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The map label for this gene is mraW

Identifier: 16078578

GI number: 16078578

Start: 1580622

End: 1581557

Strand: Direct

Name: mraW

Synonym: BSU15140

Alternate gene names: 16078578

Gene position: 1580622-1581557 (Clockwise)

Preceding gene: 16078577

Following gene: 16078579

Centisome position: 37.49

GC content: 45.73

Gene sequence:

>936_bases
ATGTTTCAACACAAGACAGTACTTCTTCGTGAAACCGTAGACGGACTTAATATTAAACCAGACGGTACATATGTAGACTG
CACACTCGGCGGAGCTGGGCATAGTACATACTTATTGCAGCAGTTATCCGAGAAGGGGCGTTTAATCGCTTTTGACCAAG
ATGATACGGCATTACAGCATGCTAAAGAAGTGTTGTCCGATTATAAAGGACAGCTCATTCTGATTAAAAGCAATTTCAGG
TATTTAAAAGAATATTTGAATGAACAAGGCGTTACAGAAGTAGACGGCATTTTATTTGATTTAGGGGTGTCCTCTCCGCA
GCTGGATACACCGGAGCGCGGATTCAGTTACCATCATGACGCACCGTTGGACATGAGGATGGACCAGTCGGCTACGCTTT
CGGCGAAAGAAGTCGTTAATGAGTGGCGCTATGAGGATCTCGTCCGTATTTTCTTTAAATACGGAGAAGAGAAGTTCAGT
AAACAGATCGCCAGAAAAATTGAGGAGGCAAGAATGAAGTCTCCTATTCAAACTACGGGCCAACTGGTCGATCTAATAAA
AGACGCGATTCCCGCTCCAGCGAGAAGGAGCGGGGGACATCCCGCTAAACGCGTGTTTCAGGCAATCAGAATTGCCGTAA
ACGATGAACTTAGGGTGTTTGAAGAAGCTTTGGAGCAGGCAATTGAGGTTCTGAAGCCAGGGGGCAGGGTATCGGTCATT
ACCTTCCACTCGCTAGAAGACAGAATTTGCAAAACCACTTTTAAAGAAAAGTCGTCGCTTCCGGAACTTCCTCCGGGACT
TCCTGTTATACCGGAAGAGTTTGAACCGGAGCTGAAGCTCATCACACGAAAACCGATTACGGCATCTCAAGAAGAGCTTG
AAGAGAACAACCGGGCTCGTTCTGCCAAGCTTCGGATTGCTGAAAAAAGAAAATAA

Upstream 100 bases:

>100_bases
TGCTGAAAACATGATTGGGTTTGATATATAATGATTCTTCGTGTATAACACTCTAAAAACAATTTCAATTCATCCTGATA
AAAGGTGGGACCAACACAAT

Downstream 100 bases:

>100_bases
CGAAACGCGAACGCAAAATTAAAAGGAGGTCATCAGCCTATGAGCAATTTAGCTTACCAACCAGAGAAACAGCAGCGGCA
TGCGATCAGTCCTGAGAAAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQHAKEVLSDYKGQLILIKSNFR
YLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFS
KQIARKIEEARMKSPIQTTGQLVDLIKDAIPAPARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVI
TFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRARSAKLRIAEKRK

Sequences:

>Translated_311_residues
MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQHAKEVLSDYKGQLILIKSNFR
YLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFS
KQIARKIEEARMKSPIQTTGQLVDLIKDAIPAPARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVI
TFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRARSAKLRIAEKRK
>Mature_311_residues
MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQHAKEVLSDYKGQLILIKSNFR
YLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFS
KQIARKIEEARMKSPIQTTGQLVDLIKDAIPAPARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVI
TFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRARSAKLRIAEKRK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=36.2017804154303, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=38.9473684210526, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=45.859872611465, Blast_Score=261, Evalue=3e-71,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=38.1818181818182, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=38.1818181818182, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35306; Mature: 35306

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQH
CCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHH
AKEVLSDYKGQLILIKSNFRYLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHD
HHHHHHHCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFSKQIARKIEEARMKSPIQTTG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
QLVDLIKDAIPAPARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRAR
EECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHH
SAKLRIAEKRK
HHHHHHHCCCC
>Mature Secondary Structure
MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQH
CCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHH
AKEVLSDYKGQLILIKSNFRYLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHD
HHHHHHHCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFSKQIARKIEEARMKSPIQTTG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
QLVDLIKDAIPAPARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRAR
EECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHH
SAKLRIAEKRK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA