Click here to switch to the map view.

The map label for this gene is kbl

Identifier: 16078763

GI number: 16078763

Start: 1771517

End: 1772695

Strand: Direct

Name: kbl

Synonym: BSU17000

Alternate gene names: 16078763

Gene position: 1771517-1772695 (Clockwise)

Preceding gene: 16078762

Following gene: 16078764

Centisome position: 42.02

GC content: 45.63

Gene sequence:

>1179_bases
ATGACGAAGGAATTTGAGTTTTTAAAAGCAGAGCTTAATAGTATGAAAGAAAACCATACATGGCAAGACATAAAACAGCT
TGAATCTATGCAGGGCCCATCTGTCACAGTGAATCACCAAAAAGTCATTCAGCTATCTTCTAATAATTACCTCGGATTCA
CTTCACATCCTAGACTCATCAACGCCGCACAGGAGGCCGTTCAGCAGTATGGAGCCGGCACCGGATCAGTGAGAACGATT
GCGGGTACATTTACAATGCATCAAGAGCTTGAGAAAAAGCTGGCAGCCTTTAAAAAAACGGAGGCGGCACTTGTATTCCA
ATCAGGCTTCACAACAAACCAAGGCGTACTTTCAAGTATTCTATCAAAAGAGGACATTGTCATCTCAGATGAATTGAACC
ATGCCTCTATTATTGACGGAATTCGACTGACAAAGGCGGATAAAAAGGTGTATCAGCACGTCAATATGAGTGATTTAGAG
CGGGTGCTGAGAAAGTCAATGAATTATCGGATGCGTCTGATTGTGACAGACGGCGTATTTTCCATGGATGGCAACATAGC
TCCTCTGCCTGATATTGTAGAGCTCGCTGAGAAATATGACGCATTTGTGATGGTGGATGACGCCCATGCATCCGGAGTAC
TTGGCGAAAACGGCAGGGGAACGGTGAATCACTTCGGTCTTGACGGCAGAGTGCATATTCAGGTCGGAACATTAAGCAAG
GCAATCGGAGTGCTCGGCGGCTACGCTGCAGGTTCAAAGGTGCTGATCGATTATTTGCGCCATAAAGGCCGTCCATTTTT
ATTCAGCACATCTCATCCGCCGGCAGTCACTGCAGCTTGTATGGAAGCGATTGATGTCTTGCTTGAAGAGCCGGAGCATA
TGGAGCGCTTGTGGGAGAATACTGCCTATTTTAAAGCAATGCTTGTGAAAATGGGTCTGACTCTCACGAAGAGTGAAACG
CCGATTCTTCCTATTTTAATAGGTGATGAAGGTGTGGCAAAGCAATTTTCAGATCAGCTCCTTTCTCGCGGTGTTTTTGC
CCAAAGTATCGTTTTCCCGACTGTAGCAAAGGGAAAAGCCAGAATTCGCACGATTATAACAGCAGAGCACACCAAAGATG
AACTGGATCAGGCGCTTGATGTCATCGAAAAGACGGCAAAGGAGCTCCAGCTATTGTAA

Upstream 100 bases:

>100_bases
TACCCATCAGTTTCCATTAGAGGAGTTTGAAAAAGGTTTCGAACTGATGAGAAGCGGGCAGTGCGGAAAAGTAATTTTAA
TTCCATAAAGGGGGATAAAC

Downstream 100 bases:

>100_bases
CAAGAGGAAGAGTTTACTCTTCCTCTTTTGATTCCTTATTGATTATTATTCTCAATGAGTGTACAATGATAGGTTGCCAG
TTGTGCAGAGCTTCAATAAC

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTKEFEFLKAELNSMKENHTWQDIKQLESMQGPSVTVNHQKVIQLSSNNYLGFTSHPRLINAAQEAVQQYGAGTGSVRTI
AGTFTMHQELEKKLAAFKKTEAALVFQSGFTTNQGVLSSILSKEDIVISDELNHASIIDGIRLTKADKKVYQHVNMSDLE
RVLRKSMNYRMRLIVTDGVFSMDGNIAPLPDIVELAEKYDAFVMVDDAHASGVLGENGRGTVNHFGLDGRVHIQVGTLSK
AIGVLGGYAAGSKVLIDYLRHKGRPFLFSTSHPPAVTAACMEAIDVLLEEPEHMERLWENTAYFKAMLVKMGLTLTKSET
PILPILIGDEGVAKQFSDQLLSRGVFAQSIVFPTVAKGKARIRTIITAEHTKDELDQALDVIEKTAKELQLL

Sequences:

>Translated_392_residues
MTKEFEFLKAELNSMKENHTWQDIKQLESMQGPSVTVNHQKVIQLSSNNYLGFTSHPRLINAAQEAVQQYGAGTGSVRTI
AGTFTMHQELEKKLAAFKKTEAALVFQSGFTTNQGVLSSILSKEDIVISDELNHASIIDGIRLTKADKKVYQHVNMSDLE
RVLRKSMNYRMRLIVTDGVFSMDGNIAPLPDIVELAEKYDAFVMVDDAHASGVLGENGRGTVNHFGLDGRVHIQVGTLSK
AIGVLGGYAAGSKVLIDYLRHKGRPFLFSTSHPPAVTAACMEAIDVLLEEPEHMERLWENTAYFKAMLVKMGLTLTKSET
PILPILIGDEGVAKQFSDQLLSRGVFAQSIVFPTVAKGKARIRTIITAEHTKDELDQALDVIEKTAKELQLL
>Mature_391_residues
TKEFEFLKAELNSMKENHTWQDIKQLESMQGPSVTVNHQKVIQLSSNNYLGFTSHPRLINAAQEAVQQYGAGTGSVRTIA
GTFTMHQELEKKLAAFKKTEAALVFQSGFTTNQGVLSSILSKEDIVISDELNHASIIDGIRLTKADKKVYQHVNMSDLER
VLRKSMNYRMRLIVTDGVFSMDGNIAPLPDIVELAEKYDAFVMVDDAHASGVLGENGRGTVNHFGLDGRVHIQVGTLSKA
IGVLGGYAAGSKVLIDYLRHKGRPFLFSTSHPPAVTAACMEAIDVLLEEPEHMERLWENTAYFKAMLVKMGLTLTKSETP
ILPILIGDEGVAKQFSDQLLSRGVFAQSIVFPTVAKGKARIRTIITAEHTKDELDQALDVIEKTAKELQLL

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI7657118, Length=384, Percent_Identity=40.8854166666667, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI284448556, Length=409, Percent_Identity=38.3863080684597, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI4758668, Length=368, Percent_Identity=35.5978260869565, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI83977442, Length=393, Percent_Identity=32.824427480916, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI83977440, Length=393, Percent_Identity=32.824427480916, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI83977444, Length=393, Percent_Identity=32.824427480916, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI119220554, Length=370, Percent_Identity=32.972972972973, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4502025, Length=398, Percent_Identity=30.6532663316583, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI40316939, Length=398, Percent_Identity=30.6532663316583, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI5454084, Length=379, Percent_Identity=30.3430079155673, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1790046, Length=392, Percent_Identity=41.0714285714286, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1786993, Length=353, Percent_Identity=34.2776203966006, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71994529, Length=397, Percent_Identity=42.3173803526448, Blast_Score=330, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI17560912, Length=359, Percent_Identity=32.033426183844, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17560914, Length=359, Percent_Identity=32.033426183844, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI32566772, Length=371, Percent_Identity=30.9973045822102, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI71982617, Length=397, Percent_Identity=26.1964735516373, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71982625, Length=397, Percent_Identity=26.1964735516373, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320438, Length=376, Percent_Identity=35.1063829787234, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6320267, Length=368, Percent_Identity=32.6086956521739, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323954, Length=281, Percent_Identity=26.3345195729537, Blast_Score=122, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24662918, Length=390, Percent_Identity=44.1025641025641, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI17136286, Length=375, Percent_Identity=35.2, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI17137420, Length=398, Percent_Identity=31.4070351758794, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24653280, Length=394, Percent_Identity=26.3959390862944, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24653276, Length=394, Percent_Identity=26.3959390862944, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24653278, Length=394, Percent_Identity=26.3959390862944, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIKB_BACSU (O31777)

Other databases:

- EMBL:   AL009126
- PIR:   G69647
- RefSeq:   NP_389582.1
- ProteinModelPortal:   O31777
- SMR:   O31777
- EnsemblBacteria:   EBBACT00000002770
- GeneID:   939651
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17000
- NMPDR:   fig|224308.1.peg.1703
- GenoList:   BSU17000
- GeneTree:   EBGT00050000000589
- HOGENOM:   HBG649839
- OMA:   TAGHTKE
- PhylomeDB:   O31777
- ProtClustDB:   PRK06939
- BioCyc:   BSUB:BSU17000-MONOMER
- BRENDA:   2.3.1.29
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR010962
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR13693:SF15
- TIGRFAMs:   TIGR01825

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.29; =2.3.1.47

Molecular weight: Translated: 43284; Mature: 43153

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 134-134 BINDING 181-181 BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEFEFLKAELNSMKENHTWQDIKQLESMQGPSVTVNHQKVIQLSSNNYLGFTSHPRLI
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCEEEEECCCCEEEECCCCHHH
NAAQEAVQQYGAGTGSVRTIAGTFTMHQELEKKLAAFKKTEAALVFQSGFTTNQGVLSSI
HHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
LSKEDIVISDELNHASIIDGIRLTKADKKVYQHVNMSDLERVLRKSMNYRMRLIVTDGVF
HCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCEE
SMDGNIAPLPDIVELAEKYDAFVMVDDAHASGVLGENGRGTVNHFGLDGRVHIQVGTLSK
ECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEEECCHHH
AIGVLGGYAAGSKVLIDYLRHKGRPFLFSTSHPPAVTAACMEAIDVLLEEPEHMERLWEN
HHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
TAYFKAMLVKMGLTLTKSETPILPILIGDEGVAKQFSDQLLSRGVFAQSIVFPTVAKGKA
HHHHHHHHHHHCCEEECCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCHHCCCCC
RIRTIITAEHTKDELDQALDVIEKTAKELQLL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKEFEFLKAELNSMKENHTWQDIKQLESMQGPSVTVNHQKVIQLSSNNYLGFTSHPRLI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCEEEEECCCCEEEECCCCHHH
NAAQEAVQQYGAGTGSVRTIAGTFTMHQELEKKLAAFKKTEAALVFQSGFTTNQGVLSSI
HHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
LSKEDIVISDELNHASIIDGIRLTKADKKVYQHVNMSDLERVLRKSMNYRMRLIVTDGVF
HCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCEE
SMDGNIAPLPDIVELAEKYDAFVMVDDAHASGVLGENGRGTVNHFGLDGRVHIQVGTLSK
ECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEEECCHHH
AIGVLGGYAAGSKVLIDYLRHKGRPFLFSTSHPPAVTAACMEAIDVLLEEPEHMERLWEN
HHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
TAYFKAMLVKMGLTLTKSETPILPILIGDEGVAKQFSDQLLSRGVFAQSIVFPTVAKGKA
HHHHHHHHHHHCCEEECCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCHHCCCCC
RIRTIITAEHTKDELDQALDVIEKTAKELQLL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377