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The map label for this gene is ymcB

Identifier: 16078764

GI number: 16078764

Start: 1772843

End: 1774372

Strand: Direct

Name: ymcB

Synonym: BSU17010

Alternate gene names: 16078764

Gene position: 1772843-1774372 (Clockwise)

Preceding gene: 16078763

Following gene: 16078765

Centisome position: 42.05

GC content: 43.07

Gene sequence:

>1530_bases
ATGAATGAAAAACAAAAATTAGAGAGCGGACAAGTTCATCCATCGGACAAAAAATCCGAGAAGGATTACAGCAAGTACTT
TGAAGCTGTTTACATTCCGCCTTCTTTAAAAGACGCGAAAAAACGAGGCAAGGAAGCCGTTACTTATCATAATGACTTTA
AAATTTCTGAACAATTTAAAGGATTGGGAGACGGAAGAAAGTTCTATATCCGTACGTACGGCTGCCAAATGAATGAACAC
GATACAGAGGTTATGGCAGGGATCTTTATGGCGCTCGGTTACGAAGCGACAAACTCTGTTGACGATGCCAATGTCATTTT
GTTAAATACATGTGCAATCCGTGAAAATGCTGAAAACAAGGTGTTTGGTGAGCTCGGGCACTTAAAAGCACTGAAAAAAA
ACAATCCCGATCTGATTTTAGGCGTGTGCGGCTGTATGTCCCAAGAGGAATCAGTCGTGAACCGTATTTTGAAAAAACAT
CCGTTTGTCGATATGATTTTCGGAACGCATAACATTCATCGCCTGCCGGAGCTTTTGTCAGAAGCATACCTTTCAAAAGA
AATGGTTGTAGAAGTTTGGTCTAAGGAAGGGGACGTTATTGAAAACCTTCCAAAAGTCCGAAATGGAAAAATTAAGGGCT
GGGTCAATATCATGTACGGCTGTGACAAATTCTGTACGTATTGCATTGTGCCTTACACACGCGGAAAAGAAAGAAGCCGC
CGCCCTGAGGATATCATTCAGGAAGTGAGAAGGCTCGCAAGTGAAGGCTATAAGGAAATTACTCTGTTAGGCCAAAACGT
AAACGCGTATGGAAAAGACTTTGAAGATATGACGTACGGACTTGGTGATTTGATGGATGAACTGAGAAAAATCGATATCC
CGAGAATCCGTTTTACAACGAGTCATCCGCGCGACTTTGACGACCGCCTCATTGAAGTGCTGGCAAAAGGCGGAAATCTG
CTTGATCACATTCATCTTCCGGTTCAATCAGGAAGCTCAGAAGTTCTCAAGCTGATGGCCCGCAAATATGACAGAGAGCG
TTACATGGAGCTGGTGCGAAAAATTAAAGAAGCAATGCCAAACGCTTCTTTGACAACGGACATTATCGTCGGATTTCCAA
ATGAAACGGACGAACAGTTTGAAGAAACGCTTTCTCTATACCGTGAAGTGGAGTTTGACAGCGCCTATACGTTCATTTAC
TCTCCGCGTGAAGGCACTCCGGCTGCTAAGATGAAAGATAATGTACCGATGCGTGTGAAAAAAGAACGCCTGCAGCGTCT
AAACGCACTGGTGAATGAAATTTCTGCTAAAAAAATGAAGGAATACGAAGGCAAGGTTGTCGAAGTATTAGTTGAGGGTG
AAAGCAAAAATAACCCTGATATTCTTGCTGGCTATACTGAAAAAAGCAAGCTTGTCAATTTCAAAGGGCCGAAGGAAGCC
ATCGGCAAAATCGTCCGCGTGAAAATCCAGCAAGCGAAAACATGGTCGCTTGACGGAGAAATGGTAGGAGAAGCAATCGA
GGTGAAATGA

Upstream 100 bases:

>100_bases
ATTCTCAATGAGTGTACAATGATAGGTTGCCAGTTGTGCAGAGCTTCAATAACCTTTATACTGAAGGGTGGAAGTTTGGT
TTGAAAGGAGAAAATATGAC

Downstream 100 bases:

>100_bases
GATGACGCTCTACTCAAAAAAAGACATTGTGCAGCAGGCTCGAAACCTTGCAAAAATGATTTCTGAAACAGAAGAGGTTG
ATTTTTTCAAACGGGCTGAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEKQKLESGQVHPSDKKSEKDYSKYFEAVYIPPSLKDAKKRGKEAVTYHNDFKISEQFKGLGDGRKFYIRTYGCQMNEH
DTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENKVFGELGHLKALKKNNPDLILGVCGCMSQEESVVNRILKKH
PFVDMIFGTHNIHRLPELLSEAYLSKEMVVEVWSKEGDVIENLPKVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKIDIPRIRFTTSHPRDFDDRLIEVLAKGGNL
LDHIHLPVQSGSSEVLKLMARKYDRERYMELVRKIKEAMPNASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIY
SPREGTPAAKMKDNVPMRVKKERLQRLNALVNEISAKKMKEYEGKVVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKEA
IGKIVRVKIQQAKTWSLDGEMVGEAIEVK

Sequences:

>Translated_509_residues
MNEKQKLESGQVHPSDKKSEKDYSKYFEAVYIPPSLKDAKKRGKEAVTYHNDFKISEQFKGLGDGRKFYIRTYGCQMNEH
DTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENKVFGELGHLKALKKNNPDLILGVCGCMSQEESVVNRILKKH
PFVDMIFGTHNIHRLPELLSEAYLSKEMVVEVWSKEGDVIENLPKVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKIDIPRIRFTTSHPRDFDDRLIEVLAKGGNL
LDHIHLPVQSGSSEVLKLMARKYDRERYMELVRKIKEAMPNASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIY
SPREGTPAAKMKDNVPMRVKKERLQRLNALVNEISAKKMKEYEGKVVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKEA
IGKIVRVKIQQAKTWSLDGEMVGEAIEVK
>Mature_509_residues
MNEKQKLESGQVHPSDKKSEKDYSKYFEAVYIPPSLKDAKKRGKEAVTYHNDFKISEQFKGLGDGRKFYIRTYGCQMNEH
DTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENKVFGELGHLKALKKNNPDLILGVCGCMSQEESVVNRILKKH
PFVDMIFGTHNIHRLPELLSEAYLSKEMVVEVWSKEGDVIENLPKVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKIDIPRIRFTTSHPRDFDDRLIEVLAKGGNL
LDHIHLPVQSGSSEVLKLMARKYDRERYMELVRKIKEAMPNASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIY
SPREGTPAAKMKDNVPMRVKKERLQRLNALVNEISAKKMKEYEGKVVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKEA
IGKIVRVKIQQAKTWSLDGEMVGEAIEVK

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=37.551867219917, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=37.551867219917, Blast_Score=304, Evalue=2e-82,
Organism=Homo sapiens, GI93277076, Length=454, Percent_Identity=30.6167400881057, Blast_Score=186, Evalue=6e-47,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=44.3181818181818, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=29.0465631929047, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=476, Percent_Identity=34.453781512605, Blast_Score=272, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=341, Percent_Identity=30.791788856305, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21356207, Length=486, Percent_Identity=36.4197530864198, Blast_Score=295, Evalue=7e-80,
Organism=Drosophila melanogaster, GI19922432, Length=455, Percent_Identity=30.1098901098901, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58171; Mature: 58171

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKQKLESGQVHPSDKKSEKDYSKYFEAVYIPPSLKDAKKRGKEAVTYHNDFKISEQFK
CCCHHHCCCCCCCCCCCCCHHHHHHHHHEEECCCCHHHHHHCCCCEEEECCCCEEHHHHC
GLGDGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENK
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCH
VFGELGHLKALKKNNPDLILGVCGCMSQEESVVNRILKKHPFVDMIFGTHNIHRLPELLS
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
EAYLSKEMVVEVWSKEGDVIENLPKVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHHHHHHHHHHCCCCCHHHHCCHHCCCCCEEEEEEHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKIDIPRIRFTT
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEC
SHPRDFDDRLIEVLAKGGNLLDHIHLPVQSGSSEVLKLMARKYDRERYMELVRKIKEAMP
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVK
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNEISAKKMKEYEGKVVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKEA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCEECCCCCHHH
IGKIVRVKIQQAKTWSLDGEMVGEAIEVK
HHHHHHHHHHHCCEECCCHHHHCCHHCCC
>Mature Secondary Structure
MNEKQKLESGQVHPSDKKSEKDYSKYFEAVYIPPSLKDAKKRGKEAVTYHNDFKISEQFK
CCCHHHCCCCCCCCCCCCCHHHHHHHHHEEECCCCHHHHHHCCCCEEEECCCCEEHHHHC
GLGDGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENK
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCH
VFGELGHLKALKKNNPDLILGVCGCMSQEESVVNRILKKHPFVDMIFGTHNIHRLPELLS
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
EAYLSKEMVVEVWSKEGDVIENLPKVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHHHHHHHHHHCCCCCHHHHCCHHCCCCCEEEEEEHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKIDIPRIRFTT
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEC
SHPRDFDDRLIEVLAKGGNLLDHIHLPVQSGSSEVLKLMARKYDRERYMELVRKIKEAMP
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVK
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNEISAKKMKEYEGKVVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKEA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCEECCCCCHHH
IGKIVRVKIQQAKTWSLDGEMVGEAIEVK
HHHHHHHHHHHCCEECCCHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA