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The map label for this gene is alsT

Identifier: 16078873

GI number: 16078873

Start: 1938925

End: 1940322

Strand: Direct

Name: alsT

Synonym: BSU18120

Alternate gene names: 16078873

Gene position: 1938925-1940322 (Clockwise)

Preceding gene: 255767425

Following gene: 50812243

Centisome position: 45.99

GC content: 42.7

Gene sequence:

>1398_bases
ATGGAGTCTTTTTTCAATAGTTTGATTAATATTCCAAGTGATTTCATCTGGAAATACCTATTTTATATTTTAATAGGGCT
TGGATTATTTTTTACCATACGTTTTGGTTTTATCCAATTCCGTTATTTTATTGAAATGTTCAGAATAGTAGGGGAGAAGC
CGGAAGGAAATAAAGGTGTTTCATCTATGCAGGCATTCTTTATTTCGGCCGCATCCCGAGTCGGCACAGGGAATTTGACT
GGTGTAGCCTTAGCAATTGCGACAGGCGGACCAGGCGCTGTATTTTGGATGTGGGTAGTGGCTGCAGTAGGCATGGCTTC
AAGCTTTGTCGAAAGTACATTAGCACAGCTTTATAAGGTAAGAGACGGGGAGGATTTCCGCGGAGGGCCGGCCTACTATA
TTCAAAAGGGTCTTGGTGCCAGATGGCTTGGCATCGTTTTTGCAATCTTAATTACCGTCTCATTCGGCTTGATTTTTAAC
GCTGTTCAAACAAATACAATTGCTGGAGCATTGGATGGCGCATTCCATGTAAATAAAATAGTTGTAGCCATAGTTCTGGC
GGTTTTAACTGCGTTTATCATTTTCGGCGGTTTAAAACGTGTTGTCGCTGTTTCACAGCTAATTGTGCCGGTTATGGCAG
GCATTTATATTCTTATCGCTTTATTTGTTGTCATCACGAATATTACGGCTTTCCCTGGCGTTATCGCTACAATTGTTAAA
AATGCTTTAGGTTTTGAACAAGTCGTCGGCGGCGGAATAGGCGGCATCATCGTTATCGGTGCGCAACGCGGACTTTTTTC
AAACGAAGCAGGAATGGGGAGCGCACCAAACGCGGCTGCGACGGCTCATGTATCCCATCCGGCAAAGCAAGGCTTTATTC
AAACATTAGGCGTATTTTTCGATACATTTATCATATGTACGTCCACAGCATTTATTATTTTGCTGTACAGTGTAACGCCA
AAAGGCGACGGCATCCAAGTCACACAGGCTGCTCTTAACCATCACATTGGAGGCTGGGCGCCGACTTTCATCGCAGTCGC
AATGTTCTTGTTTGCATTCAGTTCAGTTGTCGGCAACTATTATTATGGCGAGACAAACATTGAATTTATTAAAACAAGCA
AAACATGGCTGAACATTTACCGTATCGCTGTTATTGCTATGGTTGTGTATGGATCTTTATCAGGCTTCCAAATCGTTTGG
GATATGGCGGACCTCTTTATGGGTATCATGGCGCTGATCAACTTAATTGTGATTGCGCTGCTGTCAAACGTTGCTTACAA
AGTGTATAAAGATTACGCGAAACAGCGTAAGCAAGGACTTGATCCTGTGTTTAAAGCGAAAAACATCCCAGGGCTGAAAA
ACGCTGAAACATGGGAAGATGAGAAACAAGAAGCATAA

Upstream 100 bases:

>100_bases
TTCCTAATTCGTCATATTGTGATATAATAAAACTCGTTATGTTAAAAAATCTAACATCAAAATCGAATTCGTATTGAATT
GATGAAAACGGGAGGTAAAT

Downstream 100 bases:

>100_bases
TATAAATATAAAACAAAGCTGCATTCAATAGTTGAATGCAGCTTTTTCATTATTGGAAATAAATTTAATTTTTCGACTCG
AATGAAAAATGACGTGTAAA

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MESFFNSLINIPSDFIWKYLFYILIGLGLFFTIRFGFIQFRYFIEMFRIVGEKPEGNKGVSSMQAFFISAASRVGTGNLT
GVALAIATGGPGAVFWMWVVAAVGMASSFVESTLAQLYKVRDGEDFRGGPAYYIQKGLGARWLGIVFAILITVSFGLIFN
AVQTNTIAGALDGAFHVNKIVVAIVLAVLTAFIIFGGLKRVVAVSQLIVPVMAGIYILIALFVVITNITAFPGVIATIVK
NALGFEQVVGGGIGGIIVIGAQRGLFSNEAGMGSAPNAAATAHVSHPAKQGFIQTLGVFFDTFIICTSTAFIILLYSVTP
KGDGIQVTQAALNHHIGGWAPTFIAVAMFLFAFSSVVGNYYYGETNIEFIKTSKTWLNIYRIAVIAMVVYGSLSGFQIVW
DMADLFMGIMALINLIVIALLSNVAYKVYKDYAKQRKQGLDPVFKAKNIPGLKNAETWEDEKQEA

Sequences:

>Translated_465_residues
MESFFNSLINIPSDFIWKYLFYILIGLGLFFTIRFGFIQFRYFIEMFRIVGEKPEGNKGVSSMQAFFISAASRVGTGNLT
GVALAIATGGPGAVFWMWVVAAVGMASSFVESTLAQLYKVRDGEDFRGGPAYYIQKGLGARWLGIVFAILITVSFGLIFN
AVQTNTIAGALDGAFHVNKIVVAIVLAVLTAFIIFGGLKRVVAVSQLIVPVMAGIYILIALFVVITNITAFPGVIATIVK
NALGFEQVVGGGIGGIIVIGAQRGLFSNEAGMGSAPNAAATAHVSHPAKQGFIQTLGVFFDTFIICTSTAFIILLYSVTP
KGDGIQVTQAALNHHIGGWAPTFIAVAMFLFAFSSVVGNYYYGETNIEFIKTSKTWLNIYRIAVIAMVVYGSLSGFQIVW
DMADLFMGIMALINLIVIALLSNVAYKVYKDYAKQRKQGLDPVFKAKNIPGLKNAETWEDEKQEA
>Mature_465_residues
MESFFNSLINIPSDFIWKYLFYILIGLGLFFTIRFGFIQFRYFIEMFRIVGEKPEGNKGVSSMQAFFISAASRVGTGNLT
GVALAIATGGPGAVFWMWVVAAVGMASSFVESTLAQLYKVRDGEDFRGGPAYYIQKGLGARWLGIVFAILITVSFGLIFN
AVQTNTIAGALDGAFHVNKIVVAIVLAVLTAFIIFGGLKRVVAVSQLIVPVMAGIYILIALFVVITNITAFPGVIATIVK
NALGFEQVVGGGIGGIIVIGAQRGLFSNEAGMGSAPNAAATAHVSHPAKQGFIQTLGVFFDTFIICTSTAFIILLYSVTP
KGDGIQVTQAALNHHIGGWAPTFIAVAMFLFAFSSVVGNYYYGETNIEFIKTSKTWLNIYRIAVIAMVVYGSLSGFQIVW
DMADLFMGIMALINLIVIALLSNVAYKVYKDYAKQRKQGLDPVFKAKNIPGLKNAETWEDEKQEA

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family

Homologues:

Organism=Escherichia coli, GI1786188, Length=483, Percent_Identity=42.6501035196687, Blast_Score=348, Evalue=4e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALST_BACSU (Q45068)

Other databases:

- EMBL:   Z73234
- EMBL:   AL009126
- EMBL:   X67044
- PIR:   A69585
- RefSeq:   NP_389694.1
- ProteinModelPortal:   Q45068
- EnsemblBacteria:   EBBACT00000003712
- GeneID:   938191
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18120
- NMPDR:   fig|224308.1.peg.1815
- GenoList:   BSU18120
- GeneTree:   EBGT00070000031806
- HOGENOM:   HBG671706
- OMA:   LADITMA
- PhylomeDB:   Q45068
- ProtClustDB:   CLSK887361
- BioCyc:   BSUB:BSU18120-MONOMER
- InterPro:   IPR001463
- PRINTS:   PR00175
- TIGRFAMs:   TIGR00835

Pfam domain/function: PF01235 Na_Ala_symp

EC number: NA

Molecular weight: Translated: 50301; Mature: 50301

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf368bc0)-; HASH(0xf355c30)-; HASH(0xee03d3c)-; HASH(0xf1a085c)-; HASH(0xf1b47f0)-; HASH(0xf4e9844)-; HASH(0xded0ac0)-; HASH(0xf1348b8)-; HASH(0xe9c437c)-; HASH(0xe22d6d8)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESFFNSLINIPSDFIWKYLFYILIGLGLFFTIRFGFIQFRYFIEMFRIVGEKPEGNKGV
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
SSMQAFFISAASRVGTGNLTGVALAIATGGPGAVFWMWVVAAVGMASSFVESTLAQLYKV
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDGEDFRGGPAYYIQKGLGARWLGIVFAILITVSFGLIFNAVQTNTIAGALDGAFHVNKI
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VVAIVLAVLTAFIIFGGLKRVVAVSQLIVPVMAGIYILIALFVVITNITAFPGVIATIVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALGFEQVVGGGIGGIIVIGAQRGLFSNEAGMGSAPNAAATAHVSHPAKQGFIQTLGVFF
HHHCHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHH
DTFIICTSTAFIILLYSVTPKGDGIQVTQAALNHHIGGWAPTFIAVAMFLFAFSSVVGNY
HHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
YYGETNIEFIKTSKTWLNIYRIAVIAMVVYGSLSGFQIVWDMADLFMGIMALINLIVIAL
EECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSNVAYKVYKDYAKQRKQGLDPVFKAKNIPGLKNAETWEDEKQEA
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHCCCH
>Mature Secondary Structure
MESFFNSLINIPSDFIWKYLFYILIGLGLFFTIRFGFIQFRYFIEMFRIVGEKPEGNKGV
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
SSMQAFFISAASRVGTGNLTGVALAIATGGPGAVFWMWVVAAVGMASSFVESTLAQLYKV
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDGEDFRGGPAYYIQKGLGARWLGIVFAILITVSFGLIFNAVQTNTIAGALDGAFHVNKI
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VVAIVLAVLTAFIIFGGLKRVVAVSQLIVPVMAGIYILIALFVVITNITAFPGVIATIVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALGFEQVVGGGIGGIIVIGAQRGLFSNEAGMGSAPNAAATAHVSHPAKQGFIQTLGVFF
HHHCHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHH
DTFIICTSTAFIILLYSVTPKGDGIQVTQAALNHHIGGWAPTFIAVAMFLFAFSSVVGNY
HHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
YYGETNIEFIKTSKTWLNIYRIAVIAMVVYGSLSGFQIVWDMADLFMGIMALINLIVIAL
EECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSNVAYKVYKDYAKQRKQGLDPVFKAKNIPGLKNAETWEDEKQEA
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280