Click here to switch to the map view.

The map label for this gene is exoA

Identifier: 16079259

GI number: 16079259

Start: 2310995

End: 2311885

Strand: Reverse

Name: exoA

Synonym: BSU22010

Alternate gene names: 16079259

Gene position: 2311885-2310995 (Counterclockwise)

Preceding gene: 255767481

Following gene: 161511070

Centisome position: 54.84

GC content: 45.23

Gene sequence:

>891_bases
ATGAATAATAATAAACTATTGCTGGTTGACGGCATGGCTCTTTTATTCCGGGCCTTTTTTGCCACGGCCGTACATCGCAA
CTTTATGATAAATGACAGCGGGGTGCCGACGAACGGCGTCAACGGCTTTTTAAAGCATTTGATTACAGCTGTGGAAACAT
TCCAGCCGACACACGTCGTCTGCTGCTGGGACATGGGGAGCAAAACGTACCGAAATGACTTGTTCCAAGATTATAAGGCC
AACCGCAGCGCGCCGCCTGTGGAGCTGATTCCGCAGTTTGATTTGGCAAAGGAAGCAGCCGCAGAGCTTGGAATCATGAA
TATTGGCTTTGCCGGCTATGAAGCGGATGACTGTATCGGTACTCTTGCCGATTTATTTGCGAATGAAGCGGACATTACGG
TGGTAACAGGGGACAGAGATTTACTTCAGCTGTTAACGGATAAAGTAAGCGTTGCTTTGCTTCAAAAAGGAATCGGAAAT
TATAAAGTCTATACAAAAGAGACTTTTTATGAAGAAACAGGCGTAATGCCGAAGGCTTTGATTGATATTAAAGCGCTTAT
GGGTGATTCCAGTGATAACTATCCCGGAGTCAAAGGCATTGGAGAAAAAACAGCCTACAAACTGATTCGTGAATACGAAA
CGATTGACCGTTTATTGGAAAATCTTTCTCTTCTTCCAAAAGGACAGCAGGGAAAAATTCAGCAAGGTTTAAGCGATTTG
GAGATGTCGAGGAAGCTTGCGGAAATCCATTGCTCAGTGCCGCTGGCCTGCACACTGAAAGACGCTTTATTTACTTTGCA
AATGGAGCAGGCGGCTGACATGCTTCGCCGCCACCAAATTAAAGGAATTGAGCGTATGCTTGAAAAGCTGAACGCTAGAG
AGATCGTTTAG

Upstream 100 bases:

>100_bases
TAAATATTCAAACAGTATCTCTAATTAAAGAGATACTGTTTTTTATTTTTGGGCTGCTTTATGATAAAATAGAAGGAATG
AAAGAAAAGGGAGTTATTTG

Downstream 100 bases:

>100_bases
ATCCTCTCTAGCGTTTTGTATTGCTTTTTTTTGCTCTCTCCTCAAGCTCTGTTTTAGGGTCATTATGTGTATTGCCTTCC
AGGTCGCCTTGCGGAGTAAC

Product: 5'3'-exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNNNKLLLVDGMALLFRAFFATAVHRNFMINDSGVPTNGVNGFLKHLITAVETFQPTHVVCCWDMGSKTYRNDLFQDYKA
NRSAPPVELIPQFDLAKEAAAELGIMNIGFAGYEADDCIGTLADLFANEADITVVTGDRDLLQLLTDKVSVALLQKGIGN
YKVYTKETFYEETGVMPKALIDIKALMGDSSDNYPGVKGIGEKTAYKLIREYETIDRLLENLSLLPKGQQGKIQQGLSDL
EMSRKLAEIHCSVPLACTLKDALFTLQMEQAADMLRRHQIKGIERMLEKLNAREIV

Sequences:

>Translated_296_residues
MNNNKLLLVDGMALLFRAFFATAVHRNFMINDSGVPTNGVNGFLKHLITAVETFQPTHVVCCWDMGSKTYRNDLFQDYKA
NRSAPPVELIPQFDLAKEAAAELGIMNIGFAGYEADDCIGTLADLFANEADITVVTGDRDLLQLLTDKVSVALLQKGIGN
YKVYTKETFYEETGVMPKALIDIKALMGDSSDNYPGVKGIGEKTAYKLIREYETIDRLLENLSLLPKGQQGKIQQGLSDL
EMSRKLAEIHCSVPLACTLKDALFTLQMEQAADMLRRHQIKGIERMLEKLNAREIV
>Mature_296_residues
MNNNKLLLVDGMALLFRAFFATAVHRNFMINDSGVPTNGVNGFLKHLITAVETFQPTHVVCCWDMGSKTYRNDLFQDYKA
NRSAPPVELIPQFDLAKEAAAELGIMNIGFAGYEADDCIGTLADLFANEADITVVTGDRDLLQLLTDKVSVALLQKGIGN
YKVYTKETFYEETGVMPKALIDIKALMGDSSDNYPGVKGIGEKTAYKLIREYETIDRLLENLSLLPKGQQGKIQQGLSDL
EMSRKLAEIHCSVPLACTLKDALFTLQMEQAADMLRRHQIKGIERMLEKLNAREIV

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain

Homologues:

Organism=Escherichia coli, GI1790294, Length=284, Percent_Identity=33.8028169014084, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI226510970, Length=186, Percent_Identity=31.7204301075269, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): EX53_BACSU (P54161)

Other databases:

- EMBL:   L77246
- EMBL:   AL009126
- PIR:   H69933
- RefSeq:   NP_390083.1
- ProteinModelPortal:   P54161
- SMR:   P54161
- EnsemblBacteria:   EBBACT00000002859
- GeneID:   939070
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22010
- NMPDR:   fig|224308.1.peg.2206
- GenoList:   BSU22010
- GeneTree:   EBGT00050000001151
- HOGENOM:   HBG701384
- OMA:   IPSYKAH
- ProtClustDB:   CLSK2460929
- BioCyc:   BSUB:BSU22010-MONOMER
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- SMART:   SM00475
- SMART:   SM00279

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; SSF47807 5_3_exo_C

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 32945; Mature: 32945

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNKLLLVDGMALLFRAFFATAVHRNFMINDSGVPTNGVNGFLKHLITAVETFQPTHVV
CCCCCEEEECCHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
CCWDMGSKTYRNDLFQDYKANRSAPPVELIPQFDLAKEAAAELGIMNIGFAGYEADDCIG
EEEECCCHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
TLADLFANEADITVVTGDRDLLQLLTDKVSVALLQKGIGNYKVYTKETFYEETGVMPKAL
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHHH
IDIKALMGDSSDNYPGVKGIGEKTAYKLIREYETIDRLLENLSLLPKGQQGKIQQGLSDL
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
EMSRKLAEIHCSVPLACTLKDALFTLQMEQAADMLRRHQIKGIERMLEKLNAREIV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNNKLLLVDGMALLFRAFFATAVHRNFMINDSGVPTNGVNGFLKHLITAVETFQPTHVV
CCCCCEEEECCHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
CCWDMGSKTYRNDLFQDYKANRSAPPVELIPQFDLAKEAAAELGIMNIGFAGYEADDCIG
EEEECCCHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
TLADLFANEADITVVTGDRDLLQLLTDKVSVALLQKGIGNYKVYTKETFYEETGVMPKAL
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHHH
IDIKALMGDSSDNYPGVKGIGEKTAYKLIREYETIDRLLENLSLLPKGQQGKIQQGLSDL
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
EMSRKLAEIHCSVPLACTLKDALFTLQMEQAADMLRRHQIKGIERMLEKLNAREIV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377