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The map label for this gene is pksC

Identifier: 16078772

GI number: 16078772

Start: 1783763

End: 1784629

Strand: Direct

Name: pksC

Synonym: BSU17100

Alternate gene names: 16078772

Gene position: 1783763-1784629 (Clockwise)

Preceding gene: 16078771

Following gene: 255767392

Centisome position: 42.31

GC content: 40.48

Gene sequence:

>867_bases
ATGATCACATATGTTTTTCCAGGTCAGGGTTCACAAAAACAGGGAATGGGCAGCGGCCTATTCGATGAATTTAAAGAACT
GACGGATCAGGCAGATGAGATCTTAGGATATTCTATCAAACGTCTTTGCCTGGAAAATCCTTATTCCAATTTAAACAAAA
CGCAATTTACTCAGCCGGCATTATATGTGGTAAACGCACTAAGCTATCTGAAAAAAATCAGGGATGAAGAAGTTAAACCT
GACTTTGTCGCCGGGCACAGCTTGGGTGAATACAATGCGTTATTTGCAGCTGAAGCCTTTGATTTTGAGACAGGTTTACA
GCTTGTCAGAAAAAGAGGCGAGCTGATGAGTCTGATAAGCAACGGCGGTATGGCAGCTGTTATGGGACTAAATGAAGAGC
AGGTTGCAAAGGCTCTGAAAGAATATCATTTACATGATGTTGATATCGCTAATGTGAATGCCCCATATCAAATTGTAATT
TCAGGAAAAAAAGATGAGATTGAAAAAGCCGCATCTCTTTTTGAAACGATGACTGAAGTTACAATGGTACTCCCCTTAAA
CGTAAGCGGCGCGTTTCATTCCAGATACATGAATAAAGCAAAAGAAGAATTCGAGGAGTTCCTTCATGCCTTTTATTTTT
CGCCTCCGTCTATCCCGGTTATTTCTAATGTGTATGCCAAACCGTATACATACGAATTCATGAAACAGACGCTGGCTGAT
CAAATTAATCATTCAGTGAAATGGACTGACAGCATAAGCTACTTAATGAAAAAAGGCCATATGGAGTTTGAGGAGGTAGG
GCCTGGAAATGTTTTAACTGGGTTAATTCATCGCATAAAGAAAGATGCGGAAGCAATGCCCCGCTAA

Upstream 100 bases:

>100_bases
ACCATCAACATGAACATGCTACTATTTATCCGGTCATCACTATTATAAAATCATACAGAAGTGAAACACATCTACTAATC
GGAAGGGTGGCTTTGAGAGA

Downstream 100 bases:

>100_bases
AGCATTTGCCCCTATGGCCATGTATCTCATCAATCAGGTCTAAACACAGATTTCCTTTCATTTAAGGTATAAAGTTTTAA
AGATTCAATCGTTCGATTGA

Product: malonyl-CoA-acyltransferase

Products: NA

Alternate protein names: AT

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MITYVFPGQGSQKQGMGSGLFDEFKELTDQADEILGYSIKRLCLENPYSNLNKTQFTQPALYVVNALSYLKKIRDEEVKP
DFVAGHSLGEYNALFAAEAFDFETGLQLVRKRGELMSLISNGGMAAVMGLNEEQVAKALKEYHLHDVDIANVNAPYQIVI
SGKKDEIEKAASLFETMTEVTMVLPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYTYEFMKQTLAD
QINHSVKWTDSISYLMKKGHMEFEEVGPGNVLTGLIHRIKKDAEAMPR

Sequences:

>Translated_288_residues
MITYVFPGQGSQKQGMGSGLFDEFKELTDQADEILGYSIKRLCLENPYSNLNKTQFTQPALYVVNALSYLKKIRDEEVKP
DFVAGHSLGEYNALFAAEAFDFETGLQLVRKRGELMSLISNGGMAAVMGLNEEQVAKALKEYHLHDVDIANVNAPYQIVI
SGKKDEIEKAASLFETMTEVTMVLPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYTYEFMKQTLAD
QINHSVKWTDSISYLMKKGHMEFEEVGPGNVLTGLIHRIKKDAEAMPR
>Mature_288_residues
MITYVFPGQGSQKQGMGSGLFDEFKELTDQADEILGYSIKRLCLENPYSNLNKTQFTQPALYVVNALSYLKKIRDEEVKP
DFVAGHSLGEYNALFAAEAFDFETGLQLVRKRGELMSLISNGGMAAVMGLNEEQVAKALKEYHLHDVDIANVNAPYQIVI
SGKKDEIEKAASLFETMTEVTMVLPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYTYEFMKQTLAD
QINHSVKWTDSISYLMKKGHMEFEEVGPGNVLTGLIHRIKKDAEAMPR

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the transfer of the malonyl-CoA group to the acyl-carrier-protein AcpK (Mal-AcpK)

COG id: COG0331

COG function: function code I; (acyl-carrier-protein) S-malonyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fabD family

Homologues:

Organism=Homo sapiens, GI51243059, Length=300, Percent_Identity=33.6666666666667, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1787334, Length=284, Percent_Identity=36.6197183098592, Blast_Score=164, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI71984332, Length=299, Percent_Identity=27.4247491638796, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17550940, Length=295, Percent_Identity=25.4237288135593, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665753, Length=306, Percent_Identity=31.3725490196078, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PKSC_BACSU (O34825)

Other databases:

- EMBL:   AL009126
- PIR:   A69678
- RefSeq:   NP_389591.1
- HSSP:   P0AAI9
- ProteinModelPortal:   O34825
- SMR:   O34825
- EnsemblBacteria:   EBBACT00000003808
- GeneID:   939504
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17100
- NMPDR:   fig|224308.1.peg.1712
- GenoList:   BSU17100
- GeneTree:   EBGT00050000000472
- HOGENOM:   HBG687898
- OMA:   CLENPYS
- PhylomeDB:   O34825
- ProtClustDB:   CLSK887323
- BioCyc:   BSUB:BSU17100-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR004410
- InterPro:   IPR016036
- Gene3D:   G3DSA:3.40.366.10
- TIGRFAMs:   TIGR00128

Pfam domain/function: PF00698 Acyl_transf_1; SSF52151 Acyl_Trfase/lysoPlipase; SSF55048 Malonyl_transacylase_ACP-bd

EC number: =2.3.1.39

Molecular weight: Translated: 32464; Mature: 32464

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: ACT_SITE 87-87 ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITYVFPGQGSQKQGMGSGLFDEFKELTDQADEILGYSIKRLCLENPYSNLNKTQFTQPA
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHH
LYVVNALSYLKKIRDEEVKPDFVAGHSLGEYNALFAAEAFDFETGLQLVRKRGELMSLIS
HHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
NGGMAAVMGLNEEQVAKALKEYHLHDVDIANVNAPYQIVISGKKDEIEKAASLFETMTEV
CCCEEEEECCCHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCHHHHHHHHHHHHHHHHH
TMVLPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYTYEFMKQTLAD
HHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHH
QINHSVKWTDSISYLMKKGHMEFEEVGPGNVLTGLIHRIKKDAEAMPR
HHCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITYVFPGQGSQKQGMGSGLFDEFKELTDQADEILGYSIKRLCLENPYSNLNKTQFTQPA
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHH
LYVVNALSYLKKIRDEEVKPDFVAGHSLGEYNALFAAEAFDFETGLQLVRKRGELMSLIS
HHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
NGGMAAVMGLNEEQVAKALKEYHLHDVDIANVNAPYQIVISGKKDEIEKAASLFETMTEV
CCCEEEEECCCHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCHHHHHHHHHHHHHHHHH
TMVLPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYTYEFMKQTLAD
HHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHH
QINHSVKWTDSISYLMKKGHMEFEEVGPGNVLTGLIHRIKKDAEAMPR
HHCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377