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The map label for this gene is sigG

Identifier: 16078597

GI number: 16078597

Start: 1605630

End: 1606412

Strand: Direct

Name: sigG

Synonym: BSU15330

Alternate gene names: 16078597

Gene position: 1605630-1606412 (Clockwise)

Preceding gene: 255767354

Following gene: 255767355

Centisome position: 38.09

GC content: 42.78

Gene sequence:

>783_bases
GTGTCGAGAAATAAAGTCGAAATCTGCGGGGTGGATACCTCCAAATTACCAGTACTCAAGAATGAAGAGATGAGAAAGCT
GTTTAGGCAGCTGCAGGATGAAGGCGATGATTCAGCAAGAGAAAAGCTTGTAAACGGGAACTTGCGTCTTGTCTTAAGTG
TCATTCAACGATTTAATAACAGAGGGGAGTATGTTGATGACTTATTTCAAGTCGGCTGCATCGGACTAATGAAATCCATT
GATAATTTTGACCTAAGCCACAATGTTAAGTTTTCAACATACGCTGTACCTATGATTATCGGAGAGATCCGCAGGTATTT
ACGTGATAATAACCCGATCCGTGTCTCAAGATCACTCCGGGACATCGCTTACAAGGCGCTTCAGGTAAGAGAGCGGTTAA
TCAGTGAGACAAGCAAGGAGCCGACTGCAGAAGACATCGCAAAAGTTCTTGAAGTACCGCATGAGGAAATTGTATTTGCG
CTTGATGCCATTCAGGATCCGGTTTCTCTATTTGAACCGATCTATAACGACGGCGGAGACCCGATCTATGTGATGGACCA
AATCAGTGATGAGCGTAATACAGATTCACAATGGATTGAAGAGCTGGCTTTAAAAGAGGGCATGAGAAGGCTGAACGATA
GGGAAAAAATGATTTTGAGAAAGCGATTCTTCCAAGGGAAAACCCAAATGGAAGTCGCCGAGGAAATCGGAATTTCTCAA
GCGCAGGTGTCCAGACTTGAAAAAGCGGCTATCAAACAAATGAATAAAAATATTCATCAATAA

Upstream 100 bases:

>100_bases
TCCTGATTTCGACAGTTTTTCGGTCTGAGTGCAGTGCATATTTTTCCCACCCAAGGAGATACTTAACGTTGTACAGCAGC
TCCTGTAGGGAGGGAAAAAA

Downstream 100 bases:

>100_bases
TGAAAAGCCTTTAAAACGATGTTGTTTTAAAGGCTTTTCTATTGATTATGGATGGTTTACGTTTTGGAAAAAGTAAGGTA
TCCTAGTTCGTACAAAGTTT

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSRNKVEICGVDTSKLPVLKNEEMRKLFRQLQDEGDDSAREKLVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSI
DNFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLISETSKEPTAEDIAKVLEVPHEEIVFA
LDAIQDPVSLFEPIYNDGGDPIYVMDQISDERNTDSQWIEELALKEGMRRLNDREKMILRKRFFQGKTQMEVAEEIGISQ
AQVSRLEKAAIKQMNKNIHQ

Sequences:

>Translated_260_residues
MSRNKVEICGVDTSKLPVLKNEEMRKLFRQLQDEGDDSAREKLVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSI
DNFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLISETSKEPTAEDIAKVLEVPHEEIVFA
LDAIQDPVSLFEPIYNDGGDPIYVMDQISDERNTDSQWIEELALKEGMRRLNDREKMILRKRFFQGKTQMEVAEEIGISQ
AQVSRLEKAAIKQMNKNIHQ
>Mature_259_residues
SRNKVEICGVDTSKLPVLKNEEMRKLFRQLQDEGDDSAREKLVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSID
NFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLISETSKEPTAEDIAKVLEVPHEEIVFAL
DAIQDPVSLFEPIYNDGGDPIYVMDQISDERNTDSQWIEELALKEGMRRLNDREKMILRKRFFQGKTQMEVAEEIGISQA
QVSRLEKAAIKQMNKNIHQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=30.9734513274336, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789098, Length=253, Percent_Identity=26.4822134387352, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789871, Length=262, Percent_Identity=27.4809160305344, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): RPSG_BACSU (P19940)

Other databases:

- EMBL:   X57547
- EMBL:   J04077
- EMBL:   AL009126
- PIR:   A30171
- RefSeq:   NP_389416.1
- ProteinModelPortal:   P19940
- SMR:   P19940
- EnsemblBacteria:   EBBACT00000000547
- GeneID:   939953
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15330
- NMPDR:   fig|224308.1.peg.1535
- GenoList:   BSU15330
- GeneTree:   EBGT00070000031820
- HOGENOM:   HBG745096
- OMA:   QISDERN
- ProtClustDB:   PRK08215
- BioCyc:   BSUB:BSU15330-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02980
- TIGRFAMs:   TIGR02937
- TIGRFAMs:   TIGR02850

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 30074; Mature: 29943

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNKVEICGVDTSKLPVLKNEEMRKLFRQLQDEGDDSAREKLVNGNLRLVLSVIQRFNN
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCC
RGEYVDDLFQVGCIGLMKSIDNFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
DIAYKALQVRERLISETSKEPTAEDIAKVLEVPHEEIVFALDAIQDPVSLFEPIYNDGGD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHCCCCC
PIYVMDQISDERNTDSQWIEELALKEGMRRLNDREKMILRKRFFQGKTQMEVAEEIGISQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
AQVSRLEKAAIKQMNKNIHQ
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRNKVEICGVDTSKLPVLKNEEMRKLFRQLQDEGDDSAREKLVNGNLRLVLSVIQRFNN
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCC
RGEYVDDLFQVGCIGLMKSIDNFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
DIAYKALQVRERLISETSKEPTAEDIAKVLEVPHEEIVFALDAIQDPVSLFEPIYNDGGD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHCCCCC
PIYVMDQISDERNTDSQWIEELALKEGMRRLNDREKMILRKRFFQGKTQMEVAEEIGISQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
AQVSRLEKAAIKQMNKNIHQ
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377