The gene/protein map for NC_010170 is currently unavailable.
Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is yngF

Identifier: 16078883

GI number: 16078883

Start: 1951228

End: 1952010

Strand: Reverse

Name: yngF

Synonym: BSU18220

Alternate gene names: 16078883

Gene position: 1952010-1951228 (Counterclockwise)

Preceding gene: 16078884

Following gene: 255767426

Centisome position: 46.3

GC content: 47.89

Gene sequence:

>783_bases
ATGGGAGATTCTATTCTTTTTACTGTTAAAAATGAACATATGGCGTTGATCACCTTAAACAGGCCTCAGGCAGCAAATGC
TCTTTCAGCGGAAATGCTTAGAAACCTGCAAATGATTATCCAGGAAATTGAATTTAACTCAAACATCCGTTGCGTCATCC
TCACAGGCACCGGTGAAAAAGCGTTTTGTGCAGGGGCAGACCTGAAGGAACGGATAAAACTGAAAGAAGATCAGGTTCTG
GAAAGTGTATCTCTCATTCAAAGAACGGCGGCTTTACTTGATGCCTTGCCGCAGCCGGTCATAGCTGCGATAAATGGAAG
CGCATTAGGCGGCGGACTAGAATTGGCATTGGCATGCGACCTTCGAATCGCAACTGAAGCAGCTGTGCTGGGACTTCCGG
AAACAGGGTTAGCTATTATCCCGGGCGCTGGAGGGACCCAAAGGCTGCCCCGGCTGATTGGCAGAGGAAAAGCAAAAGAA
TTCATTTATACAGGCAGACGCGTGACCGCACACGAAGCAAAAGAAATCGGCCTTGTAGAGCATGTCACGGCTCCTTGTGA
CCTTATGCCAAAAGCAGAGGAACTGGCCGCAGCCATTTCTGCCAACGGACCGATCGCTGTCCGTCAGGCTAAATTTGCAA
TCAATAAAGGATTGGAGACAGATCTTGCTACAGGCCTTGCGATTGAACAAAAAGCGTATGAACAAACCATCCCGACAAAA
GACAGGAGAGAAGGGCTTCAGGCCTTTCAAGAAAAAAGACGGGCCGTATACAAGGGAATATAA

Upstream 100 bases:

>100_bases
TATCTGCGGCCAAATGGATTGAAGAAAAAATGGGCAAACCGCTGCCGAGCAGAAATTTACAGGTGTTTAAATCATCTTGA
TAAAAGGGAGGACGCATACA

Downstream 100 bases:

>100_bases
AAGGGAGGCAATGCTGATGGATTATGAAAAGGAACGAACAGAACGGGCTGAACGGATTCGAAAAGGCGGAGCGGAAAAGT
ATCATCAAAGCAATCGGGAA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGDSILFTVKNEHMALITLNRPQAANALSAEMLRNLQMIIQEIEFNSNIRCVILTGTGEKAFCAGADLKERIKLKEDQVL
ESVSLIQRTAALLDALPQPVIAAINGSALGGGLELALACDLRIATEAAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKE
FIYTGRRVTAHEAKEIGLVEHVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDLATGLAIEQKAYEQTIPTK
DRREGLQAFQEKRRAVYKGI

Sequences:

>Translated_260_residues
MGDSILFTVKNEHMALITLNRPQAANALSAEMLRNLQMIIQEIEFNSNIRCVILTGTGEKAFCAGADLKERIKLKEDQVL
ESVSLIQRTAALLDALPQPVIAAINGSALGGGLELALACDLRIATEAAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKE
FIYTGRRVTAHEAKEIGLVEHVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDLATGLAIEQKAYEQTIPTK
DRREGLQAFQEKRRAVYKGI
>Mature_259_residues
GDSILFTVKNEHMALITLNRPQAANALSAEMLRNLQMIIQEIEFNSNIRCVILTGTGEKAFCAGADLKERIKLKEDQVLE
SVSLIQRTAALLDALPQPVIAAINGSALGGGLELALACDLRIATEAAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKEF
IYTGRRVTAHEAKEIGLVEHVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDLATGLAIEQKAYEQTIPTKD
RREGLQAFQEKRRAVYKGI

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=48.4251968503937, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=41.3654618473896, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI150378541, Length=247, Percent_Identity=37.6518218623482, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI20127408, Length=262, Percent_Identity=35.4961832061069, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=39.2265193370166, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI70995211, Length=264, Percent_Identity=29.5454545454545, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=28.099173553719, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=32.4468085106383, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=32.4468085106383, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=28.6324786324786, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=28.6324786324786, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136756, Length=218, Percent_Identity=27.5229357798165, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI221136753, Length=218, Percent_Identity=27.5229357798165, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI221307494, Length=218, Percent_Identity=27.5229357798165, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI261878539, Length=75, Percent_Identity=53.3333333333333, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI116235487, Length=181, Percent_Identity=28.1767955801105, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI25453481, Length=199, Percent_Identity=27.1356783919598, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=199, Percent_Identity=27.1356783919598, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4757968, Length=185, Percent_Identity=27.5675675675676, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI49355787, Length=185, Percent_Identity=27.5675675675676, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4757966, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI51538581, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=25.1141552511416, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=25.1141552511416, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260274832, Length=219, Percent_Identity=25.1141552511416, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI157694520, Length=204, Percent_Identity=28.921568627451, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=39.1472868217054, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=35.0943396226415, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788682, Length=214, Percent_Identity=40.1869158878505, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=30.6201550387597, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1790281, Length=221, Percent_Identity=35.7466063348416, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=33.8403041825095, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=28.2352941176471, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=229, Percent_Identity=43.2314410480349, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=44.6280991735537, Blast_Score=193, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=41.7004048582996, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17534483, Length=224, Percent_Identity=30.8035714285714, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=271, Percent_Identity=26.1992619926199, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=193, Percent_Identity=31.6062176165803, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144160, Length=188, Percent_Identity=32.4468085106383, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=188, Percent_Identity=32.4468085106383, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=28.2407407407407, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=242, Percent_Identity=30.9917355371901, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=240, Percent_Identity=29.5833333333333, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=240, Percent_Identity=29.5833333333333, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=240, Percent_Identity=29.5833333333333, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25141363, Length=201, Percent_Identity=30.3482587064677, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=210, Percent_Identity=28.0952380952381, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=202, Percent_Identity=30.1980198019802, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=48.4126984126984, Blast_Score=230, Evalue=6e-61,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=40.650406504065, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=40.650406504065, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24650670, Length=201, Percent_Identity=36.81592039801, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=30.6513409961686, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI19920382, Length=224, Percent_Identity=30.3571428571429, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21357171, Length=195, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28571729, Length=242, Percent_Identity=28.099173553719, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI28571730, Length=242, Percent_Identity=28.099173553719, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24583077, Length=192, Percent_Identity=36.4583333333333, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583079, Length=192, Percent_Identity=36.4583333333333, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921000, Length=192, Percent_Identity=36.4583333333333, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583165, Length=169, Percent_Identity=32.5443786982249, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921018, Length=171, Percent_Identity=31.5789473684211, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24654903, Length=183, Percent_Identity=25.1366120218579, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550169, Length=194, Percent_Identity=25.2577319587629, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YNGF_BACSU (O34893)

Other databases:

- EMBL:   Y13917
- EMBL:   AL009126
- PIR:   C69893
- RefSeq:   NP_389704.1
- HSSP:   Q13825
- ProteinModelPortal:   O34893
- SMR:   O34893
- EnsemblBacteria:   EBBACT00000001831
- GeneID:   939467
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18220
- NMPDR:   fig|224308.1.peg.1825
- GenoList:   BSU18220
- GeneTree:   EBGT00070000031862
- HOGENOM:   HBG748731
- OMA:   VDYVTPH
- PhylomeDB:   O34893
- ProtClustDB:   PRK07657
- BioCyc:   BSUB:BSU18220-MONOMER
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- Gene3D:   G3DSA:1.10.12.10

Pfam domain/function: PF00378 ECH

EC number: 4.2.1.17

Molecular weight: Translated: 27873; Mature: 27742

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MGDSILFTVKNEHMALITLNRPQAANALSAEMLRNLQMIIQEIEFNSNIRCVILTGTGEK
CCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
AFCAGADLKERIKLKEDQVLESVSLIQRTAALLDALPQPVIAAINGSALGGGLELALACD
EEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEC
LRIATEAAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKEFIYTGRRVTAHEAKEIGLVE
EEEECHHHEECCCCCCEEEEECCCCHHHHHHHHCCCCCHHHEEECCEEEHHHHHHCCHHH
HVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDLATGLAIEQKAYEQTIPTK
HCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCH
DRREGLQAFQEKRRAVYKGI
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GDSILFTVKNEHMALITLNRPQAANALSAEMLRNLQMIIQEIEFNSNIRCVILTGTGEK
CCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
AFCAGADLKERIKLKEDQVLESVSLIQRTAALLDALPQPVIAAINGSALGGGLELALACD
EEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEC
LRIATEAAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKEFIYTGRRVTAHEAKEIGLVE
EEEECHHHEECCCCCCEEEEECCCCHHHHHHHHCCCCCHHHEEECCEEEHHHHHHCCHHH
HVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDLATGLAIEQKAYEQTIPTK
HCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCH
DRREGLQAFQEKRRAVYKGI
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377