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The map label for this gene is pyrK

Identifier: 16078617

GI number: 16078617

Start: 1626948

End: 1627718

Strand: Direct

Name: pyrK

Synonym: BSU15530

Alternate gene names: 16078617

Gene position: 1626948-1627718 (Clockwise)

Preceding gene: 16078616

Following gene: 16078618

Centisome position: 38.59

GC content: 48.12

Gene sequence:

>771_bases
ATGAAAAAAGCGTATTTGACAGTATGTTCTAACCAGCAAATTGCAGACCGGGTGTTTCAAATGGTTCTGAAAGGGGAGCT
TGTCCAAGGGTTTACAACCCCTGGACAGTTCCTTCATCTTAAAGTGAGCGAAGCGGTTACGCCTCTTTTGAGAAGGCCGA
TCAGCATCGCAGACGTCAACTTTGAAAAAAATGAAGTCACCATCATTTATCGGGTAGATGGGGAAGGGACAAGACTCTTG
TCACTGAAGCAGCAGGGAGAACTTGTGGATGTCCTCGGGCCTTTGGGAAATGGCTTTCCTGTTAATGAAGTTCAACCCGG
AAAGACGGCTTTGCTGGTAGGAGGCGGAGTAGGTGTGCCGCCTCTCCAAGAGCTGTCGAAACGCTTGATTGAAAAAGGGG
TAAATGTCATCCACGTTTTAGGATTCCAATCGGCAAAGGATGTTTTTTACGAGGAAGAATGCCGGCAGTACGGAGACACG
TATGTGGCAACAGCTGACGGAAGCTACGGGGAAACCGGATTTGTCACAGATGTGATTAAACGGAAAAAGCTAGAGTTTGA
TATCCTCCTCAGCTGCGGGCCGACACCGATGCTCAAGGCGTTAAAACAGGAATATGCCCATAAAGAAGTATATCTGTCCA
TGGAGGAACGAATGGGCTGCGGAATCGGCGCATGCTTCGCGTGTGTGTGCCATACAAACGAAAGTGAGACATCCTATGTA
AAAGTATGTCTCGACGGGCCTGTATTTAAAGCTCAGGAGGTGGCGCTGTAA

Upstream 100 bases:

>100_bases
TAGATACGGCAGAGGCGATATTGCGAGTGCTGGAAAGCATGACATTCCGTGCTGATCAAATGCCGGCAGTCAACACAAAT
CAGGAGGCGGCAGTCACTAT

Downstream 100 bases:

>100_bases
TGCTAGAGGTGAAATTGCCGGGACTTGATTTGAAAAACCCAATCATTCCTGCATCAGGCTGCTTCGGTTTTGGAAAAGAA
TTTTCACGTTTTTATGATTT

Product: dihydroorotate dehydrogenase electron transfer subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKKAYLTVCSNQQIADRVFQMVLKGELVQGFTTPGQFLHLKVSEAVTPLLRRPISIADVNFEKNEVTIIYRVDGEGTRLL
SLKQQGELVDVLGPLGNGFPVNEVQPGKTALLVGGGVGVPPLQELSKRLIEKGVNVIHVLGFQSAKDVFYEEECRQYGDT
YVATADGSYGETGFVTDVIKRKKLEFDILLSCGPTPMLKALKQEYAHKEVYLSMEERMGCGIGACFACVCHTNESETSYV
KVCLDGPVFKAQEVAL

Sequences:

>Translated_256_residues
MKKAYLTVCSNQQIADRVFQMVLKGELVQGFTTPGQFLHLKVSEAVTPLLRRPISIADVNFEKNEVTIIYRVDGEGTRLL
SLKQQGELVDVLGPLGNGFPVNEVQPGKTALLVGGGVGVPPLQELSKRLIEKGVNVIHVLGFQSAKDVFYEEECRQYGDT
YVATADGSYGETGFVTDVIKRKKLEFDILLSCGPTPMLKALKQEYAHKEVYLSMEERMGCGIGACFACVCHTNESETSYV
KVCLDGPVFKAQEVAL
>Mature_256_residues
MKKAYLTVCSNQQIADRVFQMVLKGELVQGFTTPGQFLHLKVSEAVTPLLRRPISIADVNFEKNEVTIIYRVDGEGTRLL
SLKQQGELVDVLGPLGNGFPVNEVQPGKTALLVGGGVGVPPLQELSKRLIEKGVNVIHVLGFQSAKDVFYEEECRQYGDT
YVATADGSYGETGFVTDVIKRKKLEFDILLSCGPTPMLKALKQEYAHKEVYLSMEERMGCGIGACFACVCHTNESETSYV
KVCLDGPVFKAQEVAL

Specific function: Is responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the pyrD subunit to the ultimate electron acceptor NAD(+)

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRK_BACSU (P25983)

Other databases:

- EMBL:   M59757
- EMBL:   AL009126
- PIR:   G39845
- RefSeq:   NP_389436.1
- ProteinModelPortal:   P25983
- SMR:   P25983
- EnsemblBacteria:   EBBACT00000003148
- GeneID:   936854
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15530
- NMPDR:   fig|224308.1.peg.1555
- GenoList:   BSU15530
- GeneTree:   EBGT00050000000799
- HOGENOM:   HBG730970
- OMA:   RPISICS
- ProtClustDB:   PRK00054
- BioCyc:   BSUB:BSU15530-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01211_B
- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- PIRSF:   PIRSF006816

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: NA

Molecular weight: Translated: 28100; Mature: 28100

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAYLTVCSNQQIADRVFQMVLKGELVQGFTTPGQFLHLKVSEAVTPLLRRPISIADVN
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCEEEECC
FEKNEVTIIYRVDGEGTRLLSLKQQGELVDVLGPLGNGFPVNEVQPGKTALLVGGGVGVP
CCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCC
PLQELSKRLIEKGVNVIHVLGFQSAKDVFYEEECRQYGDTYVATADGSYGETGFVTDVIK
CHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHH
RKKLEFDILLSCGPTPMLKALKQEYAHKEVYLSMEERMGCGIGACFACVCHTNESETSYV
HHCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEHHHHCCCCHHHHHHEEECCCCCCCEEE
KVCLDGPVFKAQEVAL
EEEECCCCCCCHHCCC
>Mature Secondary Structure
MKKAYLTVCSNQQIADRVFQMVLKGELVQGFTTPGQFLHLKVSEAVTPLLRRPISIADVN
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCEEEECC
FEKNEVTIIYRVDGEGTRLLSLKQQGELVDVLGPLGNGFPVNEVQPGKTALLVGGGVGVP
CCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCC
PLQELSKRLIEKGVNVIHVLGFQSAKDVFYEEECRQYGDTYVATADGSYGETGFVTDVIK
CHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHH
RKKLEFDILLSCGPTPMLKALKQEYAHKEVYLSMEERMGCGIGACFACVCHTNESETSYV
HHCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEHHHHCCCCHHHHHHEEECCCCCCCEEE
KVCLDGPVFKAQEVAL
EEEECCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1709162; 9384377; 8759868; 10545205