Click here to switch to the map view.

The map label for this gene is yocH

Identifier: 16078981

GI number: 16078981

Start: 2092899

End: 2093762

Strand: Reverse

Name: yocH

Synonym: BSU19210

Alternate gene names: 16078981

Gene position: 2093762-2092899 (Counterclockwise)

Preceding gene: 16078982

Following gene: 16078975

Centisome position: 49.67

GC content: 41.67

Gene sequence:

>864_bases
ATGAAGAAGACGATTATGTCCTTTGTGGCAGTTGCTGCACTTTCAACAACTGCATTCGGAGCTCACGCTTCTGCAAAAGA
AATTACGGTGCAAAAGGGTGATACGCTCTGGGGAATCTCTCAGAAAAATGGAGTGAACCTAAAGGACTTAAAAGAATGGA
ACAAGTTAACTTCTGATAAAATCATTGCAGGAGAAAAACTGACCATTTCTTCAGAAGAAACAACCACGACTGGGCAATAT
ACAATTAAAGCGGGAGATACGCTTTCTAAGATTGCTCAAAAATTCGGCACGACAGTCAACAACCTGAAAGTTTGGAATAA
CTTAAGCTCTGACATGATCTATGCTGGATCAACTCTTTCAGTAAAAGGTCAGGCAACAGCAGCAAACACAGCTACTGAGA
ATGCGCAAACAAACGCACCTCAAGCTGCGCCAAAACAAGAGGCTGTGCAAAAAGAGCAACCTAAACAAGAAGCTGTACAA
CAGCAACCTAAACAAGAAACAAAGGCTGAGGCAGAAACTTCTGTTAACACAGAGGAGAAGGCTGTTCAATCAAATACAAA
TAATCAAGAAGCTTCAAAAGAACTAACAGTTACTGCTACTGCTTACACTGCTAACGACGGCGGCATTTCCGGCGTTACAG
CTACAGGCATTGACTTAAATAAGAACCCGAACGCGAAAGTTATTGCGGTTGATCCTAATGTAATTCCGCTAGGTTCTAAA
GTCTATGTTGAAGGCTACGGGGAAGCAACAGCAGCAGACACTGGCGGCGCTATTAAAGGAAATAAAATCGACGTATTTGT
ACCAAGTAAAAGTGATGCTTCTAATTGGGGCGTCAAAACTGTAAGTGTTAAAGTCTTAAACTAA

Upstream 100 bases:

>100_bases
TAATCTAAATCATATCTTTTTGTTATTGATTTGACATTTACGTGTGTTACGATATCACCTGTTAGCCGCCAATAAAAAGC
TAACAAGGGAGGATTTACTT

Downstream 100 bases:

>100_bases
TAATATAGATTGTGATAAAAGACATAAGCTTTTGGCTTGTGTCTTTTTTTGTTCTGCTTAGAGCTGTTTATATCATATGC
TGATAAAAGAAAGTGGTCCC

Product: exported cell wall-binding protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKKTIMSFVAVAALSTTAFGAHASAKEITVQKGDTLWGISQKNGVNLKDLKEWNKLTSDKIIAGEKLTISSEETTTTGQY
TIKAGDTLSKIAQKFGTTVNNLKVWNNLSSDMIYAGSTLSVKGQATAANTATENAQTNAPQAAPKQEAVQKEQPKQEAVQ
QQPKQETKAEAETSVNTEEKAVQSNTNNQEASKELTVTATAYTANDGGISGVTATGIDLNKNPNAKVIAVDPNVIPLGSK
VYVEGYGEATAADTGGAIKGNKIDVFVPSKSDASNWGVKTVSVKVLN

Sequences:

>Translated_287_residues
MKKTIMSFVAVAALSTTAFGAHASAKEITVQKGDTLWGISQKNGVNLKDLKEWNKLTSDKIIAGEKLTISSEETTTTGQY
TIKAGDTLSKIAQKFGTTVNNLKVWNNLSSDMIYAGSTLSVKGQATAANTATENAQTNAPQAAPKQEAVQKEQPKQEAVQ
QQPKQETKAEAETSVNTEEKAVQSNTNNQEASKELTVTATAYTANDGGISGVTATGIDLNKNPNAKVIAVDPNVIPLGSK
VYVEGYGEATAADTGGAIKGNKIDVFVPSKSDASNWGVKTVSVKVLN
>Mature_287_residues
MKKTIMSFVAVAALSTTAFGAHASAKEITVQKGDTLWGISQKNGVNLKDLKEWNKLTSDKIIAGEKLTISSEETTTTGQY
TIKAGDTLSKIAQKFGTTVNNLKVWNNLSSDMIYAGSTLSVKGQATAANTATENAQTNAPQAAPKQEAVQKEQPKQEAVQ
QQPKQETKAEAETSVNTEEKAVQSNTNNQEASKELTVTATAYTANDGGISGVTATGIDLNKNPNAKVIAVDPNVIPLGSK
VYVEGYGEATAADTGGAIKGNKIDVFVPSKSDASNWGVKTVSVKVLN

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG1388

COG function: function code M; FOG: LysM repeat

Gene ontology:

Cell location: Secreted, cell wall. Note=Released into the medium

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats

Homologues:

Organism=Escherichia coli, GI1786405, Length=90, Percent_Identity=34.4444444444444, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YOCH_BACSU (O34669)

Other databases:

- EMBL:   AF027868
- EMBL:   AL009126
- PIR:   E69901
- RefSeq:   NP_389802.1
- ProteinModelPortal:   O34669
- EnsemblBacteria:   EBBACT00000002457
- GeneID:   939673
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19210
- NMPDR:   fig|224308.1.peg.1925
- GenoList:   BSU19210
- GeneTree:   EBGT00070000032031
- HOGENOM:   HBG399366
- OMA:   KEITVQK
- ProtClustDB:   CLSK887415
- BioCyc:   BSUB:BSU19210-MONOMER
- InterPro:   IPR010611
- InterPro:   IPR014733
- InterPro:   IPR018392
- InterPro:   IPR002482
- Gene3D:   G3DSA:2.40.40.10
- SMART:   SM00257

Pfam domain/function: PF06725 3D; PF01476 LysM

EC number: 3.2.1.- [C]

Molecular weight: Translated: 30182; Mature: 30182

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIMSFVAVAALSTTAFGAHASAKEITVQKGDTLWGISQKNGVNLKDLKEWNKLTSDK
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCC
IIAGEKLTISSEETTTTGQYTIKAGDTLSKIAQKFGTTVNNLKVWNNLSSDMIYAGSTLS
EECCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCHHCEEEECCCCCCEEEECCEEE
VKGQATAANTATENAQTNAPQAAPKQEAVQKEQPKQEAVQQQPKQETKAEAETSVNTEEK
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCHHH
AVQSNTNNQEASKELTVTATAYTANDGGISGVTATGIDLNKNPNAKVIAVDPNVIPLGSK
HHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECEECCCCCCEEEEEECCCEEECCCE
VYVEGYGEATAADTGGAIKGNKIDVFVPSKSDASNWGVKTVSVKVLN
EEEEECCCCCCCCCCCCEECCEEEEEECCCCCCCCCCEEEEEEEEEC
>Mature Secondary Structure
MKKTIMSFVAVAALSTTAFGAHASAKEITVQKGDTLWGISQKNGVNLKDLKEWNKLTSDK
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCC
IIAGEKLTISSEETTTTGQYTIKAGDTLSKIAQKFGTTVNNLKVWNNLSSDMIYAGSTLS
EECCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCHHCEEEECCCCCCEEEECCEEE
VKGQATAANTATENAQTNAPQAAPKQEAVQKEQPKQEAVQQQPKQETKAEAETSVNTEEK
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCHHH
AVQSNTNNQEASKELTVTATAYTANDGGISGVTATGIDLNKNPNAKVIAVDPNVIPLGSK
HHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECEECCCCCCEEEEEECCCEEECCCE
VYVEGYGEATAADTGGAIKGNKIDVFVPSKSDASNWGVKTVSVKVLN
EEEEECCCCCCCCCCCCEECCEEEEEECCCCCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377