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The map label for this gene is def

Identifier: 16078520

GI number: 16078520

Start: 1526195

End: 1526749

Strand: Reverse

Name: def

Synonym: BSU14560

Alternate gene names: 16078520

Gene position: 1526749-1526195 (Counterclockwise)

Preceding gene: 255767336

Following gene: 16078518

Centisome position: 36.22

GC content: 43.6

Gene sequence:

>555_bases
TTGATTACTATGGAAAACATCGTACGTGACGGCCATCCAGCCCTCAGAGAGACTGCCGAACCTGTTGAGCTGCCTCCAAC
TGATGCAGAAAAACAGCAGCTGGCCGACATGATCGAATTTGTTAAAAACAGCCAAAATCCAGAATTAGCCGAAAAATACA
AGCTGCGCCCTGGCGTAGGACTCGCAGCTCCTCAAATTAATATCAAAAAACGAATGATCGCCGTTCATGCGGAAGATGCT
TCCGGTAAATTATACAGCTATGCTCTGTTTAATCCTAAGATCGTCAGCCATTCAGTTGAAAAAAGCTACTTAACAAGCGG
CGAGGGCTGCCTCTCTGTGGATGAAGCGATTCCCGGTTATGTTCCCCGCTATGCTCGCATCAGAGTAAAAGGAACAACAC
TAGAAGGCGAAAATATTGATATTCGTTTAAAAGGATTTCCGGCTATTGTGTTTCAGCATGAGATCGATCATTTGAACGGC
GTTATGTTCTATGATCATATTGATAAGGAAAATCCTTTCAAAGAACCTGAAAATGCAATCGCAATTGAGCGCTAA

Upstream 100 bases:

>100_bases
ATGAAATTGACCAGCCATATACGGCTGGTCATTCGTTTCGTTGCACCTTTGTGAAGAAATCCGATATAATTTACATATTA
AGCTTTAGGAGTGAAGTTTC

Downstream 100 bases:

>100_bases
AAGAGAAAACGGCTGGCACAATGCCAGCCGTTTCTTTATTTTAAAAGGCCCAATTCTTTGACAGCGTATGCAATTCCATC
CTCGTCCACCGGTTTTGTTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MITMENIVRDGHPALRETAEPVELPPTDAEKQQLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQINIKKRMIAVHAEDA
SGKLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEAIPGYVPRYARIRVKGTTLEGENIDIRLKGFPAIVFQHEIDHLNG
VMFYDHIDKENPFKEPENAIAIER

Sequences:

>Translated_184_residues
MITMENIVRDGHPALRETAEPVELPPTDAEKQQLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQINIKKRMIAVHAEDA
SGKLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEAIPGYVPRYARIRVKGTTLEGENIDIRLKGFPAIVFQHEIDHLNG
VMFYDHIDKENPFKEPENAIAIER
>Mature_184_residues
MITMENIVRDGHPALRETAEPVELPPTDAEKQQLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQINIKKRMIAVHAEDA
SGKLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEAIPGYVPRYARIRVKGTTLEGENIDIRLKGFPAIVFQHEIDHLNG
VMFYDHIDKENPFKEPENAIAIER

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=161, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24645728, Length=174, Percent_Identity=30.4597701149425, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_BACSU (Q45495)

Other databases:

- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   D69862
- RefSeq:   NP_389339.1
- ProteinModelPortal:   Q45495
- SMR:   Q45495
- EnsemblBacteria:   EBBACT00000001939
- GeneID:   939491
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14560
- NMPDR:   fig|224308.1.peg.1458
- GenoList:   BSU14560
- GeneTree:   EBGT00050000001175
- HOGENOM:   HBG665227
- OMA:   DIIKEGH
- ProtClustDB:   PRK00150
- BioCyc:   BSUB:BSU14560-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20656; Mature: 20656

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMENIVRDGHPALRETAEPVELPPTDAEKQQLADMIEFVKNSQNPELAEKYKLRPGVG
CCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
LAAPQINIKKRMIAVHAEDASGKLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEAIPGY
CCCCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEHHHCCCCC
VPRYARIRVKGTTLEGENIDIRLKGFPAIVFQHEIDHLNGVMFYDHIDKENPFKEPENAI
CCCEEEEEEEEEEECCCCEEEEECCCEEEEEEECHHHCCCEEEEECCCCCCCCCCCCCCE
AIER
EECC
>Mature Secondary Structure
MITMENIVRDGHPALRETAEPVELPPTDAEKQQLADMIEFVKNSQNPELAEKYKLRPGVG
CCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
LAAPQINIKKRMIAVHAEDASGKLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEAIPGY
CCCCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEHHHCCCCC
VPRYARIRVKGTTLEGENIDIRLKGFPAIVFQHEIDHLNGVMFYDHIDKENPFKEPENAI
CCCEEEEEEEEEEECCCCEEEEECCCEEEEEEECHHHCCCEEEEECCCCCCCCCCCCCCE
AIER
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11429456