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The map label for this gene is yncF

Identifier: 16078829

GI number: 16078829

Start: 1900080

End: 1900514

Strand: Direct

Name: yncF

Synonym: BSU17660

Alternate gene names: 16078829

Gene position: 1900080-1900514 (Clockwise)

Preceding gene: 255767419

Following gene: 16078831

Centisome position: 45.07

GC content: 40.46

Gene sequence:

>435_bases
ATGACAATGCAAATTAAAATCAAGTATCTAGATGAAACACAAACAAGAATCAGCAAAATTGAGCAGGGAGACTGGATTGA
TCTTCGGGCAGCTGAAGATGTAACAATCAAAAAAGATGAATTTAAGCTTGTCCCATTAGGTGTTGCAATGGAGCTGCCTG
AAGGTTACGAAGCACATGTCGTCCCTCGTTCAAGTACATATAAGAACTTTGGCGTTATTCAAACAAATTCAATGGGTGTT
ATCGATGAGTCATATAAGGGAGACAACGATTTCTGGTTCTTTCCTGCTTATGCATTGCGTGATACTGAAATTAAGAAGGG
TGATCGTATCTGCCAGTTTAGAATCATGAAGAAAATGCCGGCGGTTGAATTGGTTGAAGTCGAGCATTTGGGAAACGAGG
ATAGAGGAGGACTCGGTTCAACAGGTACGAAGTAA

Upstream 100 bases:

>100_bases
TACAGCATGATTTTGAAGACGTTCAATTTATAACTGATGAAAATGACACAAGAACAAGCCTTACTGTATGATACTCAATA
AAAATTAAAGGAGCTAGTAC

Downstream 100 bases:

>100_bases
ACAAAATAAATCCTTGGCTGTCTTCATGCTGTGTGATTGATCAATGGAGGTTTCAGCCAAGGTACATATAAATATTATGT
ATAAAATGAATATTTTAATA

Product: deoxyuridine 5'-triphosphate pyrophosphatase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MTMQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGV
IDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMPAVELVEVEHLGNEDRGGLGSTGTK

Sequences:

>Translated_144_residues
MTMQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGV
IDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMPAVELVEVEHLGNEDRGGLGSTGTK
>Mature_143_residues
TMQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVI
DESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMPAVELVEVEHLGNEDRGGLGSTGTK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Caenorhabditis elegans, GI71988561, Length=144, Percent_Identity=36.8055555555556, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583610, Length=122, Percent_Identity=38.5245901639344, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921126, Length=122, Percent_Identity=38.5245901639344, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YNCF_BACSU (O31801)

Other databases:

- EMBL:   AL009126
- PIR:   H69888
- RefSeq:   NP_389649.1
- PDB:   2XCD
- PDB:   2XCE
- PDBsum:   2XCD
- PDBsum:   2XCE
- ProteinModelPortal:   O31801
- SMR:   O31801
- EnsemblBacteria:   EBBACT00000001531
- GeneID:   939541
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17660
- NMPDR:   fig|224308.1.peg.1770
- GenoList:   BSU17660
- GeneTree:   EBGT00050000002488
- HOGENOM:   HBG436079
- OMA:   PRSSTFK
- ProtClustDB:   CLSK887343
- BioCyc:   BSUB:BSU17660-MONOMER
- InterPro:   IPR008180

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16410; Mature: 16279

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: BINDING 76-76

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHV
CEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCEEEECCCEEEEEEEEEEECCCCCEEEE
VPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMP
EECCCCCCCCCEEEECCCCEEECCCCCCCCEEEECCEEEECCCCCCCCCEEEEEEHHHCC
AVELVEVEHLGNEDRGGLGSTGTK
CEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHV
EEEEEEEECCCHHHHHHHCCCCCEEEEEECCCEEEECCCEEEEEEEEEEECCCCCEEEE
VPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMP
EECCCCCCCCCEEEECCCCEEECCCCCCCCEEEECCEEEECCCCCCCCCEEEEEEHHHCC
AVELVEVEHLGNEDRGGLGSTGTK
CEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377