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The map label for this gene is csaA

Identifier: 16078964

GI number: 16078964

Start: 2079214

End: 2079546

Strand: Reverse

Name: csaA

Synonym: BSU19040

Alternate gene names: 16078964

Gene position: 2079546-2079214 (Counterclockwise)

Preceding gene: 16078965

Following gene: 255767449

Centisome position: 49.33

GC content: 48.95

Gene sequence:

>333_bases
ATGGCAGTTATTGATGACTTTGAGAAATTGGATATCAGAACGGGAACAATTGTAAAAGCGGAAGAATTTCCTGAAGCAAG
AGTACCGGCAATCAAGCTTGTGATAGATTTTGGGACTGAAATCGGCATAAAACAATCGAGCGCACAAATCACGAAGCGTT
ACAAGCCGGAAGGTCTCATCAACAAGCAAGTCATAGCAGTCGTGAACTTTCCGCCCCGCCGGATCGCCGGATTTAAATCG
GAAGTCTTGGTCCTCGGCGGCATACCCGGCCAGGGCGACGTCGTCCTTTTGCAGCCGGATCAGCCTGTCCCAAACGGCAC
AAAAATCGGATAA

Upstream 100 bases:

>100_bases
CTGTATCGAAACACCTTATATTCGGATAAAAAATGATGGCTCTTTAGTTAAAAGAATATTGTATATCTCCTTTACAATAG
GAGAAAAAGGAGTTATTGAT

Downstream 100 bases:

>100_bases
CGCAAAAAAAGACGTTTGCCTAAGGCAAACGTCTTTGCGTATTGAGGCGATGCACCACACGCCACATTCGTGTGTGCCGT
AAAGGCATGTACATCGGCTT

Product: molecular chaperone

Products: AMP; diphosphate +L-methionyl-tRNAMet [C]

Alternate protein names: NA

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MAVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLINKQVIAVVNFPPRRIAGFKS
EVLVLGGIPGQGDVVLLQPDQPVPNGTKIG

Sequences:

>Translated_110_residues
MAVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLINKQVIAVVNFPPRRIAGFKS
EVLVLGGIPGQGDVVLLQPDQPVPNGTKIG
>Mature_109_residues
AVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLINKQVIAVVNFPPRRIAGFKSE
VLVLGGIPGQGDVVLLQPDQPVPNGTKIG

Specific function: Could serves as a molecular chaperone for exported proteins or alternatively acts by stabilizing the secA protein

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

None

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): CSAA_BACSU (P37584)

Other databases:

- EMBL:   X58387
- EMBL:   AF027868
- EMBL:   AL009126
- PIR:   E69607
- RefSeq:   NP_389785.1
- PDB:   2NZH
- PDB:   2NZO
- PDBsum:   2NZH
- PDBsum:   2NZO
- ProteinModelPortal:   P37584
- SMR:   P37584
- EnsemblBacteria:   EBBACT00000000331
- GeneID:   939631
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19040
- NMPDR:   fig|224308.1.peg.1908
- GenoList:   BSU19040
- GeneTree:   EBGT00050000000803
- HOGENOM:   HBG477532
- OMA:   AQITVHY
- ProtClustDB:   CLSK2752418
- BioCyc:   BSUB:BSU19040-MONOMER
- GO:   GO:0005737
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR008231
- InterPro:   IPR002547
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR02222

Pfam domain/function: PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB

EC number: 6.1.1.10 [C]

Molecular weight: Translated: 11907; Mature: 11776

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLI
CCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCCCCC
NKQVIAVVNFPPRRIAGFKSEVLVLGGIPGQGDVVLLQPDQPVPNGTKIG
CCEEEEEEECCCHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLI
CCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCCCCC
NKQVIAVVNFPPRRIAGFKSEVLVLGGIPGQGDVVLLQPDQPVPNGTKIG
CCEEEEEEECCCHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mn2+; Zn2+ [C]

Kcat value (1/min): 198 [C]

Specific activity: 66.7

Km value (mM): 0.48 {Met}} 0.078 {Met}} 0.022 {Met}} 0.02 {Met}} 0.55 {ATP}} 0.41 {ATP}} 0.0009 {tRNA}} [C]

Substrates: ATP; @METH01.txt*L-methionine!; tRNAMet [C]

Specific reaction: ATP + @METH01.txt*L-methionine! + tRNAMet = AMP + diphosphate +L-methionyl-tRNAMet [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Dipyridyl; Adenosine; NH4+; Periodate-oxidized ATP; Periodate-oxidized tRNA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435734; 9384377