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The map label for this gene is ebrB

Identifier: 16078792

GI number: 16078792

Start: 1864691

End: 1865044

Strand: Reverse

Name: ebrB

Synonym: BSU17290

Alternate gene names: 16078792

Gene position: 1865044-1864691 (Counterclockwise)

Preceding gene: 16078793

Following gene: 16078789

Centisome position: 44.24

GC content: 44.92

Gene sequence:

>354_bases
ATGAGAGGATTGCTTTATTTGGCTCTCGCCATTGTATCTGAAGTGTTTGGGAGCACGATGTTGAAGCTTTCAGAAGGATT
TACACAAGCCTGGCCCATTGCCGGAGTCATAGTCGGATTTCTTTCTGCTTTTACGTTTCTAAGCTTTTCTTTAAAAACAA
TTGACTTATCAAGCGCGTATGCAACATGGTCGGGCGTTGGTACCGCGCTTACAGCAATTGTCGGGTTTTTGCTTTTTGGC
GAAACCATCAGTTTAAAAGGTGTATTCGGTCTCACGCTTGTCATTGCAGGCGTGGTCGTGCTAAATCAATCGAAAGCCCA
CGCTGAAGATAAAAAACAGACGGCCTGTGAGTGA

Upstream 100 bases:

>100_bases
AAATGGTTCAAGGAAGATCTGAATGCCAAAGGGCTTATCGGGATTCTTTTATTACTATCAGGGGTCGTGTTACTTAATTG
GCCGTAAAGGAGTGATGAAG

Downstream 100 bases:

>100_bases
CCGTCTGTTTTCTTTATTCTCCACCCAGTTTCACAGAGGCTTCAAGCTGCAGCTCGACATTTCCTTGAATTGCACGCGAA
ATCATGCATGACGACTCCGC

Product: small multidrug efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MRGLLYLALAIVSEVFGSTMLKLSEGFTQAWPIAGVIVGFLSAFTFLSFSLKTIDLSSAYATWSGVGTALTAIVGFLLFG
ETISLKGVFGLTLVIAGVVVLNQSKAHAEDKKQTACE

Sequences:

>Translated_117_residues
MRGLLYLALAIVSEVFGSTMLKLSEGFTQAWPIAGVIVGFLSAFTFLSFSLKTIDLSSAYATWSGVGTALTAIVGFLLFG
ETISLKGVFGLTLVIAGVVVLNQSKAHAEDKKQTACE
>Mature_117_residues
MRGLLYLALAIVSEVFGSTMLKLSEGFTQAWPIAGVIVGFLSAFTFLSFSLKTIDLSSAYATWSGVGTALTAIVGFLLFG
ETISLKGVFGLTLVIAGVVVLNQSKAHAEDKKQTACE

Specific function: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family. EbrA/ebrB (TC 2.A.7.1.6) subfamily

Homologues:

Organism=Escherichia coli, GI1786755, Length=110, Percent_Identity=39.0909090909091, Blast_Score=87, Evalue=4e-19,
Organism=Escherichia coli, GI1787883, Length=97, Percent_Identity=42.2680412371134, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EBRB_BACSU (O31791)

Other databases:

- EMBL:   AB029306
- EMBL:   AL009126
- PIR:   E69619
- RefSeq:   NP_389611.1
- ProteinModelPortal:   O31791
- EnsemblBacteria:   EBBACT00000000478
- GeneID:   940051
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17290
- NMPDR:   fig|224308.1.peg.1733
- GenoList:   BSU17290
- GeneTree:   EBGT00050000001580
- HOGENOM:   HBG740972
- OMA:   MEVMATS
- PhylomeDB:   O31791
- ProtClustDB:   CLSK887328
- BioCyc:   BSUB:BSU17290-MONOMER
- GO:   GO:0006810
- InterPro:   IPR000390

Pfam domain/function: PF00893 Multi_Drug_Res

EC number: NA

Molecular weight: Translated: 12325; Mature: 12325

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xee4d340)-; HASH(0xf687674)-; HASH(0xf6bae48)-; HASH(0xf35b478)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLLYLALAIVSEVFGSTMLKLSEGFTQAWPIAGVIVGFLSAFTFLSFSLKTIDLSSAY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHH
ATWSGVGTALTAIVGFLLFGETISLKGVFGLTLVIAGVVVLNQSKAHAEDKKQTACE
HHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
>Mature Secondary Structure
MRGLLYLALAIVSEVFGSTMLKLSEGFTQAWPIAGVIVGFLSAFTFLSFSLKTIDLSSAY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHH
ATWSGVGTALTAIVGFLLFGETISLKGVFGLTLVIAGVVVLNQSKAHAEDKKQTACE
HHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10735876; 9384377