067, Length=419, Percent_Identity=26.9689737470167, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI28573065, Length=419, Percent_Identity=26.9689737470167, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI18859735, Length=385, Percent_Identity=25.4545454545455, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24641770, Length=393, Percent_Identity=24.1730279898219, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641760, Length=393, Percent_Identity=24.1730279898219, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641768, Length=393, Percent_Identity=24.1730279898219, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641766, Length=393, Percent_Identity=24.1730279898219, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641764, Length=393, Percent_Identity=24.1730279898219, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI45551451, Length=393, Percent_Identity=24.1730279898219, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AAT1_BACSU (P53001)

Other databases:

- EMBL:   L47709
- EMBL:   AL009126
- PIR:   C69591
- RefSeq:   NP_390118.1
- ProteinModelPortal:   P53001
- SMR:   P53001
- EnsemblBacteria:   EBBACT00000002367
- GeneID:   939037
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22370
- NMPDR:   fig|224308.1.peg.2241
- GenoList:   BSU22370
- GeneTree:   EBGT00070000031834
- HOGENOM:   HBG645860
- OMA:   AYKRTIV
- PhylomeDB:   P53001
- ProtClustDB:   PRK05764
- BioCyc:   BSUB:BSU22370-MONOMER
- BRENDA:   2.6.1.1
- GO:   GO:0005737
- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PRINTS:   PR00753

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.1

Molecular weight: Translated: 43088; Mature: 43088

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 38-38 BINDING 124-124 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAKRVSALTPSTTLAITAKAKELKAAGHDVIGLGAGEPDFNTPQHIIDAAVRSMNEGH
CCHHHHHHHCCCCCEEEEEECHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCC
TKYTPSGGLAELKNSIAEKFKRDQNIEYKPSQIIVCTGAKHALYTLFQVILDEEDEVIIP
CEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEC
TPYWVSYPEQVKLAGGKPVYVEGLEENHFKISPEQLKNAITEKTKAIVINSPSNPTGVMY
CCCCCCCCCCEEECCCCEEEEECCCCCCEEECHHHHHHHHHHCCCEEEEECCCCCCCEEE
TEEELSALGEVCLEHDILIVSDEIYEKLTYGGKKHVSIAQLSDRLKEQTVIINGVSKSHS
EHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEEECCCCCCC
MTGWRIGYAAGSEDIIKAMTNLASHSTSNPTSIAQYGAIAAYNGPSEPLEEMREAFEHRL
CCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHCCEEECCCCHHHHHHHHHHHHHHH
NTIYAKLIEIPGFSCVKPEGAFYLFPNAKEAAQSCGFKDVDEFVKALLEEEKVAIVPGSG
HHHHHHHHHCCCCCEECCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCC
FGSPENVRLSYATSLDLLEEAIERIKRFVEKHS
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLAKRVSALTPSTTLAITAKAKELKAAGHDVIGLGAGEPDFNTPQHIIDAAVRSMNEGH
CCHHHHHHHCCCCCEEEEEECHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCC
TKYTPSGGLAELKNSIAEKFKRDQNIEYKPSQIIVCTGAKHALYTLFQVILDEEDEVIIP
CEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEC
TPYWVSYPEQVKLAGGKPVYVEGLEENHFKISPEQLKNAITEKTKAIVINSPSNPTGVMY
CCCCCCCCCCEEECCCCEEEEECCCCCCEEECHHHHHHHHHHCCCEEEEECCCCCCCEEE
TEEELSALGEVCLEHDILIVSDEIYEKLTYGGKKHVSIAQLSDRLKEQTVIINGVSKSHS
EHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEEECCCCCCC
MTGWRIGYAAGSEDIIKAMTNLASHSTSNPTSIAQYGAIAAYNGPSEPLEEMREAFEHRL
CCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHCCEEECCCCHHHHHHHHHHHHHHH
NTIYAKLIEIPGFSCVKPEGAFYLFPNAKEAAQSCGFKDVDEFVKALLEEEKVAIVPGSG
HHHHHHHHHCCCCCEECCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCC
FGSPENVRLSYATSLDLLEEAIERIKRFVEKHS
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377