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The map label for this gene is ebrA

Identifier: 16078793

GI number: 16078793

Start: 1865058

End: 1865375

Strand: Reverse

Name: ebrA

Synonym: BSU17300

Alternate gene names: 16078793

Gene position: 1865375-1865058 (Counterclockwise)

Preceding gene: 255767404

Following gene: 16078792

Centisome position: 44.25

GC content: 45.6

Gene sequence:

>318_bases
ATGTTGATAGGATATATATTCCTCACGATTGCCATATGTTCGGAATCGATAGGAGCAGCCATGCTGAAAGTATCTGATGG
CTTCAAAAAGTGGAAGCCAAGTGCTTTGGTCGTTATTGGCTATTCCCTCGCCTTTTATATGCTGTCCCTGACTTTAAACC
ATATTCCCCTGAGCCTCTCCTATGCGACTTGGAGCGGGGCAGGCACGGTGCTGACGACAGTGATCGGAGTAAAATGGTTC
AAGGAAGATCTGAATGCCAAAGGGCTTATCGGGATTCTTTTATTACTATCAGGGGTCGTGTTACTTAATTGGCCGTAA

Upstream 100 bases:

>100_bases
GTGTGAAAATGAATCTCTTGACTCATACATAGGCATACACTAATATTTTTCTAGCTGAAGTTTTTTTTCTAATCTAAAAA
TATAGTGAGGAGAATTCATG

Downstream 100 bases:

>100_bases
AGGAGTGATGAAGATGAGAGGATTGCTTTATTTGGCTCTCGCCATTGTATCTGAAGTGTTTGGGAGCACGATGTTGAAGC
TTTCAGAAGGATTTACACAA

Product: small multidrug resistance efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 105; Mature: 105

Protein sequence:

>105_residues
MLIGYIFLTIAICSESIGAAMLKVSDGFKKWKPSALVVIGYSLAFYMLSLTLNHIPLSLSYATWSGAGTVLTTVIGVKWF
KEDLNAKGLIGILLLLSGVVLLNWP

Sequences:

>Translated_105_residues
MLIGYIFLTIAICSESIGAAMLKVSDGFKKWKPSALVVIGYSLAFYMLSLTLNHIPLSLSYATWSGAGTVLTTVIGVKWF
KEDLNAKGLIGILLLLSGVVLLNWP
>Mature_105_residues
MLIGYIFLTIAICSESIGAAMLKVSDGFKKWKPSALVVIGYSLAFYMLSLTLNHIPLSLSYATWSGAGTVLTTVIGVKWF
KEDLNAKGLIGILLLLSGVVLLNWP

Specific function: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family. EbrA/ebrB (TC 2.A.7.1.6) subfamily

Homologues:

Organism=Escherichia coli, GI1786755, Length=99, Percent_Identity=38.3838383838384, Blast_Score=77, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EBRA_BACSU (O31792)

Other databases:

- EMBL:   AB029306
- EMBL:   AL009126
- PIR:   D69619
- RefSeq:   NP_389612.1
- ProteinModelPortal:   O31792
- EnsemblBacteria:   EBBACT00000002689
- GeneID:   940059
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17300
- NMPDR:   fig|224308.1.peg.1734
- GenoList:   BSU17300
- GeneTree:   EBGT00050000001580
- HOGENOM:   HBG740972
- OMA:   LTRAMGY
- PhylomeDB:   O31792
- ProtClustDB:   CLSK887329
- BioCyc:   BSUB:BSU17300-MONOMER
- GO:   GO:0006810
- InterPro:   IPR000390

Pfam domain/function: PF00893 Multi_Drug_Res

EC number: NA

Molecular weight: Translated: 11349; Mature: 11349

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd876aa4)-; HASH(0xb4d1094)-; HASH(0xf2a7af4)-; HASH(0xf34a808)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYIFLTIAICSESIGAAMLKVSDGFKKWKPSALVVIGYSLAFYMLSLTLNHIPLSLS
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEE
YATWSGAGTVLTTVIGVKWFKEDLNAKGLIGILLLLSGVVLLNWP
EECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECH
>Mature Secondary Structure
MLIGYIFLTIAICSESIGAAMLKVSDGFKKWKPSALVVIGYSLAFYMLSLTLNHIPLSLS
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEE
YATWSGAGTVLTTVIGVKWFKEDLNAKGLIGILLLLSGVVLLNWP
EECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10735876; 9384377