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The map label for this gene is pksB

Identifier: 16078771

GI number: 16078771

Start: 1782713

End: 1783390

Strand: Direct

Name: pksB

Synonym: BSU17090

Alternate gene names: 16078771

Gene position: 1782713-1783390 (Clockwise)

Preceding gene: 16078770

Following gene: 16078772

Centisome position: 42.29

GC content: 38.94

Gene sequence:

>678_bases
ATGAATCTTACTTACAAGGTGCATCCAATTAAAACAAGGTATCAGGGCTGGACGAATTATTGCTATATCATTGAAGATAT
TGTAAGCAGATCTGCAATTGTTGTTGATCCCTCATGGGAATTGAGTAAGATAACCACTACACTTTCCGAACTCGAAGCGG
AATTAAAGGCAGTCGCATTGACACACTCTCATTATGATCATGTAAATCTGGTAGACCCGCTGACGAAGATGTTTAACGCT
CAAGTTTATATGTCGAAAAAAGAAATTGATTATTATCAATTCCGATGCAGAAATTTAATTAGCCTAGATGATCATCAGAC
GATCAGCATAGGAAACACAAGAGCACAGTGCCTTCTCACACCGGGGCATACAGCCGGCGGAATGTGTTATTTGTTTTCTG
AGAGTATTTTTACCGGAGATACGGTGTTTACAGAAGGGTGCGGCATATGTGAGGATGATGGCAGCTCTGCGGAAGAGATG
TTTGATAGCATTCAAAGAATAAAGTCGGAGGTTTCACCCCATGTACGGGTGTATCCTGGTCACTCATTTGGCAAATCGCC
CGGCCATTCGATAAAGGATCTTTATCAGCATAATATTTACTTTCAAATTGATAAGAAAGAATATTTTGTGAAATTTCGCA
CTCGTAAAAACCAAAAAGGTATTTTTGATTTTAAATAA

Upstream 100 bases:

>100_bases
GGAAAACAATCATGCTGTTTGGTAAAGTGGAATGAAAGACGGACTGAGGGGTGAACACTGGTCCATCGTTTAAGAACTTT
ACATGATAGGGATGGTATAA

Downstream 100 bases:

>100_bases
TCCTTCTGAATCTATTATCCTATTTTTCTTCGAATATCTAAATGATTTTTATTTTCGAAAATGATTACATAGAAGGTGTT
ATTTATTTTGAAAACTCATT

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNLTYKVHPIKTRYQGWTNYCYIIEDIVSRSAIVVDPSWELSKITTTLSELEAELKAVALTHSHYDHVNLVDPLTKMFNA
QVYMSKKEIDYYQFRCRNLISLDDHQTISIGNTRAQCLLTPGHTAGGMCYLFSESIFTGDTVFTEGCGICEDDGSSAEEM
FDSIQRIKSEVSPHVRVYPGHSFGKSPGHSIKDLYQHNIYFQIDKKEYFVKFRTRKNQKGIFDFK

Sequences:

>Translated_225_residues
MNLTYKVHPIKTRYQGWTNYCYIIEDIVSRSAIVVDPSWELSKITTTLSELEAELKAVALTHSHYDHVNLVDPLTKMFNA
QVYMSKKEIDYYQFRCRNLISLDDHQTISIGNTRAQCLLTPGHTAGGMCYLFSESIFTGDTVFTEGCGICEDDGSSAEEM
FDSIQRIKSEVSPHVRVYPGHSFGKSPGHSIKDLYQHNIYFQIDKKEYFVKFRTRKNQKGIFDFK
>Mature_225_residues
MNLTYKVHPIKTRYQGWTNYCYIIEDIVSRSAIVVDPSWELSKITTTLSELEAELKAVALTHSHYDHVNLVDPLTKMFNA
QVYMSKKEIDYYQFRCRNLISLDDHQTISIGNTRAQCLLTPGHTAGGMCYLFSESIFTGDTVFTEGCGICEDDGSSAEEM
FDSIQRIKSEVSPHVRVYPGHSFGKSPGHSIKDLYQHNIYFQIDKKEYFVKFRTRKNQKGIFDFK

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily

Homologues:

Organism=Homo sapiens, GI94538320, Length=173, Percent_Identity=30.0578034682081, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI94538322, Length=173, Percent_Identity=30.0578034682081, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI46361987, Length=170, Percent_Identity=28.2352941176471, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI14150041, Length=170, Percent_Identity=28.2352941176471, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI21703352, Length=176, Percent_Identity=27.8409090909091, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI116642887, Length=176, Percent_Identity=27.8409090909091, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787158, Length=197, Percent_Identity=26.3959390862944, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320478, Length=202, Percent_Identity=28.2178217821782, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356335, Length=177, Percent_Identity=32.7683615819209, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24667711, Length=177, Percent_Identity=32.7683615819209, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667703, Length=177, Percent_Identity=32.7683615819209, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221330176, Length=206, Percent_Identity=26.6990291262136, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PKSB_BACSU (O34769)

Other databases:

- EMBL:   AL009126
- PIR:   H69677
- RefSeq:   NP_389590.1
- HSSP:   Q9SID3
- ProteinModelPortal:   O34769
- SMR:   O34769
- EnsemblBacteria:   EBBACT00000000467
- GeneID:   939445
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17090
- NMPDR:   fig|224308.1.peg.1711
- GenoList:   BSU17090
- GeneTree:   EBGT00050000000388
- HOGENOM:   HBG337999
- OMA:   NTHEAVW
- PhylomeDB:   O34769
- ProtClustDB:   CLSK887322
- BioCyc:   BSUB:BSU17090-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001279
- SMART:   SM00849

Pfam domain/function: PF00753 Lactamase_B

EC number: NA

Molecular weight: Translated: 25909; Mature: 25909

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTYKVHPIKTRYQGWTNYCYIIEDIVSRSAIVVDPSWELSKITTTLSELEAELKAVAL
CCCEEEEECCCEECCCCCCEEEEEHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEE
THSHYDHVNLVDPLTKMFNAQVYMSKKEIDYYQFRCRNLISLDDHQTISIGNTRAQCLLT
ECCCCCCCCHHHHHHHHHCCEEEEEHHCCHHHHHHHCCCCCCCCCCEEEECCCCEEEEEE
PGHTAGGMCYLFSESIFTGDTVFTEGCGICEDDGSSAEEMFDSIQRIKSEVSPHVRVYPG
CCCCCCCEEEEEECCEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECC
HSFGKSPGHSIKDLYQHNIYFQIDKKEYFVKFRTRKNQKGIFDFK
CCCCCCCCCHHHHHHHCCEEEEECCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNLTYKVHPIKTRYQGWTNYCYIIEDIVSRSAIVVDPSWELSKITTTLSELEAELKAVAL
CCCEEEEECCCEECCCCCCEEEEEHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEE
THSHYDHVNLVDPLTKMFNAQVYMSKKEIDYYQFRCRNLISLDDHQTISIGNTRAQCLLT
ECCCCCCCCHHHHHHHHHCCEEEEEHHCCHHHHHHHCCCCCCCCCCEEEECCCCEEEEEE
PGHTAGGMCYLFSESIFTGDTVFTEGCGICEDDGSSAEEMFDSIQRIKSEVSPHVRVYPG
CCCCCCCEEEEEECCEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECC
HSFGKSPGHSIKDLYQHNIYFQIDKKEYFVKFRTRKNQKGIFDFK
CCCCCCCCCHHHHHHHCCEEEEECCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377