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The map label for this gene is aprX

Identifier: 16078789

GI number: 16078789

Start: 1861384

End: 1862712

Strand: Reverse

Name: aprX

Synonym: BSU17260

Alternate gene names: 16078789

Gene position: 1862712-1861384 (Counterclockwise)

Preceding gene: 16078792

Following gene: 255767403

Centisome position: 44.19

GC content: 47.4

Gene sequence:

>1329_bases
ATGTTTGGGTACTCTATGGTACAAATGGTGAGAGCAAATGCTCACAAGCTAGATTGGCCGTTACGGGAAACCGTATTGCA
ACTTTACAAGCCCTTCAAATGGACTCCTTGCTTCTTGCATAAATTTTTCGAAACCAAATTGCAAAACAGAAAAAAAATGT
CAGTCATTATCGAATTTGAGGAAGGCTGTCACGAGACCGGCTTTCAAATGGCTGGAGAGGTGCTGCAAAAAGAAAAACGC
AGCAAGCTAAAAAGCCGCTTCAACAAAATCAACTGCTGCAGCGCCGAGGTTACTCCTTCAGCATTACATTCCCTTCTCTC
TGAATGCAGCAATATCCGTAAAGTGTATTTAAACCGCGAAGTCAAAGCGCTTTTAGACACGGCAACCGAAGCAAGCCATG
CGAAGGAAGTCGTCAGAAACGGGCAAACCCTGACAGGAAAAGGTGTTACTGTCGCTGTTGTCGATACAGGAATCTACCCG
CATCCTGATCTAGAAGGAAGAATTATCGGATTCGCGGACATGGTCAATCAAAAAACAGAGCCGTACGATGATAATGGTCA
TGGCACACACTGTGCAGGTGATGTCGCAAGCAGCGGTGCATCCTCTTCCGGTCAATACCGCGGACCTGCACCGGAAGCCA
ATCTCATCGGCGTAAAGGTATTAAACAAGCAGGGTTCAGGAACGTTAGCAGATATTATAGAGGGTGTTGAGTGGTGTATT
CAATACAATGAGGACAACCCCGATGAACCGATTGATATTATGAGCATGTCGCTCGGGGGCGATGCCCTTAGATATGATCA
TGAACAGGAAGATCCGTTAGTCAGAGCGGTTGAGGAAGCGTGGAGCGCAGGGATTGTCGTCTGTGTCGCTGCCGGAAACT
CAGGCCCTGATTCGCAGACCATTGCAAGCCCGGGCGTGAGCGAAAAGGTGATTACAGTAGGTGCGCTTGACGATAACAAT
ACTGCAAGCAGCGATGATGATACTGTCGCTTCCTTCTCAAGCCGCGGCCCGACAGTATACGGGAAAGAAAAGCCCGATAT
TTTAGCGCCGGGTGTTAATATTATTTCCCTTCGCTCTCCGAATTCTTATATTGATAAGCTCCAAAAGTCAAGCCGTGTAG
GGTCTCAGTACTTTACAATGTCAGGAACCTCGATGGCAACACCGATTTGCGCAGGAATCGCAGCTTTAATTCTTCAGCAA
AACCCGGATCTTACCCCTGATGAAGTAAAAGAGCTTTTGAAAAACGGCACGGACAAATGGAAAGATGAAGATCCGAATAT
CTACGGAGCGGGCGCTGTCAACGCAGAAAATTCCGTTCCCGGCCAATAA

Upstream 100 bases:

>100_bases
AACAAACTCTTGTCTGTCTCTTACAGAGGACAAACAAACTTACCAAACCTATCATCTGTTCATAGAGTATACAGAGAACC
TTATAATAGGAGGAAAATAC

Downstream 100 bases:

>100_bases
ACATCATCAAAAGCCGGCAATTCTCTCTCAGCAGAACTGCCGGTTTTTTCGTTTGAATATTCATAATCATAAGGTAAAAT
AATGGAAAATAGATGAAGGA

Product: alkaline serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MFGYSMVQMVRANAHKLDWPLRETVLQLYKPFKWTPCFLHKFFETKLQNRKKMSVIIEFEEGCHETGFQMAGEVLQKEKR
SKLKSRFNKINCCSAEVTPSALHSLLSECSNIRKVYLNREVKALLDTATEASHAKEVVRNGQTLTGKGVTVAVVDTGIYP
HPDLEGRIIGFADMVNQKTEPYDDNGHGTHCAGDVASSGASSSGQYRGPAPEANLIGVKVLNKQGSGTLADIIEGVEWCI
QYNEDNPDEPIDIMSMSLGGDALRYDHEQEDPLVRAVEEAWSAGIVVCVAAGNSGPDSQTIASPGVSEKVITVGALDDNN
TASSDDDTVASFSSRGPTVYGKEKPDILAPGVNIISLRSPNSYIDKLQKSSRVGSQYFTMSGTSMATPICAGIAALILQQ
NPDLTPDEVKELLKNGTDKWKDEDPNIYGAGAVNAENSVPGQ

Sequences:

>Translated_442_residues
MFGYSMVQMVRANAHKLDWPLRETVLQLYKPFKWTPCFLHKFFETKLQNRKKMSVIIEFEEGCHETGFQMAGEVLQKEKR
SKLKSRFNKINCCSAEVTPSALHSLLSECSNIRKVYLNREVKALLDTATEASHAKEVVRNGQTLTGKGVTVAVVDTGIYP
HPDLEGRIIGFADMVNQKTEPYDDNGHGTHCAGDVASSGASSSGQYRGPAPEANLIGVKVLNKQGSGTLADIIEGVEWCI
QYNEDNPDEPIDIMSMSLGGDALRYDHEQEDPLVRAVEEAWSAGIVVCVAAGNSGPDSQTIASPGVSEKVITVGALDDNN
TASSDDDTVASFSSRGPTVYGKEKPDILAPGVNIISLRSPNSYIDKLQKSSRVGSQYFTMSGTSMATPICAGIAALILQQ
NPDLTPDEVKELLKNGTDKWKDEDPNIYGAGAVNAENSVPGQ
>Mature_442_residues
MFGYSMVQMVRANAHKLDWPLRETVLQLYKPFKWTPCFLHKFFETKLQNRKKMSVIIEFEEGCHETGFQMAGEVLQKEKR
SKLKSRFNKINCCSAEVTPSALHSLLSECSNIRKVYLNREVKALLDTATEASHAKEVVRNGQTLTGKGVTVAVVDTGIYP
HPDLEGRIIGFADMVNQKTEPYDDNGHGTHCAGDVASSGASSSGQYRGPAPEANLIGVKVLNKQGSGTLADIIEGVEWCI
QYNEDNPDEPIDIMSMSLGGDALRYDHEQEDPLVRAVEEAWSAGIVVCVAAGNSGPDSQTIASPGVSEKVITVGALDDNN
TASSDDDTVASFSSRGPTVYGKEKPDILAPGVNIISLRSPNSYIDKLQKSSRVGSQYFTMSGTSMATPICAGIAALILQQ
NPDLTPDEVKELLKNGTDKWKDEDPNIYGAGAVNAENSVPGQ

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family

Homologues:

Organism=Homo sapiens, GI4506775, Length=280, Percent_Identity=29.6428571428571, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI31317307, Length=308, Percent_Identity=29.5454545454545, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319893, Length=273, Percent_Identity=37.3626373626374, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320775, Length=286, Percent_Identity=35.3146853146853, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324576, Length=309, Percent_Identity=34.3042071197411, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI45550681, Length=319, Percent_Identity=29.153605015674, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): APRX_BACSU (O31788)

Other databases:

- EMBL:   AL009126
- PIR:   A69587
- RefSeq:   NP_389608.1
- HSSP:   Q99405
- ProteinModelPortal:   O31788
- SMR:   O31788
- MEROPS:   S08.137
- EnsemblBacteria:   EBBACT00000002946
- GeneID:   940056
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17260
- NMPDR:   fig|224308.1.peg.1730
- GenoList:   BSU17260
- GeneTree:   EBGT00050000000194
- HOGENOM:   HBG752219
- OMA:   RDPNVYG
- PhylomeDB:   O31788
- ProtClustDB:   CLSK873175
- BioCyc:   BSUB:BSU17260-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- Gene3D:   G3DSA:3.40.50.200
- PANTHER:   PTHR10795
- PRINTS:   PR00723

Pfam domain/function: PF00082 Peptidase_S8; SSF52743 Pept_S8_S53

EC number: NA

Molecular weight: Translated: 47906; Mature: 47906

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00136 SUBTILASE_ASP; PS00137 SUBTILASE_HIS; PS00138 SUBTILASE_SER

Important sites: ACT_SITE 155-155 ACT_SITE 187-187 ACT_SITE 384-384

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGYSMVQMVRANAHKLDWPLRETVLQLYKPFKWTPCFLHKFFETKLQNRKKMSVIIEFE
CCCHHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEH
EGCHETGFQMAGEVLQKEKRSKLKSRFNKINCCSAEVTPSALHSLLSECSNIRKVYLNRE
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VKALLDTATEASHAKEVVRNGQTLTGKGVTVAVVDTGIYPHPDLEGRIIGFADMVNQKTE
HHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCCCCCCCEEEEHHHHHCCCCC
PYDDNGHGTHCAGDVASSGASSSGQYRGPAPEANLIGVKVLNKQGSGTLADIIEGVEWCI
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHE
QYNEDNPDEPIDIMSMSLGGDALRYDHEQEDPLVRAVEEAWSAGIVVCVAAGNSGPDSQT
ECCCCCCCCCHHEEEECCCCCHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCC
IASPGVSEKVITVGALDDNNTASSDDDTVASFSSRGPTVYGKEKPDILAPGVNIISLRSP
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCEEECCCCCCEECCCCCEEEECCC
NSYIDKLQKSSRVGSQYFTMSGTSMATPICAGIAALILQQNPDLTPDEVKELLKNGTDKW
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
KDEDPNIYGAGAVNAENSVPGQ
CCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MFGYSMVQMVRANAHKLDWPLRETVLQLYKPFKWTPCFLHKFFETKLQNRKKMSVIIEFE
CCCHHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEH
EGCHETGFQMAGEVLQKEKRSKLKSRFNKINCCSAEVTPSALHSLLSECSNIRKVYLNRE
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VKALLDTATEASHAKEVVRNGQTLTGKGVTVAVVDTGIYPHPDLEGRIIGFADMVNQKTE
HHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCCCCCCCEEEEHHHHHCCCCC
PYDDNGHGTHCAGDVASSGASSSGQYRGPAPEANLIGVKVLNKQGSGTLADIIEGVEWCI
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHE
QYNEDNPDEPIDIMSMSLGGDALRYDHEQEDPLVRAVEEAWSAGIVVCVAAGNSGPDSQT
ECCCCCCCCCHHEEEECCCCCHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCC
IASPGVSEKVITVGALDDNNTASSDDDTVASFSSRGPTVYGKEKPDILAPGVNIISLRSP
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCEEECCCCCCEECCCCCEEEECCC
NSYIDKLQKSSRVGSQYFTMSGTSMATPICAGIAALILQQNPDLTPDEVKELLKNGTDKW
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
KDEDPNIYGAGAVNAENSVPGQ
CCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377