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The map label for this gene is murB

Identifier: 16078587

GI number: 16078587

Start: 1592663

End: 1593574

Strand: Direct

Name: murB

Synonym: BSU15230

Alternate gene names: 16078587

Gene position: 1592663-1593574 (Clockwise)

Preceding gene: 255767349

Following gene: 16078588

Centisome position: 37.78

GC content: 46.27

Gene sequence:

>912_bases
ATGGAGAAAGTGATACAGGAATTAAAAGAACGAGAAGTCGGCAAGGTTCTTGCAAATGAACCGCTCGCGAATCATACAAC
GATGAAAATCGGCGGACCTGCGGATGTATTGGTCATTCCAAGCAGTGTGGATGCTGTTAAAGACATCATGGACGTGATTA
AAAAATATGATGTGAAGTGGACAGTCATCGGCCGCGGATCAAATCTTCTTGTTTTAGATGAAGGAATTAGAGGCGTAGTG
ATCAAGCTGGGGGCGGGTCTTGACCATTTGGAGCTTGAAGGGGAACAAGTAACAGTCGGAGGCGGTTATTCGGTGGTCCG
CCTTGCTACATCATTGAGCAAAAAAGGCCTGTCCGGTTTGGAATTTGCTGCCGGCATTCCAGGATCAGTCGGCGGAGCGG
TTTATATGAATGCCGGCGCTCACGGTTCTGATATGAGCGAGATCCTCGTCAAAGCGCATATTTTATTTGAAGACGGAACG
ATTGAGTGGCTGACAAATGAGCAGATGGATTTCAGCTACAGGACATCTGTACTGCAAAAGAAACGCCCGGGCGTTTGCCT
TGAAGCTGTTTTGCAGCTTGAGCAGAAGGATAAGGAATCAATCGTTCAGCAAATGCAAAGCAATAAAGACTATAGAAAAA
ATACACAGCCGTATTCAAGCCCTTGTGCGGGAAGCATATTCAGAAATCCGCTTCCGAATCATGCCGGGAACCTTGTAGAA
AAAGCAGGCTTGAAAGGATATCAAATCGGCGGTGCAAAGATATCGGAGATGCACGGAAACTTCATCGTCAATGCGGGGGG
AGCATCAGCAAAAGATGTGCTTGATCTCATTGACCATGTGAAAAAGACAATCCGTGAAAAATACGAGATTGATATGCACA
CAGAGGTTGAAATCATCGGCGGAAATCGCTGA

Upstream 100 bases:

>100_bases
ACAAAGTCACTCGGTGTTCCTGACGCGGCTGCCCGTTTATACAGCGTACTCGAGGAATTAAAAAAATAGTAGAAAAGCAA
ATGGAATGCGGAGGTTTACG

Downstream 100 bases:

>100_bases
TTCAAGTTCTGACTGAAGCTGTTCATATGATATACTGTAAGCAAACGACAAACGGCATCATAGTATGCCGTTTGTTTTGG
AATAGACAGACTTTTAACAG

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MEKVIQELKEREVGKVLANEPLANHTTMKIGGPADVLVIPSSVDAVKDIMDVIKKYDVKWTVIGRGSNLLVLDEGIRGVV
IKLGAGLDHLELEGEQVTVGGGYSVVRLATSLSKKGLSGLEFAAGIPGSVGGAVYMNAGAHGSDMSEILVKAHILFEDGT
IEWLTNEQMDFSYRTSVLQKKRPGVCLEAVLQLEQKDKESIVQQMQSNKDYRKNTQPYSSPCAGSIFRNPLPNHAGNLVE
KAGLKGYQIGGAKISEMHGNFIVNAGGASAKDVLDLIDHVKKTIREKYEIDMHTEVEIIGGNR

Sequences:

>Translated_303_residues
MEKVIQELKEREVGKVLANEPLANHTTMKIGGPADVLVIPSSVDAVKDIMDVIKKYDVKWTVIGRGSNLLVLDEGIRGVV
IKLGAGLDHLELEGEQVTVGGGYSVVRLATSLSKKGLSGLEFAAGIPGSVGGAVYMNAGAHGSDMSEILVKAHILFEDGT
IEWLTNEQMDFSYRTSVLQKKRPGVCLEAVLQLEQKDKESIVQQMQSNKDYRKNTQPYSSPCAGSIFRNPLPNHAGNLVE
KAGLKGYQIGGAKISEMHGNFIVNAGGASAKDVLDLIDHVKKTIREKYEIDMHTEVEIIGGNR
>Mature_303_residues
MEKVIQELKEREVGKVLANEPLANHTTMKIGGPADVLVIPSSVDAVKDIMDVIKKYDVKWTVIGRGSNLLVLDEGIRGVV
IKLGAGLDHLELEGEQVTVGGGYSVVRLATSLSKKGLSGLEFAAGIPGSVGGAVYMNAGAHGSDMSEILVKAHILFEDGT
IEWLTNEQMDFSYRTSVLQKKRPGVCLEAVLQLEQKDKESIVQQMQSNKDYRKNTQPYSSPCAGSIFRNPLPNHAGNLVE
KAGLKGYQIGGAKISEMHGNFIVNAGGASAKDVLDLIDHVKKTIREKYEIDMHTEVEIIGGNR

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=288, Percent_Identity=27.0833333333333, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_BACSU (P18579)

Other databases:

- EMBL:   M31827
- EMBL:   AL009126
- EMBL:   D10602
- EMBL:   M31800
- PIR:   S26500
- RefSeq:   NP_389406.1
- ProteinModelPortal:   P18579
- SMR:   P18579
- EnsemblBacteria:   EBBACT00000004024
- GeneID:   939804
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15230
- NMPDR:   fig|224308.1.peg.1525
- GenoList:   BSU15230
- GeneTree:   EBGT00050000001391
- HOGENOM:   HBG686573
- OMA:   LESSKIP
- PhylomeDB:   P18579
- ProtClustDB:   PRK13905
- BioCyc:   BSUB:BSU15230-MONOMER
- BRENDA:   1.1.1.158
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 32808; Mature: 32808

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 174-174 ACT_SITE 225-225 ACT_SITE 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVIQELKEREVGKVLANEPLANHTTMKIGGPADVLVIPSSVDAVKDIMDVIKKYDVKW
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCEE
TVIGRGSNLLVLDEGIRGVVIKLGAGLDHLELEGEQVTVGGGYSVVRLATSLSKKGLSGL
EEEECCCCEEEEECCCCEEEEEECCCCCCEEECCCEEEECCCHHHHHHHHHHHHCCCCCC
EFAAGIPGSVGGAVYMNAGAHGSDMSEILVKAHILFEDGTIEWLTNEQMDFSYRTSVLQK
HHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHEEEEECCEEEEECCCCCCHHHHHHHHHH
KRPGVCLEAVLQLEQKDKESIVQQMQSNKDYRKNTQPYSSPCAGSIFRNPLPNHAGNLVE
CCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHCCCCCCHHHHHHH
KAGLKGYQIGGAKISEMHGNFIVNAGGASAKDVLDLIDHVKKTIREKYEIDMHTEVEIIG
HCCCCCEEECCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GNR
CCC
>Mature Secondary Structure
MEKVIQELKEREVGKVLANEPLANHTTMKIGGPADVLVIPSSVDAVKDIMDVIKKYDVKW
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCEE
TVIGRGSNLLVLDEGIRGVVIKLGAGLDHLELEGEQVTVGGGYSVVRLATSLSKKGLSGL
EEEECCCCEEEEECCCCEEEEEECCCCCCEEECCCEEEECCCHHHHHHHHHHHHCCCCCC
EFAAGIPGSVGGAVYMNAGAHGSDMSEILVKAHILFEDGTIEWLTNEQMDFSYRTSVLQK
HHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHEEEEECCEEEEECCCCCCHHHHHHHHHH
KRPGVCLEAVLQLEQKDKESIVQQMQSNKDYRKNTQPYSSPCAGSIFRNPLPNHAGNLVE
CCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHCCCCCCHHHHHHH
KAGLKGYQIGGAKISEMHGNFIVNAGGASAKDVLDLIDHVKKTIREKYEIDMHTEVEIIG
HCCCCCEEECCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GNR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2556375; 9384377; 1387377; 2556376; 7590298